Differential chromatin binding preference is the result of the neo-functionalization of the TB1 clade of TCP transcription factors in grasses

Autores
Mansilla, Natanael; Fonouni-farde, Camille Audrey; Ariel, Federico Damian; Lucero, Leandro Exequiel
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The understanding of neo-functionalization of plant transcription factors (TFs) after gene duplication has been extensively focused on changes in protein–protein interactions, the expression pattern of TFs, or the variation of cis-elements bound by TFs. Yet, the main molecular role of a TF, that is, its specific chromatin binding for the direct regulation of target gene expression, continues to be mostly overlooked. Here, we studied the TB1 clade of the TEOSINTE BRANCHED 1, CYCLOIDEA, PROLIFERATING CELL FACTORS (TCP) TF family within the grasses (Poaceae). We identified an Asp/Gly amino acid replacement within the TCP domain, originated within a paralog TIG1 clade exclusive for grasses. The heterologous expression of Zea mays TB1 and its two paralogs BAD1 and TIG1 in Arabidopsis mutant plants lacking the TB1 ortholog BRC1 revealed distinct functions in plant development. Notably, the Gly acquired in the TIG1 clade does not impair TF homodimerization and heterodimerization, while it modulates chromatin binding preferences. We found that in vivo TF recognition of target promoters depends on this Asp/Gly mutation and directly impacts downstream gene expression and subsequent plant development. These results provided new insights into how natural selection fine-tunes gene expression regulation after duplication of TFs to define plant architecture.
Fil: Mansilla, Natanael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Fonouni-farde, Camille Audrey. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Lucero, Leandro Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Materia
CHROMATIN BINDING PREFERENCE
GENE NEO-FUNCTIONALIZATION
GRASSES
TB1
TCP TRANSCRIPTION FACTORS
ZEA MAYS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/212766
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/212766
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential chromatin binding preference is the result of the neo-functionalization of the TB1 clade of TCP transcription factors in grassesMansilla, NatanaelFonouni-farde, Camille AudreyAriel, Federico DamianLucero, Leandro ExequielCHROMATIN BINDING PREFERENCEGENE NEO-FUNCTIONALIZATIONGRASSESTB1TCP TRANSCRIPTION FACTORSZEA MAYShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The understanding of neo-functionalization of plant transcription factors (TFs) after gene duplication has been extensively focused on changes in protein–protein interactions, the expression pattern of TFs, or the variation of cis-elements bound by TFs. Yet, the main molecular role of a TF, that is, its specific chromatin binding for the direct regulation of target gene expression, continues to be mostly overlooked. Here, we studied the TB1 clade of the TEOSINTE BRANCHED 1, CYCLOIDEA, PROLIFERATING CELL FACTORS (TCP) TF family within the grasses (Poaceae). We identified an Asp/Gly amino acid replacement within the TCP domain, originated within a paralog TIG1 clade exclusive for grasses. The heterologous expression of Zea mays TB1 and its two paralogs BAD1 and TIG1 in Arabidopsis mutant plants lacking the TB1 ortholog BRC1 revealed distinct functions in plant development. Notably, the Gly acquired in the TIG1 clade does not impair TF homodimerization and heterodimerization, while it modulates chromatin binding preferences. We found that in vivo TF recognition of target promoters depends on this Asp/Gly mutation and directly impacts downstream gene expression and subsequent plant development. These results provided new insights into how natural selection fine-tunes gene expression regulation after duplication of TFs to define plant architecture.Fil: Mansilla, Natanael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Fonouni-farde, Camille Audrey. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Lucero, Leandro Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaWiley Blackwell Publishing, Inc2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/212766Mansilla, Natanael; Fonouni-farde, Camille Audrey; Ariel, Federico Damian; Lucero, Leandro Exequiel; Differential chromatin binding preference is the result of the neo-functionalization of the TB1 clade of TCP transcription factors in grasses; Wiley Blackwell Publishing, Inc; New Phytologist; 237; 6; 12-2022; 2088-21030028-646XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/nph.18664info:eu-repo/semantics/altIdentifier/doi/10.1111/nph.18664info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-06-10T10:07:38Zoai:ri.conicet.gov.ar:11336/212766instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-06-10 10:07:38.529CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential chromatin binding preference is the result of the neo-functionalization of the TB1 clade of TCP transcription factors in grasses
title Differential chromatin binding preference is the result of the neo-functionalization of the TB1 clade of TCP transcription factors in grasses
spellingShingle Differential chromatin binding preference is the result of the neo-functionalization of the TB1 clade of TCP transcription factors in grasses
Mansilla, Natanael
CHROMATIN BINDING PREFERENCE
GENE NEO-FUNCTIONALIZATION
GRASSES
TB1
TCP TRANSCRIPTION FACTORS
ZEA MAYS
title_short Differential chromatin binding preference is the result of the neo-functionalization of the TB1 clade of TCP transcription factors in grasses
title_full Differential chromatin binding preference is the result of the neo-functionalization of the TB1 clade of TCP transcription factors in grasses
title_fullStr Differential chromatin binding preference is the result of the neo-functionalization of the TB1 clade of TCP transcription factors in grasses
title_full_unstemmed Differential chromatin binding preference is the result of the neo-functionalization of the TB1 clade of TCP transcription factors in grasses
title_sort Differential chromatin binding preference is the result of the neo-functionalization of the TB1 clade of TCP transcription factors in grasses
dc.creator.none.fl_str_mv Mansilla, Natanael
Fonouni-farde, Camille Audrey
Ariel, Federico Damian
Lucero, Leandro Exequiel
author Mansilla, Natanael
author_facet Mansilla, Natanael
Fonouni-farde, Camille Audrey
Ariel, Federico Damian
Lucero, Leandro Exequiel
author_role author
author2 Fonouni-farde, Camille Audrey
Ariel, Federico Damian
Lucero, Leandro Exequiel
author2_role author
author
author
dc.subject.none.fl_str_mv CHROMATIN BINDING PREFERENCE
GENE NEO-FUNCTIONALIZATION
GRASSES
TB1
TCP TRANSCRIPTION FACTORS
ZEA MAYS
topic CHROMATIN BINDING PREFERENCE
GENE NEO-FUNCTIONALIZATION
GRASSES
TB1
TCP TRANSCRIPTION FACTORS
ZEA MAYS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The understanding of neo-functionalization of plant transcription factors (TFs) after gene duplication has been extensively focused on changes in protein–protein interactions, the expression pattern of TFs, or the variation of cis-elements bound by TFs. Yet, the main molecular role of a TF, that is, its specific chromatin binding for the direct regulation of target gene expression, continues to be mostly overlooked. Here, we studied the TB1 clade of the TEOSINTE BRANCHED 1, CYCLOIDEA, PROLIFERATING CELL FACTORS (TCP) TF family within the grasses (Poaceae). We identified an Asp/Gly amino acid replacement within the TCP domain, originated within a paralog TIG1 clade exclusive for grasses. The heterologous expression of Zea mays TB1 and its two paralogs BAD1 and TIG1 in Arabidopsis mutant plants lacking the TB1 ortholog BRC1 revealed distinct functions in plant development. Notably, the Gly acquired in the TIG1 clade does not impair TF homodimerization and heterodimerization, while it modulates chromatin binding preferences. We found that in vivo TF recognition of target promoters depends on this Asp/Gly mutation and directly impacts downstream gene expression and subsequent plant development. These results provided new insights into how natural selection fine-tunes gene expression regulation after duplication of TFs to define plant architecture.
Fil: Mansilla, Natanael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Fonouni-farde, Camille Audrey. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Lucero, Leandro Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
description The understanding of neo-functionalization of plant transcription factors (TFs) after gene duplication has been extensively focused on changes in protein–protein interactions, the expression pattern of TFs, or the variation of cis-elements bound by TFs. Yet, the main molecular role of a TF, that is, its specific chromatin binding for the direct regulation of target gene expression, continues to be mostly overlooked. Here, we studied the TB1 clade of the TEOSINTE BRANCHED 1, CYCLOIDEA, PROLIFERATING CELL FACTORS (TCP) TF family within the grasses (Poaceae). We identified an Asp/Gly amino acid replacement within the TCP domain, originated within a paralog TIG1 clade exclusive for grasses. The heterologous expression of Zea mays TB1 and its two paralogs BAD1 and TIG1 in Arabidopsis mutant plants lacking the TB1 ortholog BRC1 revealed distinct functions in plant development. Notably, the Gly acquired in the TIG1 clade does not impair TF homodimerization and heterodimerization, while it modulates chromatin binding preferences. We found that in vivo TF recognition of target promoters depends on this Asp/Gly mutation and directly impacts downstream gene expression and subsequent plant development. These results provided new insights into how natural selection fine-tunes gene expression regulation after duplication of TFs to define plant architecture.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/212766
Mansilla, Natanael; Fonouni-farde, Camille Audrey; Ariel, Federico Damian; Lucero, Leandro Exequiel; Differential chromatin binding preference is the result of the neo-functionalization of the TB1 clade of TCP transcription factors in grasses; Wiley Blackwell Publishing, Inc; New Phytologist; 237; 6; 12-2022; 2088-2103
0028-646X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/212766
identifier_str_mv Mansilla, Natanael; Fonouni-farde, Camille Audrey; Ariel, Federico Damian; Lucero, Leandro Exequiel; Differential chromatin binding preference is the result of the neo-functionalization of the TB1 clade of TCP transcription factors in grasses; Wiley Blackwell Publishing, Inc; New Phytologist; 237; 6; 12-2022; 2088-2103
0028-646X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/nph.18664
info:eu-repo/semantics/altIdentifier/doi/10.1111/nph.18664
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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