In Vivo and In Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach

Autores
Mateo Fernández, Marcos; Merinas Amo, Tania; Moreno Millán, Miguel; Alonso Moraga, Ángeles; Demyda-peyrás, Sebastian
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to assess the biological and food safety of two different beverages: Classic Coca Cola (CCC) and CaffeineFree Coca Cola (CFCC). To this end, we determined the genotoxicological and biological effects of different doses of lyophilised CCC and CFCC and Caffeine (CAF), the main distinctive constituent. Their toxic/antitoxic, genotoxic/antigenotoxic, and chronic toxicity (lifespan assay) effects were determined in vivo using the Drosophila model. Their cytotoxic activities were determined using the HL-60 in vitro cancer model. In addition, clastogenic DNA toxicity was measured using internucleosomal fragmentation and SCGE assays. Their epigenetic effects were assessed on the HL-60 methylation status using some repetitive elements. The experimental results showed a slight chemopreventive effect of the two cola beverages against HL-60 leukaemia cells, probably mediated by nonapoptotic mechanisms. Finally, CCC and CAF induced a global genome hypomethylation evaluated in LINE-1 and Alu M1 repetitive elements. Overall, we demonstrated for the first time the safety of this famous beverage in in vivo and in vitro models.
Fil: Mateo Fernández, Marcos. Universidad de Córdoba. Departamento de Genética. Córdoba; España
Fil: Merinas Amo, Tania. Universidad de Córdoba. Departamento de Genética. Córdoba; España
Fil: Moreno Millán, Miguel. Universidad de Córdoba. Departamento de Genética. Córdoba; España
Fil: Alonso Moraga, Ángeles. Universidad de Córdoba. Departamento de Genética. Córdoba; España
Fil: Demyda-peyrás, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Materia
Coca Cola
gentoxicity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25034
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/25034
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In Vivo and In Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay ApproachMateo Fernández, MarcosMerinas Amo, TaniaMoreno Millán, MiguelAlonso Moraga, ÁngelesDemyda-peyrás, SebastianCoca Colagentoxicityhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The aim of this work was to assess the biological and food safety of two different beverages: Classic Coca Cola (CCC) and CaffeineFree Coca Cola (CFCC). To this end, we determined the genotoxicological and biological effects of different doses of lyophilised CCC and CFCC and Caffeine (CAF), the main distinctive constituent. Their toxic/antitoxic, genotoxic/antigenotoxic, and chronic toxicity (lifespan assay) effects were determined in vivo using the Drosophila model. Their cytotoxic activities were determined using the HL-60 in vitro cancer model. In addition, clastogenic DNA toxicity was measured using internucleosomal fragmentation and SCGE assays. Their epigenetic effects were assessed on the HL-60 methylation status using some repetitive elements. The experimental results showed a slight chemopreventive effect of the two cola beverages against HL-60 leukaemia cells, probably mediated by nonapoptotic mechanisms. Finally, CCC and CAF induced a global genome hypomethylation evaluated in LINE-1 and Alu M1 repetitive elements. Overall, we demonstrated for the first time the safety of this famous beverage in in vivo and in vitro models.Fil: Mateo Fernández, Marcos. Universidad de Córdoba. Departamento de Genética. Córdoba; EspañaFil: Merinas Amo, Tania. Universidad de Córdoba. Departamento de Genética. Córdoba; EspañaFil: Moreno Millán, Miguel. Universidad de Córdoba. Departamento de Genética. Córdoba; EspañaFil: Alonso Moraga, Ángeles. Universidad de Córdoba. Departamento de Genética. Córdoba; EspañaFil: Demyda-peyrás, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaHindawi Publishing Corporation2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25034Mateo Fernández, Marcos; Merinas Amo, Tania; Moreno Millán, Miguel; Alonso Moraga, Ángeles; Demyda-peyrás, Sebastian; In Vivo and In Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach; Hindawi Publishing Corporation; BioMed Research International; 2016; 5-2016; 1-152314-61332314-6141CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1155/2016/7574843info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2016/7574843/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:15Zoai:ri.conicet.gov.ar:11336/25034instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:15.445CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In Vivo and In Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach
title In Vivo and In Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach
spellingShingle In Vivo and In Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach
Mateo Fernández, Marcos
Coca Cola
gentoxicity
title_short In Vivo and In Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach
title_full In Vivo and In Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach
title_fullStr In Vivo and In Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach
title_full_unstemmed In Vivo and In Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach
title_sort In Vivo and In Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach
dc.creator.none.fl_str_mv Mateo Fernández, Marcos
Merinas Amo, Tania
Moreno Millán, Miguel
Alonso Moraga, Ángeles
Demyda-peyrás, Sebastian
author Mateo Fernández, Marcos
author_facet Mateo Fernández, Marcos
Merinas Amo, Tania
Moreno Millán, Miguel
Alonso Moraga, Ángeles
Demyda-peyrás, Sebastian
author_role author
author2 Merinas Amo, Tania
Moreno Millán, Miguel
Alonso Moraga, Ángeles
Demyda-peyrás, Sebastian
author2_role author
author
author
author
dc.subject.none.fl_str_mv Coca Cola
gentoxicity
topic Coca Cola
gentoxicity
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of this work was to assess the biological and food safety of two different beverages: Classic Coca Cola (CCC) and CaffeineFree Coca Cola (CFCC). To this end, we determined the genotoxicological and biological effects of different doses of lyophilised CCC and CFCC and Caffeine (CAF), the main distinctive constituent. Their toxic/antitoxic, genotoxic/antigenotoxic, and chronic toxicity (lifespan assay) effects were determined in vivo using the Drosophila model. Their cytotoxic activities were determined using the HL-60 in vitro cancer model. In addition, clastogenic DNA toxicity was measured using internucleosomal fragmentation and SCGE assays. Their epigenetic effects were assessed on the HL-60 methylation status using some repetitive elements. The experimental results showed a slight chemopreventive effect of the two cola beverages against HL-60 leukaemia cells, probably mediated by nonapoptotic mechanisms. Finally, CCC and CAF induced a global genome hypomethylation evaluated in LINE-1 and Alu M1 repetitive elements. Overall, we demonstrated for the first time the safety of this famous beverage in in vivo and in vitro models.
Fil: Mateo Fernández, Marcos. Universidad de Córdoba. Departamento de Genética. Córdoba; España
Fil: Merinas Amo, Tania. Universidad de Córdoba. Departamento de Genética. Córdoba; España
Fil: Moreno Millán, Miguel. Universidad de Córdoba. Departamento de Genética. Córdoba; España
Fil: Alonso Moraga, Ángeles. Universidad de Córdoba. Departamento de Genética. Córdoba; España
Fil: Demyda-peyrás, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
description The aim of this work was to assess the biological and food safety of two different beverages: Classic Coca Cola (CCC) and CaffeineFree Coca Cola (CFCC). To this end, we determined the genotoxicological and biological effects of different doses of lyophilised CCC and CFCC and Caffeine (CAF), the main distinctive constituent. Their toxic/antitoxic, genotoxic/antigenotoxic, and chronic toxicity (lifespan assay) effects were determined in vivo using the Drosophila model. Their cytotoxic activities were determined using the HL-60 in vitro cancer model. In addition, clastogenic DNA toxicity was measured using internucleosomal fragmentation and SCGE assays. Their epigenetic effects were assessed on the HL-60 methylation status using some repetitive elements. The experimental results showed a slight chemopreventive effect of the two cola beverages against HL-60 leukaemia cells, probably mediated by nonapoptotic mechanisms. Finally, CCC and CAF induced a global genome hypomethylation evaluated in LINE-1 and Alu M1 repetitive elements. Overall, we demonstrated for the first time the safety of this famous beverage in in vivo and in vitro models.
publishDate 2016
dc.date.none.fl_str_mv 2016-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25034
Mateo Fernández, Marcos; Merinas Amo, Tania; Moreno Millán, Miguel; Alonso Moraga, Ángeles; Demyda-peyrás, Sebastian; In Vivo and In Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach; Hindawi Publishing Corporation; BioMed Research International; 2016; 5-2016; 1-15
2314-6133
2314-6141
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25034
identifier_str_mv Mateo Fernández, Marcos; Merinas Amo, Tania; Moreno Millán, Miguel; Alonso Moraga, Ángeles; Demyda-peyrás, Sebastian; In Vivo and In Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach; Hindawi Publishing Corporation; BioMed Research International; 2016; 5-2016; 1-15
2314-6133
2314-6141
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1155/2016/7574843
info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2016/7574843/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614516018511872
score 13.070432

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