Age-related differences in the gastrointestinal microbiota of chinstrap penguins (pygoscelis antarctica)

Autores
Barbosa, Andrés; Balagué, Vanessa; Valera, Francisco; Martínez, Ana; Benzal, Jesús; Motas, Miguel; Diaz, Julia Inés; Mira, Alex; Pedrós Alió, Carlos
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The gastrointestinal tract microbiota is known to play very important roles in the well being of animals. It is a complex community composed by hundreds of microbial species interacting closely among them and with their host, that is, a microbial ecosystem. The development of high throughput sequencing techniques allows studying the diversity of such communities in a realistic way and considerable work has been carried out in mammals and some birds such as chickens. Wild birds have received less attention and in particular, in the case of penguins, only a few individuals of five species have been examined with molecular techniques. We collected cloacal samples from Chinstrap penguins in the Vapour Col rookery in Deception Island, Antarctica, and carried out pyrosequencing of the V1-V3 region of the 16S rDNA in samples from 53 individuals, 27 adults and 26 chicks. This provided the first description of the Chinstrap penguin gastrointestinal tract microbiota and the most extensive in any penguin species. Firmicutes, Bacteoridetes, Proteobacteria, Fusobacteria, Actinobacteria, and Tenericutes were the main components. There were large differences between chicks and adults. The former had more Firmicutes and the latter more Bacteroidetes and Proteobacteria. In addition, adults had richer and more diverse bacterial communities than chicks. These differences were also observed between parents and their offspring. On the other hand, nests explained differences in bacterial communities only among chicks. We suggest that environmental factors have a higher importance than genetic factors in the microbiota composition of chicks. The results also showed surprisingly large differences in community composition with other Antarctic penguins including the congeneric Adélie and Gentoo penguins.
Fil: Barbosa, Andrés. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; España
Fil: Balagué, Vanessa. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Fil: Valera, Francisco. Consejo Superior de Investigaciones Científicas. Estación Experimental de Zonas Aridas; España
Fil: Martínez, Ana. Universidad Nacional Autónoma de México; México
Fil: Benzal, Jesús. Consejo Superior de Investigaciones Científicas. Estación Experimental de Zonas Aridas; España
Fil: Motas, Miguel. Universidad de Murcia; España
Fil: Diaz, Julia Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Mira, Alex. Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana; España
Fil: Pedrós Alió, Carlos. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Materia
microbiota
penguin
gastrointestinal tract
pyrsequencing
Diversity
Antarctica
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48998
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Age-related differences in the gastrointestinal microbiota of chinstrap penguins (pygoscelis antarctica)Barbosa, AndrésBalagué, VanessaValera, FranciscoMartínez, AnaBenzal, JesúsMotas, MiguelDiaz, Julia InésMira, AlexPedrós Alió, Carlosmicrobiotapenguingastrointestinal tractpyrsequencingDiversityAntarcticahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The gastrointestinal tract microbiota is known to play very important roles in the well being of animals. It is a complex community composed by hundreds of microbial species interacting closely among them and with their host, that is, a microbial ecosystem. The development of high throughput sequencing techniques allows studying the diversity of such communities in a realistic way and considerable work has been carried out in mammals and some birds such as chickens. Wild birds have received less attention and in particular, in the case of penguins, only a few individuals of five species have been examined with molecular techniques. We collected cloacal samples from Chinstrap penguins in the Vapour Col rookery in Deception Island, Antarctica, and carried out pyrosequencing of the V1-V3 region of the 16S rDNA in samples from 53 individuals, 27 adults and 26 chicks. This provided the first description of the Chinstrap penguin gastrointestinal tract microbiota and the most extensive in any penguin species. Firmicutes, Bacteoridetes, Proteobacteria, Fusobacteria, Actinobacteria, and Tenericutes were the main components. There were large differences between chicks and adults. The former had more Firmicutes and the latter more Bacteroidetes and Proteobacteria. In addition, adults had richer and more diverse bacterial communities than chicks. These differences were also observed between parents and their offspring. On the other hand, nests explained differences in bacterial communities only among chicks. We suggest that environmental factors have a higher importance than genetic factors in the microbiota composition of chicks. The results also showed surprisingly large differences in community composition with other Antarctic penguins including the congeneric Adélie and Gentoo penguins.Fil: Barbosa, Andrés. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; EspañaFil: Balagué, Vanessa. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Valera, Francisco. Consejo Superior de Investigaciones Científicas. Estación Experimental de Zonas Aridas; EspañaFil: Martínez, Ana. Universidad Nacional Autónoma de México; MéxicoFil: Benzal, Jesús. Consejo Superior de Investigaciones Científicas. Estación Experimental de Zonas Aridas; EspañaFil: Motas, Miguel. Universidad de Murcia; EspañaFil: Diaz, Julia Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Mira, Alex. Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana; EspañaFil: Pedrós Alió, Carlos. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaPublic Library of Science2016-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48998Barbosa, Andrés; Balagué, Vanessa; Valera, Francisco; Martínez, Ana; Benzal, Jesús; et al.; Age-related differences in the gastrointestinal microbiota of chinstrap penguins (pygoscelis antarctica); Public Library of Science; Plos One; 11; 4; 4-2016; 1-17; e01532151932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0153215info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0153215info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:08:42Zoai:ri.conicet.gov.ar:11336/48998instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:08:42.416CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Age-related differences in the gastrointestinal microbiota of chinstrap penguins (pygoscelis antarctica)
title Age-related differences in the gastrointestinal microbiota of chinstrap penguins (pygoscelis antarctica)
spellingShingle Age-related differences in the gastrointestinal microbiota of chinstrap penguins (pygoscelis antarctica)
Barbosa, Andrés
microbiota
penguin
gastrointestinal tract
pyrsequencing
Diversity
Antarctica
title_short Age-related differences in the gastrointestinal microbiota of chinstrap penguins (pygoscelis antarctica)
title_full Age-related differences in the gastrointestinal microbiota of chinstrap penguins (pygoscelis antarctica)
title_fullStr Age-related differences in the gastrointestinal microbiota of chinstrap penguins (pygoscelis antarctica)
title_full_unstemmed Age-related differences in the gastrointestinal microbiota of chinstrap penguins (pygoscelis antarctica)
title_sort Age-related differences in the gastrointestinal microbiota of chinstrap penguins (pygoscelis antarctica)
dc.creator.none.fl_str_mv Barbosa, Andrés
Balagué, Vanessa
Valera, Francisco
Martínez, Ana
Benzal, Jesús
Motas, Miguel
Diaz, Julia Inés
Mira, Alex
Pedrós Alió, Carlos
author Barbosa, Andrés
author_facet Barbosa, Andrés
Balagué, Vanessa
Valera, Francisco
Martínez, Ana
Benzal, Jesús
Motas, Miguel
Diaz, Julia Inés
Mira, Alex
Pedrós Alió, Carlos
author_role author
author2 Balagué, Vanessa
Valera, Francisco
Martínez, Ana
Benzal, Jesús
Motas, Miguel
Diaz, Julia Inés
Mira, Alex
Pedrós Alió, Carlos
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv microbiota
penguin
gastrointestinal tract
pyrsequencing
Diversity
Antarctica
topic microbiota
penguin
gastrointestinal tract
pyrsequencing
Diversity
Antarctica
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The gastrointestinal tract microbiota is known to play very important roles in the well being of animals. It is a complex community composed by hundreds of microbial species interacting closely among them and with their host, that is, a microbial ecosystem. The development of high throughput sequencing techniques allows studying the diversity of such communities in a realistic way and considerable work has been carried out in mammals and some birds such as chickens. Wild birds have received less attention and in particular, in the case of penguins, only a few individuals of five species have been examined with molecular techniques. We collected cloacal samples from Chinstrap penguins in the Vapour Col rookery in Deception Island, Antarctica, and carried out pyrosequencing of the V1-V3 region of the 16S rDNA in samples from 53 individuals, 27 adults and 26 chicks. This provided the first description of the Chinstrap penguin gastrointestinal tract microbiota and the most extensive in any penguin species. Firmicutes, Bacteoridetes, Proteobacteria, Fusobacteria, Actinobacteria, and Tenericutes were the main components. There were large differences between chicks and adults. The former had more Firmicutes and the latter more Bacteroidetes and Proteobacteria. In addition, adults had richer and more diverse bacterial communities than chicks. These differences were also observed between parents and their offspring. On the other hand, nests explained differences in bacterial communities only among chicks. We suggest that environmental factors have a higher importance than genetic factors in the microbiota composition of chicks. The results also showed surprisingly large differences in community composition with other Antarctic penguins including the congeneric Adélie and Gentoo penguins.
Fil: Barbosa, Andrés. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; España
Fil: Balagué, Vanessa. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Fil: Valera, Francisco. Consejo Superior de Investigaciones Científicas. Estación Experimental de Zonas Aridas; España
Fil: Martínez, Ana. Universidad Nacional Autónoma de México; México
Fil: Benzal, Jesús. Consejo Superior de Investigaciones Científicas. Estación Experimental de Zonas Aridas; España
Fil: Motas, Miguel. Universidad de Murcia; España
Fil: Diaz, Julia Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Mira, Alex. Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana; España
Fil: Pedrós Alió, Carlos. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
description The gastrointestinal tract microbiota is known to play very important roles in the well being of animals. It is a complex community composed by hundreds of microbial species interacting closely among them and with their host, that is, a microbial ecosystem. The development of high throughput sequencing techniques allows studying the diversity of such communities in a realistic way and considerable work has been carried out in mammals and some birds such as chickens. Wild birds have received less attention and in particular, in the case of penguins, only a few individuals of five species have been examined with molecular techniques. We collected cloacal samples from Chinstrap penguins in the Vapour Col rookery in Deception Island, Antarctica, and carried out pyrosequencing of the V1-V3 region of the 16S rDNA in samples from 53 individuals, 27 adults and 26 chicks. This provided the first description of the Chinstrap penguin gastrointestinal tract microbiota and the most extensive in any penguin species. Firmicutes, Bacteoridetes, Proteobacteria, Fusobacteria, Actinobacteria, and Tenericutes were the main components. There were large differences between chicks and adults. The former had more Firmicutes and the latter more Bacteroidetes and Proteobacteria. In addition, adults had richer and more diverse bacterial communities than chicks. These differences were also observed between parents and their offspring. On the other hand, nests explained differences in bacterial communities only among chicks. We suggest that environmental factors have a higher importance than genetic factors in the microbiota composition of chicks. The results also showed surprisingly large differences in community composition with other Antarctic penguins including the congeneric Adélie and Gentoo penguins.
publishDate 2016
dc.date.none.fl_str_mv 2016-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48998
Barbosa, Andrés; Balagué, Vanessa; Valera, Francisco; Martínez, Ana; Benzal, Jesús; et al.; Age-related differences in the gastrointestinal microbiota of chinstrap penguins (pygoscelis antarctica); Public Library of Science; Plos One; 11; 4; 4-2016; 1-17; e0153215
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48998
identifier_str_mv Barbosa, Andrés; Balagué, Vanessa; Valera, Francisco; Martínez, Ana; Benzal, Jesús; et al.; Age-related differences in the gastrointestinal microbiota of chinstrap penguins (pygoscelis antarctica); Public Library of Science; Plos One; 11; 4; 4-2016; 1-17; e0153215
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0153215
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0153215
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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