PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by F MRI

Autores
Srinivas, Mangala; Tel, Jurjen; Schreibelt, Gerty; Bonetto, Fernando Jose; Cruz, Luis Javier; Amiri, Houshang; Heerschap, Arend; Figdor, Carl G.; de Vries, I. Jolanda M.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aim: In vivo imaging using 19F MRI is advantageous, due to its ability to quantify cell numbers, but is limited for a lack of suitable labels. Here, we formulate two stable and clinically applicable labels for tracking two populations of primary human dendritic cells (DCs) simultaneously. Materials & methods: Plasmacytoid and myeloid DCs are able to take up sufficient nanoparticles (200 nm) for imaging (1012 19F's per cell), despite being relatively nonphagocytic. Results: Clinically relevant numbers of labeled DCs could be imaged in about 10 min, even on a clinical scanner. Conclusion: We demonstrate the use of perfluorocarbon nanoparticles for simultaneous 19F MRI of distinct cell populations in a clinical setting, without spectroscopic imaging.
Fil: Srinivas, Mangala. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Tel, Jurjen. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Schreibelt, Gerty. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Bonetto, Fernando Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Física del Litoral; Argentina
Fil: Cruz, Luis Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Física del Litoral; Argentina
Fil: Amiri, Houshang. Radboud University Medical Center. Department of Radiology; Países Bajos. Kerman University of Medical Sciences. Institute of Neuropharmacology. Neuroscience Research Center; Irán
Fil: Heerschap, Arend. Radboud University Medical Center. Department of Radiology; Países Bajos
Fil: Figdor, Carl G.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: de Vries, I. Jolanda M.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Materia
Perfluorocarbons
19f Mri
Fluorescence
Cell Therapy
In-Vivo Imaging.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4426
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4426
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by F MRISrinivas, MangalaTel, JurjenSchreibelt, GertyBonetto, Fernando JoseCruz, Luis JavierAmiri, HoushangHeerschap, ArendFigdor, Carl G.de Vries, I. Jolanda M.Perfluorocarbons19f MriFluorescenceCell TherapyIn-Vivo Imaging.https://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Aim: In vivo imaging using 19F MRI is advantageous, due to its ability to quantify cell numbers, but is limited for a lack of suitable labels. Here, we formulate two stable and clinically applicable labels for tracking two populations of primary human dendritic cells (DCs) simultaneously. Materials & methods: Plasmacytoid and myeloid DCs are able to take up sufficient nanoparticles (200 nm) for imaging (1012 19F's per cell), despite being relatively nonphagocytic. Results: Clinically relevant numbers of labeled DCs could be imaged in about 10 min, even on a clinical scanner. Conclusion: We demonstrate the use of perfluorocarbon nanoparticles for simultaneous 19F MRI of distinct cell populations in a clinical setting, without spectroscopic imaging.Fil: Srinivas, Mangala. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosFil: Tel, Jurjen. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosFil: Schreibelt, Gerty. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosFil: Bonetto, Fernando Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Física del Litoral; ArgentinaFil: Cruz, Luis Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Física del Litoral; ArgentinaFil: Amiri, Houshang. Radboud University Medical Center. Department of Radiology; Países Bajos. Kerman University of Medical Sciences. Institute of Neuropharmacology. Neuroscience Research Center; IránFil: Heerschap, Arend. Radboud University Medical Center. Department of Radiology; Países BajosFil: Figdor, Carl G.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosFil: de Vries, I. Jolanda M.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosFuture Medicine2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4426Srinivas, Mangala; Tel, Jurjen; Schreibelt, Gerty; Bonetto, Fernando Jose; Cruz, Luis Javier; et al.; PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by F MRI; Future Medicine; Nanomedicine; 10; 15; 9-2015; 2339-23481743-5889enginfo:eu-repo/semantics/altIdentifier/url/http://www.futuremedicine.com/doi/abs/10.2217/NNM.15.76info:eu-repo/semantics/altIdentifier/doi/doi:10.2217/nnm.15.76info:eu-repo/semantics/altIdentifier/issn/1743-5889info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:33:09Zoai:ri.conicet.gov.ar:11336/4426instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:33:09.327CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by F MRI
title PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by F MRI
spellingShingle PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by F MRI
Srinivas, Mangala
Perfluorocarbons
19f Mri
Fluorescence
Cell Therapy
In-Vivo Imaging.
title_short PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by F MRI
title_full PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by F MRI
title_fullStr PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by F MRI
title_full_unstemmed PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by F MRI
title_sort PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by F MRI
dc.creator.none.fl_str_mv Srinivas, Mangala
Tel, Jurjen
Schreibelt, Gerty
Bonetto, Fernando Jose
Cruz, Luis Javier
Amiri, Houshang
Heerschap, Arend
Figdor, Carl G.
de Vries, I. Jolanda M.
author Srinivas, Mangala
author_facet Srinivas, Mangala
Tel, Jurjen
Schreibelt, Gerty
Bonetto, Fernando Jose
Cruz, Luis Javier
Amiri, Houshang
Heerschap, Arend
Figdor, Carl G.
de Vries, I. Jolanda M.
author_role author
author2 Tel, Jurjen
Schreibelt, Gerty
Bonetto, Fernando Jose
Cruz, Luis Javier
Amiri, Houshang
Heerschap, Arend
Figdor, Carl G.
de Vries, I. Jolanda M.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Perfluorocarbons
19f Mri
Fluorescence
Cell Therapy
In-Vivo Imaging.
topic Perfluorocarbons
19f Mri
Fluorescence
Cell Therapy
In-Vivo Imaging.
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Aim: In vivo imaging using 19F MRI is advantageous, due to its ability to quantify cell numbers, but is limited for a lack of suitable labels. Here, we formulate two stable and clinically applicable labels for tracking two populations of primary human dendritic cells (DCs) simultaneously. Materials & methods: Plasmacytoid and myeloid DCs are able to take up sufficient nanoparticles (200 nm) for imaging (1012 19F's per cell), despite being relatively nonphagocytic. Results: Clinically relevant numbers of labeled DCs could be imaged in about 10 min, even on a clinical scanner. Conclusion: We demonstrate the use of perfluorocarbon nanoparticles for simultaneous 19F MRI of distinct cell populations in a clinical setting, without spectroscopic imaging.
Fil: Srinivas, Mangala. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Tel, Jurjen. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Schreibelt, Gerty. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Bonetto, Fernando Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Física del Litoral; Argentina
Fil: Cruz, Luis Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Física del Litoral; Argentina
Fil: Amiri, Houshang. Radboud University Medical Center. Department of Radiology; Países Bajos. Kerman University of Medical Sciences. Institute of Neuropharmacology. Neuroscience Research Center; Irán
Fil: Heerschap, Arend. Radboud University Medical Center. Department of Radiology; Países Bajos
Fil: Figdor, Carl G.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: de Vries, I. Jolanda M.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
description Aim: In vivo imaging using 19F MRI is advantageous, due to its ability to quantify cell numbers, but is limited for a lack of suitable labels. Here, we formulate two stable and clinically applicable labels for tracking two populations of primary human dendritic cells (DCs) simultaneously. Materials & methods: Plasmacytoid and myeloid DCs are able to take up sufficient nanoparticles (200 nm) for imaging (1012 19F's per cell), despite being relatively nonphagocytic. Results: Clinically relevant numbers of labeled DCs could be imaged in about 10 min, even on a clinical scanner. Conclusion: We demonstrate the use of perfluorocarbon nanoparticles for simultaneous 19F MRI of distinct cell populations in a clinical setting, without spectroscopic imaging.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4426
Srinivas, Mangala; Tel, Jurjen; Schreibelt, Gerty; Bonetto, Fernando Jose; Cruz, Luis Javier; et al.; PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by F MRI; Future Medicine; Nanomedicine; 10; 15; 9-2015; 2339-2348
1743-5889
url http://hdl.handle.net/11336/4426
identifier_str_mv Srinivas, Mangala; Tel, Jurjen; Schreibelt, Gerty; Bonetto, Fernando Jose; Cruz, Luis Javier; et al.; PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by F MRI; Future Medicine; Nanomedicine; 10; 15; 9-2015; 2339-2348
1743-5889
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.futuremedicine.com/doi/abs/10.2217/NNM.15.76
info:eu-repo/semantics/altIdentifier/doi/doi:10.2217/nnm.15.76
info:eu-repo/semantics/altIdentifier/issn/1743-5889
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Future Medicine
publisher.none.fl_str_mv Future Medicine
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781945513508864
score 12.982451

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