Potential zoonotic pathovars of Diarrheagenic Escherichia coli detected in lambs for human consumption from Tierra de Fuego

Autores
Blanco Crivelli, Ximena; Bonino, Maria Paz; Sanin, Mariana Soledad; Petrina, Juan Facundo; Disalvo, Vilma Noelia; Massa, Rosana; Miliwebsky, Elizabeth; Navarro, Armando Jose; Chinen, Isabel; Bentancor, Adriana Beatriz
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Diarrheagenic Escherichia coli (DEC) pathovars impact childhood health. The southern region of Argentina shows the highest incidence of hemolytic uremic syndrome (HUS) in children of the country. The big island of Tierra del Fuego (TDF) in Argentina registered an incidence of five cases/100,000 inhabitants of HUS in 2019. This work aimed to establish the prevalence of STEC, EPEC, and EAEC in lambs slaughtered in abattoirs from TDF as well as to characterize thephenotypes and the genotypes of the isolated pathogens. The prevalence was 26.6% for stx+, 5.7% for eae+, and 0.27% for aagR+/aaiC+. Twelve STEC isolates were obtained and belonged to the following serotypes: O70:HNT, O81:H21, O81:HNT, O102:H6, O128ab:H2, O174:H8, and O174:HNT. Their genotypic profiles were stx1c (2), stx1c/ehxA (3), stx2b/ehxA (1), stx1c/stx2b (2), and stx1c/stx2/ehxA(4). Six EPEC isolates were obtained and corresponded to five serotypes: O2:H40, O32:H8, O56:H6, O108:H21, and O177:H25. All the EPEC isolates were bfpA- and two were ehxA+. By XbaI-PFGE of 17 isolates, two clusters were identified. By antimicrobial susceptibility tests, 8/12 STEC and 5/6 EPEC were resistant to at least one antibiotic. This work provides new data to understand the ecology ofDEC in TDF and confirms that ovine are an important carrier of these pathogens in the region.
Fil: Blanco Crivelli, Ximena. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; Argentina
Fil: Bonino, Maria Paz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sanin, Mariana Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; Argentina
Fil: Petrina, Juan Facundo. Ministerio de Salud de Tierra del Fuego; Argentina
Fil: Disalvo, Vilma Noelia. No especifíca;
Fil: Massa, Rosana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Fil: Miliwebsky, Elizabeth. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Fil: Navarro, Armando Jose. Universidad Nacional Autónoma de México; México
Fil: Chinen, Isabel. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Fil: Bentancor, Adriana Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; Argentina
Materia
DIARRHEAGENIC ESCHERICHIA COLI
LAMB
CARRIER
ARGENTINA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/239129
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Potential zoonotic pathovars of Diarrheagenic Escherichia coli detected in lambs for human consumption from Tierra de FuegoBlanco Crivelli, XimenaBonino, Maria PazSanin, Mariana SoledadPetrina, Juan FacundoDisalvo, Vilma NoeliaMassa, RosanaMiliwebsky, ElizabethNavarro, Armando JoseChinen, IsabelBentancor, Adriana BeatrizDIARRHEAGENIC ESCHERICHIA COLILAMBCARRIERARGENTINAhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Diarrheagenic Escherichia coli (DEC) pathovars impact childhood health. The southern region of Argentina shows the highest incidence of hemolytic uremic syndrome (HUS) in children of the country. The big island of Tierra del Fuego (TDF) in Argentina registered an incidence of five cases/100,000 inhabitants of HUS in 2019. This work aimed to establish the prevalence of STEC, EPEC, and EAEC in lambs slaughtered in abattoirs from TDF as well as to characterize thephenotypes and the genotypes of the isolated pathogens. The prevalence was 26.6% for stx+, 5.7% for eae+, and 0.27% for aagR+/aaiC+. Twelve STEC isolates were obtained and belonged to the following serotypes: O70:HNT, O81:H21, O81:HNT, O102:H6, O128ab:H2, O174:H8, and O174:HNT. Their genotypic profiles were stx1c (2), stx1c/ehxA (3), stx2b/ehxA (1), stx1c/stx2b (2), and stx1c/stx2/ehxA(4). Six EPEC isolates were obtained and corresponded to five serotypes: O2:H40, O32:H8, O56:H6, O108:H21, and O177:H25. All the EPEC isolates were bfpA- and two were ehxA+. By XbaI-PFGE of 17 isolates, two clusters were identified. By antimicrobial susceptibility tests, 8/12 STEC and 5/6 EPEC were resistant to at least one antibiotic. This work provides new data to understand the ecology ofDEC in TDF and confirms that ovine are an important carrier of these pathogens in the region.Fil: Blanco Crivelli, Ximena. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; ArgentinaFil: Bonino, Maria Paz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sanin, Mariana Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; ArgentinaFil: Petrina, Juan Facundo. Ministerio de Salud de Tierra del Fuego; ArgentinaFil: Disalvo, Vilma Noelia. No especifíca;Fil: Massa, Rosana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; ArgentinaFil: Miliwebsky, Elizabeth. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; ArgentinaFil: Navarro, Armando Jose. Universidad Nacional Autónoma de México; MéxicoFil: Chinen, Isabel. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; ArgentinaFil: Bentancor, Adriana Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; ArgentinaMDPI2021-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/239129Blanco Crivelli, Ximena; Bonino, Maria Paz; Sanin, Mariana Soledad; Petrina, Juan Facundo; Disalvo, Vilma Noelia; et al.; Potential zoonotic pathovars of Diarrheagenic Escherichia coli detected in lambs for human consumption from Tierra de Fuego; MDPI; Microorganisms; 8-2021; 1-142076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms9081710info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/9/8/1710#citeinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:42:01Zoai:ri.conicet.gov.ar:11336/239129instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:42:01.764CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Potential zoonotic pathovars of Diarrheagenic Escherichia coli detected in lambs for human consumption from Tierra de Fuego
title Potential zoonotic pathovars of Diarrheagenic Escherichia coli detected in lambs for human consumption from Tierra de Fuego
spellingShingle Potential zoonotic pathovars of Diarrheagenic Escherichia coli detected in lambs for human consumption from Tierra de Fuego
Blanco Crivelli, Ximena
DIARRHEAGENIC ESCHERICHIA COLI
LAMB
CARRIER
ARGENTINA
title_short Potential zoonotic pathovars of Diarrheagenic Escherichia coli detected in lambs for human consumption from Tierra de Fuego
title_full Potential zoonotic pathovars of Diarrheagenic Escherichia coli detected in lambs for human consumption from Tierra de Fuego
title_fullStr Potential zoonotic pathovars of Diarrheagenic Escherichia coli detected in lambs for human consumption from Tierra de Fuego
title_full_unstemmed Potential zoonotic pathovars of Diarrheagenic Escherichia coli detected in lambs for human consumption from Tierra de Fuego
title_sort Potential zoonotic pathovars of Diarrheagenic Escherichia coli detected in lambs for human consumption from Tierra de Fuego
dc.creator.none.fl_str_mv Blanco Crivelli, Ximena
Bonino, Maria Paz
Sanin, Mariana Soledad
Petrina, Juan Facundo
Disalvo, Vilma Noelia
Massa, Rosana
Miliwebsky, Elizabeth
Navarro, Armando Jose
Chinen, Isabel
Bentancor, Adriana Beatriz
author Blanco Crivelli, Ximena
author_facet Blanco Crivelli, Ximena
Bonino, Maria Paz
Sanin, Mariana Soledad
Petrina, Juan Facundo
Disalvo, Vilma Noelia
Massa, Rosana
Miliwebsky, Elizabeth
Navarro, Armando Jose
Chinen, Isabel
Bentancor, Adriana Beatriz
author_role author
author2 Bonino, Maria Paz
Sanin, Mariana Soledad
Petrina, Juan Facundo
Disalvo, Vilma Noelia
Massa, Rosana
Miliwebsky, Elizabeth
Navarro, Armando Jose
Chinen, Isabel
Bentancor, Adriana Beatriz
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DIARRHEAGENIC ESCHERICHIA COLI
LAMB
CARRIER
ARGENTINA
topic DIARRHEAGENIC ESCHERICHIA COLI
LAMB
CARRIER
ARGENTINA
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Diarrheagenic Escherichia coli (DEC) pathovars impact childhood health. The southern region of Argentina shows the highest incidence of hemolytic uremic syndrome (HUS) in children of the country. The big island of Tierra del Fuego (TDF) in Argentina registered an incidence of five cases/100,000 inhabitants of HUS in 2019. This work aimed to establish the prevalence of STEC, EPEC, and EAEC in lambs slaughtered in abattoirs from TDF as well as to characterize thephenotypes and the genotypes of the isolated pathogens. The prevalence was 26.6% for stx+, 5.7% for eae+, and 0.27% for aagR+/aaiC+. Twelve STEC isolates were obtained and belonged to the following serotypes: O70:HNT, O81:H21, O81:HNT, O102:H6, O128ab:H2, O174:H8, and O174:HNT. Their genotypic profiles were stx1c (2), stx1c/ehxA (3), stx2b/ehxA (1), stx1c/stx2b (2), and stx1c/stx2/ehxA(4). Six EPEC isolates were obtained and corresponded to five serotypes: O2:H40, O32:H8, O56:H6, O108:H21, and O177:H25. All the EPEC isolates were bfpA- and two were ehxA+. By XbaI-PFGE of 17 isolates, two clusters were identified. By antimicrobial susceptibility tests, 8/12 STEC and 5/6 EPEC were resistant to at least one antibiotic. This work provides new data to understand the ecology ofDEC in TDF and confirms that ovine are an important carrier of these pathogens in the region.
Fil: Blanco Crivelli, Ximena. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; Argentina
Fil: Bonino, Maria Paz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sanin, Mariana Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; Argentina
Fil: Petrina, Juan Facundo. Ministerio de Salud de Tierra del Fuego; Argentina
Fil: Disalvo, Vilma Noelia. No especifíca;
Fil: Massa, Rosana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Fil: Miliwebsky, Elizabeth. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Fil: Navarro, Armando Jose. Universidad Nacional Autónoma de México; México
Fil: Chinen, Isabel. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Fil: Bentancor, Adriana Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Microbiología Veterinaria; Argentina
description Diarrheagenic Escherichia coli (DEC) pathovars impact childhood health. The southern region of Argentina shows the highest incidence of hemolytic uremic syndrome (HUS) in children of the country. The big island of Tierra del Fuego (TDF) in Argentina registered an incidence of five cases/100,000 inhabitants of HUS in 2019. This work aimed to establish the prevalence of STEC, EPEC, and EAEC in lambs slaughtered in abattoirs from TDF as well as to characterize thephenotypes and the genotypes of the isolated pathogens. The prevalence was 26.6% for stx+, 5.7% for eae+, and 0.27% for aagR+/aaiC+. Twelve STEC isolates were obtained and belonged to the following serotypes: O70:HNT, O81:H21, O81:HNT, O102:H6, O128ab:H2, O174:H8, and O174:HNT. Their genotypic profiles were stx1c (2), stx1c/ehxA (3), stx2b/ehxA (1), stx1c/stx2b (2), and stx1c/stx2/ehxA(4). Six EPEC isolates were obtained and corresponded to five serotypes: O2:H40, O32:H8, O56:H6, O108:H21, and O177:H25. All the EPEC isolates were bfpA- and two were ehxA+. By XbaI-PFGE of 17 isolates, two clusters were identified. By antimicrobial susceptibility tests, 8/12 STEC and 5/6 EPEC were resistant to at least one antibiotic. This work provides new data to understand the ecology ofDEC in TDF and confirms that ovine are an important carrier of these pathogens in the region.
publishDate 2021
dc.date.none.fl_str_mv 2021-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/239129
Blanco Crivelli, Ximena; Bonino, Maria Paz; Sanin, Mariana Soledad; Petrina, Juan Facundo; Disalvo, Vilma Noelia; et al.; Potential zoonotic pathovars of Diarrheagenic Escherichia coli detected in lambs for human consumption from Tierra de Fuego; MDPI; Microorganisms; 8-2021; 1-14
2076-2607
CONICET Digital
CONICET
url http://hdl.handle.net/11336/239129
identifier_str_mv Blanco Crivelli, Ximena; Bonino, Maria Paz; Sanin, Mariana Soledad; Petrina, Juan Facundo; Disalvo, Vilma Noelia; et al.; Potential zoonotic pathovars of Diarrheagenic Escherichia coli detected in lambs for human consumption from Tierra de Fuego; MDPI; Microorganisms; 8-2021; 1-14
2076-2607
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/9/8/1710#cite
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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