Prenatal testosterone excess alters Sertoli and germ cell number and testicular FSH receptor expression in rams

Autores
Rojas García, Pedro P.; Recabarren, Mónica; Sarabia, Luis; Schön, Jennifer; Gabler, Christoph; Einspanier, Ralf; Maliqueo, Manuel; Sir Petermann, Teresa; Rey, Rodolfo Alberto; Recabarren, Sergio E.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Exposure to excess testosterone (T) during fetal life has a profound impact on the metabolic and reproductive functions in the female's postnatal life. However, less is known about the effects of excess testosterone in males. The aim of the present study was to evaluate the impact (consequences) of an excess of T during fetal development on mature male testis. The testicular evaluation was by histological analysis and by determination of mRNA expression of the FSH receptor (FSH-R), transforming growth factor-β type I receptor (TβR-I), and two members of the TGF-β superfamily, transforming growth factor-β3 (TGFβ3) and anti-Müllerian hormone (AMH) in males born to mothers receiving an excess of T during pregnancy. At 42 wk of age, postpubertal males born to mothers treated with 30 mg of T propionate twice weekly from day 30 to 90, followed by 40 mg of T propionate from day 90 to 120 of pregnancy (T males), showed higher concentrations of FSH in response to a GnRH analog, a higher number of Sertoli cells/seminiferous tubule cross-section, and a lower number of germ cells/tubules (P < 0.05) than control males (C males) born to mothers treated with the vehicle. The mRNA expression of FSH-R and of TβR-I was higher in T males compared with C males (P < 0.05). Moreover, in T males, AMH expression level correlated negatively with the expression level of TGFβ3. In C males, this latter correlation was not observed. These results suggest that prenatal exposure to an excess of T can negatively modify some histological and molecular characteristics of the mature testis.
Fil: Rojas García, Pedro P.. Universidad de Concepción; Chile
Fil: Recabarren, Mónica. Universidad de Concepción; Chile
Fil: Sarabia, Luis. Universidad de Chile; Chile
Fil: Schön, Jennifer. Freie Universität; Alemania
Fil: Gabler, Christoph. Freie Universität; Alemania
Fil: Einspanier, Ralf. Freie Universität; Alemania
Fil: Maliqueo, Manuel. Universidad de Chile; Chile
Fil: Sir Petermann, Teresa. Universidad de Chile; Chile
Fil: Rey, Rodolfo Alberto. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
Fil: Recabarren, Sergio E.. Universidad de Concepción; Chile
Materia
FETAL PROGRAMMING
FOLLICLE-STIMULATING HORMONE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/98631
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Prenatal testosterone excess alters Sertoli and germ cell number and testicular FSH receptor expression in ramsRojas García, Pedro P.Recabarren, MónicaSarabia, LuisSchön, JenniferGabler, ChristophEinspanier, RalfMaliqueo, ManuelSir Petermann, TeresaRey, Rodolfo AlbertoRecabarren, Sergio E.FETAL PROGRAMMINGFOLLICLE-STIMULATING HORMONEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Exposure to excess testosterone (T) during fetal life has a profound impact on the metabolic and reproductive functions in the female's postnatal life. However, less is known about the effects of excess testosterone in males. The aim of the present study was to evaluate the impact (consequences) of an excess of T during fetal development on mature male testis. The testicular evaluation was by histological analysis and by determination of mRNA expression of the FSH receptor (FSH-R), transforming growth factor-β type I receptor (TβR-I), and two members of the TGF-β superfamily, transforming growth factor-β3 (TGFβ3) and anti-Müllerian hormone (AMH) in males born to mothers receiving an excess of T during pregnancy. At 42 wk of age, postpubertal males born to mothers treated with 30 mg of T propionate twice weekly from day 30 to 90, followed by 40 mg of T propionate from day 90 to 120 of pregnancy (T males), showed higher concentrations of FSH in response to a GnRH analog, a higher number of Sertoli cells/seminiferous tubule cross-section, and a lower number of germ cells/tubules (P < 0.05) than control males (C males) born to mothers treated with the vehicle. The mRNA expression of FSH-R and of TβR-I was higher in T males compared with C males (P < 0.05). Moreover, in T males, AMH expression level correlated negatively with the expression level of TGFβ3. In C males, this latter correlation was not observed. These results suggest that prenatal exposure to an excess of T can negatively modify some histological and molecular characteristics of the mature testis.Fil: Rojas García, Pedro P.. Universidad de Concepción; ChileFil: Recabarren, Mónica. Universidad de Concepción; ChileFil: Sarabia, Luis. Universidad de Chile; ChileFil: Schön, Jennifer. Freie Universität; AlemaniaFil: Gabler, Christoph. Freie Universität; AlemaniaFil: Einspanier, Ralf. Freie Universität; AlemaniaFil: Maliqueo, Manuel. Universidad de Chile; ChileFil: Sir Petermann, Teresa. Universidad de Chile; ChileFil: Rey, Rodolfo Alberto. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; ArgentinaFil: Recabarren, Sergio E.. Universidad de Concepción; ChileAmerican Physiological Society2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/98631Rojas García, Pedro P.; Recabarren, Mónica; Sarabia, Luis; Schön, Jennifer; Gabler, Christoph; et al.; Prenatal testosterone excess alters Sertoli and germ cell number and testicular FSH receptor expression in rams; American Physiological Society; American Journal Of Physiology-endocrinology And Metabolism; 299; 6; 12-2010; 1-90193-1849CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1152/ajpendo.00032.2010info:eu-repo/semantics/altIdentifier/url/https://journals.physiology.org/doi/full/10.1152/ajpendo.00032.2010#ref-list-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:10Zoai:ri.conicet.gov.ar:11336/98631instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:10.706CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Prenatal testosterone excess alters Sertoli and germ cell number and testicular FSH receptor expression in rams
title Prenatal testosterone excess alters Sertoli and germ cell number and testicular FSH receptor expression in rams
spellingShingle Prenatal testosterone excess alters Sertoli and germ cell number and testicular FSH receptor expression in rams
Rojas García, Pedro P.
FETAL PROGRAMMING
FOLLICLE-STIMULATING HORMONE
title_short Prenatal testosterone excess alters Sertoli and germ cell number and testicular FSH receptor expression in rams
title_full Prenatal testosterone excess alters Sertoli and germ cell number and testicular FSH receptor expression in rams
title_fullStr Prenatal testosterone excess alters Sertoli and germ cell number and testicular FSH receptor expression in rams
title_full_unstemmed Prenatal testosterone excess alters Sertoli and germ cell number and testicular FSH receptor expression in rams
title_sort Prenatal testosterone excess alters Sertoli and germ cell number and testicular FSH receptor expression in rams
dc.creator.none.fl_str_mv Rojas García, Pedro P.
Recabarren, Mónica
Sarabia, Luis
Schön, Jennifer
Gabler, Christoph
Einspanier, Ralf
Maliqueo, Manuel
Sir Petermann, Teresa
Rey, Rodolfo Alberto
Recabarren, Sergio E.
author Rojas García, Pedro P.
author_facet Rojas García, Pedro P.
Recabarren, Mónica
Sarabia, Luis
Schön, Jennifer
Gabler, Christoph
Einspanier, Ralf
Maliqueo, Manuel
Sir Petermann, Teresa
Rey, Rodolfo Alberto
Recabarren, Sergio E.
author_role author
author2 Recabarren, Mónica
Sarabia, Luis
Schön, Jennifer
Gabler, Christoph
Einspanier, Ralf
Maliqueo, Manuel
Sir Petermann, Teresa
Rey, Rodolfo Alberto
Recabarren, Sergio E.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FETAL PROGRAMMING
FOLLICLE-STIMULATING HORMONE
topic FETAL PROGRAMMING
FOLLICLE-STIMULATING HORMONE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Exposure to excess testosterone (T) during fetal life has a profound impact on the metabolic and reproductive functions in the female's postnatal life. However, less is known about the effects of excess testosterone in males. The aim of the present study was to evaluate the impact (consequences) of an excess of T during fetal development on mature male testis. The testicular evaluation was by histological analysis and by determination of mRNA expression of the FSH receptor (FSH-R), transforming growth factor-β type I receptor (TβR-I), and two members of the TGF-β superfamily, transforming growth factor-β3 (TGFβ3) and anti-Müllerian hormone (AMH) in males born to mothers receiving an excess of T during pregnancy. At 42 wk of age, postpubertal males born to mothers treated with 30 mg of T propionate twice weekly from day 30 to 90, followed by 40 mg of T propionate from day 90 to 120 of pregnancy (T males), showed higher concentrations of FSH in response to a GnRH analog, a higher number of Sertoli cells/seminiferous tubule cross-section, and a lower number of germ cells/tubules (P < 0.05) than control males (C males) born to mothers treated with the vehicle. The mRNA expression of FSH-R and of TβR-I was higher in T males compared with C males (P < 0.05). Moreover, in T males, AMH expression level correlated negatively with the expression level of TGFβ3. In C males, this latter correlation was not observed. These results suggest that prenatal exposure to an excess of T can negatively modify some histological and molecular characteristics of the mature testis.
Fil: Rojas García, Pedro P.. Universidad de Concepción; Chile
Fil: Recabarren, Mónica. Universidad de Concepción; Chile
Fil: Sarabia, Luis. Universidad de Chile; Chile
Fil: Schön, Jennifer. Freie Universität; Alemania
Fil: Gabler, Christoph. Freie Universität; Alemania
Fil: Einspanier, Ralf. Freie Universität; Alemania
Fil: Maliqueo, Manuel. Universidad de Chile; Chile
Fil: Sir Petermann, Teresa. Universidad de Chile; Chile
Fil: Rey, Rodolfo Alberto. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
Fil: Recabarren, Sergio E.. Universidad de Concepción; Chile
description Exposure to excess testosterone (T) during fetal life has a profound impact on the metabolic and reproductive functions in the female's postnatal life. However, less is known about the effects of excess testosterone in males. The aim of the present study was to evaluate the impact (consequences) of an excess of T during fetal development on mature male testis. The testicular evaluation was by histological analysis and by determination of mRNA expression of the FSH receptor (FSH-R), transforming growth factor-β type I receptor (TβR-I), and two members of the TGF-β superfamily, transforming growth factor-β3 (TGFβ3) and anti-Müllerian hormone (AMH) in males born to mothers receiving an excess of T during pregnancy. At 42 wk of age, postpubertal males born to mothers treated with 30 mg of T propionate twice weekly from day 30 to 90, followed by 40 mg of T propionate from day 90 to 120 of pregnancy (T males), showed higher concentrations of FSH in response to a GnRH analog, a higher number of Sertoli cells/seminiferous tubule cross-section, and a lower number of germ cells/tubules (P < 0.05) than control males (C males) born to mothers treated with the vehicle. The mRNA expression of FSH-R and of TβR-I was higher in T males compared with C males (P < 0.05). Moreover, in T males, AMH expression level correlated negatively with the expression level of TGFβ3. In C males, this latter correlation was not observed. These results suggest that prenatal exposure to an excess of T can negatively modify some histological and molecular characteristics of the mature testis.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/98631
Rojas García, Pedro P.; Recabarren, Mónica; Sarabia, Luis; Schön, Jennifer; Gabler, Christoph; et al.; Prenatal testosterone excess alters Sertoli and germ cell number and testicular FSH receptor expression in rams; American Physiological Society; American Journal Of Physiology-endocrinology And Metabolism; 299; 6; 12-2010; 1-9
0193-1849
CONICET Digital
CONICET
url http://hdl.handle.net/11336/98631
identifier_str_mv Rojas García, Pedro P.; Recabarren, Mónica; Sarabia, Luis; Schön, Jennifer; Gabler, Christoph; et al.; Prenatal testosterone excess alters Sertoli and germ cell number and testicular FSH receptor expression in rams; American Physiological Society; American Journal Of Physiology-endocrinology And Metabolism; 299; 6; 12-2010; 1-9
0193-1849
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1152/ajpendo.00032.2010
info:eu-repo/semantics/altIdentifier/url/https://journals.physiology.org/doi/full/10.1152/ajpendo.00032.2010#ref-list-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Physiological Society
publisher.none.fl_str_mv American Physiological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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