Felis catus as a model to study follicle biology in vitro

Autores
Rojo, Julieta Laura; Linari, Martina; Musse, Mariana Paula; Peluffo, Marina Cinthia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Purpose The current study was designed to evaluate the responseof individual intact antral follicles from adult femaledomestic cats to a luteinizing hormone (LH) stimulus in vitroby assessing cumulus-oocyte expansion (C-OE) and steroidproduction.Methods C-OE and steroid levels (estradiol [E2] and progesterone[P4]) obtained from individual antral feline follicles(n=366 follicles; n=56 cats) were analyzed after 12 or 24 hof culture in the presence or absence of LH (low [3.4 ng/ml] orhigh [100 ng/ml]).Results At the end of the culture, the highest percentage ofexpanded cumulus-oocyte complexes (COCs) was observedin the LH groups at 12 or 24 h in comparison to their controls(p<0.001). There was a significant increase in expandedCOCs when comparing LH concentrations (high vs. low) at12 or 24 h. Higher levels of both E2 and P4 were observed inthe media from antral follicles after 12 and 24 h of culture inthe presence of LH (both concentration, p<0.05). There wasno association between hormone levels and follicle diameter;high variability was observed in the steroid levels produced byantral follicles within all treatment groups.Conclusions These data indicate, for the first time, that felineantral follicles (0.5-2 mm) from different stages of the naturalestrous cycle can be cultured and will respond to an LH stimulus,based on an increase in steroid levels as well as C-OEafter 12 or 24 h in culture.
Fil: Rojo, Julieta Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Linari, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Musse, Mariana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Peluffo, Marina Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Materia
Antral Follicles
Felis Catus
Steroids
Follicle Culture
Cumulus-Oocyte Expansion
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8128
Acceder
id CONICETDig_56a6458d764416bd811f4e64bf0fa07b
oai_identifier_str oai:ri.conicet.gov.ar:11336/8128
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Felis catus as a model to study follicle biology in vitroRojo, Julieta LauraLinari, MartinaMusse, Mariana PaulaPeluffo, Marina CinthiaAntral FolliclesFelis CatusSteroidsFollicle CultureCumulus-Oocyte Expansionhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Purpose The current study was designed to evaluate the responseof individual intact antral follicles from adult femaledomestic cats to a luteinizing hormone (LH) stimulus in vitroby assessing cumulus-oocyte expansion (C-OE) and steroidproduction.Methods C-OE and steroid levels (estradiol [E2] and progesterone[P4]) obtained from individual antral feline follicles(n=366 follicles; n=56 cats) were analyzed after 12 or 24 hof culture in the presence or absence of LH (low [3.4 ng/ml] orhigh [100 ng/ml]).Results At the end of the culture, the highest percentage ofexpanded cumulus-oocyte complexes (COCs) was observedin the LH groups at 12 or 24 h in comparison to their controls(p<0.001). There was a significant increase in expandedCOCs when comparing LH concentrations (high vs. low) at12 or 24 h. Higher levels of both E2 and P4 were observed inthe media from antral follicles after 12 and 24 h of culture inthe presence of LH (both concentration, p<0.05). There wasno association between hormone levels and follicle diameter;high variability was observed in the steroid levels produced byantral follicles within all treatment groups.Conclusions These data indicate, for the first time, that felineantral follicles (0.5-2 mm) from different stages of the naturalestrous cycle can be cultured and will respond to an LH stimulus,based on an increase in steroid levels as well as C-OEafter 12 or 24 h in culture.Fil: Rojo, Julieta Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Linari, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Musse, Mariana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Peluffo, Marina Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaSpringer/plenum Publishers2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8128Rojo, Julieta Laura; Linari, Martina; Musse, Mariana Paula; Peluffo, Marina Cinthia; Felis catus as a model to study follicle biology in vitro; Springer/plenum Publishers; Journal Of Assisted Reproduction And Genetics; 32; 7; 7-2015; 1105-11111058-0468enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs10815-015-0511-5info:eu-repo/semantics/altIdentifier/doi/10.1007/s10815-015-0511-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:22Zoai:ri.conicet.gov.ar:11336/8128instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:23.157CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Felis catus as a model to study follicle biology in vitro
title Felis catus as a model to study follicle biology in vitro
spellingShingle Felis catus as a model to study follicle biology in vitro
Rojo, Julieta Laura
Antral Follicles
Felis Catus
Steroids
Follicle Culture
Cumulus-Oocyte Expansion
title_short Felis catus as a model to study follicle biology in vitro
title_full Felis catus as a model to study follicle biology in vitro
title_fullStr Felis catus as a model to study follicle biology in vitro
title_full_unstemmed Felis catus as a model to study follicle biology in vitro
title_sort Felis catus as a model to study follicle biology in vitro
dc.creator.none.fl_str_mv Rojo, Julieta Laura
Linari, Martina
Musse, Mariana Paula
Peluffo, Marina Cinthia
author Rojo, Julieta Laura
author_facet Rojo, Julieta Laura
Linari, Martina
Musse, Mariana Paula
Peluffo, Marina Cinthia
author_role author
author2 Linari, Martina
Musse, Mariana Paula
Peluffo, Marina Cinthia
author2_role author
author
author
dc.subject.none.fl_str_mv Antral Follicles
Felis Catus
Steroids
Follicle Culture
Cumulus-Oocyte Expansion
topic Antral Follicles
Felis Catus
Steroids
Follicle Culture
Cumulus-Oocyte Expansion
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Purpose The current study was designed to evaluate the responseof individual intact antral follicles from adult femaledomestic cats to a luteinizing hormone (LH) stimulus in vitroby assessing cumulus-oocyte expansion (C-OE) and steroidproduction.Methods C-OE and steroid levels (estradiol [E2] and progesterone[P4]) obtained from individual antral feline follicles(n=366 follicles; n=56 cats) were analyzed after 12 or 24 hof culture in the presence or absence of LH (low [3.4 ng/ml] orhigh [100 ng/ml]).Results At the end of the culture, the highest percentage ofexpanded cumulus-oocyte complexes (COCs) was observedin the LH groups at 12 or 24 h in comparison to their controls(p<0.001). There was a significant increase in expandedCOCs when comparing LH concentrations (high vs. low) at12 or 24 h. Higher levels of both E2 and P4 were observed inthe media from antral follicles after 12 and 24 h of culture inthe presence of LH (both concentration, p<0.05). There wasno association between hormone levels and follicle diameter;high variability was observed in the steroid levels produced byantral follicles within all treatment groups.Conclusions These data indicate, for the first time, that felineantral follicles (0.5-2 mm) from different stages of the naturalestrous cycle can be cultured and will respond to an LH stimulus,based on an increase in steroid levels as well as C-OEafter 12 or 24 h in culture.
Fil: Rojo, Julieta Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Linari, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Musse, Mariana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Peluffo, Marina Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
description Purpose The current study was designed to evaluate the responseof individual intact antral follicles from adult femaledomestic cats to a luteinizing hormone (LH) stimulus in vitroby assessing cumulus-oocyte expansion (C-OE) and steroidproduction.Methods C-OE and steroid levels (estradiol [E2] and progesterone[P4]) obtained from individual antral feline follicles(n=366 follicles; n=56 cats) were analyzed after 12 or 24 hof culture in the presence or absence of LH (low [3.4 ng/ml] orhigh [100 ng/ml]).Results At the end of the culture, the highest percentage ofexpanded cumulus-oocyte complexes (COCs) was observedin the LH groups at 12 or 24 h in comparison to their controls(p<0.001). There was a significant increase in expandedCOCs when comparing LH concentrations (high vs. low) at12 or 24 h. Higher levels of both E2 and P4 were observed inthe media from antral follicles after 12 and 24 h of culture inthe presence of LH (both concentration, p<0.05). There wasno association between hormone levels and follicle diameter;high variability was observed in the steroid levels produced byantral follicles within all treatment groups.Conclusions These data indicate, for the first time, that felineantral follicles (0.5-2 mm) from different stages of the naturalestrous cycle can be cultured and will respond to an LH stimulus,based on an increase in steroid levels as well as C-OEafter 12 or 24 h in culture.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8128
Rojo, Julieta Laura; Linari, Martina; Musse, Mariana Paula; Peluffo, Marina Cinthia; Felis catus as a model to study follicle biology in vitro; Springer/plenum Publishers; Journal Of Assisted Reproduction And Genetics; 32; 7; 7-2015; 1105-1111
1058-0468
url http://hdl.handle.net/11336/8128
identifier_str_mv Rojo, Julieta Laura; Linari, Martina; Musse, Mariana Paula; Peluffo, Marina Cinthia; Felis catus as a model to study follicle biology in vitro; Springer/plenum Publishers; Journal Of Assisted Reproduction And Genetics; 32; 7; 7-2015; 1105-1111
1058-0468
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs10815-015-0511-5
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10815-015-0511-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer/plenum Publishers
publisher.none.fl_str_mv Springer/plenum Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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