Interaction of dopamine D1 with NMDA NR1 receptors in rat prefrontal cortex

Autores
Kruse, Maria Sol; Prémont, Joel; Krebs, Marie Odile; Jay, Therese M.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Despite the tremendous importance of D1 and NMDA receptors to cognition (working memory, executive functions) and synaptic plasticity in the prefrontal cortex (PFC), little is known about the molecular mechanisms underlying D1-NMDA receptors interactions in this brain area. Here, we show that D1 receptors and the NMDA receptor co-localize in single pyramidal neurons and interneurons in adult rat PFC. NR1 and NR2A expression are found in different neuronal types. Conversely, D1 receptors are predominantly localized in pyramidal-like cells and parvalbumin positive cells. NR1 co-immunoprecipitates with D1 receptor in adult medial PFC. In prefrontal primary cultures, NMDA does not affect the D1 receptor dependent-cAMP production. In contrast, activation of D1 receptor potentiates the NMDA mediated increase in cytosolic Ca2+, an effect that was blocked by a PKA inhibitor. We conclude that D1 receptor potentiates the NMDA-Ca2+ signal by a PKA-dependent mechanism.
Fil: Kruse, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Inserm; Francia. Universite de Paris V; Francia
Fil: Prémont, Joel. Inserm; Francia. Universite de Paris V; Francia
Fil: Krebs, Marie Odile. Inserm; Francia. Universite de Paris V; Francia
Fil: Jay, Therese M.. Inserm; Francia. Universite de Paris V; Francia
Materia
Dopamine D1 Receptor
Nmda
Prefrontal Cortex
Calcium
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24458
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24458
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Interaction of dopamine D1 with NMDA NR1 receptors in rat prefrontal cortexKruse, Maria SolPrémont, JoelKrebs, Marie OdileJay, Therese M.Dopamine D1 ReceptorNmdaPrefrontal CortexCalciumhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Despite the tremendous importance of D1 and NMDA receptors to cognition (working memory, executive functions) and synaptic plasticity in the prefrontal cortex (PFC), little is known about the molecular mechanisms underlying D1-NMDA receptors interactions in this brain area. Here, we show that D1 receptors and the NMDA receptor co-localize in single pyramidal neurons and interneurons in adult rat PFC. NR1 and NR2A expression are found in different neuronal types. Conversely, D1 receptors are predominantly localized in pyramidal-like cells and parvalbumin positive cells. NR1 co-immunoprecipitates with D1 receptor in adult medial PFC. In prefrontal primary cultures, NMDA does not affect the D1 receptor dependent-cAMP production. In contrast, activation of D1 receptor potentiates the NMDA mediated increase in cytosolic Ca2+, an effect that was blocked by a PKA inhibitor. We conclude that D1 receptor potentiates the NMDA-Ca2+ signal by a PKA-dependent mechanism.Fil: Kruse, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Inserm; Francia. Universite de Paris V; FranciaFil: Prémont, Joel. Inserm; Francia. Universite de Paris V; FranciaFil: Krebs, Marie Odile. Inserm; Francia. Universite de Paris V; FranciaFil: Jay, Therese M.. Inserm; Francia. Universite de Paris V; FranciaElsevier2009-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24458Kruse, Maria Sol; Prémont, Joel; Krebs, Marie Odile; Jay, Therese M.; Interaction of dopamine D1 with NMDA NR1 receptors in rat prefrontal cortex; Elsevier; European Neuropsychofarmacology; 19; 4; 1-2009; 296-3040924-977X1873-7862CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.europeanneuropsychopharmacology.com/article/S0924-977X(08)00313-1/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.euroneuro.2008.12.006info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0924977X08003131info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:15:36Zoai:ri.conicet.gov.ar:11336/24458instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:15:36.52CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interaction of dopamine D1 with NMDA NR1 receptors in rat prefrontal cortex
title Interaction of dopamine D1 with NMDA NR1 receptors in rat prefrontal cortex
spellingShingle Interaction of dopamine D1 with NMDA NR1 receptors in rat prefrontal cortex
Kruse, Maria Sol
Dopamine D1 Receptor
Nmda
Prefrontal Cortex
Calcium
title_short Interaction of dopamine D1 with NMDA NR1 receptors in rat prefrontal cortex
title_full Interaction of dopamine D1 with NMDA NR1 receptors in rat prefrontal cortex
title_fullStr Interaction of dopamine D1 with NMDA NR1 receptors in rat prefrontal cortex
title_full_unstemmed Interaction of dopamine D1 with NMDA NR1 receptors in rat prefrontal cortex
title_sort Interaction of dopamine D1 with NMDA NR1 receptors in rat prefrontal cortex
dc.creator.none.fl_str_mv Kruse, Maria Sol
Prémont, Joel
Krebs, Marie Odile
Jay, Therese M.
author Kruse, Maria Sol
author_facet Kruse, Maria Sol
Prémont, Joel
Krebs, Marie Odile
Jay, Therese M.
author_role author
author2 Prémont, Joel
Krebs, Marie Odile
Jay, Therese M.
author2_role author
author
author
dc.subject.none.fl_str_mv Dopamine D1 Receptor
Nmda
Prefrontal Cortex
Calcium
topic Dopamine D1 Receptor
Nmda
Prefrontal Cortex
Calcium
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Despite the tremendous importance of D1 and NMDA receptors to cognition (working memory, executive functions) and synaptic plasticity in the prefrontal cortex (PFC), little is known about the molecular mechanisms underlying D1-NMDA receptors interactions in this brain area. Here, we show that D1 receptors and the NMDA receptor co-localize in single pyramidal neurons and interneurons in adult rat PFC. NR1 and NR2A expression are found in different neuronal types. Conversely, D1 receptors are predominantly localized in pyramidal-like cells and parvalbumin positive cells. NR1 co-immunoprecipitates with D1 receptor in adult medial PFC. In prefrontal primary cultures, NMDA does not affect the D1 receptor dependent-cAMP production. In contrast, activation of D1 receptor potentiates the NMDA mediated increase in cytosolic Ca2+, an effect that was blocked by a PKA inhibitor. We conclude that D1 receptor potentiates the NMDA-Ca2+ signal by a PKA-dependent mechanism.
Fil: Kruse, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Inserm; Francia. Universite de Paris V; Francia
Fil: Prémont, Joel. Inserm; Francia. Universite de Paris V; Francia
Fil: Krebs, Marie Odile. Inserm; Francia. Universite de Paris V; Francia
Fil: Jay, Therese M.. Inserm; Francia. Universite de Paris V; Francia
description Despite the tremendous importance of D1 and NMDA receptors to cognition (working memory, executive functions) and synaptic plasticity in the prefrontal cortex (PFC), little is known about the molecular mechanisms underlying D1-NMDA receptors interactions in this brain area. Here, we show that D1 receptors and the NMDA receptor co-localize in single pyramidal neurons and interneurons in adult rat PFC. NR1 and NR2A expression are found in different neuronal types. Conversely, D1 receptors are predominantly localized in pyramidal-like cells and parvalbumin positive cells. NR1 co-immunoprecipitates with D1 receptor in adult medial PFC. In prefrontal primary cultures, NMDA does not affect the D1 receptor dependent-cAMP production. In contrast, activation of D1 receptor potentiates the NMDA mediated increase in cytosolic Ca2+, an effect that was blocked by a PKA inhibitor. We conclude that D1 receptor potentiates the NMDA-Ca2+ signal by a PKA-dependent mechanism.
publishDate 2009
dc.date.none.fl_str_mv 2009-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24458
Kruse, Maria Sol; Prémont, Joel; Krebs, Marie Odile; Jay, Therese M.; Interaction of dopamine D1 with NMDA NR1 receptors in rat prefrontal cortex; Elsevier; European Neuropsychofarmacology; 19; 4; 1-2009; 296-304
0924-977X
1873-7862
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24458
identifier_str_mv Kruse, Maria Sol; Prémont, Joel; Krebs, Marie Odile; Jay, Therese M.; Interaction of dopamine D1 with NMDA NR1 receptors in rat prefrontal cortex; Elsevier; European Neuropsychofarmacology; 19; 4; 1-2009; 296-304
0924-977X
1873-7862
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.europeanneuropsychopharmacology.com/article/S0924-977X(08)00313-1/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.euroneuro.2008.12.006
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0924977X08003131
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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