Comparison of four commercial screening assays for the diagnosis of human T-cell Lymphotropic virus types 1 and 2

Autores
Berini, Carolina Andrea; Susana Pascuccio, M.; Bautista, Christian T.; Gendler, Silvina A.; Eirin, Maria Emilia; Rodriguez, Claudia; Pando, María de los Ángeles; Biglione, Mirna Marcela
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Serological assays for human T-cell lymphotropic virus types 1 and 2 (HTLV-1/2) are widely used in routine screening of blood donors. The aim of this study was to compare the performance of four commercial screening assays for HTLV-1/2 infection frequently used in South America. A total of 142 HTLV-1 and HTLV-2 seropositive and 336 seronegative samples were analyzed by using four commercial tests (BioKit, Vironostika, Murex and Fujirebio). These tests are commonly used for HTLV-1/2 detection in blood banks in Argentina. A nested-PCR was used as the reference standard. The most sensitive tests for HTLV-1/2 were Fujirebio and Biokit (98.6%) followed by Murex (97.2%) and Vironostika (96.5%). The most specific test was Murex (99.7%), followed by Biokit (97.0%), Fujirebio (95.8%), and Vironostika (92.9%). The kappa index of agreement was higher for Murex (κ = 0.97), followed by BioKit (κ = 0.94), Fujirebio (κ = 0.92), and Vironostika (κ = 0.86). The highest index of agreement was shown by Murex test while Vironostika had the lowest performance. Of the four tests evaluated, only the Vironostika assay is approved by the Food and Drug Administration. These results should be considered for choosing the most accurate serological screening assays in order to obtain an optimal efficiency of the current algorithm for HTLV-1/2 diagnosis.
Fil: Berini, Carolina Andrea. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia para el Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Susana Pascuccio, M.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; Argentina
Fil: Bautista, Christian T.. Asociacion Benefica Prisma Lima; Perú
Fil: Gendler, Silvina A.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; Argentina
Fil: Eirin, Maria Emilia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia para el Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Rodriguez, Claudia. Hospital de Alta Complejidad “Presidente Juan D. Perón”; Argentina
Fil: Pando, María de los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia para el Sida; Argentina
Fil: Biglione, Mirna Marcela. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia para el Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
ASSAYS
COMPARISON
HTLV-1/2
SENSITIVITY
SPECIFICITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/112833
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/112833
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparison of four commercial screening assays for the diagnosis of human T-cell Lymphotropic virus types 1 and 2Berini, Carolina AndreaSusana Pascuccio, M.Bautista, Christian T.Gendler, Silvina A.Eirin, Maria EmiliaRodriguez, ClaudiaPando, María de los ÁngelesBiglione, Mirna MarcelaASSAYSCOMPARISONHTLV-1/2SENSITIVITYSPECIFICITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Serological assays for human T-cell lymphotropic virus types 1 and 2 (HTLV-1/2) are widely used in routine screening of blood donors. The aim of this study was to compare the performance of four commercial screening assays for HTLV-1/2 infection frequently used in South America. A total of 142 HTLV-1 and HTLV-2 seropositive and 336 seronegative samples were analyzed by using four commercial tests (BioKit, Vironostika, Murex and Fujirebio). These tests are commonly used for HTLV-1/2 detection in blood banks in Argentina. A nested-PCR was used as the reference standard. The most sensitive tests for HTLV-1/2 were Fujirebio and Biokit (98.6%) followed by Murex (97.2%) and Vironostika (96.5%). The most specific test was Murex (99.7%), followed by Biokit (97.0%), Fujirebio (95.8%), and Vironostika (92.9%). The kappa index of agreement was higher for Murex (κ = 0.97), followed by BioKit (κ = 0.94), Fujirebio (κ = 0.92), and Vironostika (κ = 0.86). The highest index of agreement was shown by Murex test while Vironostika had the lowest performance. Of the four tests evaluated, only the Vironostika assay is approved by the Food and Drug Administration. These results should be considered for choosing the most accurate serological screening assays in order to obtain an optimal efficiency of the current algorithm for HTLV-1/2 diagnosis.Fil: Berini, Carolina Andrea. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia para el Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Susana Pascuccio, M.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; ArgentinaFil: Bautista, Christian T.. Asociacion Benefica Prisma Lima; PerúFil: Gendler, Silvina A.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; ArgentinaFil: Eirin, Maria Emilia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia para el Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Rodriguez, Claudia. Hospital de Alta Complejidad “Presidente Juan D. Perón”; ArgentinaFil: Pando, María de los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia para el Sida; ArgentinaFil: Biglione, Mirna Marcela. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia para el Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaElsevier Science2008-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/112833Berini, Carolina Andrea; Susana Pascuccio, M.; Bautista, Christian T.; Gendler, Silvina A.; Eirin, Maria Emilia; et al.; Comparison of four commercial screening assays for the diagnosis of human T-cell Lymphotropic virus types 1 and 2; Elsevier Science; Journal of Virological Methods; 147; 2; 2-2008; 322-3270166-0934CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2007.09.012info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0166093407003734info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:42Zoai:ri.conicet.gov.ar:11336/112833instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:42.717CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparison of four commercial screening assays for the diagnosis of human T-cell Lymphotropic virus types 1 and 2
title Comparison of four commercial screening assays for the diagnosis of human T-cell Lymphotropic virus types 1 and 2
spellingShingle Comparison of four commercial screening assays for the diagnosis of human T-cell Lymphotropic virus types 1 and 2
Berini, Carolina Andrea
ASSAYS
COMPARISON
HTLV-1/2
SENSITIVITY
SPECIFICITY
title_short Comparison of four commercial screening assays for the diagnosis of human T-cell Lymphotropic virus types 1 and 2
title_full Comparison of four commercial screening assays for the diagnosis of human T-cell Lymphotropic virus types 1 and 2
title_fullStr Comparison of four commercial screening assays for the diagnosis of human T-cell Lymphotropic virus types 1 and 2
title_full_unstemmed Comparison of four commercial screening assays for the diagnosis of human T-cell Lymphotropic virus types 1 and 2
title_sort Comparison of four commercial screening assays for the diagnosis of human T-cell Lymphotropic virus types 1 and 2
dc.creator.none.fl_str_mv Berini, Carolina Andrea
Susana Pascuccio, M.
Bautista, Christian T.
Gendler, Silvina A.
Eirin, Maria Emilia
Rodriguez, Claudia
Pando, María de los Ángeles
Biglione, Mirna Marcela
author Berini, Carolina Andrea
author_facet Berini, Carolina Andrea
Susana Pascuccio, M.
Bautista, Christian T.
Gendler, Silvina A.
Eirin, Maria Emilia
Rodriguez, Claudia
Pando, María de los Ángeles
Biglione, Mirna Marcela
author_role author
author2 Susana Pascuccio, M.
Bautista, Christian T.
Gendler, Silvina A.
Eirin, Maria Emilia
Rodriguez, Claudia
Pando, María de los Ángeles
Biglione, Mirna Marcela
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ASSAYS
COMPARISON
HTLV-1/2
SENSITIVITY
SPECIFICITY
topic ASSAYS
COMPARISON
HTLV-1/2
SENSITIVITY
SPECIFICITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Serological assays for human T-cell lymphotropic virus types 1 and 2 (HTLV-1/2) are widely used in routine screening of blood donors. The aim of this study was to compare the performance of four commercial screening assays for HTLV-1/2 infection frequently used in South America. A total of 142 HTLV-1 and HTLV-2 seropositive and 336 seronegative samples were analyzed by using four commercial tests (BioKit, Vironostika, Murex and Fujirebio). These tests are commonly used for HTLV-1/2 detection in blood banks in Argentina. A nested-PCR was used as the reference standard. The most sensitive tests for HTLV-1/2 were Fujirebio and Biokit (98.6%) followed by Murex (97.2%) and Vironostika (96.5%). The most specific test was Murex (99.7%), followed by Biokit (97.0%), Fujirebio (95.8%), and Vironostika (92.9%). The kappa index of agreement was higher for Murex (κ = 0.97), followed by BioKit (κ = 0.94), Fujirebio (κ = 0.92), and Vironostika (κ = 0.86). The highest index of agreement was shown by Murex test while Vironostika had the lowest performance. Of the four tests evaluated, only the Vironostika assay is approved by the Food and Drug Administration. These results should be considered for choosing the most accurate serological screening assays in order to obtain an optimal efficiency of the current algorithm for HTLV-1/2 diagnosis.
Fil: Berini, Carolina Andrea. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia para el Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Susana Pascuccio, M.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; Argentina
Fil: Bautista, Christian T.. Asociacion Benefica Prisma Lima; Perú
Fil: Gendler, Silvina A.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; Argentina
Fil: Eirin, Maria Emilia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia para el Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Rodriguez, Claudia. Hospital de Alta Complejidad “Presidente Juan D. Perón”; Argentina
Fil: Pando, María de los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia para el Sida; Argentina
Fil: Biglione, Mirna Marcela. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Centro Nacional de Referencia para el Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description Serological assays for human T-cell lymphotropic virus types 1 and 2 (HTLV-1/2) are widely used in routine screening of blood donors. The aim of this study was to compare the performance of four commercial screening assays for HTLV-1/2 infection frequently used in South America. A total of 142 HTLV-1 and HTLV-2 seropositive and 336 seronegative samples were analyzed by using four commercial tests (BioKit, Vironostika, Murex and Fujirebio). These tests are commonly used for HTLV-1/2 detection in blood banks in Argentina. A nested-PCR was used as the reference standard. The most sensitive tests for HTLV-1/2 were Fujirebio and Biokit (98.6%) followed by Murex (97.2%) and Vironostika (96.5%). The most specific test was Murex (99.7%), followed by Biokit (97.0%), Fujirebio (95.8%), and Vironostika (92.9%). The kappa index of agreement was higher for Murex (κ = 0.97), followed by BioKit (κ = 0.94), Fujirebio (κ = 0.92), and Vironostika (κ = 0.86). The highest index of agreement was shown by Murex test while Vironostika had the lowest performance. Of the four tests evaluated, only the Vironostika assay is approved by the Food and Drug Administration. These results should be considered for choosing the most accurate serological screening assays in order to obtain an optimal efficiency of the current algorithm for HTLV-1/2 diagnosis.
publishDate 2008
dc.date.none.fl_str_mv 2008-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/112833
Berini, Carolina Andrea; Susana Pascuccio, M.; Bautista, Christian T.; Gendler, Silvina A.; Eirin, Maria Emilia; et al.; Comparison of four commercial screening assays for the diagnosis of human T-cell Lymphotropic virus types 1 and 2; Elsevier Science; Journal of Virological Methods; 147; 2; 2-2008; 322-327
0166-0934
CONICET Digital
CONICET
url http://hdl.handle.net/11336/112833
identifier_str_mv Berini, Carolina Andrea; Susana Pascuccio, M.; Bautista, Christian T.; Gendler, Silvina A.; Eirin, Maria Emilia; et al.; Comparison of four commercial screening assays for the diagnosis of human T-cell Lymphotropic virus types 1 and 2; Elsevier Science; Journal of Virological Methods; 147; 2; 2-2008; 322-327
0166-0934
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2007.09.012
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0166093407003734
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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