Apolipoprotein A-I Treatment on Thp1 Macrophages Result in the Accumulation of Sequestosome 1 (p62) via Nrf2 Dependent

Autores
Díaz Ludovico, Ivo; Trejo, Sebastian Alejandro; Becerra, Romina Valeria; Garda, Horacio Alberto; Gonzalez, Marina Cecilia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The human apolipoprotein A-I (apoA-I) is the major protein component of high-density lipoproteins (HDL). It?s known that the interaction of apoA-I with macrophages trigger several signal transduction pathways. We realized a total proteomics profile between apoA-I and control treatment on THP-1 macrophages in order to find differential protein levels.To address if some unknown pathway could be activated by apoA-I we have analyzed a total proteomics profiling by comparative LFQ proteomics of apoA-I treated macrophages in comparison with untreated control.Total protein extracts were analyzed in a quadrupole/orbitrap. Subsequently, spectra were identified by two different software. Differential proteins levels were confirmed by Western-blot.Between 10 differentially-expressed proteins (at least in 2 folds over expressed on apoA-I-treated cells; p≤0.05) we found sequestrosome-1 (p62), a multifunctional protein that could be regulated by the Nrf2-KEAP1 pathway. When the Nrf2 pathway was inhibited by all-trans retinoic acid (ATRA), p62 levels decreases 40 to 50% in co treated cells (ATRA + ApoA-I) compared with only apoA-I-treated cells.We conclude that p62 could accumulates in THP-1-macrophages since apoA-I treatment in a Nrf2 pathway dependent manner.
Fil: Díaz Ludovico, Ivo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Trejo, Sebastian Alejandro. YPF - Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Becerra, Romina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Garda, Horacio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Gonzalez, Marina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Vascular Discovery: From Genes to Medicine Scientific Sessions 2020
Estados Unidos
American Heart Association
Materia
apolipoprotein A-I
high-density lipoproteins
sequestrosome-1
THP-1-macrophages
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/278231
Acceder
id CONICETDig_5407a23db404c7c392d88b05a5a6b761
oai_identifier_str oai:ri.conicet.gov.ar:11336/278231
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Apolipoprotein A-I Treatment on Thp1 Macrophages Result in the Accumulation of Sequestosome 1 (p62) via Nrf2 DependentDíaz Ludovico, IvoTrejo, Sebastian AlejandroBecerra, Romina ValeriaGarda, Horacio AlbertoGonzalez, Marina Ceciliaapolipoprotein A-Ihigh-density lipoproteinssequestrosome-1THP-1-macrophageshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The human apolipoprotein A-I (apoA-I) is the major protein component of high-density lipoproteins (HDL). It?s known that the interaction of apoA-I with macrophages trigger several signal transduction pathways. We realized a total proteomics profile between apoA-I and control treatment on THP-1 macrophages in order to find differential protein levels.To address if some unknown pathway could be activated by apoA-I we have analyzed a total proteomics profiling by comparative LFQ proteomics of apoA-I treated macrophages in comparison with untreated control.Total protein extracts were analyzed in a quadrupole/orbitrap. Subsequently, spectra were identified by two different software. Differential proteins levels were confirmed by Western-blot.Between 10 differentially-expressed proteins (at least in 2 folds over expressed on apoA-I-treated cells; p≤0.05) we found sequestrosome-1 (p62), a multifunctional protein that could be regulated by the Nrf2-KEAP1 pathway. When the Nrf2 pathway was inhibited by all-trans retinoic acid (ATRA), p62 levels decreases 40 to 50% in co treated cells (ATRA + ApoA-I) compared with only apoA-I-treated cells.We conclude that p62 could accumulates in THP-1-macrophages since apoA-I treatment in a Nrf2 pathway dependent manner.Fil: Díaz Ludovico, Ivo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Trejo, Sebastian Alejandro. YPF - Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Becerra, Romina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Garda, Horacio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Gonzalez, Marina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaVascular Discovery: From Genes to Medicine Scientific Sessions 2020Estados UnidosAmerican Heart AssociationAmerican Heart Association2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/278231Apolipoprotein A-I Treatment on Thp1 Macrophages Result in the Accumulation of Sequestosome 1 (p62) via Nrf2 Dependent; Vascular Discovery: From Genes to Medicine Scientific Sessions 2020; Estados Unidos; 2020; 1-11079-5642CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1161/atvb.40.suppl_1.307info:eu-repo/semantics/altIdentifier/url/https://www.ahajournals.org/doi/10.1161/atvb.40.suppl_1.307Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T13:20:51Zoai:ri.conicet.gov.ar:11336/278231instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 13:20:51.314CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Apolipoprotein A-I Treatment on Thp1 Macrophages Result in the Accumulation of Sequestosome 1 (p62) via Nrf2 Dependent
title Apolipoprotein A-I Treatment on Thp1 Macrophages Result in the Accumulation of Sequestosome 1 (p62) via Nrf2 Dependent
spellingShingle Apolipoprotein A-I Treatment on Thp1 Macrophages Result in the Accumulation of Sequestosome 1 (p62) via Nrf2 Dependent
Díaz Ludovico, Ivo
apolipoprotein A-I
high-density lipoproteins
sequestrosome-1
THP-1-macrophages
title_short Apolipoprotein A-I Treatment on Thp1 Macrophages Result in the Accumulation of Sequestosome 1 (p62) via Nrf2 Dependent
title_full Apolipoprotein A-I Treatment on Thp1 Macrophages Result in the Accumulation of Sequestosome 1 (p62) via Nrf2 Dependent
title_fullStr Apolipoprotein A-I Treatment on Thp1 Macrophages Result in the Accumulation of Sequestosome 1 (p62) via Nrf2 Dependent
title_full_unstemmed Apolipoprotein A-I Treatment on Thp1 Macrophages Result in the Accumulation of Sequestosome 1 (p62) via Nrf2 Dependent
title_sort Apolipoprotein A-I Treatment on Thp1 Macrophages Result in the Accumulation of Sequestosome 1 (p62) via Nrf2 Dependent
dc.creator.none.fl_str_mv Díaz Ludovico, Ivo
Trejo, Sebastian Alejandro
Becerra, Romina Valeria
Garda, Horacio Alberto
Gonzalez, Marina Cecilia
author Díaz Ludovico, Ivo
author_facet Díaz Ludovico, Ivo
Trejo, Sebastian Alejandro
Becerra, Romina Valeria
Garda, Horacio Alberto
Gonzalez, Marina Cecilia
author_role author
author2 Trejo, Sebastian Alejandro
Becerra, Romina Valeria
Garda, Horacio Alberto
Gonzalez, Marina Cecilia
author2_role author
author
author
author
dc.subject.none.fl_str_mv apolipoprotein A-I
high-density lipoproteins
sequestrosome-1
THP-1-macrophages
topic apolipoprotein A-I
high-density lipoproteins
sequestrosome-1
THP-1-macrophages
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The human apolipoprotein A-I (apoA-I) is the major protein component of high-density lipoproteins (HDL). It?s known that the interaction of apoA-I with macrophages trigger several signal transduction pathways. We realized a total proteomics profile between apoA-I and control treatment on THP-1 macrophages in order to find differential protein levels.To address if some unknown pathway could be activated by apoA-I we have analyzed a total proteomics profiling by comparative LFQ proteomics of apoA-I treated macrophages in comparison with untreated control.Total protein extracts were analyzed in a quadrupole/orbitrap. Subsequently, spectra were identified by two different software. Differential proteins levels were confirmed by Western-blot.Between 10 differentially-expressed proteins (at least in 2 folds over expressed on apoA-I-treated cells; p≤0.05) we found sequestrosome-1 (p62), a multifunctional protein that could be regulated by the Nrf2-KEAP1 pathway. When the Nrf2 pathway was inhibited by all-trans retinoic acid (ATRA), p62 levels decreases 40 to 50% in co treated cells (ATRA + ApoA-I) compared with only apoA-I-treated cells.We conclude that p62 could accumulates in THP-1-macrophages since apoA-I treatment in a Nrf2 pathway dependent manner.
Fil: Díaz Ludovico, Ivo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Trejo, Sebastian Alejandro. YPF - Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Becerra, Romina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Garda, Horacio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Gonzalez, Marina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Vascular Discovery: From Genes to Medicine Scientific Sessions 2020
Estados Unidos
American Heart Association
description The human apolipoprotein A-I (apoA-I) is the major protein component of high-density lipoproteins (HDL). It?s known that the interaction of apoA-I with macrophages trigger several signal transduction pathways. We realized a total proteomics profile between apoA-I and control treatment on THP-1 macrophages in order to find differential protein levels.To address if some unknown pathway could be activated by apoA-I we have analyzed a total proteomics profiling by comparative LFQ proteomics of apoA-I treated macrophages in comparison with untreated control.Total protein extracts were analyzed in a quadrupole/orbitrap. Subsequently, spectra were identified by two different software. Differential proteins levels were confirmed by Western-blot.Between 10 differentially-expressed proteins (at least in 2 folds over expressed on apoA-I-treated cells; p≤0.05) we found sequestrosome-1 (p62), a multifunctional protein that could be regulated by the Nrf2-KEAP1 pathway. When the Nrf2 pathway was inhibited by all-trans retinoic acid (ATRA), p62 levels decreases 40 to 50% in co treated cells (ATRA + ApoA-I) compared with only apoA-I-treated cells.We conclude that p62 could accumulates in THP-1-macrophages since apoA-I treatment in a Nrf2 pathway dependent manner.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/278231
Apolipoprotein A-I Treatment on Thp1 Macrophages Result in the Accumulation of Sequestosome 1 (p62) via Nrf2 Dependent; Vascular Discovery: From Genes to Medicine Scientific Sessions 2020; Estados Unidos; 2020; 1-1
1079-5642
CONICET Digital
CONICET
url http://hdl.handle.net/11336/278231
identifier_str_mv Apolipoprotein A-I Treatment on Thp1 Macrophages Result in the Accumulation of Sequestosome 1 (p62) via Nrf2 Dependent; Vascular Discovery: From Genes to Medicine Scientific Sessions 2020; Estados Unidos; 2020; 1-1
1079-5642
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1161/atvb.40.suppl_1.307
info:eu-repo/semantics/altIdentifier/url/https://www.ahajournals.org/doi/10.1161/atvb.40.suppl_1.307
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv American Heart Association
publisher.none.fl_str_mv American Heart Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.952241

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