Assessment of alternative sources of seaweed polysaccharides in Argentina: potentials of the agarophyte Gelidium crinale (Hare ex Turner) Gaillon (Rhodophyta, Gelidiales)

Autores
Croce, Maria Emilia; Villar, Marcelo Armando; Parodi, Elisa Rosalia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The phycocolloids industry in Argentina has been based on the importation of non-native species and the exploitation of natural populations of Gracilaria, which are currently declining. This study was carried out to evaluate the potential of Gelidium crinale as an unexploited source of agar in this country. The yield and composition of polysaccharides extracted from G. crinale, as well as the growth potential of this species were studied. In vitro cultures of apical sections were carried out; relative growth rates (RGR) and data were analyzed through repeated measurements. Explants rapidly duplicated their initial size, producing numerous proliferations and achieving structural complexity. RGR of explants, primary proliferations, and secondary proliferations were high and similar. Polysaccharide extraction was done under three conditions: acid pre-extraction treatment + pressure boiled (AP), water pre-extraction treatment + pressure boiled (WP), and water pre-extraction treatment + boiled (WB). Polysaccharides were characterized through infrared spectroscopy, X-ray fluorescence, thermogravimetric, and elemental analysis. Polysaccharides obtained corresponded to the agar-type, and showed high similarity to commercial agar samples, with low sulfate and methyl contents. Higher yields were obtained with pressure extractions; however, acid treatment did not increase the amount of extracted biopolymers. Their degradation occurred in the range 250–400 °C. Polysaccharides obtained under AP conditions had a degradation curve similar to food-grade agar-agar, whereas degradation curves of polysaccharides obtained without acid treatment were similar to biotechnology-grade agar-agar. According to the results, G. crinale from Anegada Bay produces a high-quality agar to be considered for the Argentinean agar industry; however, micropropagation techniques would be indispensable to increase its bioavailability.
Fil: Croce, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); Argentina
Fil: Villar, Marcelo Armando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina
Fil: Parodi, Elisa Rosalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ecología Acuática; Argentina
Materia
Agar
Apices
Ft-Ir
In Vitro Culture
Relative Growth Rate
Thermogravimetry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12522
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/12522
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Assessment of alternative sources of seaweed polysaccharides in Argentina: potentials of the agarophyte Gelidium crinale (Hare ex Turner) Gaillon (Rhodophyta, Gelidiales)Croce, Maria EmiliaVillar, Marcelo ArmandoParodi, Elisa RosaliaAgarApicesFt-IrIn Vitro CultureRelative Growth RateThermogravimetryhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The phycocolloids industry in Argentina has been based on the importation of non-native species and the exploitation of natural populations of Gracilaria, which are currently declining. This study was carried out to evaluate the potential of Gelidium crinale as an unexploited source of agar in this country. The yield and composition of polysaccharides extracted from G. crinale, as well as the growth potential of this species were studied. In vitro cultures of apical sections were carried out; relative growth rates (RGR) and data were analyzed through repeated measurements. Explants rapidly duplicated their initial size, producing numerous proliferations and achieving structural complexity. RGR of explants, primary proliferations, and secondary proliferations were high and similar. Polysaccharide extraction was done under three conditions: acid pre-extraction treatment + pressure boiled (AP), water pre-extraction treatment + pressure boiled (WP), and water pre-extraction treatment + boiled (WB). Polysaccharides were characterized through infrared spectroscopy, X-ray fluorescence, thermogravimetric, and elemental analysis. Polysaccharides obtained corresponded to the agar-type, and showed high similarity to commercial agar samples, with low sulfate and methyl contents. Higher yields were obtained with pressure extractions; however, acid treatment did not increase the amount of extracted biopolymers. Their degradation occurred in the range 250–400 °C. Polysaccharides obtained under AP conditions had a degradation curve similar to food-grade agar-agar, whereas degradation curves of polysaccharides obtained without acid treatment were similar to biotechnology-grade agar-agar. According to the results, G. crinale from Anegada Bay produces a high-quality agar to be considered for the Argentinean agar industry; however, micropropagation techniques would be indispensable to increase its bioavailability.Fil: Croce, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); ArgentinaFil: Villar, Marcelo Armando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); ArgentinaFil: Parodi, Elisa Rosalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ecología Acuática; ArgentinaSpringer2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12522Croce, Maria Emilia; Villar, Marcelo Armando; Parodi, Elisa Rosalia; Assessment of alternative sources of seaweed polysaccharides in Argentina: potentials of the agarophyte Gelidium crinale (Hare ex Turner) Gaillon (Rhodophyta, Gelidiales); Springer; Journal Of Applied Phycology; 27; 5; 10-2015; 2099-21100921-89711573-5176enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs10811-015-0537-1info:eu-repo/semantics/altIdentifier/doi/10.1007/s10811-015-0537-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:35:39Zoai:ri.conicet.gov.ar:11336/12522instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:35:40.174CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Assessment of alternative sources of seaweed polysaccharides in Argentina: potentials of the agarophyte Gelidium crinale (Hare ex Turner) Gaillon (Rhodophyta, Gelidiales)
title Assessment of alternative sources of seaweed polysaccharides in Argentina: potentials of the agarophyte Gelidium crinale (Hare ex Turner) Gaillon (Rhodophyta, Gelidiales)
spellingShingle Assessment of alternative sources of seaweed polysaccharides in Argentina: potentials of the agarophyte Gelidium crinale (Hare ex Turner) Gaillon (Rhodophyta, Gelidiales)
Croce, Maria Emilia
Agar
Apices
Ft-Ir
In Vitro Culture
Relative Growth Rate
Thermogravimetry
title_short Assessment of alternative sources of seaweed polysaccharides in Argentina: potentials of the agarophyte Gelidium crinale (Hare ex Turner) Gaillon (Rhodophyta, Gelidiales)
title_full Assessment of alternative sources of seaweed polysaccharides in Argentina: potentials of the agarophyte Gelidium crinale (Hare ex Turner) Gaillon (Rhodophyta, Gelidiales)
title_fullStr Assessment of alternative sources of seaweed polysaccharides in Argentina: potentials of the agarophyte Gelidium crinale (Hare ex Turner) Gaillon (Rhodophyta, Gelidiales)
title_full_unstemmed Assessment of alternative sources of seaweed polysaccharides in Argentina: potentials of the agarophyte Gelidium crinale (Hare ex Turner) Gaillon (Rhodophyta, Gelidiales)
title_sort Assessment of alternative sources of seaweed polysaccharides in Argentina: potentials of the agarophyte Gelidium crinale (Hare ex Turner) Gaillon (Rhodophyta, Gelidiales)
dc.creator.none.fl_str_mv Croce, Maria Emilia
Villar, Marcelo Armando
Parodi, Elisa Rosalia
author Croce, Maria Emilia
author_facet Croce, Maria Emilia
Villar, Marcelo Armando
Parodi, Elisa Rosalia
author_role author
author2 Villar, Marcelo Armando
Parodi, Elisa Rosalia
author2_role author
author
dc.subject.none.fl_str_mv Agar
Apices
Ft-Ir
In Vitro Culture
Relative Growth Rate
Thermogravimetry
topic Agar
Apices
Ft-Ir
In Vitro Culture
Relative Growth Rate
Thermogravimetry
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The phycocolloids industry in Argentina has been based on the importation of non-native species and the exploitation of natural populations of Gracilaria, which are currently declining. This study was carried out to evaluate the potential of Gelidium crinale as an unexploited source of agar in this country. The yield and composition of polysaccharides extracted from G. crinale, as well as the growth potential of this species were studied. In vitro cultures of apical sections were carried out; relative growth rates (RGR) and data were analyzed through repeated measurements. Explants rapidly duplicated their initial size, producing numerous proliferations and achieving structural complexity. RGR of explants, primary proliferations, and secondary proliferations were high and similar. Polysaccharide extraction was done under three conditions: acid pre-extraction treatment + pressure boiled (AP), water pre-extraction treatment + pressure boiled (WP), and water pre-extraction treatment + boiled (WB). Polysaccharides were characterized through infrared spectroscopy, X-ray fluorescence, thermogravimetric, and elemental analysis. Polysaccharides obtained corresponded to the agar-type, and showed high similarity to commercial agar samples, with low sulfate and methyl contents. Higher yields were obtained with pressure extractions; however, acid treatment did not increase the amount of extracted biopolymers. Their degradation occurred in the range 250–400 °C. Polysaccharides obtained under AP conditions had a degradation curve similar to food-grade agar-agar, whereas degradation curves of polysaccharides obtained without acid treatment were similar to biotechnology-grade agar-agar. According to the results, G. crinale from Anegada Bay produces a high-quality agar to be considered for the Argentinean agar industry; however, micropropagation techniques would be indispensable to increase its bioavailability.
Fil: Croce, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); Argentina
Fil: Villar, Marcelo Armando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina
Fil: Parodi, Elisa Rosalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ecología Acuática; Argentina
description The phycocolloids industry in Argentina has been based on the importation of non-native species and the exploitation of natural populations of Gracilaria, which are currently declining. This study was carried out to evaluate the potential of Gelidium crinale as an unexploited source of agar in this country. The yield and composition of polysaccharides extracted from G. crinale, as well as the growth potential of this species were studied. In vitro cultures of apical sections were carried out; relative growth rates (RGR) and data were analyzed through repeated measurements. Explants rapidly duplicated their initial size, producing numerous proliferations and achieving structural complexity. RGR of explants, primary proliferations, and secondary proliferations were high and similar. Polysaccharide extraction was done under three conditions: acid pre-extraction treatment + pressure boiled (AP), water pre-extraction treatment + pressure boiled (WP), and water pre-extraction treatment + boiled (WB). Polysaccharides were characterized through infrared spectroscopy, X-ray fluorescence, thermogravimetric, and elemental analysis. Polysaccharides obtained corresponded to the agar-type, and showed high similarity to commercial agar samples, with low sulfate and methyl contents. Higher yields were obtained with pressure extractions; however, acid treatment did not increase the amount of extracted biopolymers. Their degradation occurred in the range 250–400 °C. Polysaccharides obtained under AP conditions had a degradation curve similar to food-grade agar-agar, whereas degradation curves of polysaccharides obtained without acid treatment were similar to biotechnology-grade agar-agar. According to the results, G. crinale from Anegada Bay produces a high-quality agar to be considered for the Argentinean agar industry; however, micropropagation techniques would be indispensable to increase its bioavailability.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12522
Croce, Maria Emilia; Villar, Marcelo Armando; Parodi, Elisa Rosalia; Assessment of alternative sources of seaweed polysaccharides in Argentina: potentials of the agarophyte Gelidium crinale (Hare ex Turner) Gaillon (Rhodophyta, Gelidiales); Springer; Journal Of Applied Phycology; 27; 5; 10-2015; 2099-2110
0921-8971
1573-5176
url http://hdl.handle.net/11336/12522
identifier_str_mv Croce, Maria Emilia; Villar, Marcelo Armando; Parodi, Elisa Rosalia; Assessment of alternative sources of seaweed polysaccharides in Argentina: potentials of the agarophyte Gelidium crinale (Hare ex Turner) Gaillon (Rhodophyta, Gelidiales); Springer; Journal Of Applied Phycology; 27; 5; 10-2015; 2099-2110
0921-8971
1573-5176
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs10811-015-0537-1
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10811-015-0537-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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