Reply to Kao and Liaw

Autores
Ochoa, Valeria Pilar; Erra Diaz, Fernando Alberto; Ramirez, Joaquin Ezequiel; Fentini, María Clara; Carobene, Mauricio; Geffner, Jorge Raúl; Arruvito, Maria Lourdes; Remes Lenicov, Federico
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The letter by Kao and Liaw raises some interesting observations. However, none ofthem question the results and conclusions of our study. As a first point, theyrecommend the use of fecal specimens to avoid, in their words, an uncertainty inthe accuracy of RT-PCR from respiratory samples. While it has been reported thatfecal samples might provide increased sensibility to detect SARS-CoV-2 at laterstages of infection, no evidence supports that data collected in this way were moreaccurate or relevant in terms of diagnosis or transmission.Secondly, Kao and Liaw raise the issue of the time elapsed between symptom onsetand RT-PCR test. This is a well-known confounder in viral load comparisons, andas such, it was clearly acknowledged in our study. We showed that the periodbetween symptom onset and diagnosis were not significantly different among thegroups studied. In fact, there were no cases with more than 7 days between theRT-PCR test and symptom onset in the population under 10 years old, and theproportion was negligible for the older population.Finally, we share the interest of Kao and Liaw about the possible association ofrespiratory viral loads with the expression of host genes related to mucosalimmunity. The search for these associations was not in the scope of our study, butwe agree they could be informative about the mechanisms that control respiratoryviral loads.All in all, by studying the SARS-CoV-2 viral loads of 45,318 respiratory samplesfrom COVID-19 patients measured by a validated RT-PCR method, our studyclearly demonstrates that infants younger than 6 months have higher viral loadsthan any other age group.
Fil: Ochoa, Valeria Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Erra Diaz, Fernando Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Ramirez, Joaquin Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Fentini, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Carobene, Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Geffner, Jorge Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Arruvito, Maria Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Remes Lenicov, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Materia
COVID-19
SARS-COV-2
RT-PCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215742
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/215742
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network_name_str CONICET Digital (CONICET)
spelling Reply to Kao and LiawOchoa, Valeria PilarErra Diaz, Fernando AlbertoRamirez, Joaquin EzequielFentini, María ClaraCarobene, MauricioGeffner, Jorge RaúlArruvito, Maria LourdesRemes Lenicov, FedericoCOVID-19SARS-COV-2RT-PCRhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The letter by Kao and Liaw raises some interesting observations. However, none ofthem question the results and conclusions of our study. As a first point, theyrecommend the use of fecal specimens to avoid, in their words, an uncertainty inthe accuracy of RT-PCR from respiratory samples. While it has been reported thatfecal samples might provide increased sensibility to detect SARS-CoV-2 at laterstages of infection, no evidence supports that data collected in this way were moreaccurate or relevant in terms of diagnosis or transmission.Secondly, Kao and Liaw raise the issue of the time elapsed between symptom onsetand RT-PCR test. This is a well-known confounder in viral load comparisons, andas such, it was clearly acknowledged in our study. We showed that the periodbetween symptom onset and diagnosis were not significantly different among thegroups studied. In fact, there were no cases with more than 7 days between theRT-PCR test and symptom onset in the population under 10 years old, and theproportion was negligible for the older population.Finally, we share the interest of Kao and Liaw about the possible association ofrespiratory viral loads with the expression of host genes related to mucosalimmunity. The search for these associations was not in the scope of our study, butwe agree they could be informative about the mechanisms that control respiratoryviral loads.All in all, by studying the SARS-CoV-2 viral loads of 45,318 respiratory samplesfrom COVID-19 patients measured by a validated RT-PCR method, our studyclearly demonstrates that infants younger than 6 months have higher viral loadsthan any other age group.Fil: Ochoa, Valeria Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Erra Diaz, Fernando Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Ramirez, Joaquin Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Fentini, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Carobene, Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Geffner, Jorge Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Arruvito, Maria Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Remes Lenicov, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaUniversity of Chicago Press2022-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215742Ochoa, Valeria Pilar; Erra Diaz, Fernando Alberto; Ramirez, Joaquin Ezequiel; Fentini, María Clara; Carobene, Mauricio; et al.; Reply to Kao and Liaw; University of Chicago Press; Journal Of Infectious Diseases; 226; 1; 7-2022; 1-30022-1899CONICET DigitalCONICETenghttps://ri.conicet.gov.ar/handle/11336/149768info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jid/advance-article/doi/10.1093/infdis/jiac081/6542718info:eu-repo/semantics/altIdentifier/doi/10.1093/infdis/jiac081info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:37:18Zoai:ri.conicet.gov.ar:11336/215742instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:37:18.432CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Reply to Kao and Liaw
title Reply to Kao and Liaw
spellingShingle Reply to Kao and Liaw
Ochoa, Valeria Pilar
COVID-19
SARS-COV-2
RT-PCR
title_short Reply to Kao and Liaw
title_full Reply to Kao and Liaw
title_fullStr Reply to Kao and Liaw
title_full_unstemmed Reply to Kao and Liaw
title_sort Reply to Kao and Liaw
dc.creator.none.fl_str_mv Ochoa, Valeria Pilar
Erra Diaz, Fernando Alberto
Ramirez, Joaquin Ezequiel
Fentini, María Clara
Carobene, Mauricio
Geffner, Jorge Raúl
Arruvito, Maria Lourdes
Remes Lenicov, Federico
author Ochoa, Valeria Pilar
author_facet Ochoa, Valeria Pilar
Erra Diaz, Fernando Alberto
Ramirez, Joaquin Ezequiel
Fentini, María Clara
Carobene, Mauricio
Geffner, Jorge Raúl
Arruvito, Maria Lourdes
Remes Lenicov, Federico
author_role author
author2 Erra Diaz, Fernando Alberto
Ramirez, Joaquin Ezequiel
Fentini, María Clara
Carobene, Mauricio
Geffner, Jorge Raúl
Arruvito, Maria Lourdes
Remes Lenicov, Federico
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COVID-19
SARS-COV-2
RT-PCR
topic COVID-19
SARS-COV-2
RT-PCR
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The letter by Kao and Liaw raises some interesting observations. However, none ofthem question the results and conclusions of our study. As a first point, theyrecommend the use of fecal specimens to avoid, in their words, an uncertainty inthe accuracy of RT-PCR from respiratory samples. While it has been reported thatfecal samples might provide increased sensibility to detect SARS-CoV-2 at laterstages of infection, no evidence supports that data collected in this way were moreaccurate or relevant in terms of diagnosis or transmission.Secondly, Kao and Liaw raise the issue of the time elapsed between symptom onsetand RT-PCR test. This is a well-known confounder in viral load comparisons, andas such, it was clearly acknowledged in our study. We showed that the periodbetween symptom onset and diagnosis were not significantly different among thegroups studied. In fact, there were no cases with more than 7 days between theRT-PCR test and symptom onset in the population under 10 years old, and theproportion was negligible for the older population.Finally, we share the interest of Kao and Liaw about the possible association ofrespiratory viral loads with the expression of host genes related to mucosalimmunity. The search for these associations was not in the scope of our study, butwe agree they could be informative about the mechanisms that control respiratoryviral loads.All in all, by studying the SARS-CoV-2 viral loads of 45,318 respiratory samplesfrom COVID-19 patients measured by a validated RT-PCR method, our studyclearly demonstrates that infants younger than 6 months have higher viral loadsthan any other age group.
Fil: Ochoa, Valeria Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Erra Diaz, Fernando Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Ramirez, Joaquin Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Fentini, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Carobene, Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Geffner, Jorge Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Arruvito, Maria Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Remes Lenicov, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
description The letter by Kao and Liaw raises some interesting observations. However, none ofthem question the results and conclusions of our study. As a first point, theyrecommend the use of fecal specimens to avoid, in their words, an uncertainty inthe accuracy of RT-PCR from respiratory samples. While it has been reported thatfecal samples might provide increased sensibility to detect SARS-CoV-2 at laterstages of infection, no evidence supports that data collected in this way were moreaccurate or relevant in terms of diagnosis or transmission.Secondly, Kao and Liaw raise the issue of the time elapsed between symptom onsetand RT-PCR test. This is a well-known confounder in viral load comparisons, andas such, it was clearly acknowledged in our study. We showed that the periodbetween symptom onset and diagnosis were not significantly different among thegroups studied. In fact, there were no cases with more than 7 days between theRT-PCR test and symptom onset in the population under 10 years old, and theproportion was negligible for the older population.Finally, we share the interest of Kao and Liaw about the possible association ofrespiratory viral loads with the expression of host genes related to mucosalimmunity. The search for these associations was not in the scope of our study, butwe agree they could be informative about the mechanisms that control respiratoryviral loads.All in all, by studying the SARS-CoV-2 viral loads of 45,318 respiratory samplesfrom COVID-19 patients measured by a validated RT-PCR method, our studyclearly demonstrates that infants younger than 6 months have higher viral loadsthan any other age group.
publishDate 2022
dc.date.none.fl_str_mv 2022-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215742
Ochoa, Valeria Pilar; Erra Diaz, Fernando Alberto; Ramirez, Joaquin Ezequiel; Fentini, María Clara; Carobene, Mauricio; et al.; Reply to Kao and Liaw; University of Chicago Press; Journal Of Infectious Diseases; 226; 1; 7-2022; 1-3
0022-1899
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215742
identifier_str_mv Ochoa, Valeria Pilar; Erra Diaz, Fernando Alberto; Ramirez, Joaquin Ezequiel; Fentini, María Clara; Carobene, Mauricio; et al.; Reply to Kao and Liaw; University of Chicago Press; Journal Of Infectious Diseases; 226; 1; 7-2022; 1-3
0022-1899
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ri.conicet.gov.ar/handle/11336/149768
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jid/advance-article/doi/10.1093/infdis/jiac081/6542718
info:eu-repo/semantics/altIdentifier/doi/10.1093/infdis/jiac081
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv University of Chicago Press
publisher.none.fl_str_mv University of Chicago Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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