Phylogenetic relationships of closely-related phlebotomine sand flies (diptera: psychodidae) of nyssomyia genus and Lutzomyia subgenus
- Autores
- Pech May, Angélica del Rosario; Moya, Sofía Lorián; Quintana, María Gabriela; Manteca Acosta, Mariana; Salomón, Oscar Daniel
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- BACKGROUND The Nyssomyia genus and Lutzomyia subgenus include medical important species that are Latin American leishmaniases vectors. Little is known about the phylogenetic relationships of closely-related species in each of these taxonomic groups that are morphologically indistinguishable or differentiated by very subtle details.OBJECTIVES We inferred the phylogenetic relationships of closely-related species within both the Nyssomyia genus and the Lutzomyia subgenus using a cytochrome c oxidase subunit I (COI) fragment.METHODS The sampling was carried out from 11 Argentinean localities. For genetic analyses, we used GenBank sequences in addition to our sequences from Argentina. Kimura 2-parameter (K2P) genetic distance and nucleotide divergence (Da) was calculated between closely-related species of Nyssomyia genus, Lutzomyia subgenus and between clades of Lutzomyia longipalpis complex.FINDINGS The K2P and Da values within species of Nyssomyia genus and Lutzomyia subgenus were lower than the divergence detected between clades of Lu. longipalpis complex. The haplotype network analyses within Lutzomyia subgenus showed shared haplotypes between species, contrary to Nyssomyia genus with none haplotype shared. Bayesian inference within Nyssomyia genus presented structuring by species.MAIN CONCLUSIONS This study evidences the phylogenetic proximity among closely-related species within Nyssomyia genus and Lutzomyia subgenus. The COI sequences of Nyssomyia neivai derived from the present study are the first available in GenBank.
Fil: Pech May, Angélica del Rosario. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moya, Sofía Lorián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; Argentina
Fil: Quintana, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; Argentina
Fil: Manteca Acosta, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; Argentina
Fil: Salomón, Oscar Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; Argentina - Materia
-
PHLEBOTOMINE SAND FLIES
VECTORS
COI - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/116076
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/116076 |
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3498 |
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CONICET Digital (CONICET) |
spelling |
Phylogenetic relationships of closely-related phlebotomine sand flies (diptera: psychodidae) of nyssomyia genus and Lutzomyia subgenusPech May, Angélica del RosarioMoya, Sofía LoriánQuintana, María GabrielaManteca Acosta, MarianaSalomón, Oscar DanielPHLEBOTOMINE SAND FLIESVECTORSCOIhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1BACKGROUND The Nyssomyia genus and Lutzomyia subgenus include medical important species that are Latin American leishmaniases vectors. Little is known about the phylogenetic relationships of closely-related species in each of these taxonomic groups that are morphologically indistinguishable or differentiated by very subtle details.OBJECTIVES We inferred the phylogenetic relationships of closely-related species within both the Nyssomyia genus and the Lutzomyia subgenus using a cytochrome c oxidase subunit I (COI) fragment.METHODS The sampling was carried out from 11 Argentinean localities. For genetic analyses, we used GenBank sequences in addition to our sequences from Argentina. Kimura 2-parameter (K2P) genetic distance and nucleotide divergence (Da) was calculated between closely-related species of Nyssomyia genus, Lutzomyia subgenus and between clades of Lutzomyia longipalpis complex.FINDINGS The K2P and Da values within species of Nyssomyia genus and Lutzomyia subgenus were lower than the divergence detected between clades of Lu. longipalpis complex. The haplotype network analyses within Lutzomyia subgenus showed shared haplotypes between species, contrary to Nyssomyia genus with none haplotype shared. Bayesian inference within Nyssomyia genus presented structuring by species.MAIN CONCLUSIONS This study evidences the phylogenetic proximity among closely-related species within Nyssomyia genus and Lutzomyia subgenus. The COI sequences of Nyssomyia neivai derived from the present study are the first available in GenBank.Fil: Pech May, Angélica del Rosario. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moya, Sofía Lorián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; ArgentinaFil: Quintana, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; ArgentinaFil: Manteca Acosta, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; ArgentinaFil: Salomón, Oscar Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; ArgentinaFundação Oswaldo Cruz2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/116076Pech May, Angélica del Rosario; Moya, Sofía Lorián; Quintana, María Gabriela; Manteca Acosta, Mariana; Salomón, Oscar Daniel; Phylogenetic relationships of closely-related phlebotomine sand flies (diptera: psychodidae) of nyssomyia genus and Lutzomyia subgenus; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 115; 0; 8-2020; 1-100074-02761678-8060CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/ 10.1590/0074-02760200220info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762020000100338&tlng=eninfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:26:15Zoai:ri.conicet.gov.ar:11336/116076instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:26:16.157CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Phylogenetic relationships of closely-related phlebotomine sand flies (diptera: psychodidae) of nyssomyia genus and Lutzomyia subgenus |
title |
Phylogenetic relationships of closely-related phlebotomine sand flies (diptera: psychodidae) of nyssomyia genus and Lutzomyia subgenus |
spellingShingle |
Phylogenetic relationships of closely-related phlebotomine sand flies (diptera: psychodidae) of nyssomyia genus and Lutzomyia subgenus Pech May, Angélica del Rosario PHLEBOTOMINE SAND FLIES VECTORS COI |
title_short |
Phylogenetic relationships of closely-related phlebotomine sand flies (diptera: psychodidae) of nyssomyia genus and Lutzomyia subgenus |
title_full |
Phylogenetic relationships of closely-related phlebotomine sand flies (diptera: psychodidae) of nyssomyia genus and Lutzomyia subgenus |
title_fullStr |
Phylogenetic relationships of closely-related phlebotomine sand flies (diptera: psychodidae) of nyssomyia genus and Lutzomyia subgenus |
title_full_unstemmed |
Phylogenetic relationships of closely-related phlebotomine sand flies (diptera: psychodidae) of nyssomyia genus and Lutzomyia subgenus |
title_sort |
Phylogenetic relationships of closely-related phlebotomine sand flies (diptera: psychodidae) of nyssomyia genus and Lutzomyia subgenus |
dc.creator.none.fl_str_mv |
Pech May, Angélica del Rosario Moya, Sofía Lorián Quintana, María Gabriela Manteca Acosta, Mariana Salomón, Oscar Daniel |
author |
Pech May, Angélica del Rosario |
author_facet |
Pech May, Angélica del Rosario Moya, Sofía Lorián Quintana, María Gabriela Manteca Acosta, Mariana Salomón, Oscar Daniel |
author_role |
author |
author2 |
Moya, Sofía Lorián Quintana, María Gabriela Manteca Acosta, Mariana Salomón, Oscar Daniel |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
PHLEBOTOMINE SAND FLIES VECTORS COI |
topic |
PHLEBOTOMINE SAND FLIES VECTORS COI |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
BACKGROUND The Nyssomyia genus and Lutzomyia subgenus include medical important species that are Latin American leishmaniases vectors. Little is known about the phylogenetic relationships of closely-related species in each of these taxonomic groups that are morphologically indistinguishable or differentiated by very subtle details.OBJECTIVES We inferred the phylogenetic relationships of closely-related species within both the Nyssomyia genus and the Lutzomyia subgenus using a cytochrome c oxidase subunit I (COI) fragment.METHODS The sampling was carried out from 11 Argentinean localities. For genetic analyses, we used GenBank sequences in addition to our sequences from Argentina. Kimura 2-parameter (K2P) genetic distance and nucleotide divergence (Da) was calculated between closely-related species of Nyssomyia genus, Lutzomyia subgenus and between clades of Lutzomyia longipalpis complex.FINDINGS The K2P and Da values within species of Nyssomyia genus and Lutzomyia subgenus were lower than the divergence detected between clades of Lu. longipalpis complex. The haplotype network analyses within Lutzomyia subgenus showed shared haplotypes between species, contrary to Nyssomyia genus with none haplotype shared. Bayesian inference within Nyssomyia genus presented structuring by species.MAIN CONCLUSIONS This study evidences the phylogenetic proximity among closely-related species within Nyssomyia genus and Lutzomyia subgenus. The COI sequences of Nyssomyia neivai derived from the present study are the first available in GenBank. Fil: Pech May, Angélica del Rosario. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Moya, Sofía Lorián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; Argentina Fil: Quintana, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; Argentina Fil: Manteca Acosta, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; Argentina Fil: Salomón, Oscar Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; Argentina |
description |
BACKGROUND The Nyssomyia genus and Lutzomyia subgenus include medical important species that are Latin American leishmaniases vectors. Little is known about the phylogenetic relationships of closely-related species in each of these taxonomic groups that are morphologically indistinguishable or differentiated by very subtle details.OBJECTIVES We inferred the phylogenetic relationships of closely-related species within both the Nyssomyia genus and the Lutzomyia subgenus using a cytochrome c oxidase subunit I (COI) fragment.METHODS The sampling was carried out from 11 Argentinean localities. For genetic analyses, we used GenBank sequences in addition to our sequences from Argentina. Kimura 2-parameter (K2P) genetic distance and nucleotide divergence (Da) was calculated between closely-related species of Nyssomyia genus, Lutzomyia subgenus and between clades of Lutzomyia longipalpis complex.FINDINGS The K2P and Da values within species of Nyssomyia genus and Lutzomyia subgenus were lower than the divergence detected between clades of Lu. longipalpis complex. The haplotype network analyses within Lutzomyia subgenus showed shared haplotypes between species, contrary to Nyssomyia genus with none haplotype shared. Bayesian inference within Nyssomyia genus presented structuring by species.MAIN CONCLUSIONS This study evidences the phylogenetic proximity among closely-related species within Nyssomyia genus and Lutzomyia subgenus. The COI sequences of Nyssomyia neivai derived from the present study are the first available in GenBank. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/116076 Pech May, Angélica del Rosario; Moya, Sofía Lorián; Quintana, María Gabriela; Manteca Acosta, Mariana; Salomón, Oscar Daniel; Phylogenetic relationships of closely-related phlebotomine sand flies (diptera: psychodidae) of nyssomyia genus and Lutzomyia subgenus; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 115; 0; 8-2020; 1-10 0074-0276 1678-8060 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/116076 |
identifier_str_mv |
Pech May, Angélica del Rosario; Moya, Sofía Lorián; Quintana, María Gabriela; Manteca Acosta, Mariana; Salomón, Oscar Daniel; Phylogenetic relationships of closely-related phlebotomine sand flies (diptera: psychodidae) of nyssomyia genus and Lutzomyia subgenus; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 115; 0; 8-2020; 1-10 0074-0276 1678-8060 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/ 10.1590/0074-02760200220 info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762020000100338&tlng=en |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Fundação Oswaldo Cruz |
publisher.none.fl_str_mv |
Fundação Oswaldo Cruz |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846082706345033728 |
score |
13.22299 |