A complex tissue‐specific interplay between the Arabidopsis transcription factors AtMYB68 , AtHB23 , and AtPHL1 modulates primary and lateral root development and adaptation to sal...

Autores
Spies, Fiorella Paola; Perotti, María Florencia; Cho, Yuhan; Jo, Chang Ig; Chang Ig,; Chan, Raquel Lía
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adaptation to soil is a well-regulated process vital for plant life. AtHB23 is a homeodomain-leucine zipper I transcription factor (TF), previously revealed as crucial for plant survival in front of salinity conditions. We wondered whether this TF has partners to achieve this essential function. A TF cDNA library screening, Y2H, BiFC, and CoIP assays were complemented with expression analyses and phenotypic characterizations of silenced, mutant, overexpressor, and crossed plants in normal and salinity conditions. We revealed that AtHB23, AtPHL1, and AtMYB68 interact with each other, modulating root development and salinity response. The encoding genes coexpress in specific root tissues and developmental stages. In normal conditions, amiR68 silenced plants have less initiated roots, the opposite phenotype to that showed by amiR23 ones. AtMYB68 and AtPHL1 play contrary roles in lateral root elongation. Under salinity, where AtHB23 plays a crucial positive function, AtMYB68 cooperates with it, whereas AtPHL1 obstructs its action impacting survival ability and supporting the complex interaction between AtHB23, AtPHL1, and AtMYB68 in the primary and lateral roots. The root adaptation capability was associated with the amyloplast state. We identified new molecular players that through a complex relationship determine Arabidopsis root architecture and survival ability in salinity conditions.
Fil: Spies, Fiorella Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Perotti, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Cho, Yuhan. No especifíca;
Fil: Jo, Chang Ig. No especifíca;
Fil: Chang Ig,. University of Missouri; Estados Unidos
Fil: Chan, Raquel Lía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Materia
AtHB23
AtPHL1
AtMYB68
ROOT DEVELOPMENT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/241586
Acceder
id CONICETDig_52c2c59b3290a561324db15080806da6
oai_identifier_str oai:ri.conicet.gov.ar:11336/241586
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A complex tissue‐specific interplay between the Arabidopsis transcription factors AtMYB68 , AtHB23 , and AtPHL1 modulates primary and lateral root development and adaptation to salinitySpies, Fiorella PaolaPerotti, María FlorenciaCho, YuhanJo, Chang IgChang Ig,Chan, Raquel LíaAtHB23AtPHL1AtMYB68ROOT DEVELOPMENThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Adaptation to soil is a well-regulated process vital for plant life. AtHB23 is a homeodomain-leucine zipper I transcription factor (TF), previously revealed as crucial for plant survival in front of salinity conditions. We wondered whether this TF has partners to achieve this essential function. A TF cDNA library screening, Y2H, BiFC, and CoIP assays were complemented with expression analyses and phenotypic characterizations of silenced, mutant, overexpressor, and crossed plants in normal and salinity conditions. We revealed that AtHB23, AtPHL1, and AtMYB68 interact with each other, modulating root development and salinity response. The encoding genes coexpress in specific root tissues and developmental stages. In normal conditions, amiR68 silenced plants have less initiated roots, the opposite phenotype to that showed by amiR23 ones. AtMYB68 and AtPHL1 play contrary roles in lateral root elongation. Under salinity, where AtHB23 plays a crucial positive function, AtMYB68 cooperates with it, whereas AtPHL1 obstructs its action impacting survival ability and supporting the complex interaction between AtHB23, AtPHL1, and AtMYB68 in the primary and lateral roots. The root adaptation capability was associated with the amyloplast state. We identified new molecular players that through a complex relationship determine Arabidopsis root architecture and survival ability in salinity conditions.Fil: Spies, Fiorella Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Perotti, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Cho, Yuhan. No especifíca;Fil: Jo, Chang Ig. No especifíca;Fil: Chang Ig,. University of Missouri; Estados UnidosFil: Chan, Raquel Lía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaWiley Blackwell Publishing, Inc2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/241586Spies, Fiorella Paola; Perotti, María Florencia; Cho, Yuhan; Jo, Chang Ig; Chang Ig,; et al.; A complex tissue‐specific interplay between the Arabidopsis transcription factors AtMYB68 , AtHB23 , and AtPHL1 modulates primary and lateral root development and adaptation to salinity; Wiley Blackwell Publishing, Inc; Plant Journal; 115; 4; 5-2023; 952-9660960-7412CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/tpj.16273info:eu-repo/semantics/altIdentifier/doi/10.1111/tpj.16273info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:32:10Zoai:ri.conicet.gov.ar:11336/241586instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:32:10.563CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A complex tissue‐specific interplay between the Arabidopsis transcription factors AtMYB68 , AtHB23 , and AtPHL1 modulates primary and lateral root development and adaptation to salinity
title A complex tissue‐specific interplay between the Arabidopsis transcription factors AtMYB68 , AtHB23 , and AtPHL1 modulates primary and lateral root development and adaptation to salinity
spellingShingle A complex tissue‐specific interplay between the Arabidopsis transcription factors AtMYB68 , AtHB23 , and AtPHL1 modulates primary and lateral root development and adaptation to salinity
Spies, Fiorella Paola
AtHB23
AtPHL1
AtMYB68
ROOT DEVELOPMENT
title_short A complex tissue‐specific interplay between the Arabidopsis transcription factors AtMYB68 , AtHB23 , and AtPHL1 modulates primary and lateral root development and adaptation to salinity
title_full A complex tissue‐specific interplay between the Arabidopsis transcription factors AtMYB68 , AtHB23 , and AtPHL1 modulates primary and lateral root development and adaptation to salinity
title_fullStr A complex tissue‐specific interplay between the Arabidopsis transcription factors AtMYB68 , AtHB23 , and AtPHL1 modulates primary and lateral root development and adaptation to salinity
title_full_unstemmed A complex tissue‐specific interplay between the Arabidopsis transcription factors AtMYB68 , AtHB23 , and AtPHL1 modulates primary and lateral root development and adaptation to salinity
title_sort A complex tissue‐specific interplay between the Arabidopsis transcription factors AtMYB68 , AtHB23 , and AtPHL1 modulates primary and lateral root development and adaptation to salinity
dc.creator.none.fl_str_mv Spies, Fiorella Paola
Perotti, María Florencia
Cho, Yuhan
Jo, Chang Ig
Chang Ig,
Chan, Raquel Lía
author Spies, Fiorella Paola
author_facet Spies, Fiorella Paola
Perotti, María Florencia
Cho, Yuhan
Jo, Chang Ig
Chang Ig,
Chan, Raquel Lía
author_role author
author2 Perotti, María Florencia
Cho, Yuhan
Jo, Chang Ig
Chang Ig,
Chan, Raquel Lía
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv AtHB23
AtPHL1
AtMYB68
ROOT DEVELOPMENT
topic AtHB23
AtPHL1
AtMYB68
ROOT DEVELOPMENT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Adaptation to soil is a well-regulated process vital for plant life. AtHB23 is a homeodomain-leucine zipper I transcription factor (TF), previously revealed as crucial for plant survival in front of salinity conditions. We wondered whether this TF has partners to achieve this essential function. A TF cDNA library screening, Y2H, BiFC, and CoIP assays were complemented with expression analyses and phenotypic characterizations of silenced, mutant, overexpressor, and crossed plants in normal and salinity conditions. We revealed that AtHB23, AtPHL1, and AtMYB68 interact with each other, modulating root development and salinity response. The encoding genes coexpress in specific root tissues and developmental stages. In normal conditions, amiR68 silenced plants have less initiated roots, the opposite phenotype to that showed by amiR23 ones. AtMYB68 and AtPHL1 play contrary roles in lateral root elongation. Under salinity, where AtHB23 plays a crucial positive function, AtMYB68 cooperates with it, whereas AtPHL1 obstructs its action impacting survival ability and supporting the complex interaction between AtHB23, AtPHL1, and AtMYB68 in the primary and lateral roots. The root adaptation capability was associated with the amyloplast state. We identified new molecular players that through a complex relationship determine Arabidopsis root architecture and survival ability in salinity conditions.
Fil: Spies, Fiorella Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Perotti, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Cho, Yuhan. No especifíca;
Fil: Jo, Chang Ig. No especifíca;
Fil: Chang Ig,. University of Missouri; Estados Unidos
Fil: Chan, Raquel Lía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
description Adaptation to soil is a well-regulated process vital for plant life. AtHB23 is a homeodomain-leucine zipper I transcription factor (TF), previously revealed as crucial for plant survival in front of salinity conditions. We wondered whether this TF has partners to achieve this essential function. A TF cDNA library screening, Y2H, BiFC, and CoIP assays were complemented with expression analyses and phenotypic characterizations of silenced, mutant, overexpressor, and crossed plants in normal and salinity conditions. We revealed that AtHB23, AtPHL1, and AtMYB68 interact with each other, modulating root development and salinity response. The encoding genes coexpress in specific root tissues and developmental stages. In normal conditions, amiR68 silenced plants have less initiated roots, the opposite phenotype to that showed by amiR23 ones. AtMYB68 and AtPHL1 play contrary roles in lateral root elongation. Under salinity, where AtHB23 plays a crucial positive function, AtMYB68 cooperates with it, whereas AtPHL1 obstructs its action impacting survival ability and supporting the complex interaction between AtHB23, AtPHL1, and AtMYB68 in the primary and lateral roots. The root adaptation capability was associated with the amyloplast state. We identified new molecular players that through a complex relationship determine Arabidopsis root architecture and survival ability in salinity conditions.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/241586
Spies, Fiorella Paola; Perotti, María Florencia; Cho, Yuhan; Jo, Chang Ig; Chang Ig,; et al.; A complex tissue‐specific interplay between the Arabidopsis transcription factors AtMYB68 , AtHB23 , and AtPHL1 modulates primary and lateral root development and adaptation to salinity; Wiley Blackwell Publishing, Inc; Plant Journal; 115; 4; 5-2023; 952-966
0960-7412
CONICET Digital
CONICET
url http://hdl.handle.net/11336/241586
identifier_str_mv Spies, Fiorella Paola; Perotti, María Florencia; Cho, Yuhan; Jo, Chang Ig; Chang Ig,; et al.; A complex tissue‐specific interplay between the Arabidopsis transcription factors AtMYB68 , AtHB23 , and AtPHL1 modulates primary and lateral root development and adaptation to salinity; Wiley Blackwell Publishing, Inc; Plant Journal; 115; 4; 5-2023; 952-966
0960-7412
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/tpj.16273
info:eu-repo/semantics/altIdentifier/doi/10.1111/tpj.16273
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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