Probiotic fermented milk consumption modulates the allergic process induced by ovoalbumin in mice

Autores
Velez, Eva Maria del Mar; Maldonado Galdeano, María Carolina; Carmuega, Esteban; Weill, Ricardo; Bibas Bonet, María Eugenia; Perdigon, Gabriela del Valle
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Orally administered probiotic micro-organisms are able to regulate the exacerbated immune response during the antigenic sensitisationprocess. The aim of the present study was to evaluate the potential efficacy of probiotic fermented milk (PFM) in preventing or treatingallergy in an experimental model, and to investigate its underlying mechanisms. Ovoalbumin (OVA)-sensitised BALB/c mice were fed withPFM before the sensitisation procedure or fed continuously with PFM. At 7 and 15 d post-sensitisation, anti-OVA-specific IgE, IgG, IgG1 and15 Q5 IgG2a concentrations were measured in the serum and broncho-alveolar lavage fluid (BALF). Concentrations of interferon-g (IFN-g), IL-4, IL-10 and total S-IgA were measured in the supernatants of macerated lungs or in the BALF. The levels of IgAþ, CD4þ and CD8þ Q6 T lymphocytes and F4/80þ cells were measured in the lungs by immunofluorescence. Inducible CD4þ/CD25/Foxp3þ regulatory T (Treg) cells were evaluated in the lungs. PFM shifted the T helper (Th)2 profile response towards a Th1 response that led to the production of IgG instead of IgE, with increasing levels of IL-10 and IFN-g that play an important role in immunomodulation exerted by PFM admin- istration in sensitised mice. Anti-OVA-specific IgE levels were significantly decreased; however, there was no modification in the levels ofanti-OVA-specific IgG and total S-IgA. PFM did not influence Treg cells in treated mice. Consumption of PFM could be a promising strategy in the amelioration of airway allergies, considering that the effect is mediated by the production of IgG through the activation of Th1instead of the direct activation of Th2 cells to produce IgE.
Fil: Velez, Eva Maria del Mar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Carmuega, Esteban. Centro de Estudios Nutricionales Infantiles; Argentina
Fil: Weill, Ricardo. DANONE; Argentina
Fil: Bibas Bonet, María Eugenia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Materia
Allergy Model
Interferon-Γ
Ovoalbumin
Probiotic Fermented Milk
T Helper 1/T Helper 2 Balance
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/28426
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/28426
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Probiotic fermented milk consumption modulates the allergic process induced by ovoalbumin in miceVelez, Eva Maria del MarMaldonado Galdeano, María CarolinaCarmuega, EstebanWeill, RicardoBibas Bonet, María EugeniaPerdigon, Gabriela del ValleAllergy ModelInterferon-ΓOvoalbuminProbiotic Fermented MilkT Helper 1/T Helper 2 Balancehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Orally administered probiotic micro-organisms are able to regulate the exacerbated immune response during the antigenic sensitisationprocess. The aim of the present study was to evaluate the potential efficacy of probiotic fermented milk (PFM) in preventing or treatingallergy in an experimental model, and to investigate its underlying mechanisms. Ovoalbumin (OVA)-sensitised BALB/c mice were fed withPFM before the sensitisation procedure or fed continuously with PFM. At 7 and 15 d post-sensitisation, anti-OVA-specific IgE, IgG, IgG1 and15 Q5 IgG2a concentrations were measured in the serum and broncho-alveolar lavage fluid (BALF). Concentrations of interferon-g (IFN-g), IL-4, IL-10 and total S-IgA were measured in the supernatants of macerated lungs or in the BALF. The levels of IgAþ, CD4þ and CD8þ Q6 T lymphocytes and F4/80þ cells were measured in the lungs by immunofluorescence. Inducible CD4þ/CD25/Foxp3þ regulatory T (Treg) cells were evaluated in the lungs. PFM shifted the T helper (Th)2 profile response towards a Th1 response that led to the production of IgG instead of IgE, with increasing levels of IL-10 and IFN-g that play an important role in immunomodulation exerted by PFM admin- istration in sensitised mice. Anti-OVA-specific IgE levels were significantly decreased; however, there was no modification in the levels ofanti-OVA-specific IgG and total S-IgA. PFM did not influence Treg cells in treated mice. Consumption of PFM could be a promising strategy in the amelioration of airway allergies, considering that the effect is mediated by the production of IgG through the activation of Th1instead of the direct activation of Th2 cells to produce IgE.Fil: Velez, Eva Maria del Mar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaFil: Carmuega, Esteban. Centro de Estudios Nutricionales Infantiles; ArgentinaFil: Weill, Ricardo. DANONE; ArgentinaFil: Bibas Bonet, María Eugenia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaFil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaCambridge University Press2015-07-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/28426Velez, Eva Maria del Mar; Maldonado Galdeano, María Carolina; Carmuega, Esteban; Weill, Ricardo; Bibas Bonet, María Eugenia; et al.; Probiotic fermented milk consumption modulates the allergic process induced by ovoalbumin in mice; Cambridge University Press; British Journal of Nutrition; 114; 4; 16-7-2015; 566-5760007-11451475-2662CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0007114515001981info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/probiotic-fermented-milk-consumption-modulates-the-allergic-process-induced-by-ovoalbumin-in-mice/B82D93268C1D6E03C079C3DF978D030Cinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:18Zoai:ri.conicet.gov.ar:11336/28426instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:19.198CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Probiotic fermented milk consumption modulates the allergic process induced by ovoalbumin in mice
title Probiotic fermented milk consumption modulates the allergic process induced by ovoalbumin in mice
spellingShingle Probiotic fermented milk consumption modulates the allergic process induced by ovoalbumin in mice
Velez, Eva Maria del Mar
Allergy Model
Interferon-Γ
Ovoalbumin
Probiotic Fermented Milk
T Helper 1/T Helper 2 Balance
title_short Probiotic fermented milk consumption modulates the allergic process induced by ovoalbumin in mice
title_full Probiotic fermented milk consumption modulates the allergic process induced by ovoalbumin in mice
title_fullStr Probiotic fermented milk consumption modulates the allergic process induced by ovoalbumin in mice
title_full_unstemmed Probiotic fermented milk consumption modulates the allergic process induced by ovoalbumin in mice
title_sort Probiotic fermented milk consumption modulates the allergic process induced by ovoalbumin in mice
dc.creator.none.fl_str_mv Velez, Eva Maria del Mar
Maldonado Galdeano, María Carolina
Carmuega, Esteban
Weill, Ricardo
Bibas Bonet, María Eugenia
Perdigon, Gabriela del Valle
author Velez, Eva Maria del Mar
author_facet Velez, Eva Maria del Mar
Maldonado Galdeano, María Carolina
Carmuega, Esteban
Weill, Ricardo
Bibas Bonet, María Eugenia
Perdigon, Gabriela del Valle
author_role author
author2 Maldonado Galdeano, María Carolina
Carmuega, Esteban
Weill, Ricardo
Bibas Bonet, María Eugenia
Perdigon, Gabriela del Valle
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Allergy Model
Interferon-Γ
Ovoalbumin
Probiotic Fermented Milk
T Helper 1/T Helper 2 Balance
topic Allergy Model
Interferon-Γ
Ovoalbumin
Probiotic Fermented Milk
T Helper 1/T Helper 2 Balance
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Orally administered probiotic micro-organisms are able to regulate the exacerbated immune response during the antigenic sensitisationprocess. The aim of the present study was to evaluate the potential efficacy of probiotic fermented milk (PFM) in preventing or treatingallergy in an experimental model, and to investigate its underlying mechanisms. Ovoalbumin (OVA)-sensitised BALB/c mice were fed withPFM before the sensitisation procedure or fed continuously with PFM. At 7 and 15 d post-sensitisation, anti-OVA-specific IgE, IgG, IgG1 and15 Q5 IgG2a concentrations were measured in the serum and broncho-alveolar lavage fluid (BALF). Concentrations of interferon-g (IFN-g), IL-4, IL-10 and total S-IgA were measured in the supernatants of macerated lungs or in the BALF. The levels of IgAþ, CD4þ and CD8þ Q6 T lymphocytes and F4/80þ cells were measured in the lungs by immunofluorescence. Inducible CD4þ/CD25/Foxp3þ regulatory T (Treg) cells were evaluated in the lungs. PFM shifted the T helper (Th)2 profile response towards a Th1 response that led to the production of IgG instead of IgE, with increasing levels of IL-10 and IFN-g that play an important role in immunomodulation exerted by PFM admin- istration in sensitised mice. Anti-OVA-specific IgE levels were significantly decreased; however, there was no modification in the levels ofanti-OVA-specific IgG and total S-IgA. PFM did not influence Treg cells in treated mice. Consumption of PFM could be a promising strategy in the amelioration of airway allergies, considering that the effect is mediated by the production of IgG through the activation of Th1instead of the direct activation of Th2 cells to produce IgE.
Fil: Velez, Eva Maria del Mar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Carmuega, Esteban. Centro de Estudios Nutricionales Infantiles; Argentina
Fil: Weill, Ricardo. DANONE; Argentina
Fil: Bibas Bonet, María Eugenia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
description Orally administered probiotic micro-organisms are able to regulate the exacerbated immune response during the antigenic sensitisationprocess. The aim of the present study was to evaluate the potential efficacy of probiotic fermented milk (PFM) in preventing or treatingallergy in an experimental model, and to investigate its underlying mechanisms. Ovoalbumin (OVA)-sensitised BALB/c mice were fed withPFM before the sensitisation procedure or fed continuously with PFM. At 7 and 15 d post-sensitisation, anti-OVA-specific IgE, IgG, IgG1 and15 Q5 IgG2a concentrations were measured in the serum and broncho-alveolar lavage fluid (BALF). Concentrations of interferon-g (IFN-g), IL-4, IL-10 and total S-IgA were measured in the supernatants of macerated lungs or in the BALF. The levels of IgAþ, CD4þ and CD8þ Q6 T lymphocytes and F4/80þ cells were measured in the lungs by immunofluorescence. Inducible CD4þ/CD25/Foxp3þ regulatory T (Treg) cells were evaluated in the lungs. PFM shifted the T helper (Th)2 profile response towards a Th1 response that led to the production of IgG instead of IgE, with increasing levels of IL-10 and IFN-g that play an important role in immunomodulation exerted by PFM admin- istration in sensitised mice. Anti-OVA-specific IgE levels were significantly decreased; however, there was no modification in the levels ofanti-OVA-specific IgG and total S-IgA. PFM did not influence Treg cells in treated mice. Consumption of PFM could be a promising strategy in the amelioration of airway allergies, considering that the effect is mediated by the production of IgG through the activation of Th1instead of the direct activation of Th2 cells to produce IgE.
publishDate 2015
dc.date.none.fl_str_mv 2015-07-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/28426
Velez, Eva Maria del Mar; Maldonado Galdeano, María Carolina; Carmuega, Esteban; Weill, Ricardo; Bibas Bonet, María Eugenia; et al.; Probiotic fermented milk consumption modulates the allergic process induced by ovoalbumin in mice; Cambridge University Press; British Journal of Nutrition; 114; 4; 16-7-2015; 566-576
0007-1145
1475-2662
CONICET Digital
CONICET
url http://hdl.handle.net/11336/28426
identifier_str_mv Velez, Eva Maria del Mar; Maldonado Galdeano, María Carolina; Carmuega, Esteban; Weill, Ricardo; Bibas Bonet, María Eugenia; et al.; Probiotic fermented milk consumption modulates the allergic process induced by ovoalbumin in mice; Cambridge University Press; British Journal of Nutrition; 114; 4; 16-7-2015; 566-576
0007-1145
1475-2662
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1017/S0007114515001981
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/probiotic-fermented-milk-consumption-modulates-the-allergic-process-induced-by-ovoalbumin-in-mice/B82D93268C1D6E03C079C3DF978D030C
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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