Sex-dependent impact of Roundup on the rat gut microbiome

Autores
Lozano, Verónica Laura; Defarge, Nicolas; Rocque, Louis-Marie; Mesnage, Robin; Hennequin, Didier; Cassier, Renaud; de Vendômois, Joël Spiroux; Panoff, Jean-Michel; Séralini, Gilles Eric; Amiel, Caroline
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A growing body of research suggests that dysbiosis of the gut microbiota induced by environmental pollutants, such as pesticides, could have a role in the development of metabolic disorders. We have examined the long-term effects of 3 doses of the Roundup(R) herbicide (made of glyphosate and formulants) on the gut microbiota in male and female Sprague-Dawley rats. A total of 141 bacteria families were identified by a 16S sequencing analysis approach. An OPLS-DA analysis revealed an increased Bacteroidetes family S24-7 and a decreased Lactobacillaceae in 8 out of the 9 females treated with 3 different doses of R (n = 3, for each dose). These effects were confirmed by repetitive sequence-based PCR fingerprinting showing a clustering of treated females. A culture-based method showed that R had a direct effect on rat gut microbiota. Cultivable species showed different sensitivities to R, including the presence of a high tolerant or resistant strain identified as Escherichia coli by 16S rRNA sequencing. The high tolerance of this E. Coli strain was explained by the absence of the EPSPS gene (coding glyphosate target enzyme) as shown by DNA amplification. Overall, these gut microbiome disturbances showed a substantial overlap with those associated with liver dysfunction in other studies. In conclusion, we revealed that an environmental concentration of R (0.1 ppb) and other two concentrations (400 ppm and 5,000 ppm) have a sex-dependent impact on rat gut microbiome composition and thus warrants further investigation.
Fil: Lozano, Verónica Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universite de Caen Basse Normandie; Francia
Fil: Defarge, Nicolas. Universite de Caen Basse Normandie; Francia. CRIIGEN; Francia
Fil: Rocque, Louis-Marie. CRIIGEN; Francia. AdGène Laboratoire; Francia
Fil: Mesnage, Robin. AdGène Laboratoire; Francia. Guy's Hospital; Reino Unido
Fil: Hennequin, Didier. Universite de Caen Basse Normandie; Francia
Fil: Cassier, Renaud. AdGène Laboratoire; Francia
Fil: de Vendômois, Joël Spiroux. CRIIGEN; Francia
Fil: Panoff, Jean-Michel. CRIIGEN; Francia. Universite de Caen Basse Normandie; Francia
Fil: Séralini, Gilles Eric. CRIIGEN; Francia. Universite de Caen Basse Normandie; Francia
Fil: Amiel, Caroline. CRIIGEN; Francia. Universite de Caen Basse Normandie; Francia
Materia
GLYPHOSATE
GUT MICROBIOME
PESTICIDES
ROUNDUP
TOXICITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/65120
Acceder
id CONICETDig_526046327d0074e728f9835ced2dc1ee
oai_identifier_str oai:ri.conicet.gov.ar:11336/65120
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sex-dependent impact of Roundup on the rat gut microbiomeLozano, Verónica LauraDefarge, NicolasRocque, Louis-MarieMesnage, RobinHennequin, DidierCassier, Renaudde Vendômois, Joël SpirouxPanoff, Jean-MichelSéralini, Gilles EricAmiel, CarolineGLYPHOSATEGUT MICROBIOMEPESTICIDESROUNDUPTOXICITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A growing body of research suggests that dysbiosis of the gut microbiota induced by environmental pollutants, such as pesticides, could have a role in the development of metabolic disorders. We have examined the long-term effects of 3 doses of the Roundup(R) herbicide (made of glyphosate and formulants) on the gut microbiota in male and female Sprague-Dawley rats. A total of 141 bacteria families were identified by a 16S sequencing analysis approach. An OPLS-DA analysis revealed an increased Bacteroidetes family S24-7 and a decreased Lactobacillaceae in 8 out of the 9 females treated with 3 different doses of R (n = 3, for each dose). These effects were confirmed by repetitive sequence-based PCR fingerprinting showing a clustering of treated females. A culture-based method showed that R had a direct effect on rat gut microbiota. Cultivable species showed different sensitivities to R, including the presence of a high tolerant or resistant strain identified as Escherichia coli by 16S rRNA sequencing. The high tolerance of this E. Coli strain was explained by the absence of the EPSPS gene (coding glyphosate target enzyme) as shown by DNA amplification. Overall, these gut microbiome disturbances showed a substantial overlap with those associated with liver dysfunction in other studies. In conclusion, we revealed that an environmental concentration of R (0.1 ppb) and other two concentrations (400 ppm and 5,000 ppm) have a sex-dependent impact on rat gut microbiome composition and thus warrants further investigation.Fil: Lozano, Verónica Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universite de Caen Basse Normandie; FranciaFil: Defarge, Nicolas. Universite de Caen Basse Normandie; Francia. CRIIGEN; FranciaFil: Rocque, Louis-Marie. CRIIGEN; Francia. AdGène Laboratoire; FranciaFil: Mesnage, Robin. AdGène Laboratoire; Francia. Guy's Hospital; Reino UnidoFil: Hennequin, Didier. Universite de Caen Basse Normandie; FranciaFil: Cassier, Renaud. AdGène Laboratoire; FranciaFil: de Vendômois, Joël Spiroux. CRIIGEN; FranciaFil: Panoff, Jean-Michel. CRIIGEN; Francia. Universite de Caen Basse Normandie; FranciaFil: Séralini, Gilles Eric. CRIIGEN; Francia. Universite de Caen Basse Normandie; FranciaFil: Amiel, Caroline. CRIIGEN; Francia. Universite de Caen Basse Normandie; FranciaElsevier Inc.2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/65120Lozano, Verónica Laura; Defarge, Nicolas; Rocque, Louis-Marie; Mesnage, Robin; Hennequin, Didier; et al.; Sex-dependent impact of Roundup on the rat gut microbiome; Elsevier Inc.; Toxicology Reports; 5; 12-2017; 96-1072214-7500CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S2214750017301129info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxrep.2017.12.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:41:22Zoai:ri.conicet.gov.ar:11336/65120instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:41:22.794CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sex-dependent impact of Roundup on the rat gut microbiome
title Sex-dependent impact of Roundup on the rat gut microbiome
spellingShingle Sex-dependent impact of Roundup on the rat gut microbiome
Lozano, Verónica Laura
GLYPHOSATE
GUT MICROBIOME
PESTICIDES
ROUNDUP
TOXICITY
title_short Sex-dependent impact of Roundup on the rat gut microbiome
title_full Sex-dependent impact of Roundup on the rat gut microbiome
title_fullStr Sex-dependent impact of Roundup on the rat gut microbiome
title_full_unstemmed Sex-dependent impact of Roundup on the rat gut microbiome
title_sort Sex-dependent impact of Roundup on the rat gut microbiome
dc.creator.none.fl_str_mv Lozano, Verónica Laura
Defarge, Nicolas
Rocque, Louis-Marie
Mesnage, Robin
Hennequin, Didier
Cassier, Renaud
de Vendômois, Joël Spiroux
Panoff, Jean-Michel
Séralini, Gilles Eric
Amiel, Caroline
author Lozano, Verónica Laura
author_facet Lozano, Verónica Laura
Defarge, Nicolas
Rocque, Louis-Marie
Mesnage, Robin
Hennequin, Didier
Cassier, Renaud
de Vendômois, Joël Spiroux
Panoff, Jean-Michel
Séralini, Gilles Eric
Amiel, Caroline
author_role author
author2 Defarge, Nicolas
Rocque, Louis-Marie
Mesnage, Robin
Hennequin, Didier
Cassier, Renaud
de Vendômois, Joël Spiroux
Panoff, Jean-Michel
Séralini, Gilles Eric
Amiel, Caroline
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GLYPHOSATE
GUT MICROBIOME
PESTICIDES
ROUNDUP
TOXICITY
topic GLYPHOSATE
GUT MICROBIOME
PESTICIDES
ROUNDUP
TOXICITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A growing body of research suggests that dysbiosis of the gut microbiota induced by environmental pollutants, such as pesticides, could have a role in the development of metabolic disorders. We have examined the long-term effects of 3 doses of the Roundup(R) herbicide (made of glyphosate and formulants) on the gut microbiota in male and female Sprague-Dawley rats. A total of 141 bacteria families were identified by a 16S sequencing analysis approach. An OPLS-DA analysis revealed an increased Bacteroidetes family S24-7 and a decreased Lactobacillaceae in 8 out of the 9 females treated with 3 different doses of R (n = 3, for each dose). These effects were confirmed by repetitive sequence-based PCR fingerprinting showing a clustering of treated females. A culture-based method showed that R had a direct effect on rat gut microbiota. Cultivable species showed different sensitivities to R, including the presence of a high tolerant or resistant strain identified as Escherichia coli by 16S rRNA sequencing. The high tolerance of this E. Coli strain was explained by the absence of the EPSPS gene (coding glyphosate target enzyme) as shown by DNA amplification. Overall, these gut microbiome disturbances showed a substantial overlap with those associated with liver dysfunction in other studies. In conclusion, we revealed that an environmental concentration of R (0.1 ppb) and other two concentrations (400 ppm and 5,000 ppm) have a sex-dependent impact on rat gut microbiome composition and thus warrants further investigation.
Fil: Lozano, Verónica Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universite de Caen Basse Normandie; Francia
Fil: Defarge, Nicolas. Universite de Caen Basse Normandie; Francia. CRIIGEN; Francia
Fil: Rocque, Louis-Marie. CRIIGEN; Francia. AdGène Laboratoire; Francia
Fil: Mesnage, Robin. AdGène Laboratoire; Francia. Guy's Hospital; Reino Unido
Fil: Hennequin, Didier. Universite de Caen Basse Normandie; Francia
Fil: Cassier, Renaud. AdGène Laboratoire; Francia
Fil: de Vendômois, Joël Spiroux. CRIIGEN; Francia
Fil: Panoff, Jean-Michel. CRIIGEN; Francia. Universite de Caen Basse Normandie; Francia
Fil: Séralini, Gilles Eric. CRIIGEN; Francia. Universite de Caen Basse Normandie; Francia
Fil: Amiel, Caroline. CRIIGEN; Francia. Universite de Caen Basse Normandie; Francia
description A growing body of research suggests that dysbiosis of the gut microbiota induced by environmental pollutants, such as pesticides, could have a role in the development of metabolic disorders. We have examined the long-term effects of 3 doses of the Roundup(R) herbicide (made of glyphosate and formulants) on the gut microbiota in male and female Sprague-Dawley rats. A total of 141 bacteria families were identified by a 16S sequencing analysis approach. An OPLS-DA analysis revealed an increased Bacteroidetes family S24-7 and a decreased Lactobacillaceae in 8 out of the 9 females treated with 3 different doses of R (n = 3, for each dose). These effects were confirmed by repetitive sequence-based PCR fingerprinting showing a clustering of treated females. A culture-based method showed that R had a direct effect on rat gut microbiota. Cultivable species showed different sensitivities to R, including the presence of a high tolerant or resistant strain identified as Escherichia coli by 16S rRNA sequencing. The high tolerance of this E. Coli strain was explained by the absence of the EPSPS gene (coding glyphosate target enzyme) as shown by DNA amplification. Overall, these gut microbiome disturbances showed a substantial overlap with those associated with liver dysfunction in other studies. In conclusion, we revealed that an environmental concentration of R (0.1 ppb) and other two concentrations (400 ppm and 5,000 ppm) have a sex-dependent impact on rat gut microbiome composition and thus warrants further investigation.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/65120
Lozano, Verónica Laura; Defarge, Nicolas; Rocque, Louis-Marie; Mesnage, Robin; Hennequin, Didier; et al.; Sex-dependent impact of Roundup on the rat gut microbiome; Elsevier Inc.; Toxicology Reports; 5; 12-2017; 96-107
2214-7500
CONICET Digital
CONICET
url http://hdl.handle.net/11336/65120
identifier_str_mv Lozano, Verónica Laura; Defarge, Nicolas; Rocque, Louis-Marie; Mesnage, Robin; Hennequin, Didier; et al.; Sex-dependent impact of Roundup on the rat gut microbiome; Elsevier Inc.; Toxicology Reports; 5; 12-2017; 96-107
2214-7500
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S2214750017301129
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxrep.2017.12.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Inc.
publisher.none.fl_str_mv Elsevier Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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