Sex-dependent impact of Roundup on the rat gut microbiome
- Autores
- Lozano, Verónica Laura; Defarge, Nicolas; Rocque, Louis-Marie; Mesnage, Robin; Hennequin, Didier; Cassier, Renaud; de Vendômois, Joël Spiroux; Panoff, Jean-Michel; Séralini, Gilles Eric; Amiel, Caroline
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A growing body of research suggests that dysbiosis of the gut microbiota induced by environmental pollutants, such as pesticides, could have a role in the development of metabolic disorders. We have examined the long-term effects of 3 doses of the Roundup(R) herbicide (made of glyphosate and formulants) on the gut microbiota in male and female Sprague-Dawley rats. A total of 141 bacteria families were identified by a 16S sequencing analysis approach. An OPLS-DA analysis revealed an increased Bacteroidetes family S24-7 and a decreased Lactobacillaceae in 8 out of the 9 females treated with 3 different doses of R (n = 3, for each dose). These effects were confirmed by repetitive sequence-based PCR fingerprinting showing a clustering of treated females. A culture-based method showed that R had a direct effect on rat gut microbiota. Cultivable species showed different sensitivities to R, including the presence of a high tolerant or resistant strain identified as Escherichia coli by 16S rRNA sequencing. The high tolerance of this E. Coli strain was explained by the absence of the EPSPS gene (coding glyphosate target enzyme) as shown by DNA amplification. Overall, these gut microbiome disturbances showed a substantial overlap with those associated with liver dysfunction in other studies. In conclusion, we revealed that an environmental concentration of R (0.1 ppb) and other two concentrations (400 ppm and 5,000 ppm) have a sex-dependent impact on rat gut microbiome composition and thus warrants further investigation.
Fil: Lozano, Verónica Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universite de Caen Basse Normandie; Francia
Fil: Defarge, Nicolas. Universite de Caen Basse Normandie; Francia. CRIIGEN; Francia
Fil: Rocque, Louis-Marie. CRIIGEN; Francia. AdGène Laboratoire; Francia
Fil: Mesnage, Robin. AdGène Laboratoire; Francia. Guy's Hospital; Reino Unido
Fil: Hennequin, Didier. Universite de Caen Basse Normandie; Francia
Fil: Cassier, Renaud. AdGène Laboratoire; Francia
Fil: de Vendômois, Joël Spiroux. CRIIGEN; Francia
Fil: Panoff, Jean-Michel. CRIIGEN; Francia. Universite de Caen Basse Normandie; Francia
Fil: Séralini, Gilles Eric. CRIIGEN; Francia. Universite de Caen Basse Normandie; Francia
Fil: Amiel, Caroline. CRIIGEN; Francia. Universite de Caen Basse Normandie; Francia - Materia
-
GLYPHOSATE
GUT MICROBIOME
PESTICIDES
ROUNDUP
TOXICITY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/65120
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
spelling |
Sex-dependent impact of Roundup on the rat gut microbiomeLozano, Verónica LauraDefarge, NicolasRocque, Louis-MarieMesnage, RobinHennequin, DidierCassier, Renaudde Vendômois, Joël SpirouxPanoff, Jean-MichelSéralini, Gilles EricAmiel, CarolineGLYPHOSATEGUT MICROBIOMEPESTICIDESROUNDUPTOXICITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A growing body of research suggests that dysbiosis of the gut microbiota induced by environmental pollutants, such as pesticides, could have a role in the development of metabolic disorders. We have examined the long-term effects of 3 doses of the Roundup(R) herbicide (made of glyphosate and formulants) on the gut microbiota in male and female Sprague-Dawley rats. A total of 141 bacteria families were identified by a 16S sequencing analysis approach. An OPLS-DA analysis revealed an increased Bacteroidetes family S24-7 and a decreased Lactobacillaceae in 8 out of the 9 females treated with 3 different doses of R (n = 3, for each dose). These effects were confirmed by repetitive sequence-based PCR fingerprinting showing a clustering of treated females. A culture-based method showed that R had a direct effect on rat gut microbiota. Cultivable species showed different sensitivities to R, including the presence of a high tolerant or resistant strain identified as Escherichia coli by 16S rRNA sequencing. The high tolerance of this E. Coli strain was explained by the absence of the EPSPS gene (coding glyphosate target enzyme) as shown by DNA amplification. Overall, these gut microbiome disturbances showed a substantial overlap with those associated with liver dysfunction in other studies. In conclusion, we revealed that an environmental concentration of R (0.1 ppb) and other two concentrations (400 ppm and 5,000 ppm) have a sex-dependent impact on rat gut microbiome composition and thus warrants further investigation.Fil: Lozano, Verónica Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universite de Caen Basse Normandie; FranciaFil: Defarge, Nicolas. Universite de Caen Basse Normandie; Francia. CRIIGEN; FranciaFil: Rocque, Louis-Marie. CRIIGEN; Francia. AdGène Laboratoire; FranciaFil: Mesnage, Robin. AdGène Laboratoire; Francia. Guy's Hospital; Reino UnidoFil: Hennequin, Didier. Universite de Caen Basse Normandie; FranciaFil: Cassier, Renaud. AdGène Laboratoire; FranciaFil: de Vendômois, Joël Spiroux. CRIIGEN; FranciaFil: Panoff, Jean-Michel. CRIIGEN; Francia. Universite de Caen Basse Normandie; FranciaFil: Séralini, Gilles Eric. CRIIGEN; Francia. Universite de Caen Basse Normandie; FranciaFil: Amiel, Caroline. CRIIGEN; Francia. Universite de Caen Basse Normandie; FranciaElsevier Inc.2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/65120Lozano, Verónica Laura; Defarge, Nicolas; Rocque, Louis-Marie; Mesnage, Robin; Hennequin, Didier; et al.; Sex-dependent impact of Roundup on the rat gut microbiome; Elsevier Inc.; Toxicology Reports; 5; 12-2017; 96-1072214-7500CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S2214750017301129info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxrep.2017.12.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:41:22Zoai:ri.conicet.gov.ar:11336/65120instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:41:22.794CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Sex-dependent impact of Roundup on the rat gut microbiome |
title |
Sex-dependent impact of Roundup on the rat gut microbiome |
spellingShingle |
Sex-dependent impact of Roundup on the rat gut microbiome Lozano, Verónica Laura GLYPHOSATE GUT MICROBIOME PESTICIDES ROUNDUP TOXICITY |
title_short |
Sex-dependent impact of Roundup on the rat gut microbiome |
title_full |
Sex-dependent impact of Roundup on the rat gut microbiome |
title_fullStr |
Sex-dependent impact of Roundup on the rat gut microbiome |
title_full_unstemmed |
Sex-dependent impact of Roundup on the rat gut microbiome |
title_sort |
Sex-dependent impact of Roundup on the rat gut microbiome |
dc.creator.none.fl_str_mv |
Lozano, Verónica Laura Defarge, Nicolas Rocque, Louis-Marie Mesnage, Robin Hennequin, Didier Cassier, Renaud de Vendômois, Joël Spiroux Panoff, Jean-Michel Séralini, Gilles Eric Amiel, Caroline |
author |
Lozano, Verónica Laura |
author_facet |
Lozano, Verónica Laura Defarge, Nicolas Rocque, Louis-Marie Mesnage, Robin Hennequin, Didier Cassier, Renaud de Vendômois, Joël Spiroux Panoff, Jean-Michel Séralini, Gilles Eric Amiel, Caroline |
author_role |
author |
author2 |
Defarge, Nicolas Rocque, Louis-Marie Mesnage, Robin Hennequin, Didier Cassier, Renaud de Vendômois, Joël Spiroux Panoff, Jean-Michel Séralini, Gilles Eric Amiel, Caroline |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
GLYPHOSATE GUT MICROBIOME PESTICIDES ROUNDUP TOXICITY |
topic |
GLYPHOSATE GUT MICROBIOME PESTICIDES ROUNDUP TOXICITY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
A growing body of research suggests that dysbiosis of the gut microbiota induced by environmental pollutants, such as pesticides, could have a role in the development of metabolic disorders. We have examined the long-term effects of 3 doses of the Roundup(R) herbicide (made of glyphosate and formulants) on the gut microbiota in male and female Sprague-Dawley rats. A total of 141 bacteria families were identified by a 16S sequencing analysis approach. An OPLS-DA analysis revealed an increased Bacteroidetes family S24-7 and a decreased Lactobacillaceae in 8 out of the 9 females treated with 3 different doses of R (n = 3, for each dose). These effects were confirmed by repetitive sequence-based PCR fingerprinting showing a clustering of treated females. A culture-based method showed that R had a direct effect on rat gut microbiota. Cultivable species showed different sensitivities to R, including the presence of a high tolerant or resistant strain identified as Escherichia coli by 16S rRNA sequencing. The high tolerance of this E. Coli strain was explained by the absence of the EPSPS gene (coding glyphosate target enzyme) as shown by DNA amplification. Overall, these gut microbiome disturbances showed a substantial overlap with those associated with liver dysfunction in other studies. In conclusion, we revealed that an environmental concentration of R (0.1 ppb) and other two concentrations (400 ppm and 5,000 ppm) have a sex-dependent impact on rat gut microbiome composition and thus warrants further investigation. Fil: Lozano, Verónica Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universite de Caen Basse Normandie; Francia Fil: Defarge, Nicolas. Universite de Caen Basse Normandie; Francia. CRIIGEN; Francia Fil: Rocque, Louis-Marie. CRIIGEN; Francia. AdGène Laboratoire; Francia Fil: Mesnage, Robin. AdGène Laboratoire; Francia. Guy's Hospital; Reino Unido Fil: Hennequin, Didier. Universite de Caen Basse Normandie; Francia Fil: Cassier, Renaud. AdGène Laboratoire; Francia Fil: de Vendômois, Joël Spiroux. CRIIGEN; Francia Fil: Panoff, Jean-Michel. CRIIGEN; Francia. Universite de Caen Basse Normandie; Francia Fil: Séralini, Gilles Eric. CRIIGEN; Francia. Universite de Caen Basse Normandie; Francia Fil: Amiel, Caroline. CRIIGEN; Francia. Universite de Caen Basse Normandie; Francia |
description |
A growing body of research suggests that dysbiosis of the gut microbiota induced by environmental pollutants, such as pesticides, could have a role in the development of metabolic disorders. We have examined the long-term effects of 3 doses of the Roundup(R) herbicide (made of glyphosate and formulants) on the gut microbiota in male and female Sprague-Dawley rats. A total of 141 bacteria families were identified by a 16S sequencing analysis approach. An OPLS-DA analysis revealed an increased Bacteroidetes family S24-7 and a decreased Lactobacillaceae in 8 out of the 9 females treated with 3 different doses of R (n = 3, for each dose). These effects were confirmed by repetitive sequence-based PCR fingerprinting showing a clustering of treated females. A culture-based method showed that R had a direct effect on rat gut microbiota. Cultivable species showed different sensitivities to R, including the presence of a high tolerant or resistant strain identified as Escherichia coli by 16S rRNA sequencing. The high tolerance of this E. Coli strain was explained by the absence of the EPSPS gene (coding glyphosate target enzyme) as shown by DNA amplification. Overall, these gut microbiome disturbances showed a substantial overlap with those associated with liver dysfunction in other studies. In conclusion, we revealed that an environmental concentration of R (0.1 ppb) and other two concentrations (400 ppm and 5,000 ppm) have a sex-dependent impact on rat gut microbiome composition and thus warrants further investigation. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/65120 Lozano, Verónica Laura; Defarge, Nicolas; Rocque, Louis-Marie; Mesnage, Robin; Hennequin, Didier; et al.; Sex-dependent impact of Roundup on the rat gut microbiome; Elsevier Inc.; Toxicology Reports; 5; 12-2017; 96-107 2214-7500 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/65120 |
identifier_str_mv |
Lozano, Verónica Laura; Defarge, Nicolas; Rocque, Louis-Marie; Mesnage, Robin; Hennequin, Didier; et al.; Sex-dependent impact of Roundup on the rat gut microbiome; Elsevier Inc.; Toxicology Reports; 5; 12-2017; 96-107 2214-7500 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S2214750017301129 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxrep.2017.12.005 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Inc. |
publisher.none.fl_str_mv |
Elsevier Inc. |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.001348 |