Are Stress-Related Hormones Involved in the Temperature-Dependent Sex Determination of the Broad-Snouted Caiman?

Autores
Iungman, Josefina Luciana; Somoza, Gustavo Manuel; Piña, Carlos Ignacio
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In some reptiles, gonadal outcome is regulated by temperature during a critical period of the embryonic development. Gonadal steroid hormones are seen as effectors of the gonadal differentiation process. Recently, stress and glucocorticoids (GCs), stress-related hormones in vertebrates, have been considered as potential modulators of the sex determination process in some vertebrates that present temperature-dependent sex determination (TSD). In reptiles, corticosterone is the main GC produced, and its administration to eggs causes a bias in sex ratio in some lizards. In this context, we aim at assessing whether dexamethasone (Dex), a potent synthetic glucocorticoid, can modify the sex ratio in Caiman latirostris, a species with strong TSD. As a first step, we incubated embryos at masculinizing temperatures (33°C; 100% males). Different doses of Dex were topically applied to the eggshell at stage 20, prior to gonadal differentiation. We assessed embryonic development at stages 22 and 25 and evaluated some physiological and morphological hatchling traits. Embryonic mortality was not affected by dexamethasone manipulation. No effects of Dex on sex ratio were found and all animals analyzed histologically possessed testes. However, older embryos and hatchlings from Dex treated eggs were heavier, larger, and hatched earlier than control individuals. Our results do not account for Dex involvement in the process of ovarian differentiation, at least under a strong masculinizing temperature. Nevertheless, they suggest that Dex might accelerate embryo development by enhancing intermediate metabolism and/or by stimulating growth hormone secretion.
Fil: Iungman, Josefina Luciana. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Piña, Carlos Ignacio. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); Argentina
Materia
Caiman Latirostris
Dexamethasone
Embryo
Glucocorticoid
Gonad Development
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/78357
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Are Stress-Related Hormones Involved in the Temperature-Dependent Sex Determination of the Broad-Snouted Caiman?Iungman, Josefina LucianaSomoza, Gustavo ManuelPiña, Carlos IgnacioCaiman LatirostrisDexamethasoneEmbryoGlucocorticoidGonad Developmenthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In some reptiles, gonadal outcome is regulated by temperature during a critical period of the embryonic development. Gonadal steroid hormones are seen as effectors of the gonadal differentiation process. Recently, stress and glucocorticoids (GCs), stress-related hormones in vertebrates, have been considered as potential modulators of the sex determination process in some vertebrates that present temperature-dependent sex determination (TSD). In reptiles, corticosterone is the main GC produced, and its administration to eggs causes a bias in sex ratio in some lizards. In this context, we aim at assessing whether dexamethasone (Dex), a potent synthetic glucocorticoid, can modify the sex ratio in Caiman latirostris, a species with strong TSD. As a first step, we incubated embryos at masculinizing temperatures (33°C; 100% males). Different doses of Dex were topically applied to the eggshell at stage 20, prior to gonadal differentiation. We assessed embryonic development at stages 22 and 25 and evaluated some physiological and morphological hatchling traits. Embryonic mortality was not affected by dexamethasone manipulation. No effects of Dex on sex ratio were found and all animals analyzed histologically possessed testes. However, older embryos and hatchlings from Dex treated eggs were heavier, larger, and hatched earlier than control individuals. Our results do not account for Dex involvement in the process of ovarian differentiation, at least under a strong masculinizing temperature. Nevertheless, they suggest that Dex might accelerate embryo development by enhancing intermediate metabolism and/or by stimulating growth hormone secretion.Fil: Iungman, Josefina Luciana. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Piña, Carlos Ignacio. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); ArgentinaSociedade Brasileira de Herpetologia2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/78357Iungman, Josefina Luciana; Somoza, Gustavo Manuel; Piña, Carlos Ignacio; Are Stress-Related Hormones Involved in the Temperature-Dependent Sex Determination of the Broad-Snouted Caiman?; Sociedade Brasileira de Herpetologia; South American Journal of Herpetology; 10; 1; 4-2015; 41-491808-97981982-355XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/doi/abs/10.2994/SAJH-D-14-00027.1info:eu-repo/semantics/altIdentifier/doi/10.2994/SAJH-D-14-00027.1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:43Zoai:ri.conicet.gov.ar:11336/78357instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:43.593CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Are Stress-Related Hormones Involved in the Temperature-Dependent Sex Determination of the Broad-Snouted Caiman?
title Are Stress-Related Hormones Involved in the Temperature-Dependent Sex Determination of the Broad-Snouted Caiman?
spellingShingle Are Stress-Related Hormones Involved in the Temperature-Dependent Sex Determination of the Broad-Snouted Caiman?
Iungman, Josefina Luciana
Caiman Latirostris
Dexamethasone
Embryo
Glucocorticoid
Gonad Development
title_short Are Stress-Related Hormones Involved in the Temperature-Dependent Sex Determination of the Broad-Snouted Caiman?
title_full Are Stress-Related Hormones Involved in the Temperature-Dependent Sex Determination of the Broad-Snouted Caiman?
title_fullStr Are Stress-Related Hormones Involved in the Temperature-Dependent Sex Determination of the Broad-Snouted Caiman?
title_full_unstemmed Are Stress-Related Hormones Involved in the Temperature-Dependent Sex Determination of the Broad-Snouted Caiman?
title_sort Are Stress-Related Hormones Involved in the Temperature-Dependent Sex Determination of the Broad-Snouted Caiman?
dc.creator.none.fl_str_mv Iungman, Josefina Luciana
Somoza, Gustavo Manuel
Piña, Carlos Ignacio
author Iungman, Josefina Luciana
author_facet Iungman, Josefina Luciana
Somoza, Gustavo Manuel
Piña, Carlos Ignacio
author_role author
author2 Somoza, Gustavo Manuel
Piña, Carlos Ignacio
author2_role author
author
dc.subject.none.fl_str_mv Caiman Latirostris
Dexamethasone
Embryo
Glucocorticoid
Gonad Development
topic Caiman Latirostris
Dexamethasone
Embryo
Glucocorticoid
Gonad Development
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In some reptiles, gonadal outcome is regulated by temperature during a critical period of the embryonic development. Gonadal steroid hormones are seen as effectors of the gonadal differentiation process. Recently, stress and glucocorticoids (GCs), stress-related hormones in vertebrates, have been considered as potential modulators of the sex determination process in some vertebrates that present temperature-dependent sex determination (TSD). In reptiles, corticosterone is the main GC produced, and its administration to eggs causes a bias in sex ratio in some lizards. In this context, we aim at assessing whether dexamethasone (Dex), a potent synthetic glucocorticoid, can modify the sex ratio in Caiman latirostris, a species with strong TSD. As a first step, we incubated embryos at masculinizing temperatures (33°C; 100% males). Different doses of Dex were topically applied to the eggshell at stage 20, prior to gonadal differentiation. We assessed embryonic development at stages 22 and 25 and evaluated some physiological and morphological hatchling traits. Embryonic mortality was not affected by dexamethasone manipulation. No effects of Dex on sex ratio were found and all animals analyzed histologically possessed testes. However, older embryos and hatchlings from Dex treated eggs were heavier, larger, and hatched earlier than control individuals. Our results do not account for Dex involvement in the process of ovarian differentiation, at least under a strong masculinizing temperature. Nevertheless, they suggest that Dex might accelerate embryo development by enhancing intermediate metabolism and/or by stimulating growth hormone secretion.
Fil: Iungman, Josefina Luciana. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Piña, Carlos Ignacio. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); Argentina
description In some reptiles, gonadal outcome is regulated by temperature during a critical period of the embryonic development. Gonadal steroid hormones are seen as effectors of the gonadal differentiation process. Recently, stress and glucocorticoids (GCs), stress-related hormones in vertebrates, have been considered as potential modulators of the sex determination process in some vertebrates that present temperature-dependent sex determination (TSD). In reptiles, corticosterone is the main GC produced, and its administration to eggs causes a bias in sex ratio in some lizards. In this context, we aim at assessing whether dexamethasone (Dex), a potent synthetic glucocorticoid, can modify the sex ratio in Caiman latirostris, a species with strong TSD. As a first step, we incubated embryos at masculinizing temperatures (33°C; 100% males). Different doses of Dex were topically applied to the eggshell at stage 20, prior to gonadal differentiation. We assessed embryonic development at stages 22 and 25 and evaluated some physiological and morphological hatchling traits. Embryonic mortality was not affected by dexamethasone manipulation. No effects of Dex on sex ratio were found and all animals analyzed histologically possessed testes. However, older embryos and hatchlings from Dex treated eggs were heavier, larger, and hatched earlier than control individuals. Our results do not account for Dex involvement in the process of ovarian differentiation, at least under a strong masculinizing temperature. Nevertheless, they suggest that Dex might accelerate embryo development by enhancing intermediate metabolism and/or by stimulating growth hormone secretion.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/78357
Iungman, Josefina Luciana; Somoza, Gustavo Manuel; Piña, Carlos Ignacio; Are Stress-Related Hormones Involved in the Temperature-Dependent Sex Determination of the Broad-Snouted Caiman?; Sociedade Brasileira de Herpetologia; South American Journal of Herpetology; 10; 1; 4-2015; 41-49
1808-9798
1982-355X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/78357
identifier_str_mv Iungman, Josefina Luciana; Somoza, Gustavo Manuel; Piña, Carlos Ignacio; Are Stress-Related Hormones Involved in the Temperature-Dependent Sex Determination of the Broad-Snouted Caiman?; Sociedade Brasileira de Herpetologia; South American Journal of Herpetology; 10; 1; 4-2015; 41-49
1808-9798
1982-355X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.2994/SAJH-D-14-00027.1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Herpetologia
publisher.none.fl_str_mv Sociedade Brasileira de Herpetologia
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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