Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris

Autores
Jacobs, Melisa; Salinas, Silvina Rosa; Bianco, María Isabel; Ielpi, Luis
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
GumB is a predicted outer membrane lipoprotein that is involved in the synthesis and/or secretion of xanthan gum. This exopolysaccharide, produced by Xanthomonas campestris, is valuable in industry because of its important rheological properties. Solution of the GumB structure will provide insight into the polymerization and/or secretion mechanisms of xanthan gum. GumB was overexpressed and purified and diffraction-quality crystals of native GumB were obtained. A complete data set was collected to 2.54 Å resolution with an R(p.i.m.) of 0.034. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 84.4, b = 90.5, c = 120.7 Å.
Fil: Jacobs, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Salinas, Silvina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Bianco, María Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Ielpi, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Materia
xanthan gum
exopolysaccharides
outer membrane proteins
lipoproteins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/273878
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestrisJacobs, MelisaSalinas, Silvina RosaBianco, María IsabelIelpi, Luisxanthan gumexopolysaccharidesouter membrane proteinslipoproteinshttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1GumB is a predicted outer membrane lipoprotein that is involved in the synthesis and/or secretion of xanthan gum. This exopolysaccharide, produced by Xanthomonas campestris, is valuable in industry because of its important rheological properties. Solution of the GumB structure will provide insight into the polymerization and/or secretion mechanisms of xanthan gum. GumB was overexpressed and purified and diffraction-quality crystals of native GumB were obtained. A complete data set was collected to 2.54 Å resolution with an R(p.i.m.) of 0.034. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 84.4, b = 90.5, c = 120.7 Å.Fil: Jacobs, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Salinas, Silvina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Bianco, María Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Ielpi, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaWiley Blackwell Publishing, Inc2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/273878Jacobs, Melisa; Salinas, Silvina Rosa; Bianco, María Isabel; Ielpi, Luis; Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris; Wiley Blackwell Publishing, Inc; Acta Crystallographica Section F-structural Biology And Crystallization Communications; 68; 10; 9-2012; 1255-12581744-3091CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.iucr.org/paper?S1744309112036597info:eu-repo/semantics/altIdentifier/doi/10.1107/S1744309112036597info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:39:20Zoai:ri.conicet.gov.ar:11336/273878instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:39:21.173CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris
title Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris
spellingShingle Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris
Jacobs, Melisa
xanthan gum
exopolysaccharides
outer membrane proteins
lipoproteins
title_short Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris
title_full Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris
title_fullStr Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris
title_full_unstemmed Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris
title_sort Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris
dc.creator.none.fl_str_mv Jacobs, Melisa
Salinas, Silvina Rosa
Bianco, María Isabel
Ielpi, Luis
author Jacobs, Melisa
author_facet Jacobs, Melisa
Salinas, Silvina Rosa
Bianco, María Isabel
Ielpi, Luis
author_role author
author2 Salinas, Silvina Rosa
Bianco, María Isabel
Ielpi, Luis
author2_role author
author
author
dc.subject.none.fl_str_mv xanthan gum
exopolysaccharides
outer membrane proteins
lipoproteins
topic xanthan gum
exopolysaccharides
outer membrane proteins
lipoproteins
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv GumB is a predicted outer membrane lipoprotein that is involved in the synthesis and/or secretion of xanthan gum. This exopolysaccharide, produced by Xanthomonas campestris, is valuable in industry because of its important rheological properties. Solution of the GumB structure will provide insight into the polymerization and/or secretion mechanisms of xanthan gum. GumB was overexpressed and purified and diffraction-quality crystals of native GumB were obtained. A complete data set was collected to 2.54 Å resolution with an R(p.i.m.) of 0.034. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 84.4, b = 90.5, c = 120.7 Å.
Fil: Jacobs, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Salinas, Silvina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Bianco, María Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Ielpi, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
description GumB is a predicted outer membrane lipoprotein that is involved in the synthesis and/or secretion of xanthan gum. This exopolysaccharide, produced by Xanthomonas campestris, is valuable in industry because of its important rheological properties. Solution of the GumB structure will provide insight into the polymerization and/or secretion mechanisms of xanthan gum. GumB was overexpressed and purified and diffraction-quality crystals of native GumB were obtained. A complete data set was collected to 2.54 Å resolution with an R(p.i.m.) of 0.034. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 84.4, b = 90.5, c = 120.7 Å.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/273878
Jacobs, Melisa; Salinas, Silvina Rosa; Bianco, María Isabel; Ielpi, Luis; Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris; Wiley Blackwell Publishing, Inc; Acta Crystallographica Section F-structural Biology And Crystallization Communications; 68; 10; 9-2012; 1255-1258
1744-3091
CONICET Digital
CONICET
url http://hdl.handle.net/11336/273878
identifier_str_mv Jacobs, Melisa; Salinas, Silvina Rosa; Bianco, María Isabel; Ielpi, Luis; Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris; Wiley Blackwell Publishing, Inc; Acta Crystallographica Section F-structural Biology And Crystallization Communications; 68; 10; 9-2012; 1255-1258
1744-3091
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.iucr.org/paper?S1744309112036597
info:eu-repo/semantics/altIdentifier/doi/10.1107/S1744309112036597
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.087074

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