Small molecule-mediated stabilization of vesicle-associated helical Alpha-synuclein inhibits patogenic misfolding and aggregation
- Autores
- Fonseca Ornelas, Luis; Eisbach, Sybille E.; Paulat, Maria; Giller, Karin; Fernandez, Claudio Oscar; Outeiro, Tiago F; Becker, Stefan; Zweckstetter, Markus
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- α-synuclein is an abundant presynaptic protein that is important for regulation of synaptic vesicle trafficking, and whose misfolding plays a key role in Parkinson’s disease. While α-synuclein is disordered in solution, it folds into a helical conformation when bound to synaptic vesicles. Stabilization of helical, folded α-synuclein might therefore interfere with α-synuclein-induced neurotoxicity. Here we show that several small molecules, which delay aggregation of α-synuclein in solution, including the Parkinson’s disease drug selegiline, fail to interfere with misfolding of vesicle-bound α-synuclein. In contrast, the porphyrin phtalocyanine tetrasulfonate directly binds to vesicle-bound α-synuclein, stabilizes its helical conformation and thereby delays pathogenic misfolding and aggregation. Our study suggests that small-molecule-mediated stabilization of helical vesicle-bound α-synuclein opens new possibilities to target Parkinson’s disease and related synucleinopathies
Fil: Fonseca Ornelas, Luis. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Eisbach, Sybille E.. University Medicine; Alemania
Fil: Paulat, Maria. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Giller, Karin. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Fernandez, Claudio Oscar. Universidad Nacional de Rosario; Argentina. Instituto de Investigaciones para el Descubrimiento de Farmacos de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Outeiro, Tiago F. University Medicine; Alemania. University Medical Center; Alemania
Fil: Becker, Stefan. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Zweckstetter, Markus. Max Planck Institute for Biophysical Chemistry; Alemania. University Medical Center; Alemania. German Center for Neurodegenerative Diseases; Alemania - Materia
-
amiloide
inhibicion
sinucleina
PcTS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/29704
Ver los metadatos del registro completo
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Small molecule-mediated stabilization of vesicle-associated helical Alpha-synuclein inhibits patogenic misfolding and aggregationFonseca Ornelas, LuisEisbach, Sybille E.Paulat, MariaGiller, KarinFernandez, Claudio OscarOuteiro, Tiago FBecker, StefanZweckstetter, MarkusamiloideinhibicionsinucleinaPcTShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1α-synuclein is an abundant presynaptic protein that is important for regulation of synaptic vesicle trafficking, and whose misfolding plays a key role in Parkinson’s disease. While α-synuclein is disordered in solution, it folds into a helical conformation when bound to synaptic vesicles. Stabilization of helical, folded α-synuclein might therefore interfere with α-synuclein-induced neurotoxicity. Here we show that several small molecules, which delay aggregation of α-synuclein in solution, including the Parkinson’s disease drug selegiline, fail to interfere with misfolding of vesicle-bound α-synuclein. In contrast, the porphyrin phtalocyanine tetrasulfonate directly binds to vesicle-bound α-synuclein, stabilizes its helical conformation and thereby delays pathogenic misfolding and aggregation. Our study suggests that small-molecule-mediated stabilization of helical vesicle-bound α-synuclein opens new possibilities to target Parkinson’s disease and related synucleinopathiesFil: Fonseca Ornelas, Luis. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Eisbach, Sybille E.. University Medicine; AlemaniaFil: Paulat, Maria. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Giller, Karin. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Fernandez, Claudio Oscar. Universidad Nacional de Rosario; Argentina. Instituto de Investigaciones para el Descubrimiento de Farmacos de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Outeiro, Tiago F. University Medicine; Alemania. University Medical Center; AlemaniaFil: Becker, Stefan. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Zweckstetter, Markus. Max Planck Institute for Biophysical Chemistry; Alemania. University Medical Center; Alemania. German Center for Neurodegenerative Diseases; AlemaniaNature Publishing Group2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29704Fonseca Ornelas, Luis; Eisbach, Sybille E.; Paulat, Maria; Giller, Karin; Fernandez, Claudio Oscar; et al.; Small molecule-mediated stabilization of vesicle-associated helical Alpha-synuclein inhibits patogenic misfolding and aggregation; Nature Publishing Group; Nature Communications; 5; 12-2014; 1-112041-1723CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms6857info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/ncomms6857info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:39Zoai:ri.conicet.gov.ar:11336/29704instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:39.903CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Small molecule-mediated stabilization of vesicle-associated helical Alpha-synuclein inhibits patogenic misfolding and aggregation |
title |
Small molecule-mediated stabilization of vesicle-associated helical Alpha-synuclein inhibits patogenic misfolding and aggregation |
spellingShingle |
Small molecule-mediated stabilization of vesicle-associated helical Alpha-synuclein inhibits patogenic misfolding and aggregation Fonseca Ornelas, Luis amiloide inhibicion sinucleina PcTS |
title_short |
Small molecule-mediated stabilization of vesicle-associated helical Alpha-synuclein inhibits patogenic misfolding and aggregation |
title_full |
Small molecule-mediated stabilization of vesicle-associated helical Alpha-synuclein inhibits patogenic misfolding and aggregation |
title_fullStr |
Small molecule-mediated stabilization of vesicle-associated helical Alpha-synuclein inhibits patogenic misfolding and aggregation |
title_full_unstemmed |
Small molecule-mediated stabilization of vesicle-associated helical Alpha-synuclein inhibits patogenic misfolding and aggregation |
title_sort |
Small molecule-mediated stabilization of vesicle-associated helical Alpha-synuclein inhibits patogenic misfolding and aggregation |
dc.creator.none.fl_str_mv |
Fonseca Ornelas, Luis Eisbach, Sybille E. Paulat, Maria Giller, Karin Fernandez, Claudio Oscar Outeiro, Tiago F Becker, Stefan Zweckstetter, Markus |
author |
Fonseca Ornelas, Luis |
author_facet |
Fonseca Ornelas, Luis Eisbach, Sybille E. Paulat, Maria Giller, Karin Fernandez, Claudio Oscar Outeiro, Tiago F Becker, Stefan Zweckstetter, Markus |
author_role |
author |
author2 |
Eisbach, Sybille E. Paulat, Maria Giller, Karin Fernandez, Claudio Oscar Outeiro, Tiago F Becker, Stefan Zweckstetter, Markus |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
amiloide inhibicion sinucleina PcTS |
topic |
amiloide inhibicion sinucleina PcTS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
α-synuclein is an abundant presynaptic protein that is important for regulation of synaptic vesicle trafficking, and whose misfolding plays a key role in Parkinson’s disease. While α-synuclein is disordered in solution, it folds into a helical conformation when bound to synaptic vesicles. Stabilization of helical, folded α-synuclein might therefore interfere with α-synuclein-induced neurotoxicity. Here we show that several small molecules, which delay aggregation of α-synuclein in solution, including the Parkinson’s disease drug selegiline, fail to interfere with misfolding of vesicle-bound α-synuclein. In contrast, the porphyrin phtalocyanine tetrasulfonate directly binds to vesicle-bound α-synuclein, stabilizes its helical conformation and thereby delays pathogenic misfolding and aggregation. Our study suggests that small-molecule-mediated stabilization of helical vesicle-bound α-synuclein opens new possibilities to target Parkinson’s disease and related synucleinopathies Fil: Fonseca Ornelas, Luis. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Eisbach, Sybille E.. University Medicine; Alemania Fil: Paulat, Maria. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Giller, Karin. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Fernandez, Claudio Oscar. Universidad Nacional de Rosario; Argentina. Instituto de Investigaciones para el Descubrimiento de Farmacos de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Outeiro, Tiago F. University Medicine; Alemania. University Medical Center; Alemania Fil: Becker, Stefan. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Zweckstetter, Markus. Max Planck Institute for Biophysical Chemistry; Alemania. University Medical Center; Alemania. German Center for Neurodegenerative Diseases; Alemania |
description |
α-synuclein is an abundant presynaptic protein that is important for regulation of synaptic vesicle trafficking, and whose misfolding plays a key role in Parkinson’s disease. While α-synuclein is disordered in solution, it folds into a helical conformation when bound to synaptic vesicles. Stabilization of helical, folded α-synuclein might therefore interfere with α-synuclein-induced neurotoxicity. Here we show that several small molecules, which delay aggregation of α-synuclein in solution, including the Parkinson’s disease drug selegiline, fail to interfere with misfolding of vesicle-bound α-synuclein. In contrast, the porphyrin phtalocyanine tetrasulfonate directly binds to vesicle-bound α-synuclein, stabilizes its helical conformation and thereby delays pathogenic misfolding and aggregation. Our study suggests that small-molecule-mediated stabilization of helical vesicle-bound α-synuclein opens new possibilities to target Parkinson’s disease and related synucleinopathies |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/29704 Fonseca Ornelas, Luis; Eisbach, Sybille E.; Paulat, Maria; Giller, Karin; Fernandez, Claudio Oscar; et al.; Small molecule-mediated stabilization of vesicle-associated helical Alpha-synuclein inhibits patogenic misfolding and aggregation; Nature Publishing Group; Nature Communications; 5; 12-2014; 1-11 2041-1723 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/29704 |
identifier_str_mv |
Fonseca Ornelas, Luis; Eisbach, Sybille E.; Paulat, Maria; Giller, Karin; Fernandez, Claudio Oscar; et al.; Small molecule-mediated stabilization of vesicle-associated helical Alpha-synuclein inhibits patogenic misfolding and aggregation; Nature Publishing Group; Nature Communications; 5; 12-2014; 1-11 2041-1723 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms6857 info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/ncomms6857 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842270090347675648 |
score |
13.13397 |