Molecular identification by polymerase chain reaction-restriction fragment length polymorphism of commercially important lithodid species (Crustacea: Anomura) from southern South A...

Autores
Pérez Barros, Patricia; Guzman, Noelia Veronica; Confalonieri, Viviana Andrea; Lovrich, Gustavo Alejandro
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
King crab fisheries constitute a highly profitable marine business in southern South America, where five fisheries for lithodids currently exist, two in Chile and three in Argentina. These fisheries mainly target Lithodes santolla, however in some localities the harvested morphospecies is Lithodes confundens, and in some others it constitutes a mixed fishery since it also captures Paralomis granulosa. Fishery products can be commercialized as frozen shredded or canned minced meat, making specific identification unfeasible since the morphological characters used in the identification are on the carapace, and consequently, not available in these processed products. Therefore, a potential for food fraud exists, since southern king crab meat (L. santolla/ L. confundens) could be replaced by the less expensive false southern king crab, i.e. P. granulosa. Furthermore, incongruence between morphological and genetic characters rendered the taxonomic status of both morphospecies of commercial Lithodes questionable, and stressed the need of implementing molecular methods to monitor the abundance and distribution, as well as the landings, of each genetic clade of L. santolla/ L. confundens. In the present study, we developed rapid and cost-efficient Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) tests that can aid in the identification of commercially important lithodids that inhabit waters off southern South America in cases where identification is not possible using morphological characters.
Fil: Pérez Barros, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina
Fil: Guzman, Noelia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Confalonieri, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Lovrich, Gustavo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Materia
CENTOLLA
CENTOLLÓN
FISHERIES MANAGEMENT
FOOD FRAUD
NAMUNCURÁ MPA/ BURDWOOD BANK
SOUTHERN KING CRAB
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/163519
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular identification by polymerase chain reaction-restriction fragment length polymorphism of commercially important lithodid species (Crustacea: Anomura) from southern South AmericaPérez Barros, PatriciaGuzman, Noelia VeronicaConfalonieri, Viviana AndreaLovrich, Gustavo AlejandroCENTOLLACENTOLLÓNFISHERIES MANAGEMENTFOOD FRAUDNAMUNCURÁ MPA/ BURDWOOD BANKSOUTHERN KING CRABhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1King crab fisheries constitute a highly profitable marine business in southern South America, where five fisheries for lithodids currently exist, two in Chile and three in Argentina. These fisheries mainly target Lithodes santolla, however in some localities the harvested morphospecies is Lithodes confundens, and in some others it constitutes a mixed fishery since it also captures Paralomis granulosa. Fishery products can be commercialized as frozen shredded or canned minced meat, making specific identification unfeasible since the morphological characters used in the identification are on the carapace, and consequently, not available in these processed products. Therefore, a potential for food fraud exists, since southern king crab meat (L. santolla/ L. confundens) could be replaced by the less expensive false southern king crab, i.e. P. granulosa. Furthermore, incongruence between morphological and genetic characters rendered the taxonomic status of both morphospecies of commercial Lithodes questionable, and stressed the need of implementing molecular methods to monitor the abundance and distribution, as well as the landings, of each genetic clade of L. santolla/ L. confundens. In the present study, we developed rapid and cost-efficient Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) tests that can aid in the identification of commercially important lithodids that inhabit waters off southern South America in cases where identification is not possible using morphological characters.Fil: Pérez Barros, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Guzman, Noelia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Confalonieri, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Lovrich, Gustavo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaElsevier Science2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/163519Pérez Barros, Patricia; Guzman, Noelia Veronica; Confalonieri, Viviana Andrea; Lovrich, Gustavo Alejandro; Molecular identification by polymerase chain reaction-restriction fragment length polymorphism of commercially important lithodid species (Crustacea: Anomura) from southern South America; Elsevier Science; Regional Studies in Marine Science; 34; 101027; 2-2020; 1-282352-4855CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2352485519302592info:eu-repo/semantics/altIdentifier/doi/10.1016/j.rsma.2019.101027info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:13Zoai:ri.conicet.gov.ar:11336/163519instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:14.139CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular identification by polymerase chain reaction-restriction fragment length polymorphism of commercially important lithodid species (Crustacea: Anomura) from southern South America
title Molecular identification by polymerase chain reaction-restriction fragment length polymorphism of commercially important lithodid species (Crustacea: Anomura) from southern South America
spellingShingle Molecular identification by polymerase chain reaction-restriction fragment length polymorphism of commercially important lithodid species (Crustacea: Anomura) from southern South America
Pérez Barros, Patricia
CENTOLLA
CENTOLLÓN
FISHERIES MANAGEMENT
FOOD FRAUD
NAMUNCURÁ MPA/ BURDWOOD BANK
SOUTHERN KING CRAB
title_short Molecular identification by polymerase chain reaction-restriction fragment length polymorphism of commercially important lithodid species (Crustacea: Anomura) from southern South America
title_full Molecular identification by polymerase chain reaction-restriction fragment length polymorphism of commercially important lithodid species (Crustacea: Anomura) from southern South America
title_fullStr Molecular identification by polymerase chain reaction-restriction fragment length polymorphism of commercially important lithodid species (Crustacea: Anomura) from southern South America
title_full_unstemmed Molecular identification by polymerase chain reaction-restriction fragment length polymorphism of commercially important lithodid species (Crustacea: Anomura) from southern South America
title_sort Molecular identification by polymerase chain reaction-restriction fragment length polymorphism of commercially important lithodid species (Crustacea: Anomura) from southern South America
dc.creator.none.fl_str_mv Pérez Barros, Patricia
Guzman, Noelia Veronica
Confalonieri, Viviana Andrea
Lovrich, Gustavo Alejandro
author Pérez Barros, Patricia
author_facet Pérez Barros, Patricia
Guzman, Noelia Veronica
Confalonieri, Viviana Andrea
Lovrich, Gustavo Alejandro
author_role author
author2 Guzman, Noelia Veronica
Confalonieri, Viviana Andrea
Lovrich, Gustavo Alejandro
author2_role author
author
author
dc.subject.none.fl_str_mv CENTOLLA
CENTOLLÓN
FISHERIES MANAGEMENT
FOOD FRAUD
NAMUNCURÁ MPA/ BURDWOOD BANK
SOUTHERN KING CRAB
topic CENTOLLA
CENTOLLÓN
FISHERIES MANAGEMENT
FOOD FRAUD
NAMUNCURÁ MPA/ BURDWOOD BANK
SOUTHERN KING CRAB
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv King crab fisheries constitute a highly profitable marine business in southern South America, where five fisheries for lithodids currently exist, two in Chile and three in Argentina. These fisheries mainly target Lithodes santolla, however in some localities the harvested morphospecies is Lithodes confundens, and in some others it constitutes a mixed fishery since it also captures Paralomis granulosa. Fishery products can be commercialized as frozen shredded or canned minced meat, making specific identification unfeasible since the morphological characters used in the identification are on the carapace, and consequently, not available in these processed products. Therefore, a potential for food fraud exists, since southern king crab meat (L. santolla/ L. confundens) could be replaced by the less expensive false southern king crab, i.e. P. granulosa. Furthermore, incongruence between morphological and genetic characters rendered the taxonomic status of both morphospecies of commercial Lithodes questionable, and stressed the need of implementing molecular methods to monitor the abundance and distribution, as well as the landings, of each genetic clade of L. santolla/ L. confundens. In the present study, we developed rapid and cost-efficient Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) tests that can aid in the identification of commercially important lithodids that inhabit waters off southern South America in cases where identification is not possible using morphological characters.
Fil: Pérez Barros, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina
Fil: Guzman, Noelia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Confalonieri, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Lovrich, Gustavo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
description King crab fisheries constitute a highly profitable marine business in southern South America, where five fisheries for lithodids currently exist, two in Chile and three in Argentina. These fisheries mainly target Lithodes santolla, however in some localities the harvested morphospecies is Lithodes confundens, and in some others it constitutes a mixed fishery since it also captures Paralomis granulosa. Fishery products can be commercialized as frozen shredded or canned minced meat, making specific identification unfeasible since the morphological characters used in the identification are on the carapace, and consequently, not available in these processed products. Therefore, a potential for food fraud exists, since southern king crab meat (L. santolla/ L. confundens) could be replaced by the less expensive false southern king crab, i.e. P. granulosa. Furthermore, incongruence between morphological and genetic characters rendered the taxonomic status of both morphospecies of commercial Lithodes questionable, and stressed the need of implementing molecular methods to monitor the abundance and distribution, as well as the landings, of each genetic clade of L. santolla/ L. confundens. In the present study, we developed rapid and cost-efficient Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) tests that can aid in the identification of commercially important lithodids that inhabit waters off southern South America in cases where identification is not possible using morphological characters.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/163519
Pérez Barros, Patricia; Guzman, Noelia Veronica; Confalonieri, Viviana Andrea; Lovrich, Gustavo Alejandro; Molecular identification by polymerase chain reaction-restriction fragment length polymorphism of commercially important lithodid species (Crustacea: Anomura) from southern South America; Elsevier Science; Regional Studies in Marine Science; 34; 101027; 2-2020; 1-28
2352-4855
CONICET Digital
CONICET
url http://hdl.handle.net/11336/163519
identifier_str_mv Pérez Barros, Patricia; Guzman, Noelia Veronica; Confalonieri, Viviana Andrea; Lovrich, Gustavo Alejandro; Molecular identification by polymerase chain reaction-restriction fragment length polymorphism of commercially important lithodid species (Crustacea: Anomura) from southern South America; Elsevier Science; Regional Studies in Marine Science; 34; 101027; 2-2020; 1-28
2352-4855
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2352485519302592
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.rsma.2019.101027
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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