Nitric oxide is essential for vesicle formation and trafficking in Arabidopsis root hair growth

Autores
Lombardo, Maria Cristina; Lamattina, Lorenzo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The functions of Nitric Oxide (NO) in processes associated to root hair growth in Arabidopsis were analyzed. NO is located at high concentrations in the root hair cell files at any stage of development. NO is detected inside of the vacuole in immature actively growing root hairs and later, NO is localized in cytoplasm when they become mature. Experiments performed by depleting NO in Arabidopsis root hairs indicate that NO is required for endocytosis process, vesicle formation and trafficking, and it is not involved in nucleus migration, vacuolar development and transvacuolar strands. The Arabidopsis G?4,3 mutant (double mutant nia1/nia2) is severely impaired in NO production and generates smaller root hairs than wt. Root hairs from Arabidopsis G?4,3 mutant show altered vesicular trafficking and are reminiscent of NO-depleted root hairs from Arabidopsis wt. Interestingly, normal vesicle formation and trafficking as well as root hair growth is restored by exogenous NO application in Arabidopsis G?4,3 mutant. All together, these results firmly support the essential role played by NO in Arabidopsis root hair growing process.
Fil: Lombardo, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
ACTIN
ARABIDOPSIS G'4,3 MUTANT
ENDOCYTOSIS
NITRIC OXIDE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103582
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Nitric oxide is essential for vesicle formation and trafficking in Arabidopsis root hair growthLombardo, Maria CristinaLamattina, LorenzoACTINARABIDOPSIS G'4,3 MUTANTENDOCYTOSISNITRIC OXIDEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The functions of Nitric Oxide (NO) in processes associated to root hair growth in Arabidopsis were analyzed. NO is located at high concentrations in the root hair cell files at any stage of development. NO is detected inside of the vacuole in immature actively growing root hairs and later, NO is localized in cytoplasm when they become mature. Experiments performed by depleting NO in Arabidopsis root hairs indicate that NO is required for endocytosis process, vesicle formation and trafficking, and it is not involved in nucleus migration, vacuolar development and transvacuolar strands. The Arabidopsis G?4,3 mutant (double mutant nia1/nia2) is severely impaired in NO production and generates smaller root hairs than wt. Root hairs from Arabidopsis G?4,3 mutant show altered vesicular trafficking and are reminiscent of NO-depleted root hairs from Arabidopsis wt. Interestingly, normal vesicle formation and trafficking as well as root hair growth is restored by exogenous NO application in Arabidopsis G?4,3 mutant. All together, these results firmly support the essential role played by NO in Arabidopsis root hair growing process.Fil: Lombardo, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaOxford University Press2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103582Lombardo, Maria Cristina; Lamattina, Lorenzo; Nitric oxide is essential for vesicle formation and trafficking in Arabidopsis root hair growth; Oxford University Press; Journal of Experimental Botany; 63; 13; 3-2012; 4875-48850022-0957CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/ers166info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article/63/13/4875/486922info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:34:07Zoai:ri.conicet.gov.ar:11336/103582instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:34:08.146CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nitric oxide is essential for vesicle formation and trafficking in Arabidopsis root hair growth
title Nitric oxide is essential for vesicle formation and trafficking in Arabidopsis root hair growth
spellingShingle Nitric oxide is essential for vesicle formation and trafficking in Arabidopsis root hair growth
Lombardo, Maria Cristina
ACTIN
ARABIDOPSIS G'4,3 MUTANT
ENDOCYTOSIS
NITRIC OXIDE
title_short Nitric oxide is essential for vesicle formation and trafficking in Arabidopsis root hair growth
title_full Nitric oxide is essential for vesicle formation and trafficking in Arabidopsis root hair growth
title_fullStr Nitric oxide is essential for vesicle formation and trafficking in Arabidopsis root hair growth
title_full_unstemmed Nitric oxide is essential for vesicle formation and trafficking in Arabidopsis root hair growth
title_sort Nitric oxide is essential for vesicle formation and trafficking in Arabidopsis root hair growth
dc.creator.none.fl_str_mv Lombardo, Maria Cristina
Lamattina, Lorenzo
author Lombardo, Maria Cristina
author_facet Lombardo, Maria Cristina
Lamattina, Lorenzo
author_role author
author2 Lamattina, Lorenzo
author2_role author
dc.subject.none.fl_str_mv ACTIN
ARABIDOPSIS G'4,3 MUTANT
ENDOCYTOSIS
NITRIC OXIDE
topic ACTIN
ARABIDOPSIS G'4,3 MUTANT
ENDOCYTOSIS
NITRIC OXIDE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The functions of Nitric Oxide (NO) in processes associated to root hair growth in Arabidopsis were analyzed. NO is located at high concentrations in the root hair cell files at any stage of development. NO is detected inside of the vacuole in immature actively growing root hairs and later, NO is localized in cytoplasm when they become mature. Experiments performed by depleting NO in Arabidopsis root hairs indicate that NO is required for endocytosis process, vesicle formation and trafficking, and it is not involved in nucleus migration, vacuolar development and transvacuolar strands. The Arabidopsis G?4,3 mutant (double mutant nia1/nia2) is severely impaired in NO production and generates smaller root hairs than wt. Root hairs from Arabidopsis G?4,3 mutant show altered vesicular trafficking and are reminiscent of NO-depleted root hairs from Arabidopsis wt. Interestingly, normal vesicle formation and trafficking as well as root hair growth is restored by exogenous NO application in Arabidopsis G?4,3 mutant. All together, these results firmly support the essential role played by NO in Arabidopsis root hair growing process.
Fil: Lombardo, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description The functions of Nitric Oxide (NO) in processes associated to root hair growth in Arabidopsis were analyzed. NO is located at high concentrations in the root hair cell files at any stage of development. NO is detected inside of the vacuole in immature actively growing root hairs and later, NO is localized in cytoplasm when they become mature. Experiments performed by depleting NO in Arabidopsis root hairs indicate that NO is required for endocytosis process, vesicle formation and trafficking, and it is not involved in nucleus migration, vacuolar development and transvacuolar strands. The Arabidopsis G?4,3 mutant (double mutant nia1/nia2) is severely impaired in NO production and generates smaller root hairs than wt. Root hairs from Arabidopsis G?4,3 mutant show altered vesicular trafficking and are reminiscent of NO-depleted root hairs from Arabidopsis wt. Interestingly, normal vesicle formation and trafficking as well as root hair growth is restored by exogenous NO application in Arabidopsis G?4,3 mutant. All together, these results firmly support the essential role played by NO in Arabidopsis root hair growing process.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103582
Lombardo, Maria Cristina; Lamattina, Lorenzo; Nitric oxide is essential for vesicle formation and trafficking in Arabidopsis root hair growth; Oxford University Press; Journal of Experimental Botany; 63; 13; 3-2012; 4875-4885
0022-0957
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103582
identifier_str_mv Lombardo, Maria Cristina; Lamattina, Lorenzo; Nitric oxide is essential for vesicle formation and trafficking in Arabidopsis root hair growth; Oxford University Press; Journal of Experimental Botany; 63; 13; 3-2012; 4875-4885
0022-0957
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/ers166
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article/63/13/4875/486922
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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