Transcriptional regulation of the salmonella enterica std fimbriaoperon by the RcsCDB system

Autores
Farizano, Juan Vicente; García-Pastor, Lucia; Casadesús, Josep; Delgado, Monica Alejandra
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Salmonella enterica serovar Typhimurium, the RcsCDB regulatory system controls the expression of genes involved in synthesis of colanic acid, formation of flagella and virulence. Here, we show that activation of the RcsCDB system downregulates expression of std, an operon that encodes fimbriae involved in Salmonella attachment to the mucus layer in the large intestine. Bioinformatic analysis predicts the existence of an RcsB-binding site located 180 bp upstream to the +1 transcription start site of the std promoter, and electrophoretic mobility shift assays confirm that RcsB binds the std promoter region in vitro. This study adds RcsB to the list of regulators of std transcription and provides an example of modulation of fimbriae synthesis by a signal transduction system.
Fil: Farizano, Juan Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: García-Pastor, Lucia. Universidad de Sevilla; España
Fil: Casadesús, Josep. Universidad de Sevilla; España
Fil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
RCSCDB
SALMONELLA
STD FIMBRIAE
TRANSCRIPTIONAL CONTROL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/219139
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/219139
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Transcriptional regulation of the salmonella enterica std fimbriaoperon by the RcsCDB systemFarizano, Juan VicenteGarcía-Pastor, LuciaCasadesús, JosepDelgado, Monica AlejandraRCSCDBSALMONELLASTD FIMBRIAETRANSCRIPTIONAL CONTROLhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In Salmonella enterica serovar Typhimurium, the RcsCDB regulatory system controls the expression of genes involved in synthesis of colanic acid, formation of flagella and virulence. Here, we show that activation of the RcsCDB system downregulates expression of std, an operon that encodes fimbriae involved in Salmonella attachment to the mucus layer in the large intestine. Bioinformatic analysis predicts the existence of an RcsB-binding site located 180 bp upstream to the +1 transcription start site of the std promoter, and electrophoretic mobility shift assays confirm that RcsB binds the std promoter region in vitro. This study adds RcsB to the list of regulators of std transcription and provides an example of modulation of fimbriae synthesis by a signal transduction system.Fil: Farizano, Juan Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: García-Pastor, Lucia. Universidad de Sevilla; EspañaFil: Casadesús, Josep. Universidad de Sevilla; EspañaFil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaSociety for General Microbiology2019-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/219139Farizano, Juan Vicente; García-Pastor, Lucia; Casadesús, Josep; Delgado, Monica Alejandra; Transcriptional regulation of the salmonella enterica std fimbriaoperon by the RcsCDB system; Society for General Microbiology; Microbiology-UK; 165; 11; 11-2019; 1245-12501350-0872CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000854info:eu-repo/semantics/altIdentifier/doi/10.1099/mic.0.000854info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:21:10Zoai:ri.conicet.gov.ar:11336/219139instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:21:10.809CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transcriptional regulation of the salmonella enterica std fimbriaoperon by the RcsCDB system
title Transcriptional regulation of the salmonella enterica std fimbriaoperon by the RcsCDB system
spellingShingle Transcriptional regulation of the salmonella enterica std fimbriaoperon by the RcsCDB system
Farizano, Juan Vicente
RCSCDB
SALMONELLA
STD FIMBRIAE
TRANSCRIPTIONAL CONTROL
title_short Transcriptional regulation of the salmonella enterica std fimbriaoperon by the RcsCDB system
title_full Transcriptional regulation of the salmonella enterica std fimbriaoperon by the RcsCDB system
title_fullStr Transcriptional regulation of the salmonella enterica std fimbriaoperon by the RcsCDB system
title_full_unstemmed Transcriptional regulation of the salmonella enterica std fimbriaoperon by the RcsCDB system
title_sort Transcriptional regulation of the salmonella enterica std fimbriaoperon by the RcsCDB system
dc.creator.none.fl_str_mv Farizano, Juan Vicente
García-Pastor, Lucia
Casadesús, Josep
Delgado, Monica Alejandra
author Farizano, Juan Vicente
author_facet Farizano, Juan Vicente
García-Pastor, Lucia
Casadesús, Josep
Delgado, Monica Alejandra
author_role author
author2 García-Pastor, Lucia
Casadesús, Josep
Delgado, Monica Alejandra
author2_role author
author
author
dc.subject.none.fl_str_mv RCSCDB
SALMONELLA
STD FIMBRIAE
TRANSCRIPTIONAL CONTROL
topic RCSCDB
SALMONELLA
STD FIMBRIAE
TRANSCRIPTIONAL CONTROL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In Salmonella enterica serovar Typhimurium, the RcsCDB regulatory system controls the expression of genes involved in synthesis of colanic acid, formation of flagella and virulence. Here, we show that activation of the RcsCDB system downregulates expression of std, an operon that encodes fimbriae involved in Salmonella attachment to the mucus layer in the large intestine. Bioinformatic analysis predicts the existence of an RcsB-binding site located 180 bp upstream to the +1 transcription start site of the std promoter, and electrophoretic mobility shift assays confirm that RcsB binds the std promoter region in vitro. This study adds RcsB to the list of regulators of std transcription and provides an example of modulation of fimbriae synthesis by a signal transduction system.
Fil: Farizano, Juan Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: García-Pastor, Lucia. Universidad de Sevilla; España
Fil: Casadesús, Josep. Universidad de Sevilla; España
Fil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description In Salmonella enterica serovar Typhimurium, the RcsCDB regulatory system controls the expression of genes involved in synthesis of colanic acid, formation of flagella and virulence. Here, we show that activation of the RcsCDB system downregulates expression of std, an operon that encodes fimbriae involved in Salmonella attachment to the mucus layer in the large intestine. Bioinformatic analysis predicts the existence of an RcsB-binding site located 180 bp upstream to the +1 transcription start site of the std promoter, and electrophoretic mobility shift assays confirm that RcsB binds the std promoter region in vitro. This study adds RcsB to the list of regulators of std transcription and provides an example of modulation of fimbriae synthesis by a signal transduction system.
publishDate 2019
dc.date.none.fl_str_mv 2019-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/219139
Farizano, Juan Vicente; García-Pastor, Lucia; Casadesús, Josep; Delgado, Monica Alejandra; Transcriptional regulation of the salmonella enterica std fimbriaoperon by the RcsCDB system; Society for General Microbiology; Microbiology-UK; 165; 11; 11-2019; 1245-1250
1350-0872
CONICET Digital
CONICET
url http://hdl.handle.net/11336/219139
identifier_str_mv Farizano, Juan Vicente; García-Pastor, Lucia; Casadesús, Josep; Delgado, Monica Alejandra; Transcriptional regulation of the salmonella enterica std fimbriaoperon by the RcsCDB system; Society for General Microbiology; Microbiology-UK; 165; 11; 11-2019; 1245-1250
1350-0872
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000854
info:eu-repo/semantics/altIdentifier/doi/10.1099/mic.0.000854
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for General Microbiology
publisher.none.fl_str_mv Society for General Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082597859360768
score 13.22299

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