Molecular discrimination of bacteria (organic versus mineral soil layers) of dry woodlands of Argentina

Autores
Noe, Laura Belén; Ascher, J.; Ceccherini, M. T.; Abril, Adriana; Pietramellara, G.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We performed comparative molecular screening of above- and belowground soil bacteria and focused on key functional community members involved in organic matter turn-over, to assess changes due to different management and climatic conditions. Aboveground organic residues (comprising plant- and animal residues) and bulk soil were sampled in summer and winter from two land use strategies (grazed vs un-grazed) and two dry forests of different climate zones of Argentina (Arid Chaco vs Monte). Genetic fingerprinting using polymerase chain reaction - denaturing gradient gel electrophoresis (PCR-DGGE) of bacterial communities, actinomycetes, ammonium oxidizers and N2 fixers was performed and interpreted as function of multiple environmental factors. The similarity differences in bacterial community structures in all analyzed conditions ranged from 12 to 85% and were consistent with i) climatic conditions in the two sampling sites; ii) the impact of land use in arid areas; iii) the temporal heterogeneity of the drylands; and iv) chemical differences between soil and aboveground organic residues. Our results revealed distinct structures of above- and belowground soil bacteria and specific multiple factor responses to environmental changes (natural vs anthropogenic),supporting the capability of comparative molecular screening to gain insights into above- and belowground soil organic matter dynamics.
Fil: Noe, Laura Belén. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Departamento de Recursos Naturales; Argentina
Fil: Ascher, J.. Università degli Studi di Firenze; Italia
Fil: Ceccherini, M. T.. Università degli Studi di Firenze; Italia
Fil: Abril, Adriana. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Departamento de Recursos Naturales; Argentina
Fil: Pietramellara, G.. Università degli Studi di Firenze; Italia
Materia
Acinomycetes
Ammonium oxidizers
Arid soil
Soil Organic Matter
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272557
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Molecular discrimination of bacteria (organic versus mineral soil layers) of dry woodlands of ArgentinaNoe, Laura BelénAscher, J.Ceccherini, M. T.Abril, AdrianaPietramellara, G.AcinomycetesAmmonium oxidizersArid soilSoil Organic Matterhttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4We performed comparative molecular screening of above- and belowground soil bacteria and focused on key functional community members involved in organic matter turn-over, to assess changes due to different management and climatic conditions. Aboveground organic residues (comprising plant- and animal residues) and bulk soil were sampled in summer and winter from two land use strategies (grazed vs un-grazed) and two dry forests of different climate zones of Argentina (Arid Chaco vs Monte). Genetic fingerprinting using polymerase chain reaction - denaturing gradient gel electrophoresis (PCR-DGGE) of bacterial communities, actinomycetes, ammonium oxidizers and N2 fixers was performed and interpreted as function of multiple environmental factors. The similarity differences in bacterial community structures in all analyzed conditions ranged from 12 to 85% and were consistent with i) climatic conditions in the two sampling sites; ii) the impact of land use in arid areas; iii) the temporal heterogeneity of the drylands; and iv) chemical differences between soil and aboveground organic residues. Our results revealed distinct structures of above- and belowground soil bacteria and specific multiple factor responses to environmental changes (natural vs anthropogenic),supporting the capability of comparative molecular screening to gain insights into above- and belowground soil organic matter dynamics.Fil: Noe, Laura Belén. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Departamento de Recursos Naturales; ArgentinaFil: Ascher, J.. Università degli Studi di Firenze; ItaliaFil: Ceccherini, M. T.. Università degli Studi di Firenze; ItaliaFil: Abril, Adriana. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Departamento de Recursos Naturales; ArgentinaFil: Pietramellara, G.. Università degli Studi di Firenze; ItaliaAcademic Press Ltd - Elsevier Science Ltd2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272557Noe, Laura Belén; Ascher, J.; Ceccherini, M. T.; Abril, Adriana; Pietramellara, G.; Molecular discrimination of bacteria (organic versus mineral soil layers) of dry woodlands of Argentina; Academic Press Ltd - Elsevier Science Ltd; Journal of Arid Environments; 85; 10-2012; 18-260140-1963CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0140196312001358info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jaridenv.2012.04.007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T12:27:11Zoai:ri.conicet.gov.ar:11336/272557instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 12:27:11.34CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular discrimination of bacteria (organic versus mineral soil layers) of dry woodlands of Argentina
title Molecular discrimination of bacteria (organic versus mineral soil layers) of dry woodlands of Argentina
spellingShingle Molecular discrimination of bacteria (organic versus mineral soil layers) of dry woodlands of Argentina
Noe, Laura Belén
Acinomycetes
Ammonium oxidizers
Arid soil
Soil Organic Matter
title_short Molecular discrimination of bacteria (organic versus mineral soil layers) of dry woodlands of Argentina
title_full Molecular discrimination of bacteria (organic versus mineral soil layers) of dry woodlands of Argentina
title_fullStr Molecular discrimination of bacteria (organic versus mineral soil layers) of dry woodlands of Argentina
title_full_unstemmed Molecular discrimination of bacteria (organic versus mineral soil layers) of dry woodlands of Argentina
title_sort Molecular discrimination of bacteria (organic versus mineral soil layers) of dry woodlands of Argentina
dc.creator.none.fl_str_mv Noe, Laura Belén
Ascher, J.
Ceccherini, M. T.
Abril, Adriana
Pietramellara, G.
author Noe, Laura Belén
author_facet Noe, Laura Belén
Ascher, J.
Ceccherini, M. T.
Abril, Adriana
Pietramellara, G.
author_role author
author2 Ascher, J.
Ceccherini, M. T.
Abril, Adriana
Pietramellara, G.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Acinomycetes
Ammonium oxidizers
Arid soil
Soil Organic Matter
topic Acinomycetes
Ammonium oxidizers
Arid soil
Soil Organic Matter
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv We performed comparative molecular screening of above- and belowground soil bacteria and focused on key functional community members involved in organic matter turn-over, to assess changes due to different management and climatic conditions. Aboveground organic residues (comprising plant- and animal residues) and bulk soil were sampled in summer and winter from two land use strategies (grazed vs un-grazed) and two dry forests of different climate zones of Argentina (Arid Chaco vs Monte). Genetic fingerprinting using polymerase chain reaction - denaturing gradient gel electrophoresis (PCR-DGGE) of bacterial communities, actinomycetes, ammonium oxidizers and N2 fixers was performed and interpreted as function of multiple environmental factors. The similarity differences in bacterial community structures in all analyzed conditions ranged from 12 to 85% and were consistent with i) climatic conditions in the two sampling sites; ii) the impact of land use in arid areas; iii) the temporal heterogeneity of the drylands; and iv) chemical differences between soil and aboveground organic residues. Our results revealed distinct structures of above- and belowground soil bacteria and specific multiple factor responses to environmental changes (natural vs anthropogenic),supporting the capability of comparative molecular screening to gain insights into above- and belowground soil organic matter dynamics.
Fil: Noe, Laura Belén. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Departamento de Recursos Naturales; Argentina
Fil: Ascher, J.. Università degli Studi di Firenze; Italia
Fil: Ceccherini, M. T.. Università degli Studi di Firenze; Italia
Fil: Abril, Adriana. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Departamento de Recursos Naturales; Argentina
Fil: Pietramellara, G.. Università degli Studi di Firenze; Italia
description We performed comparative molecular screening of above- and belowground soil bacteria and focused on key functional community members involved in organic matter turn-over, to assess changes due to different management and climatic conditions. Aboveground organic residues (comprising plant- and animal residues) and bulk soil were sampled in summer and winter from two land use strategies (grazed vs un-grazed) and two dry forests of different climate zones of Argentina (Arid Chaco vs Monte). Genetic fingerprinting using polymerase chain reaction - denaturing gradient gel electrophoresis (PCR-DGGE) of bacterial communities, actinomycetes, ammonium oxidizers and N2 fixers was performed and interpreted as function of multiple environmental factors. The similarity differences in bacterial community structures in all analyzed conditions ranged from 12 to 85% and were consistent with i) climatic conditions in the two sampling sites; ii) the impact of land use in arid areas; iii) the temporal heterogeneity of the drylands; and iv) chemical differences between soil and aboveground organic residues. Our results revealed distinct structures of above- and belowground soil bacteria and specific multiple factor responses to environmental changes (natural vs anthropogenic),supporting the capability of comparative molecular screening to gain insights into above- and belowground soil organic matter dynamics.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272557
Noe, Laura Belén; Ascher, J.; Ceccherini, M. T.; Abril, Adriana; Pietramellara, G.; Molecular discrimination of bacteria (organic versus mineral soil layers) of dry woodlands of Argentina; Academic Press Ltd - Elsevier Science Ltd; Journal of Arid Environments; 85; 10-2012; 18-26
0140-1963
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272557
identifier_str_mv Noe, Laura Belén; Ascher, J.; Ceccherini, M. T.; Abril, Adriana; Pietramellara, G.; Molecular discrimination of bacteria (organic versus mineral soil layers) of dry woodlands of Argentina; Academic Press Ltd - Elsevier Science Ltd; Journal of Arid Environments; 85; 10-2012; 18-26
0140-1963
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0140196312001358
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jaridenv.2012.04.007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.589754

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