Increasing nitrate/ammonium ratio for improvement of garlic micropropagation

Autores
Luciani, Gabriela Fabiana; Marinangeli, Pablo Alejandro; Curvetto, Nestor Raul
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of different culture media with different growth regulator and nitrate/ammonium ratios on split garlic shoots was studied to obtain an optimized micropropagation protocol. Three basal media: MS, BDS and BLM (i.e. BDS modified to obtain a nitrate:ammonium ratio of 35 mM:8 mM) combined with two concentrations of NAA (0.5 or 5 μM) and BAP (1 or 10 μM) were evaluated in three Colorado garlic clones: Español Selección Ascasubi (ESA), Español Selección Médanos (ESM) and I 50. In BLM medium with 5 μM NAA and 10 μM BAP the highest multiplication rates were 140, 542 and 743 shoots per split shoot after three subcultures in 140 days for clones ESA, ESM and I 50, respectively. Comparing the mean rates obtained in MS medium with those obtained in BDS medium in the same time, the latter produced 1.5-, 4- and 2.2-fold increases in the mean multiplication rates for ESA, ESM and I 50 clones, respectively; while BLM produced 5-, 3- and 10-fold increases in the mean multiplication rates. Since BDS and BLM media only differ in their NO3−:NH4+ ratio we can conclude that the differences in the multiplication rates could probably be due to an increase in the NO3− level of the BLM medium and so the multiplication ratios for these garlic clones can be greatly improved by the use of higher levels of nitrogen supplied as nitrate (35mMNO3−:8mMNH4+ ratio) with higher concentrations of growth regulators such as 5 μM NAA and 10 μM BAP.
Fil: Luciani, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Marinangeli, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Materia
Allium Sativum
Splitting
Growth Regulators
Nitrate:Ammonium Ratio
Micropropagation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30141
Acceder
id CONICETDig_4f964dcf144bb8f3a12dee3c9c24aada
oai_identifier_str oai:ri.conicet.gov.ar:11336/30141
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Increasing nitrate/ammonium ratio for improvement of garlic micropropagationLuciani, Gabriela FabianaMarinangeli, Pablo AlejandroCurvetto, Nestor RaulAllium SativumSplittingGrowth RegulatorsNitrate:Ammonium RatioMicropropagationhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The effect of different culture media with different growth regulator and nitrate/ammonium ratios on split garlic shoots was studied to obtain an optimized micropropagation protocol. Three basal media: MS, BDS and BLM (i.e. BDS modified to obtain a nitrate:ammonium ratio of 35 mM:8 mM) combined with two concentrations of NAA (0.5 or 5 μM) and BAP (1 or 10 μM) were evaluated in three Colorado garlic clones: Español Selección Ascasubi (ESA), Español Selección Médanos (ESM) and I 50. In BLM medium with 5 μM NAA and 10 μM BAP the highest multiplication rates were 140, 542 and 743 shoots per split shoot after three subcultures in 140 days for clones ESA, ESM and I 50, respectively. Comparing the mean rates obtained in MS medium with those obtained in BDS medium in the same time, the latter produced 1.5-, 4- and 2.2-fold increases in the mean multiplication rates for ESA, ESM and I 50 clones, respectively; while BLM produced 5-, 3- and 10-fold increases in the mean multiplication rates. Since BDS and BLM media only differ in their NO3−:NH4+ ratio we can conclude that the differences in the multiplication rates could probably be due to an increase in the NO3− level of the BLM medium and so the multiplication ratios for these garlic clones can be greatly improved by the use of higher levels of nitrogen supplied as nitrate (35mMNO3−:8mMNH4+ ratio) with higher concentrations of growth regulators such as 5 μM NAA and 10 μM BAP.Fil: Luciani, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Marinangeli, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaElsevier Science2001-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30141Luciani, Gabriela Fabiana; Marinangeli, Pablo Alejandro; Curvetto, Nestor Raul; Increasing nitrate/ammonium ratio for improvement of garlic micropropagation; Elsevier Science; Scientia Horticulturae; 87; 1-2; 1-2001; 11-200304-4238CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304423800001655info:eu-repo/semantics/altIdentifier/doi/10.1016/S0304-4238(00)00165-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:03:19Zoai:ri.conicet.gov.ar:11336/30141instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:03:19.785CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Increasing nitrate/ammonium ratio for improvement of garlic micropropagation
title Increasing nitrate/ammonium ratio for improvement of garlic micropropagation
spellingShingle Increasing nitrate/ammonium ratio for improvement of garlic micropropagation
Luciani, Gabriela Fabiana
Allium Sativum
Splitting
Growth Regulators
Nitrate:Ammonium Ratio
Micropropagation
title_short Increasing nitrate/ammonium ratio for improvement of garlic micropropagation
title_full Increasing nitrate/ammonium ratio for improvement of garlic micropropagation
title_fullStr Increasing nitrate/ammonium ratio for improvement of garlic micropropagation
title_full_unstemmed Increasing nitrate/ammonium ratio for improvement of garlic micropropagation
title_sort Increasing nitrate/ammonium ratio for improvement of garlic micropropagation
dc.creator.none.fl_str_mv Luciani, Gabriela Fabiana
Marinangeli, Pablo Alejandro
Curvetto, Nestor Raul
author Luciani, Gabriela Fabiana
author_facet Luciani, Gabriela Fabiana
Marinangeli, Pablo Alejandro
Curvetto, Nestor Raul
author_role author
author2 Marinangeli, Pablo Alejandro
Curvetto, Nestor Raul
author2_role author
author
dc.subject.none.fl_str_mv Allium Sativum
Splitting
Growth Regulators
Nitrate:Ammonium Ratio
Micropropagation
topic Allium Sativum
Splitting
Growth Regulators
Nitrate:Ammonium Ratio
Micropropagation
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The effect of different culture media with different growth regulator and nitrate/ammonium ratios on split garlic shoots was studied to obtain an optimized micropropagation protocol. Three basal media: MS, BDS and BLM (i.e. BDS modified to obtain a nitrate:ammonium ratio of 35 mM:8 mM) combined with two concentrations of NAA (0.5 or 5 μM) and BAP (1 or 10 μM) were evaluated in three Colorado garlic clones: Español Selección Ascasubi (ESA), Español Selección Médanos (ESM) and I 50. In BLM medium with 5 μM NAA and 10 μM BAP the highest multiplication rates were 140, 542 and 743 shoots per split shoot after three subcultures in 140 days for clones ESA, ESM and I 50, respectively. Comparing the mean rates obtained in MS medium with those obtained in BDS medium in the same time, the latter produced 1.5-, 4- and 2.2-fold increases in the mean multiplication rates for ESA, ESM and I 50 clones, respectively; while BLM produced 5-, 3- and 10-fold increases in the mean multiplication rates. Since BDS and BLM media only differ in their NO3−:NH4+ ratio we can conclude that the differences in the multiplication rates could probably be due to an increase in the NO3− level of the BLM medium and so the multiplication ratios for these garlic clones can be greatly improved by the use of higher levels of nitrogen supplied as nitrate (35mMNO3−:8mMNH4+ ratio) with higher concentrations of growth regulators such as 5 μM NAA and 10 μM BAP.
Fil: Luciani, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Marinangeli, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
description The effect of different culture media with different growth regulator and nitrate/ammonium ratios on split garlic shoots was studied to obtain an optimized micropropagation protocol. Three basal media: MS, BDS and BLM (i.e. BDS modified to obtain a nitrate:ammonium ratio of 35 mM:8 mM) combined with two concentrations of NAA (0.5 or 5 μM) and BAP (1 or 10 μM) were evaluated in three Colorado garlic clones: Español Selección Ascasubi (ESA), Español Selección Médanos (ESM) and I 50. In BLM medium with 5 μM NAA and 10 μM BAP the highest multiplication rates were 140, 542 and 743 shoots per split shoot after three subcultures in 140 days for clones ESA, ESM and I 50, respectively. Comparing the mean rates obtained in MS medium with those obtained in BDS medium in the same time, the latter produced 1.5-, 4- and 2.2-fold increases in the mean multiplication rates for ESA, ESM and I 50 clones, respectively; while BLM produced 5-, 3- and 10-fold increases in the mean multiplication rates. Since BDS and BLM media only differ in their NO3−:NH4+ ratio we can conclude that the differences in the multiplication rates could probably be due to an increase in the NO3− level of the BLM medium and so the multiplication ratios for these garlic clones can be greatly improved by the use of higher levels of nitrogen supplied as nitrate (35mMNO3−:8mMNH4+ ratio) with higher concentrations of growth regulators such as 5 μM NAA and 10 μM BAP.
publishDate 2001
dc.date.none.fl_str_mv 2001-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30141
Luciani, Gabriela Fabiana; Marinangeli, Pablo Alejandro; Curvetto, Nestor Raul; Increasing nitrate/ammonium ratio for improvement of garlic micropropagation; Elsevier Science; Scientia Horticulturae; 87; 1-2; 1-2001; 11-20
0304-4238
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30141
identifier_str_mv Luciani, Gabriela Fabiana; Marinangeli, Pablo Alejandro; Curvetto, Nestor Raul; Increasing nitrate/ammonium ratio for improvement of garlic micropropagation; Elsevier Science; Scientia Horticulturae; 87; 1-2; 1-2001; 11-20
0304-4238
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304423800001655
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0304-4238(00)00165-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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