The Conditions of Ovary Storage Affect the Quality of Porcine Oocytes

Autores
Tellado, Matías Nicolás; Alvarez, Gabriel Martín; Dalvit, Gabriel Carlos; Cetica, Pablo Daniel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We studied the effect of different storage conditions of porcine ovaries (time, temperature) on the characteristics of the follicular fluid, immature oocyte quality, meiotic competence and in vitro fertilization of oocytes. Ovaries were stored for 2, 4 or 6 h at 3 different temperatures (15˚C, 25˚C or 35˚C). As the storage time increased, pH and glucose concentration of the follicular fluid, percentage of live immature oocytes at germinal vesicle stage, oxidative activity and maturation rate decreased. At higher temperatures, pH and glucose concentration decreased, but oxidative activity and oocyte maturation rate increased. Lactate concentration and immature oocyte ROS production increased as storage time and temperature increased. The ovary storage for longer than 2 h at 25˚C and 35˚C resulted in low pH of the follicular fluid and high ROS level in immature oocytes. Such conditions seem to damage oocytes and impair their meiotic competence. A decrease in the oxidative activity caused by long time and/or low storage temperature may imply a decrease in oocyte vitality. In conclusion, in the porcine species, the transport of ovaries at 25˚C and 35˚C for 2 h are the best conditions to maintain adequate oocyte quality, meiotic competence and in vitro fertilization rates.
Fil: Tellado, Matías Nicolás. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarez, Gabriel Martín. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dalvit, Gabriel Carlos. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Cetica, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Ovary storage
Oocyte quality
Pig
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30167
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The Conditions of Ovary Storage Affect the Quality of Porcine OocytesTellado, Matías NicolásAlvarez, Gabriel MartínDalvit, Gabriel CarlosCetica, Pablo DanielOvary storageOocyte qualityPighttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4We studied the effect of different storage conditions of porcine ovaries (time, temperature) on the characteristics of the follicular fluid, immature oocyte quality, meiotic competence and in vitro fertilization of oocytes. Ovaries were stored for 2, 4 or 6 h at 3 different temperatures (15˚C, 25˚C or 35˚C). As the storage time increased, pH and glucose concentration of the follicular fluid, percentage of live immature oocytes at germinal vesicle stage, oxidative activity and maturation rate decreased. At higher temperatures, pH and glucose concentration decreased, but oxidative activity and oocyte maturation rate increased. Lactate concentration and immature oocyte ROS production increased as storage time and temperature increased. The ovary storage for longer than 2 h at 25˚C and 35˚C resulted in low pH of the follicular fluid and high ROS level in immature oocytes. Such conditions seem to damage oocytes and impair their meiotic competence. A decrease in the oxidative activity caused by long time and/or low storage temperature may imply a decrease in oocyte vitality. In conclusion, in the porcine species, the transport of ovaries at 25˚C and 35˚C for 2 h are the best conditions to maintain adequate oocyte quality, meiotic competence and in vitro fertilization rates.Fil: Tellado, Matías Nicolás. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alvarez, Gabriel Martín. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dalvit, Gabriel Carlos. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaFil: Cetica, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaScientific Research Publishing2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30167Tellado, Matías Nicolás; Alvarez, Gabriel Martín; Dalvit, Gabriel Carlos; Cetica, Pablo Daniel; The Conditions of Ovary Storage Affect the Quality of Porcine Oocytes; Scientific Research Publishing; Advances in Reproductive Sciences; 2; 3; 8-2014; 57-67; 489132330-0744CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://file.scirp.org/Html/2-1280027_48913.htminfo:eu-repo/semantics/altIdentifier/doi/10.4236/arsci.2014.23007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:17:32Zoai:ri.conicet.gov.ar:11336/30167instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:17:32.56CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Conditions of Ovary Storage Affect the Quality of Porcine Oocytes
title The Conditions of Ovary Storage Affect the Quality of Porcine Oocytes
spellingShingle The Conditions of Ovary Storage Affect the Quality of Porcine Oocytes
Tellado, Matías Nicolás
Ovary storage
Oocyte quality
Pig
title_short The Conditions of Ovary Storage Affect the Quality of Porcine Oocytes
title_full The Conditions of Ovary Storage Affect the Quality of Porcine Oocytes
title_fullStr The Conditions of Ovary Storage Affect the Quality of Porcine Oocytes
title_full_unstemmed The Conditions of Ovary Storage Affect the Quality of Porcine Oocytes
title_sort The Conditions of Ovary Storage Affect the Quality of Porcine Oocytes
dc.creator.none.fl_str_mv Tellado, Matías Nicolás
Alvarez, Gabriel Martín
Dalvit, Gabriel Carlos
Cetica, Pablo Daniel
author Tellado, Matías Nicolás
author_facet Tellado, Matías Nicolás
Alvarez, Gabriel Martín
Dalvit, Gabriel Carlos
Cetica, Pablo Daniel
author_role author
author2 Alvarez, Gabriel Martín
Dalvit, Gabriel Carlos
Cetica, Pablo Daniel
author2_role author
author
author
dc.subject.none.fl_str_mv Ovary storage
Oocyte quality
Pig
topic Ovary storage
Oocyte quality
Pig
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv We studied the effect of different storage conditions of porcine ovaries (time, temperature) on the characteristics of the follicular fluid, immature oocyte quality, meiotic competence and in vitro fertilization of oocytes. Ovaries were stored for 2, 4 or 6 h at 3 different temperatures (15˚C, 25˚C or 35˚C). As the storage time increased, pH and glucose concentration of the follicular fluid, percentage of live immature oocytes at germinal vesicle stage, oxidative activity and maturation rate decreased. At higher temperatures, pH and glucose concentration decreased, but oxidative activity and oocyte maturation rate increased. Lactate concentration and immature oocyte ROS production increased as storage time and temperature increased. The ovary storage for longer than 2 h at 25˚C and 35˚C resulted in low pH of the follicular fluid and high ROS level in immature oocytes. Such conditions seem to damage oocytes and impair their meiotic competence. A decrease in the oxidative activity caused by long time and/or low storage temperature may imply a decrease in oocyte vitality. In conclusion, in the porcine species, the transport of ovaries at 25˚C and 35˚C for 2 h are the best conditions to maintain adequate oocyte quality, meiotic competence and in vitro fertilization rates.
Fil: Tellado, Matías Nicolás. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarez, Gabriel Martín. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dalvit, Gabriel Carlos. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Cetica, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description We studied the effect of different storage conditions of porcine ovaries (time, temperature) on the characteristics of the follicular fluid, immature oocyte quality, meiotic competence and in vitro fertilization of oocytes. Ovaries were stored for 2, 4 or 6 h at 3 different temperatures (15˚C, 25˚C or 35˚C). As the storage time increased, pH and glucose concentration of the follicular fluid, percentage of live immature oocytes at germinal vesicle stage, oxidative activity and maturation rate decreased. At higher temperatures, pH and glucose concentration decreased, but oxidative activity and oocyte maturation rate increased. Lactate concentration and immature oocyte ROS production increased as storage time and temperature increased. The ovary storage for longer than 2 h at 25˚C and 35˚C resulted in low pH of the follicular fluid and high ROS level in immature oocytes. Such conditions seem to damage oocytes and impair their meiotic competence. A decrease in the oxidative activity caused by long time and/or low storage temperature may imply a decrease in oocyte vitality. In conclusion, in the porcine species, the transport of ovaries at 25˚C and 35˚C for 2 h are the best conditions to maintain adequate oocyte quality, meiotic competence and in vitro fertilization rates.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30167
Tellado, Matías Nicolás; Alvarez, Gabriel Martín; Dalvit, Gabriel Carlos; Cetica, Pablo Daniel; The Conditions of Ovary Storage Affect the Quality of Porcine Oocytes; Scientific Research Publishing; Advances in Reproductive Sciences; 2; 3; 8-2014; 57-67; 48913
2330-0744
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30167
identifier_str_mv Tellado, Matías Nicolás; Alvarez, Gabriel Martín; Dalvit, Gabriel Carlos; Cetica, Pablo Daniel; The Conditions of Ovary Storage Affect the Quality of Porcine Oocytes; Scientific Research Publishing; Advances in Reproductive Sciences; 2; 3; 8-2014; 57-67; 48913
2330-0744
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://file.scirp.org/Html/2-1280027_48913.htm
info:eu-repo/semantics/altIdentifier/doi/10.4236/arsci.2014.23007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific Research Publishing
publisher.none.fl_str_mv Scientific Research Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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