Microevolution rather than large genome divergence determines the effectiveness of legume-rhizobia symbiotic interaction under field conditions

Autores
Jozefkowicz, Cintia; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Puente, Mariana; Piccinetti, Carlos; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Despite the vast screening for natural nitrogen-fixing isolates by public and privateconsortia, no significant progresses in the production of improved nitrogen-fixinginoculants for alfalfa production have been made in the last years. Here, we present acomprehensive characterization of the nitrogen-fixing strain Ensifer meliloti B399(originally named Rhizobium meliloti 102F34), probably the inoculant most widely usedin alfalfa production since the 1960s. Complete nucleotide sequence and genomeanalysis of strain B399 showed that the three replicons present in this commercialstrain and the model bacterium Ensifer meliloti 1021 are extremely similar to eachother in terms of nucleotide identity and synteny conservation. In contrast to thatobserved in B399-treated plants, inoculation of plants with strain 1021 did not improvenitrogen content in different alfalfa cultivars under field conditions, suggesting that asmall genomic divergence can drastically impact on the symbiotic phenotype.Therefore, in addition to the traditional screening of natural nitrogen-fixing isolates, thegenome engineering of model strains could be an attractive strategy to improvenitrogen fixation in legume crops.
Fil: Jozefkowicz, Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Frare, Romina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Stritzler, Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Puente, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Piccinetti, Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Materia
Microevolution
Rhizobia
Legumes
Commercial Inoculants
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41143
Acceder
id CONICETDig_4f538eb98de7915e5a8c62238f8dabfa
oai_identifier_str oai:ri.conicet.gov.ar:11336/41143
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Microevolution rather than large genome divergence determines the effectiveness of legume-rhizobia symbiotic interaction under field conditionsJozefkowicz, CintiaBrambilla, Silvina MaricelFrare, Romina AlejandraStritzler, MargaritaPuente, MarianaPiccinetti, CarlosSoto, Gabriela CynthiaAyub, Nicolás DanielMicroevolutionRhizobiaLegumesCommercial Inoculantshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Despite the vast screening for natural nitrogen-fixing isolates by public and privateconsortia, no significant progresses in the production of improved nitrogen-fixinginoculants for alfalfa production have been made in the last years. Here, we present acomprehensive characterization of the nitrogen-fixing strain Ensifer meliloti B399(originally named Rhizobium meliloti 102F34), probably the inoculant most widely usedin alfalfa production since the 1960s. Complete nucleotide sequence and genomeanalysis of strain B399 showed that the three replicons present in this commercialstrain and the model bacterium Ensifer meliloti 1021 are extremely similar to eachother in terms of nucleotide identity and synteny conservation. In contrast to thatobserved in B399-treated plants, inoculation of plants with strain 1021 did not improvenitrogen content in different alfalfa cultivars under field conditions, suggesting that asmall genomic divergence can drastically impact on the symbiotic phenotype.Therefore, in addition to the traditional screening of natural nitrogen-fixing isolates, thegenome engineering of model strains could be an attractive strategy to improvenitrogen fixation in legume crops.Fil: Jozefkowicz, Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaFil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaFil: Frare, Romina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaFil: Stritzler, Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaFil: Puente, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Piccinetti, Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaFil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaSpringer2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41143Jozefkowicz, Cintia; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Puente, Mariana; et al.; Microevolution rather than large genome divergence determines the effectiveness of legume-rhizobia symbiotic interaction under field conditions; Springer; Journal Of Molecular Evolution; 85; 3-4; 8-2017; 79-830022-2844CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00239-017-9808-6info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00239-017-9808-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:29Zoai:ri.conicet.gov.ar:11336/41143instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:30.283CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Microevolution rather than large genome divergence determines the effectiveness of legume-rhizobia symbiotic interaction under field conditions
title Microevolution rather than large genome divergence determines the effectiveness of legume-rhizobia symbiotic interaction under field conditions
spellingShingle Microevolution rather than large genome divergence determines the effectiveness of legume-rhizobia symbiotic interaction under field conditions
Jozefkowicz, Cintia
Microevolution
Rhizobia
Legumes
Commercial Inoculants
title_short Microevolution rather than large genome divergence determines the effectiveness of legume-rhizobia symbiotic interaction under field conditions
title_full Microevolution rather than large genome divergence determines the effectiveness of legume-rhizobia symbiotic interaction under field conditions
title_fullStr Microevolution rather than large genome divergence determines the effectiveness of legume-rhizobia symbiotic interaction under field conditions
title_full_unstemmed Microevolution rather than large genome divergence determines the effectiveness of legume-rhizobia symbiotic interaction under field conditions
title_sort Microevolution rather than large genome divergence determines the effectiveness of legume-rhizobia symbiotic interaction under field conditions
dc.creator.none.fl_str_mv Jozefkowicz, Cintia
Brambilla, Silvina Maricel
Frare, Romina Alejandra
Stritzler, Margarita
Puente, Mariana
Piccinetti, Carlos
Soto, Gabriela Cynthia
Ayub, Nicolás Daniel
author Jozefkowicz, Cintia
author_facet Jozefkowicz, Cintia
Brambilla, Silvina Maricel
Frare, Romina Alejandra
Stritzler, Margarita
Puente, Mariana
Piccinetti, Carlos
Soto, Gabriela Cynthia
Ayub, Nicolás Daniel
author_role author
author2 Brambilla, Silvina Maricel
Frare, Romina Alejandra
Stritzler, Margarita
Puente, Mariana
Piccinetti, Carlos
Soto, Gabriela Cynthia
Ayub, Nicolás Daniel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Microevolution
Rhizobia
Legumes
Commercial Inoculants
topic Microevolution
Rhizobia
Legumes
Commercial Inoculants
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Despite the vast screening for natural nitrogen-fixing isolates by public and privateconsortia, no significant progresses in the production of improved nitrogen-fixinginoculants for alfalfa production have been made in the last years. Here, we present acomprehensive characterization of the nitrogen-fixing strain Ensifer meliloti B399(originally named Rhizobium meliloti 102F34), probably the inoculant most widely usedin alfalfa production since the 1960s. Complete nucleotide sequence and genomeanalysis of strain B399 showed that the three replicons present in this commercialstrain and the model bacterium Ensifer meliloti 1021 are extremely similar to eachother in terms of nucleotide identity and synteny conservation. In contrast to thatobserved in B399-treated plants, inoculation of plants with strain 1021 did not improvenitrogen content in different alfalfa cultivars under field conditions, suggesting that asmall genomic divergence can drastically impact on the symbiotic phenotype.Therefore, in addition to the traditional screening of natural nitrogen-fixing isolates, thegenome engineering of model strains could be an attractive strategy to improvenitrogen fixation in legume crops.
Fil: Jozefkowicz, Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Frare, Romina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Stritzler, Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Puente, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Piccinetti, Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
description Despite the vast screening for natural nitrogen-fixing isolates by public and privateconsortia, no significant progresses in the production of improved nitrogen-fixinginoculants for alfalfa production have been made in the last years. Here, we present acomprehensive characterization of the nitrogen-fixing strain Ensifer meliloti B399(originally named Rhizobium meliloti 102F34), probably the inoculant most widely usedin alfalfa production since the 1960s. Complete nucleotide sequence and genomeanalysis of strain B399 showed that the three replicons present in this commercialstrain and the model bacterium Ensifer meliloti 1021 are extremely similar to eachother in terms of nucleotide identity and synteny conservation. In contrast to thatobserved in B399-treated plants, inoculation of plants with strain 1021 did not improvenitrogen content in different alfalfa cultivars under field conditions, suggesting that asmall genomic divergence can drastically impact on the symbiotic phenotype.Therefore, in addition to the traditional screening of natural nitrogen-fixing isolates, thegenome engineering of model strains could be an attractive strategy to improvenitrogen fixation in legume crops.
publishDate 2017
dc.date.none.fl_str_mv 2017-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41143
Jozefkowicz, Cintia; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Puente, Mariana; et al.; Microevolution rather than large genome divergence determines the effectiveness of legume-rhizobia symbiotic interaction under field conditions; Springer; Journal Of Molecular Evolution; 85; 3-4; 8-2017; 79-83
0022-2844
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41143
identifier_str_mv Jozefkowicz, Cintia; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Puente, Mariana; et al.; Microevolution rather than large genome divergence determines the effectiveness of legume-rhizobia symbiotic interaction under field conditions; Springer; Journal Of Molecular Evolution; 85; 3-4; 8-2017; 79-83
0022-2844
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00239-017-9808-6
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00239-017-9808-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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