The XY Body of the Cat (Felis catus): Structural Differentiations and Protein Immunolocalization

Autores
Sciurano, Roberta Beatriz; De Luca, Geraldine; Rahn, Irene Monica; Solari, Alberto Juan
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The heteromorphic X and Y chromosomes behave in a special way in mammalian spermatocytes; they form the XY body and synapse only partially. The aim of this article was to study the origin and the role of the special differentiations in the XY pair of the domestic cat during pachytene by analyzing its fine structural characteristics and the immunolocalization of the main meiotic proteins SYCP3, SYCP1, SYCE3, SMC3, γ-H2AX, BRCA1, H3K27me3, and MLH1. The cat XY body shows particularly striking structures: an extreme degree of axial fibrillation in late pachynema and a special location of SYCP3-containing fibrils, bridging different regions of the main X axis, as well as one bridge at the inner end of the pairing region that colocalizes with the single mandatory MLH1 focus. There are sequential changes, first bullous expansions, then subdivision into fibrils, all involving axial thickening. The chromatin of the XY body presents the usual features of meiotic sex chromosome inactivation. An analysis of the XY body of many eutherians and metatherians suggests that axial thickenings are primitive features. The sequential changes in the mass and location of SYCP3-containing fibers vary among the clades because of specific processes of axial assembly/disassembly occurring in different species.
Fil: Sciurano, Roberta Beatriz. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Luca, Geraldine. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Rahn, Irene Monica. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Solari, Alberto Juan. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Xy Body
Meiosis
Synaptonemal Complex
Protein Markers
Cats
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47726
Acceder
id CONICETDig_4f402b6cdbacd2414cb58c5318b2f6fa
oai_identifier_str oai:ri.conicet.gov.ar:11336/47726
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The XY Body of the Cat (Felis catus): Structural Differentiations and Protein ImmunolocalizationSciurano, Roberta BeatrizDe Luca, GeraldineRahn, Irene MonicaSolari, Alberto JuanXy BodyMeiosisSynaptonemal ComplexProtein MarkersCatshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The heteromorphic X and Y chromosomes behave in a special way in mammalian spermatocytes; they form the XY body and synapse only partially. The aim of this article was to study the origin and the role of the special differentiations in the XY pair of the domestic cat during pachytene by analyzing its fine structural characteristics and the immunolocalization of the main meiotic proteins SYCP3, SYCP1, SYCE3, SMC3, γ-H2AX, BRCA1, H3K27me3, and MLH1. The cat XY body shows particularly striking structures: an extreme degree of axial fibrillation in late pachynema and a special location of SYCP3-containing fibrils, bridging different regions of the main X axis, as well as one bridge at the inner end of the pairing region that colocalizes with the single mandatory MLH1 focus. There are sequential changes, first bullous expansions, then subdivision into fibrils, all involving axial thickening. The chromatin of the XY body presents the usual features of meiotic sex chromosome inactivation. An analysis of the XY body of many eutherians and metatherians suggests that axial thickenings are primitive features. The sequential changes in the mass and location of SYCP3-containing fibers vary among the clades because of specific processes of axial assembly/disassembly occurring in different species.Fil: Sciurano, Roberta Beatriz. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: De Luca, Geraldine. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Rahn, Irene Monica. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Solari, Alberto Juan. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaKarger2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47726Sciurano, Roberta Beatriz; De Luca, Geraldine; Rahn, Irene Monica; Solari, Alberto Juan; The XY Body of the Cat (Felis catus): Structural Differentiations and Protein Immunolocalization; Karger; Cytogenetic And Genome Research; 152; 3; 8-2017; 137-1471424-8581CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159/000479569info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/479569info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:03:26Zoai:ri.conicet.gov.ar:11336/47726instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:03:26.721CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The XY Body of the Cat (Felis catus): Structural Differentiations and Protein Immunolocalization
title The XY Body of the Cat (Felis catus): Structural Differentiations and Protein Immunolocalization
spellingShingle The XY Body of the Cat (Felis catus): Structural Differentiations and Protein Immunolocalization
Sciurano, Roberta Beatriz
Xy Body
Meiosis
Synaptonemal Complex
Protein Markers
Cats
title_short The XY Body of the Cat (Felis catus): Structural Differentiations and Protein Immunolocalization
title_full The XY Body of the Cat (Felis catus): Structural Differentiations and Protein Immunolocalization
title_fullStr The XY Body of the Cat (Felis catus): Structural Differentiations and Protein Immunolocalization
title_full_unstemmed The XY Body of the Cat (Felis catus): Structural Differentiations and Protein Immunolocalization
title_sort The XY Body of the Cat (Felis catus): Structural Differentiations and Protein Immunolocalization
dc.creator.none.fl_str_mv Sciurano, Roberta Beatriz
De Luca, Geraldine
Rahn, Irene Monica
Solari, Alberto Juan
author Sciurano, Roberta Beatriz
author_facet Sciurano, Roberta Beatriz
De Luca, Geraldine
Rahn, Irene Monica
Solari, Alberto Juan
author_role author
author2 De Luca, Geraldine
Rahn, Irene Monica
Solari, Alberto Juan
author2_role author
author
author
dc.subject.none.fl_str_mv Xy Body
Meiosis
Synaptonemal Complex
Protein Markers
Cats
topic Xy Body
Meiosis
Synaptonemal Complex
Protein Markers
Cats
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The heteromorphic X and Y chromosomes behave in a special way in mammalian spermatocytes; they form the XY body and synapse only partially. The aim of this article was to study the origin and the role of the special differentiations in the XY pair of the domestic cat during pachytene by analyzing its fine structural characteristics and the immunolocalization of the main meiotic proteins SYCP3, SYCP1, SYCE3, SMC3, γ-H2AX, BRCA1, H3K27me3, and MLH1. The cat XY body shows particularly striking structures: an extreme degree of axial fibrillation in late pachynema and a special location of SYCP3-containing fibrils, bridging different regions of the main X axis, as well as one bridge at the inner end of the pairing region that colocalizes with the single mandatory MLH1 focus. There are sequential changes, first bullous expansions, then subdivision into fibrils, all involving axial thickening. The chromatin of the XY body presents the usual features of meiotic sex chromosome inactivation. An analysis of the XY body of many eutherians and metatherians suggests that axial thickenings are primitive features. The sequential changes in the mass and location of SYCP3-containing fibers vary among the clades because of specific processes of axial assembly/disassembly occurring in different species.
Fil: Sciurano, Roberta Beatriz. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Luca, Geraldine. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Rahn, Irene Monica. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Solari, Alberto Juan. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The heteromorphic X and Y chromosomes behave in a special way in mammalian spermatocytes; they form the XY body and synapse only partially. The aim of this article was to study the origin and the role of the special differentiations in the XY pair of the domestic cat during pachytene by analyzing its fine structural characteristics and the immunolocalization of the main meiotic proteins SYCP3, SYCP1, SYCE3, SMC3, γ-H2AX, BRCA1, H3K27me3, and MLH1. The cat XY body shows particularly striking structures: an extreme degree of axial fibrillation in late pachynema and a special location of SYCP3-containing fibrils, bridging different regions of the main X axis, as well as one bridge at the inner end of the pairing region that colocalizes with the single mandatory MLH1 focus. There are sequential changes, first bullous expansions, then subdivision into fibrils, all involving axial thickening. The chromatin of the XY body presents the usual features of meiotic sex chromosome inactivation. An analysis of the XY body of many eutherians and metatherians suggests that axial thickenings are primitive features. The sequential changes in the mass and location of SYCP3-containing fibers vary among the clades because of specific processes of axial assembly/disassembly occurring in different species.
publishDate 2017
dc.date.none.fl_str_mv 2017-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47726
Sciurano, Roberta Beatriz; De Luca, Geraldine; Rahn, Irene Monica; Solari, Alberto Juan; The XY Body of the Cat (Felis catus): Structural Differentiations and Protein Immunolocalization; Karger; Cytogenetic And Genome Research; 152; 3; 8-2017; 137-147
1424-8581
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47726
identifier_str_mv Sciurano, Roberta Beatriz; De Luca, Geraldine; Rahn, Irene Monica; Solari, Alberto Juan; The XY Body of the Cat (Felis catus): Structural Differentiations and Protein Immunolocalization; Karger; Cytogenetic And Genome Research; 152; 3; 8-2017; 137-147
1424-8581
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159/000479569
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/479569
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781267044990976
score 12.982451

Publicaciones similares