Group 2 innate lymphoid cells exhibit progressively higher levels of activation during worsening of liver fibrosis

Autores
Gonzalez Polo, Virginia; Pucci Molineris, Melisa Eliana; Cervera, Victorio; Gambaro, Sabrina Eliana; Yantorno, Silvina E.; Descalzi, Valeria; Tiribelli, Claudio; Gondolesi, Gabriel Eduardo; Meier, Dominik
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: The interleukin-33/interleukin-13 pathway is involved in the immunopathology of liver fibrosis and recently characterized group 2 innate lymphoid cells (ILC2) were identified as profibrotic immune cells in the liver of mouse models. Our aim was to elucidate whether ILC2 might be present in human liver tissue and whether ILC2 contribute to liver fibrosis. Materials and methods: To identify ILC2 in liver tissue and blood, we purified mononuclear immune cells from needle biopsies, cirrhotic explant specimen, and paired peripheral blood samples. Cell suspensions were incubated with specific markers for ILC2 and analyzed by flow cytometry. The CD69 marker was included to assess the activation level of ILC2. In addition, we determined the IL-33 plasma level. Results: Results were correlated with the METAVIR fibrotic score of patients enrolled in this study. We detected ILC2 in a higher percentage of CD45+ cells in liver tissue than in paired peripheral blood. The number of ILC2 was significantly increased in fibrotic tissue, but only slightly increased in paired peripheral blood. A higher percentage of CD69+ ILC2 was observed in fibrotic tissue, and this increase correlates positively with aggravation of liver fibrosis measured by fibrotic METAVIR score. A higher level of plasma IL-33 was only detected in samples obtained from cirrhotic patients. Conclusion: Our study indicates that ILC2 are present in the human liver and are activated in tissue contributing to the immunopathology of human liver fibrosis, independently ofthe etiology; which might be a potential new therapeutic target.
Fil: Gonzalez Polo, Virginia. Universidad Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina
Fil: Pucci Molineris, Melisa Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Universidad Favaloro; Argentina
Fil: Cervera, Victorio. Universidad Favaloro; Argentina
Fil: Gambaro, Sabrina Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Universidad Favaloro; Argentina
Fil: Yantorno, Silvina E.. Fundación Favaloro; Argentina
Fil: Descalzi, Valeria. Fundación Favaloro; Argentina
Fil: Tiribelli, Claudio. Italian Liver Foundation; Italia
Fil: Gondolesi, Gabriel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Universidad Favaloro; Argentina
Fil: Meier, Dominik. Universidad Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina
Materia
AUTOIMMUNE HEPATITIS
ILC2
LIVER DISEASE
STEATOHEPATITIS
VIRAL HEPATITIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/113176
Acceder
id CONICETDig_4ef3b341585653f7ed38becd6123be2b
oai_identifier_str oai:ri.conicet.gov.ar:11336/113176
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Group 2 innate lymphoid cells exhibit progressively higher levels of activation during worsening of liver fibrosisGonzalez Polo, VirginiaPucci Molineris, Melisa ElianaCervera, VictorioGambaro, Sabrina ElianaYantorno, Silvina E.Descalzi, ValeriaTiribelli, ClaudioGondolesi, Gabriel EduardoMeier, DominikAUTOIMMUNE HEPATITISILC2LIVER DISEASESTEATOHEPATITISVIRAL HEPATITIShttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Introduction: The interleukin-33/interleukin-13 pathway is involved in the immunopathology of liver fibrosis and recently characterized group 2 innate lymphoid cells (ILC2) were identified as profibrotic immune cells in the liver of mouse models. Our aim was to elucidate whether ILC2 might be present in human liver tissue and whether ILC2 contribute to liver fibrosis. Materials and methods: To identify ILC2 in liver tissue and blood, we purified mononuclear immune cells from needle biopsies, cirrhotic explant specimen, and paired peripheral blood samples. Cell suspensions were incubated with specific markers for ILC2 and analyzed by flow cytometry. The CD69 marker was included to assess the activation level of ILC2. In addition, we determined the IL-33 plasma level. Results: Results were correlated with the METAVIR fibrotic score of patients enrolled in this study. We detected ILC2 in a higher percentage of CD45+ cells in liver tissue than in paired peripheral blood. The number of ILC2 was significantly increased in fibrotic tissue, but only slightly increased in paired peripheral blood. A higher percentage of CD69+ ILC2 was observed in fibrotic tissue, and this increase correlates positively with aggravation of liver fibrosis measured by fibrotic METAVIR score. A higher level of plasma IL-33 was only detected in samples obtained from cirrhotic patients. Conclusion: Our study indicates that ILC2 are present in the human liver and are activated in tissue contributing to the immunopathology of human liver fibrosis, independently ofthe etiology; which might be a potential new therapeutic target.Fil: Gonzalez Polo, Virginia. Universidad Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; ArgentinaFil: Pucci Molineris, Melisa Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Universidad Favaloro; ArgentinaFil: Cervera, Victorio. Universidad Favaloro; ArgentinaFil: Gambaro, Sabrina Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Universidad Favaloro; ArgentinaFil: Yantorno, Silvina E.. Fundación Favaloro; ArgentinaFil: Descalzi, Valeria. Fundación Favaloro; ArgentinaFil: Tiribelli, Claudio. Italian Liver Foundation; ItaliaFil: Gondolesi, Gabriel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Universidad Favaloro; ArgentinaFil: Meier, Dominik. Universidad Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; ArgentinaMexican Association of Hepatology2019-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113176Gonzalez Polo, Virginia; Pucci Molineris, Melisa Eliana; Cervera, Victorio; Gambaro, Sabrina Eliana; Yantorno, Silvina E.; et al.; Group 2 innate lymphoid cells exhibit progressively higher levels of activation during worsening of liver fibrosis; Mexican Association of Hepatology; Annals of Hepatology; 18; 2; 3-2019; 366-3721665-2681CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S166526811930016Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.aohep.2018.12.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:33Zoai:ri.conicet.gov.ar:11336/113176instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:34.275CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Group 2 innate lymphoid cells exhibit progressively higher levels of activation during worsening of liver fibrosis
title Group 2 innate lymphoid cells exhibit progressively higher levels of activation during worsening of liver fibrosis
spellingShingle Group 2 innate lymphoid cells exhibit progressively higher levels of activation during worsening of liver fibrosis
Gonzalez Polo, Virginia
AUTOIMMUNE HEPATITIS
ILC2
LIVER DISEASE
STEATOHEPATITIS
VIRAL HEPATITIS
title_short Group 2 innate lymphoid cells exhibit progressively higher levels of activation during worsening of liver fibrosis
title_full Group 2 innate lymphoid cells exhibit progressively higher levels of activation during worsening of liver fibrosis
title_fullStr Group 2 innate lymphoid cells exhibit progressively higher levels of activation during worsening of liver fibrosis
title_full_unstemmed Group 2 innate lymphoid cells exhibit progressively higher levels of activation during worsening of liver fibrosis
title_sort Group 2 innate lymphoid cells exhibit progressively higher levels of activation during worsening of liver fibrosis
dc.creator.none.fl_str_mv Gonzalez Polo, Virginia
Pucci Molineris, Melisa Eliana
Cervera, Victorio
Gambaro, Sabrina Eliana
Yantorno, Silvina E.
Descalzi, Valeria
Tiribelli, Claudio
Gondolesi, Gabriel Eduardo
Meier, Dominik
author Gonzalez Polo, Virginia
author_facet Gonzalez Polo, Virginia
Pucci Molineris, Melisa Eliana
Cervera, Victorio
Gambaro, Sabrina Eliana
Yantorno, Silvina E.
Descalzi, Valeria
Tiribelli, Claudio
Gondolesi, Gabriel Eduardo
Meier, Dominik
author_role author
author2 Pucci Molineris, Melisa Eliana
Cervera, Victorio
Gambaro, Sabrina Eliana
Yantorno, Silvina E.
Descalzi, Valeria
Tiribelli, Claudio
Gondolesi, Gabriel Eduardo
Meier, Dominik
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AUTOIMMUNE HEPATITIS
ILC2
LIVER DISEASE
STEATOHEPATITIS
VIRAL HEPATITIS
topic AUTOIMMUNE HEPATITIS
ILC2
LIVER DISEASE
STEATOHEPATITIS
VIRAL HEPATITIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Introduction: The interleukin-33/interleukin-13 pathway is involved in the immunopathology of liver fibrosis and recently characterized group 2 innate lymphoid cells (ILC2) were identified as profibrotic immune cells in the liver of mouse models. Our aim was to elucidate whether ILC2 might be present in human liver tissue and whether ILC2 contribute to liver fibrosis. Materials and methods: To identify ILC2 in liver tissue and blood, we purified mononuclear immune cells from needle biopsies, cirrhotic explant specimen, and paired peripheral blood samples. Cell suspensions were incubated with specific markers for ILC2 and analyzed by flow cytometry. The CD69 marker was included to assess the activation level of ILC2. In addition, we determined the IL-33 plasma level. Results: Results were correlated with the METAVIR fibrotic score of patients enrolled in this study. We detected ILC2 in a higher percentage of CD45+ cells in liver tissue than in paired peripheral blood. The number of ILC2 was significantly increased in fibrotic tissue, but only slightly increased in paired peripheral blood. A higher percentage of CD69+ ILC2 was observed in fibrotic tissue, and this increase correlates positively with aggravation of liver fibrosis measured by fibrotic METAVIR score. A higher level of plasma IL-33 was only detected in samples obtained from cirrhotic patients. Conclusion: Our study indicates that ILC2 are present in the human liver and are activated in tissue contributing to the immunopathology of human liver fibrosis, independently ofthe etiology; which might be a potential new therapeutic target.
Fil: Gonzalez Polo, Virginia. Universidad Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina
Fil: Pucci Molineris, Melisa Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Universidad Favaloro; Argentina
Fil: Cervera, Victorio. Universidad Favaloro; Argentina
Fil: Gambaro, Sabrina Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Universidad Favaloro; Argentina
Fil: Yantorno, Silvina E.. Fundación Favaloro; Argentina
Fil: Descalzi, Valeria. Fundación Favaloro; Argentina
Fil: Tiribelli, Claudio. Italian Liver Foundation; Italia
Fil: Gondolesi, Gabriel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Universidad Favaloro; Argentina
Fil: Meier, Dominik. Universidad Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina
description Introduction: The interleukin-33/interleukin-13 pathway is involved in the immunopathology of liver fibrosis and recently characterized group 2 innate lymphoid cells (ILC2) were identified as profibrotic immune cells in the liver of mouse models. Our aim was to elucidate whether ILC2 might be present in human liver tissue and whether ILC2 contribute to liver fibrosis. Materials and methods: To identify ILC2 in liver tissue and blood, we purified mononuclear immune cells from needle biopsies, cirrhotic explant specimen, and paired peripheral blood samples. Cell suspensions were incubated with specific markers for ILC2 and analyzed by flow cytometry. The CD69 marker was included to assess the activation level of ILC2. In addition, we determined the IL-33 plasma level. Results: Results were correlated with the METAVIR fibrotic score of patients enrolled in this study. We detected ILC2 in a higher percentage of CD45+ cells in liver tissue than in paired peripheral blood. The number of ILC2 was significantly increased in fibrotic tissue, but only slightly increased in paired peripheral blood. A higher percentage of CD69+ ILC2 was observed in fibrotic tissue, and this increase correlates positively with aggravation of liver fibrosis measured by fibrotic METAVIR score. A higher level of plasma IL-33 was only detected in samples obtained from cirrhotic patients. Conclusion: Our study indicates that ILC2 are present in the human liver and are activated in tissue contributing to the immunopathology of human liver fibrosis, independently ofthe etiology; which might be a potential new therapeutic target.
publishDate 2019
dc.date.none.fl_str_mv 2019-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/113176
Gonzalez Polo, Virginia; Pucci Molineris, Melisa Eliana; Cervera, Victorio; Gambaro, Sabrina Eliana; Yantorno, Silvina E.; et al.; Group 2 innate lymphoid cells exhibit progressively higher levels of activation during worsening of liver fibrosis; Mexican Association of Hepatology; Annals of Hepatology; 18; 2; 3-2019; 366-372
1665-2681
CONICET Digital
CONICET
url http://hdl.handle.net/11336/113176
identifier_str_mv Gonzalez Polo, Virginia; Pucci Molineris, Melisa Eliana; Cervera, Victorio; Gambaro, Sabrina Eliana; Yantorno, Silvina E.; et al.; Group 2 innate lymphoid cells exhibit progressively higher levels of activation during worsening of liver fibrosis; Mexican Association of Hepatology; Annals of Hepatology; 18; 2; 3-2019; 366-372
1665-2681
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S166526811930016X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aohep.2018.12.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mexican Association of Hepatology
publisher.none.fl_str_mv Mexican Association of Hepatology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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