Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: The use of different inoculation techniques and their effects on the tomato leafminer Tuta abso...

Autores
Allegrucci, Natalia; Velázquez, María Silvana; Russo, Maria Leticia; Pérez, María Emilia; Scorsetti, Ana Clara
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fungal entomopathogens can naturally regulate populations of various insects. The entomopathogen Beauveria bassiana (Bals.- Criv.) Vuill. is also able to endophytically colonize different plants. Endophytic colonization by entomopathogens may provide a source of indirect interactions between fungi and insects and has been associated with the ability of the fungus to control insect pests. The tomato leaf miner, Tuta absoluta (Meyrick) is considered one of the most devastating pests of tomato (Solanum lycopersicum L.) and its difficult control is due to its miner habit, short life cycle, and high reproductive capacity. The aims of this study were: (i) to use three different techniques, i.e. leaf spraying, seed immersion and root dipping, for the endophytic inoculation of B. bassiana in tomato plants; and (ii) to assess the effect of B. bassiana on tomato leaf consumption and mortality of T. absoluta after inoculation. The percentage of colonization by B. bassiana was assessed 7, 14 and 28 days after inoculation. All inoculation techniques employed allowed the recovery of B. bassiana, although our results showed significant differences between techniques. Leaf spraying was the most effective, with the highest percentage of colonization recorded 7 days after inoculation. We also evaluated (i) the effect on the mortality of T. absoluta by direct contact with conidia of B. bassiana, and (ii) the effect on tomato leaf consumption and mortality by indirect contact through ingestion of inoculated plant tissues with B. bassiana. Mortality bioassays showed that B. bassiana infected T. absoluta, either by direct contact or indirectly, via ingestion of inoculated tomato leaves. Direct contact showed a higher percentage of mortality and a lower median survival time (MST) than indirect contact. Significant differences in the mortality percentages of T. absoluta after exposure with B. bassiana were found among the treatments and the control. Our results suggest that the endophytic inoculation of B. bassiana in tomato crops provides the basis for further investigation, which should focus on the virulence of the endophytic B. bassiana against T. absoluta.
Fil: Allegrucci, Natalia. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Velázquez, María Silvana. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Russo, Maria Leticia. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Scorsetti, Ana Clara. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
BEAUVERIA BASSIANA
BIOCONTROL
ENDOPHYTES
FUNGAL ENTOMOPATHOGENS
SOLANUM LYCOPERSICUM
TUTA ABSOLUTA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49743
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/49743
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: The use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)Allegrucci, NataliaVelázquez, María SilvanaRusso, Maria LeticiaPérez, María EmiliaScorsetti, Ana ClaraBEAUVERIA BASSIANABIOCONTROLENDOPHYTESFUNGAL ENTOMOPATHOGENSSOLANUM LYCOPERSICUMTUTA ABSOLUTAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fungal entomopathogens can naturally regulate populations of various insects. The entomopathogen Beauveria bassiana (Bals.- Criv.) Vuill. is also able to endophytically colonize different plants. Endophytic colonization by entomopathogens may provide a source of indirect interactions between fungi and insects and has been associated with the ability of the fungus to control insect pests. The tomato leaf miner, Tuta absoluta (Meyrick) is considered one of the most devastating pests of tomato (Solanum lycopersicum L.) and its difficult control is due to its miner habit, short life cycle, and high reproductive capacity. The aims of this study were: (i) to use three different techniques, i.e. leaf spraying, seed immersion and root dipping, for the endophytic inoculation of B. bassiana in tomato plants; and (ii) to assess the effect of B. bassiana on tomato leaf consumption and mortality of T. absoluta after inoculation. The percentage of colonization by B. bassiana was assessed 7, 14 and 28 days after inoculation. All inoculation techniques employed allowed the recovery of B. bassiana, although our results showed significant differences between techniques. Leaf spraying was the most effective, with the highest percentage of colonization recorded 7 days after inoculation. We also evaluated (i) the effect on the mortality of T. absoluta by direct contact with conidia of B. bassiana, and (ii) the effect on tomato leaf consumption and mortality by indirect contact through ingestion of inoculated plant tissues with B. bassiana. Mortality bioassays showed that B. bassiana infected T. absoluta, either by direct contact or indirectly, via ingestion of inoculated tomato leaves. Direct contact showed a higher percentage of mortality and a lower median survival time (MST) than indirect contact. Significant differences in the mortality percentages of T. absoluta after exposure with B. bassiana were found among the treatments and the control. Our results suggest that the endophytic inoculation of B. bassiana in tomato crops provides the basis for further investigation, which should focus on the virulence of the endophytic B. bassiana against T. absoluta.Fil: Allegrucci, Natalia. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Velázquez, María Silvana. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Russo, Maria Leticia. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pérez, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Scorsetti, Ana Clara. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaPolish Academy of Sciences2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49743Allegrucci, Natalia; Velázquez, María Silvana; Russo, Maria Leticia; Pérez, María Emilia; Scorsetti, Ana Clara; Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: The use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae); Polish Academy of Sciences; Journal of Plant Protection Research; 57; 4; 11-2017; 331-3371899-007XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1515/jppr-2017-0045info:eu-repo/semantics/altIdentifier/url/https://content.sciendo.com/view/journals/jppr/ahead-of-print/article-10.1515-jppr-2017-0045.xmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:18Zoai:ri.conicet.gov.ar:11336/49743instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:18.339CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: The use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)
title Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: The use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)
spellingShingle Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: The use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)
Allegrucci, Natalia
BEAUVERIA BASSIANA
BIOCONTROL
ENDOPHYTES
FUNGAL ENTOMOPATHOGENS
SOLANUM LYCOPERSICUM
TUTA ABSOLUTA
title_short Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: The use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)
title_full Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: The use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)
title_fullStr Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: The use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)
title_full_unstemmed Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: The use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)
title_sort Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: The use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)
dc.creator.none.fl_str_mv Allegrucci, Natalia
Velázquez, María Silvana
Russo, Maria Leticia
Pérez, María Emilia
Scorsetti, Ana Clara
author Allegrucci, Natalia
author_facet Allegrucci, Natalia
Velázquez, María Silvana
Russo, Maria Leticia
Pérez, María Emilia
Scorsetti, Ana Clara
author_role author
author2 Velázquez, María Silvana
Russo, Maria Leticia
Pérez, María Emilia
Scorsetti, Ana Clara
author2_role author
author
author
author
dc.subject.none.fl_str_mv BEAUVERIA BASSIANA
BIOCONTROL
ENDOPHYTES
FUNGAL ENTOMOPATHOGENS
SOLANUM LYCOPERSICUM
TUTA ABSOLUTA
topic BEAUVERIA BASSIANA
BIOCONTROL
ENDOPHYTES
FUNGAL ENTOMOPATHOGENS
SOLANUM LYCOPERSICUM
TUTA ABSOLUTA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fungal entomopathogens can naturally regulate populations of various insects. The entomopathogen Beauveria bassiana (Bals.- Criv.) Vuill. is also able to endophytically colonize different plants. Endophytic colonization by entomopathogens may provide a source of indirect interactions between fungi and insects and has been associated with the ability of the fungus to control insect pests. The tomato leaf miner, Tuta absoluta (Meyrick) is considered one of the most devastating pests of tomato (Solanum lycopersicum L.) and its difficult control is due to its miner habit, short life cycle, and high reproductive capacity. The aims of this study were: (i) to use three different techniques, i.e. leaf spraying, seed immersion and root dipping, for the endophytic inoculation of B. bassiana in tomato plants; and (ii) to assess the effect of B. bassiana on tomato leaf consumption and mortality of T. absoluta after inoculation. The percentage of colonization by B. bassiana was assessed 7, 14 and 28 days after inoculation. All inoculation techniques employed allowed the recovery of B. bassiana, although our results showed significant differences between techniques. Leaf spraying was the most effective, with the highest percentage of colonization recorded 7 days after inoculation. We also evaluated (i) the effect on the mortality of T. absoluta by direct contact with conidia of B. bassiana, and (ii) the effect on tomato leaf consumption and mortality by indirect contact through ingestion of inoculated plant tissues with B. bassiana. Mortality bioassays showed that B. bassiana infected T. absoluta, either by direct contact or indirectly, via ingestion of inoculated tomato leaves. Direct contact showed a higher percentage of mortality and a lower median survival time (MST) than indirect contact. Significant differences in the mortality percentages of T. absoluta after exposure with B. bassiana were found among the treatments and the control. Our results suggest that the endophytic inoculation of B. bassiana in tomato crops provides the basis for further investigation, which should focus on the virulence of the endophytic B. bassiana against T. absoluta.
Fil: Allegrucci, Natalia. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Velázquez, María Silvana. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Russo, Maria Leticia. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Scorsetti, Ana Clara. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Fungal entomopathogens can naturally regulate populations of various insects. The entomopathogen Beauveria bassiana (Bals.- Criv.) Vuill. is also able to endophytically colonize different plants. Endophytic colonization by entomopathogens may provide a source of indirect interactions between fungi and insects and has been associated with the ability of the fungus to control insect pests. The tomato leaf miner, Tuta absoluta (Meyrick) is considered one of the most devastating pests of tomato (Solanum lycopersicum L.) and its difficult control is due to its miner habit, short life cycle, and high reproductive capacity. The aims of this study were: (i) to use three different techniques, i.e. leaf spraying, seed immersion and root dipping, for the endophytic inoculation of B. bassiana in tomato plants; and (ii) to assess the effect of B. bassiana on tomato leaf consumption and mortality of T. absoluta after inoculation. The percentage of colonization by B. bassiana was assessed 7, 14 and 28 days after inoculation. All inoculation techniques employed allowed the recovery of B. bassiana, although our results showed significant differences between techniques. Leaf spraying was the most effective, with the highest percentage of colonization recorded 7 days after inoculation. We also evaluated (i) the effect on the mortality of T. absoluta by direct contact with conidia of B. bassiana, and (ii) the effect on tomato leaf consumption and mortality by indirect contact through ingestion of inoculated plant tissues with B. bassiana. Mortality bioassays showed that B. bassiana infected T. absoluta, either by direct contact or indirectly, via ingestion of inoculated tomato leaves. Direct contact showed a higher percentage of mortality and a lower median survival time (MST) than indirect contact. Significant differences in the mortality percentages of T. absoluta after exposure with B. bassiana were found among the treatments and the control. Our results suggest that the endophytic inoculation of B. bassiana in tomato crops provides the basis for further investigation, which should focus on the virulence of the endophytic B. bassiana against T. absoluta.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49743
Allegrucci, Natalia; Velázquez, María Silvana; Russo, Maria Leticia; Pérez, María Emilia; Scorsetti, Ana Clara; Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: The use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae); Polish Academy of Sciences; Journal of Plant Protection Research; 57; 4; 11-2017; 331-337
1899-007X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49743
identifier_str_mv Allegrucci, Natalia; Velázquez, María Silvana; Russo, Maria Leticia; Pérez, María Emilia; Scorsetti, Ana Clara; Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: The use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae); Polish Academy of Sciences; Journal of Plant Protection Research; 57; 4; 11-2017; 331-337
1899-007X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/https://content.sciendo.com/view/journals/jppr/ahead-of-print/article-10.1515-jppr-2017-0045.xml
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Polish Academy of Sciences
publisher.none.fl_str_mv Polish Academy of Sciences
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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