Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration

Autores
Bravo Ferrada, Barbara Mercedes; Gonçalves, S.; Semorile, Liliana Carmen; Santos, N.C.; Tymczyszyn, Emma Elizabeth; Hollmann, Axel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: In this work, we evaluated freeze-drying damage at the surface level of oenological strain Lactobacillus plantarum UNQLp155, as well as its ability to grow in a synthetic wine with and without pre-acclimation. Methods and Results: Damage on cell surface was studied by flow cytometry, zeta potential and atomic force microscopy, and cell survival was analysed by plate count. Results showed that beside cells acclimated at lower ethanol concentration (6% v/v) became more susceptible to drying than nonacclimated ones, after rehydration they maintain their increased ability to grow in a synthetic wine. Acclimation at a higher ethanol concentration (10% v/v) produces several damages on the cell surface losing its ability to grow in a synthetic wine. Conclusions: In this work, we showed for the first time that sublethal alterations on bacterial surface induced by a pre-acclimation with a low ethanol concentration (6%), upon a freeze-drying process, result in a better bacterial adaptation to the stress conditions of wine-like medium, as well as to the preservation process. Significance and Impact of the Study: Understanding the adaptation to ethanol of oenological strains and their effects on the preservation process has a strong impact on winemaking process and allows to define the most appropriate conditions to obtain malolactic starters cultures.
Fil: Bravo Ferrada, Barbara Mercedes. Universidad Nacional de Quilmes; Argentina
Fil: Gonçalves, S.. Universidades de Lisboa; Portugal
Fil: Semorile, Liliana Carmen. Universidad Nacional de Quilmes; Argentina
Fil: Santos, N.C.. Universidades de Lisboa; Portugal
Fil: Tymczyszyn, Emma Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Hollmann, Axel. Universidades de Lisboa; Portugal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina. Universidad Nacional de Quilmes; Argentina
Materia
Lactobacillus Plantarum
Atomic Force Microscopy
Freeze-Drying
Wine
Zeta Potential
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38434
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/38434
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydrationBravo Ferrada, Barbara MercedesGonçalves, S.Semorile, Liliana CarmenSantos, N.C.Tymczyszyn, Emma ElizabethHollmann, AxelLactobacillus PlantarumAtomic Force MicroscopyFreeze-DryingWineZeta Potentialhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aims: In this work, we evaluated freeze-drying damage at the surface level of oenological strain Lactobacillus plantarum UNQLp155, as well as its ability to grow in a synthetic wine with and without pre-acclimation. Methods and Results: Damage on cell surface was studied by flow cytometry, zeta potential and atomic force microscopy, and cell survival was analysed by plate count. Results showed that beside cells acclimated at lower ethanol concentration (6% v/v) became more susceptible to drying than nonacclimated ones, after rehydration they maintain their increased ability to grow in a synthetic wine. Acclimation at a higher ethanol concentration (10% v/v) produces several damages on the cell surface losing its ability to grow in a synthetic wine. Conclusions: In this work, we showed for the first time that sublethal alterations on bacterial surface induced by a pre-acclimation with a low ethanol concentration (6%), upon a freeze-drying process, result in a better bacterial adaptation to the stress conditions of wine-like medium, as well as to the preservation process. Significance and Impact of the Study: Understanding the adaptation to ethanol of oenological strains and their effects on the preservation process has a strong impact on winemaking process and allows to define the most appropriate conditions to obtain malolactic starters cultures.Fil: Bravo Ferrada, Barbara Mercedes. Universidad Nacional de Quilmes; ArgentinaFil: Gonçalves, S.. Universidades de Lisboa; PortugalFil: Semorile, Liliana Carmen. Universidad Nacional de Quilmes; ArgentinaFil: Santos, N.C.. Universidades de Lisboa; PortugalFil: Tymczyszyn, Emma Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; ArgentinaFil: Hollmann, Axel. Universidades de Lisboa; Portugal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina. Universidad Nacional de Quilmes; ArgentinaWiley Blackwell Publishing, Inc2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38434Bravo Ferrada, Barbara Mercedes; Gonçalves, S.; Semorile, Liliana Carmen; Santos, N.C.; Tymczyszyn, Emma Elizabeth; et al.; Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 118; 6; 6-2015; 1409-14171364-5072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jam.12796/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/jam.12796info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:18:16Zoai:ri.conicet.gov.ar:11336/38434instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:18:17.299CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
title Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
spellingShingle Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
Bravo Ferrada, Barbara Mercedes
Lactobacillus Plantarum
Atomic Force Microscopy
Freeze-Drying
Wine
Zeta Potential
title_short Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
title_full Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
title_fullStr Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
title_full_unstemmed Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
title_sort Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
dc.creator.none.fl_str_mv Bravo Ferrada, Barbara Mercedes
Gonçalves, S.
Semorile, Liliana Carmen
Santos, N.C.
Tymczyszyn, Emma Elizabeth
Hollmann, Axel
author Bravo Ferrada, Barbara Mercedes
author_facet Bravo Ferrada, Barbara Mercedes
Gonçalves, S.
Semorile, Liliana Carmen
Santos, N.C.
Tymczyszyn, Emma Elizabeth
Hollmann, Axel
author_role author
author2 Gonçalves, S.
Semorile, Liliana Carmen
Santos, N.C.
Tymczyszyn, Emma Elizabeth
Hollmann, Axel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Lactobacillus Plantarum
Atomic Force Microscopy
Freeze-Drying
Wine
Zeta Potential
topic Lactobacillus Plantarum
Atomic Force Microscopy
Freeze-Drying
Wine
Zeta Potential
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aims: In this work, we evaluated freeze-drying damage at the surface level of oenological strain Lactobacillus plantarum UNQLp155, as well as its ability to grow in a synthetic wine with and without pre-acclimation. Methods and Results: Damage on cell surface was studied by flow cytometry, zeta potential and atomic force microscopy, and cell survival was analysed by plate count. Results showed that beside cells acclimated at lower ethanol concentration (6% v/v) became more susceptible to drying than nonacclimated ones, after rehydration they maintain their increased ability to grow in a synthetic wine. Acclimation at a higher ethanol concentration (10% v/v) produces several damages on the cell surface losing its ability to grow in a synthetic wine. Conclusions: In this work, we showed for the first time that sublethal alterations on bacterial surface induced by a pre-acclimation with a low ethanol concentration (6%), upon a freeze-drying process, result in a better bacterial adaptation to the stress conditions of wine-like medium, as well as to the preservation process. Significance and Impact of the Study: Understanding the adaptation to ethanol of oenological strains and their effects on the preservation process has a strong impact on winemaking process and allows to define the most appropriate conditions to obtain malolactic starters cultures.
Fil: Bravo Ferrada, Barbara Mercedes. Universidad Nacional de Quilmes; Argentina
Fil: Gonçalves, S.. Universidades de Lisboa; Portugal
Fil: Semorile, Liliana Carmen. Universidad Nacional de Quilmes; Argentina
Fil: Santos, N.C.. Universidades de Lisboa; Portugal
Fil: Tymczyszyn, Emma Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Hollmann, Axel. Universidades de Lisboa; Portugal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina. Universidad Nacional de Quilmes; Argentina
description Aims: In this work, we evaluated freeze-drying damage at the surface level of oenological strain Lactobacillus plantarum UNQLp155, as well as its ability to grow in a synthetic wine with and without pre-acclimation. Methods and Results: Damage on cell surface was studied by flow cytometry, zeta potential and atomic force microscopy, and cell survival was analysed by plate count. Results showed that beside cells acclimated at lower ethanol concentration (6% v/v) became more susceptible to drying than nonacclimated ones, after rehydration they maintain their increased ability to grow in a synthetic wine. Acclimation at a higher ethanol concentration (10% v/v) produces several damages on the cell surface losing its ability to grow in a synthetic wine. Conclusions: In this work, we showed for the first time that sublethal alterations on bacterial surface induced by a pre-acclimation with a low ethanol concentration (6%), upon a freeze-drying process, result in a better bacterial adaptation to the stress conditions of wine-like medium, as well as to the preservation process. Significance and Impact of the Study: Understanding the adaptation to ethanol of oenological strains and their effects on the preservation process has a strong impact on winemaking process and allows to define the most appropriate conditions to obtain malolactic starters cultures.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38434
Bravo Ferrada, Barbara Mercedes; Gonçalves, S.; Semorile, Liliana Carmen; Santos, N.C.; Tymczyszyn, Emma Elizabeth; et al.; Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 118; 6; 6-2015; 1409-1417
1364-5072
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38434
identifier_str_mv Bravo Ferrada, Barbara Mercedes; Gonçalves, S.; Semorile, Liliana Carmen; Santos, N.C.; Tymczyszyn, Emma Elizabeth; et al.; Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 118; 6; 6-2015; 1409-1417
1364-5072
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jam.12796/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/jam.12796
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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