Biopolymer nanoparticles designed for polyunsaturated fatty acid vehiculization: Protein-polysaccharide ratio study

Autores
Perez, Adrián Alejandro; Sponton, Osvaldo Ernesto; Andermatten, Romina Belén; Rubiolo, Amelia Catalina; Santiago, Liliana Gabriela
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Information about the design of biopolymer nanoparticles (BNPs) for polyunsaturated fatty acid (PUFA) vehiculization is provided. Linoleic acid (LA) was used as a model PUFA. The binding ability of LA to β-lactoglobulin (BLG) was applied for obtaining BLG-LA complexes. BLG-LA complex formation was monitored by fluorimetry and it was observed that a moderate heat treatment (60 °C, 10 min) enhanced BLG-LA complexation. Obtaining BNPs involved the electrostatic deposition of high methoxyl pectin (HMP) onto the BLG-LA complex surface. The phase behavior of biopolymer systems was discussed at different Prot:HMP ratio (RProt:HMP, wt.%) levels (1:1-6:1). Absorbance at 600 nm, particle size, and ζ potential were analyzed at pH 4.0. At 1:1-2:1 RProt:HMP, BNPs showed appreciable turbidity, a nanometric diameter (337-364 nm), and a negative ζ potential. Finally, intrinsic and extrinsic fluorimetry was used for examining the HMP protective role at the LA binding site. At 2:1 RProt:HMP, HMP cover could promote significant LA protection in BNPs.
Fil: Perez, Adrián Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Fil: Sponton, Osvaldo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Fil: Andermatten, Romina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Fil: Rubiolo, Amelia Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Fil: Santiago, Liliana Gabriela. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Materia
Binding Properties
Biopolymer Nanoparticles
High Methoxyl Pectin
Linoleic Acid
Vehiculization
Β-Lactoglobulin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50925
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/50925
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biopolymer nanoparticles designed for polyunsaturated fatty acid vehiculization: Protein-polysaccharide ratio studyPerez, Adrián AlejandroSponton, Osvaldo ErnestoAndermatten, Romina BelénRubiolo, Amelia CatalinaSantiago, Liliana GabrielaBinding PropertiesBiopolymer NanoparticlesHigh Methoxyl PectinLinoleic AcidVehiculizationΒ-Lactoglobulinhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Information about the design of biopolymer nanoparticles (BNPs) for polyunsaturated fatty acid (PUFA) vehiculization is provided. Linoleic acid (LA) was used as a model PUFA. The binding ability of LA to β-lactoglobulin (BLG) was applied for obtaining BLG-LA complexes. BLG-LA complex formation was monitored by fluorimetry and it was observed that a moderate heat treatment (60 °C, 10 min) enhanced BLG-LA complexation. Obtaining BNPs involved the electrostatic deposition of high methoxyl pectin (HMP) onto the BLG-LA complex surface. The phase behavior of biopolymer systems was discussed at different Prot:HMP ratio (RProt:HMP, wt.%) levels (1:1-6:1). Absorbance at 600 nm, particle size, and ζ potential were analyzed at pH 4.0. At 1:1-2:1 RProt:HMP, BNPs showed appreciable turbidity, a nanometric diameter (337-364 nm), and a negative ζ potential. Finally, intrinsic and extrinsic fluorimetry was used for examining the HMP protective role at the LA binding site. At 2:1 RProt:HMP, HMP cover could promote significant LA protection in BNPs.Fil: Perez, Adrián Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; ArgentinaFil: Sponton, Osvaldo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; ArgentinaFil: Andermatten, Romina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; ArgentinaFil: Rubiolo, Amelia Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; ArgentinaFil: Santiago, Liliana Gabriela. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; ArgentinaElsevier2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50925Perez, Adrián Alejandro; Sponton, Osvaldo Ernesto; Andermatten, Romina Belén; Rubiolo, Amelia Catalina; Santiago, Liliana Gabriela; Biopolymer nanoparticles designed for polyunsaturated fatty acid vehiculization: Protein-polysaccharide ratio study; Elsevier; Food Chemistry; 188; 5-2015; 543-5500308-8146CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2015.05.043info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0308814615007773info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:17:10Zoai:ri.conicet.gov.ar:11336/50925instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:17:10.81CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biopolymer nanoparticles designed for polyunsaturated fatty acid vehiculization: Protein-polysaccharide ratio study
title Biopolymer nanoparticles designed for polyunsaturated fatty acid vehiculization: Protein-polysaccharide ratio study
spellingShingle Biopolymer nanoparticles designed for polyunsaturated fatty acid vehiculization: Protein-polysaccharide ratio study
Perez, Adrián Alejandro
Binding Properties
Biopolymer Nanoparticles
High Methoxyl Pectin
Linoleic Acid
Vehiculization
Β-Lactoglobulin
title_short Biopolymer nanoparticles designed for polyunsaturated fatty acid vehiculization: Protein-polysaccharide ratio study
title_full Biopolymer nanoparticles designed for polyunsaturated fatty acid vehiculization: Protein-polysaccharide ratio study
title_fullStr Biopolymer nanoparticles designed for polyunsaturated fatty acid vehiculization: Protein-polysaccharide ratio study
title_full_unstemmed Biopolymer nanoparticles designed for polyunsaturated fatty acid vehiculization: Protein-polysaccharide ratio study
title_sort Biopolymer nanoparticles designed for polyunsaturated fatty acid vehiculization: Protein-polysaccharide ratio study
dc.creator.none.fl_str_mv Perez, Adrián Alejandro
Sponton, Osvaldo Ernesto
Andermatten, Romina Belén
Rubiolo, Amelia Catalina
Santiago, Liliana Gabriela
author Perez, Adrián Alejandro
author_facet Perez, Adrián Alejandro
Sponton, Osvaldo Ernesto
Andermatten, Romina Belén
Rubiolo, Amelia Catalina
Santiago, Liliana Gabriela
author_role author
author2 Sponton, Osvaldo Ernesto
Andermatten, Romina Belén
Rubiolo, Amelia Catalina
Santiago, Liliana Gabriela
author2_role author
author
author
author
dc.subject.none.fl_str_mv Binding Properties
Biopolymer Nanoparticles
High Methoxyl Pectin
Linoleic Acid
Vehiculization
Β-Lactoglobulin
topic Binding Properties
Biopolymer Nanoparticles
High Methoxyl Pectin
Linoleic Acid
Vehiculization
Β-Lactoglobulin
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Information about the design of biopolymer nanoparticles (BNPs) for polyunsaturated fatty acid (PUFA) vehiculization is provided. Linoleic acid (LA) was used as a model PUFA. The binding ability of LA to β-lactoglobulin (BLG) was applied for obtaining BLG-LA complexes. BLG-LA complex formation was monitored by fluorimetry and it was observed that a moderate heat treatment (60 °C, 10 min) enhanced BLG-LA complexation. Obtaining BNPs involved the electrostatic deposition of high methoxyl pectin (HMP) onto the BLG-LA complex surface. The phase behavior of biopolymer systems was discussed at different Prot:HMP ratio (RProt:HMP, wt.%) levels (1:1-6:1). Absorbance at 600 nm, particle size, and ζ potential were analyzed at pH 4.0. At 1:1-2:1 RProt:HMP, BNPs showed appreciable turbidity, a nanometric diameter (337-364 nm), and a negative ζ potential. Finally, intrinsic and extrinsic fluorimetry was used for examining the HMP protective role at the LA binding site. At 2:1 RProt:HMP, HMP cover could promote significant LA protection in BNPs.
Fil: Perez, Adrián Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Fil: Sponton, Osvaldo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Fil: Andermatten, Romina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Fil: Rubiolo, Amelia Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Fil: Santiago, Liliana Gabriela. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
description Information about the design of biopolymer nanoparticles (BNPs) for polyunsaturated fatty acid (PUFA) vehiculization is provided. Linoleic acid (LA) was used as a model PUFA. The binding ability of LA to β-lactoglobulin (BLG) was applied for obtaining BLG-LA complexes. BLG-LA complex formation was monitored by fluorimetry and it was observed that a moderate heat treatment (60 °C, 10 min) enhanced BLG-LA complexation. Obtaining BNPs involved the electrostatic deposition of high methoxyl pectin (HMP) onto the BLG-LA complex surface. The phase behavior of biopolymer systems was discussed at different Prot:HMP ratio (RProt:HMP, wt.%) levels (1:1-6:1). Absorbance at 600 nm, particle size, and ζ potential were analyzed at pH 4.0. At 1:1-2:1 RProt:HMP, BNPs showed appreciable turbidity, a nanometric diameter (337-364 nm), and a negative ζ potential. Finally, intrinsic and extrinsic fluorimetry was used for examining the HMP protective role at the LA binding site. At 2:1 RProt:HMP, HMP cover could promote significant LA protection in BNPs.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50925
Perez, Adrián Alejandro; Sponton, Osvaldo Ernesto; Andermatten, Romina Belén; Rubiolo, Amelia Catalina; Santiago, Liliana Gabriela; Biopolymer nanoparticles designed for polyunsaturated fatty acid vehiculization: Protein-polysaccharide ratio study; Elsevier; Food Chemistry; 188; 5-2015; 543-550
0308-8146
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50925
identifier_str_mv Perez, Adrián Alejandro; Sponton, Osvaldo Ernesto; Andermatten, Romina Belén; Rubiolo, Amelia Catalina; Santiago, Liliana Gabriela; Biopolymer nanoparticles designed for polyunsaturated fatty acid vehiculization: Protein-polysaccharide ratio study; Elsevier; Food Chemistry; 188; 5-2015; 543-550
0308-8146
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2015.05.043
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0308814615007773
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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