The Drosophila Neuropeptides PDF and sNPF Have Opposing Electrophysiological and Molecular Effects on Central Neurons

Autores
Pírez, Nicolas; Vecsey, Christopher G.; Griffith, Leslie C.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neuropeptides have widespread effects on behavior, but how these molecules alter the activity of their target cells is poorly understood. We employed a new model system in Drosophila melanogaster to assess the electrophysiological and molecular effects of neuropeptides, recording in situ from larval motor neurons, which transgenically express a receptor of choice. We focused on two neuropeptides, pigment-dispersing factor (PDF) and small neuropeptide F (sNPF), which play important roles in sleep/rhythms and feeding/metabolism. PDF treatment depolarized motor neurons expressing the PDF receptor (PDFR), increasing excitability. sNPF treatment had the opposite effect, hyperpolarizing neurons expressing the sNPF receptor (sNPFR). Live optical imaging using a genetically encoded fluorescence resonance energy transfer (FRET)-based sensor for cyclic AMP (cAMP) showed that PDF induced a large increase in cAMP, whereas sNPF caused a small but significant decrease in cAMP. Coexpression of pertussis toxin or RNAi interference to disrupt the G-protein Gαo blocked the electrophysiological responses to sNPF, showing that sNPFR acts via Gαo signaling. Using a fluorescent sensor for intracellular calcium, we observed that sNPF-induced hyperpolarization blocked spontaneous waves of activity propagating along the ventral nerve cord, demonstrating that the electrical effects of sNPF can cause profound changes in natural network activity in the brain. This new model system provides a platform for mechanistic analysis of how neuropeptides can affect target cells at the electrical and molecular level, allowing for predictions of how they regulate brain circuits that control behaviors such as sleep and feeding.
Fil: Pírez, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Brandeis University. Department of Biology; Estados Unidos
Fil: Vecsey, Christopher G.. Brandeis University. Department of Biology; Estados Unidos
Fil: Griffith, Leslie C.. Brandeis University. Department of Biology; Estados Unidos
Materia
Camp
Pigment Dispersing Factor
Sleep
Small Neuropeptide F
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8339
Acceder
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network_name_str CONICET Digital (CONICET)
spelling The Drosophila Neuropeptides PDF and sNPF Have Opposing Electrophysiological and Molecular Effects on Central NeuronsPírez, NicolasVecsey, Christopher G.Griffith, Leslie C.CampPigment Dispersing FactorSleepSmall Neuropeptide Fhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Neuropeptides have widespread effects on behavior, but how these molecules alter the activity of their target cells is poorly understood. We employed a new model system in Drosophila melanogaster to assess the electrophysiological and molecular effects of neuropeptides, recording in situ from larval motor neurons, which transgenically express a receptor of choice. We focused on two neuropeptides, pigment-dispersing factor (PDF) and small neuropeptide F (sNPF), which play important roles in sleep/rhythms and feeding/metabolism. PDF treatment depolarized motor neurons expressing the PDF receptor (PDFR), increasing excitability. sNPF treatment had the opposite effect, hyperpolarizing neurons expressing the sNPF receptor (sNPFR). Live optical imaging using a genetically encoded fluorescence resonance energy transfer (FRET)-based sensor for cyclic AMP (cAMP) showed that PDF induced a large increase in cAMP, whereas sNPF caused a small but significant decrease in cAMP. Coexpression of pertussis toxin or RNAi interference to disrupt the G-protein Gαo blocked the electrophysiological responses to sNPF, showing that sNPFR acts via Gαo signaling. Using a fluorescent sensor for intracellular calcium, we observed that sNPF-induced hyperpolarization blocked spontaneous waves of activity propagating along the ventral nerve cord, demonstrating that the electrical effects of sNPF can cause profound changes in natural network activity in the brain. This new model system provides a platform for mechanistic analysis of how neuropeptides can affect target cells at the electrical and molecular level, allowing for predictions of how they regulate brain circuits that control behaviors such as sleep and feeding.Fil: Pírez, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Brandeis University. Department of Biology; Estados UnidosFil: Vecsey, Christopher G.. Brandeis University. Department of Biology; Estados UnidosFil: Griffith, Leslie C.. Brandeis University. Department of Biology; Estados UnidosAmerican Physiological Society2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8339Pírez, Nicolas; Vecsey, Christopher G.; Griffith, Leslie C.; The Drosophila Neuropeptides PDF and sNPF Have Opposing Electrophysiological and Molecular Effects on Central Neurons; American Physiological Society; Journal Of Neurophysiology; 111; 5; 3-2014; 1033-10450022-3077enginfo:eu-repo/semantics/altIdentifier/url/http://jn.physiology.org/content/111/5/1033.article-infoinfo:eu-repo/semantics/altIdentifier/doi/10.1152/jn.00712.2013info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3949227/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:30Zoai:ri.conicet.gov.ar:11336/8339instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:31.2CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Drosophila Neuropeptides PDF and sNPF Have Opposing Electrophysiological and Molecular Effects on Central Neurons
title The Drosophila Neuropeptides PDF and sNPF Have Opposing Electrophysiological and Molecular Effects on Central Neurons
spellingShingle The Drosophila Neuropeptides PDF and sNPF Have Opposing Electrophysiological and Molecular Effects on Central Neurons
Pírez, Nicolas
Camp
Pigment Dispersing Factor
Sleep
Small Neuropeptide F
title_short The Drosophila Neuropeptides PDF and sNPF Have Opposing Electrophysiological and Molecular Effects on Central Neurons
title_full The Drosophila Neuropeptides PDF and sNPF Have Opposing Electrophysiological and Molecular Effects on Central Neurons
title_fullStr The Drosophila Neuropeptides PDF and sNPF Have Opposing Electrophysiological and Molecular Effects on Central Neurons
title_full_unstemmed The Drosophila Neuropeptides PDF and sNPF Have Opposing Electrophysiological and Molecular Effects on Central Neurons
title_sort The Drosophila Neuropeptides PDF and sNPF Have Opposing Electrophysiological and Molecular Effects on Central Neurons
dc.creator.none.fl_str_mv Pírez, Nicolas
Vecsey, Christopher G.
Griffith, Leslie C.
author Pírez, Nicolas
author_facet Pírez, Nicolas
Vecsey, Christopher G.
Griffith, Leslie C.
author_role author
author2 Vecsey, Christopher G.
Griffith, Leslie C.
author2_role author
author
dc.subject.none.fl_str_mv Camp
Pigment Dispersing Factor
Sleep
Small Neuropeptide F
topic Camp
Pigment Dispersing Factor
Sleep
Small Neuropeptide F
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Neuropeptides have widespread effects on behavior, but how these molecules alter the activity of their target cells is poorly understood. We employed a new model system in Drosophila melanogaster to assess the electrophysiological and molecular effects of neuropeptides, recording in situ from larval motor neurons, which transgenically express a receptor of choice. We focused on two neuropeptides, pigment-dispersing factor (PDF) and small neuropeptide F (sNPF), which play important roles in sleep/rhythms and feeding/metabolism. PDF treatment depolarized motor neurons expressing the PDF receptor (PDFR), increasing excitability. sNPF treatment had the opposite effect, hyperpolarizing neurons expressing the sNPF receptor (sNPFR). Live optical imaging using a genetically encoded fluorescence resonance energy transfer (FRET)-based sensor for cyclic AMP (cAMP) showed that PDF induced a large increase in cAMP, whereas sNPF caused a small but significant decrease in cAMP. Coexpression of pertussis toxin or RNAi interference to disrupt the G-protein Gαo blocked the electrophysiological responses to sNPF, showing that sNPFR acts via Gαo signaling. Using a fluorescent sensor for intracellular calcium, we observed that sNPF-induced hyperpolarization blocked spontaneous waves of activity propagating along the ventral nerve cord, demonstrating that the electrical effects of sNPF can cause profound changes in natural network activity in the brain. This new model system provides a platform for mechanistic analysis of how neuropeptides can affect target cells at the electrical and molecular level, allowing for predictions of how they regulate brain circuits that control behaviors such as sleep and feeding.
Fil: Pírez, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Brandeis University. Department of Biology; Estados Unidos
Fil: Vecsey, Christopher G.. Brandeis University. Department of Biology; Estados Unidos
Fil: Griffith, Leslie C.. Brandeis University. Department of Biology; Estados Unidos
description Neuropeptides have widespread effects on behavior, but how these molecules alter the activity of their target cells is poorly understood. We employed a new model system in Drosophila melanogaster to assess the electrophysiological and molecular effects of neuropeptides, recording in situ from larval motor neurons, which transgenically express a receptor of choice. We focused on two neuropeptides, pigment-dispersing factor (PDF) and small neuropeptide F (sNPF), which play important roles in sleep/rhythms and feeding/metabolism. PDF treatment depolarized motor neurons expressing the PDF receptor (PDFR), increasing excitability. sNPF treatment had the opposite effect, hyperpolarizing neurons expressing the sNPF receptor (sNPFR). Live optical imaging using a genetically encoded fluorescence resonance energy transfer (FRET)-based sensor for cyclic AMP (cAMP) showed that PDF induced a large increase in cAMP, whereas sNPF caused a small but significant decrease in cAMP. Coexpression of pertussis toxin or RNAi interference to disrupt the G-protein Gαo blocked the electrophysiological responses to sNPF, showing that sNPFR acts via Gαo signaling. Using a fluorescent sensor for intracellular calcium, we observed that sNPF-induced hyperpolarization blocked spontaneous waves of activity propagating along the ventral nerve cord, demonstrating that the electrical effects of sNPF can cause profound changes in natural network activity in the brain. This new model system provides a platform for mechanistic analysis of how neuropeptides can affect target cells at the electrical and molecular level, allowing for predictions of how they regulate brain circuits that control behaviors such as sleep and feeding.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8339
Pírez, Nicolas; Vecsey, Christopher G.; Griffith, Leslie C.; The Drosophila Neuropeptides PDF and sNPF Have Opposing Electrophysiological and Molecular Effects on Central Neurons; American Physiological Society; Journal Of Neurophysiology; 111; 5; 3-2014; 1033-1045
0022-3077
url http://hdl.handle.net/11336/8339
identifier_str_mv Pírez, Nicolas; Vecsey, Christopher G.; Griffith, Leslie C.; The Drosophila Neuropeptides PDF and sNPF Have Opposing Electrophysiological and Molecular Effects on Central Neurons; American Physiological Society; Journal Of Neurophysiology; 111; 5; 3-2014; 1033-1045
0022-3077
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jn.physiology.org/content/111/5/1033.article-info
info:eu-repo/semantics/altIdentifier/doi/10.1152/jn.00712.2013
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3949227/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Physiological Society
publisher.none.fl_str_mv American Physiological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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