Partition in aqueous two-phase system: its application in downstream processing of tannase from Aspergillus niger

Autores
Rodríguez Durán, Luis; Spelzini, Darío; Boeris, Valeria; Cristobal Aguilar, N.; Picó, Guillermo Alfredo
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tannase from Aspergillus niger was partitioned in aqueous two-phase systems composed by polyethyleneglycol of molar mass 400, 600 and 1000 and potassium phosphate. Tannase was found to be partitioned toward the salt-rich phase in all systems, with partition coefficients lower than 0.5. Partition coefficients values and low entropic and enthalpic changes associated with tannase partition suggest that the entropic effect may be the driving force of the concentration of the enzyme in the bottom phase due to the high molar mass of the enzyme. The process was significantly influenced by the top phase/bottom phase volume ratio. When the fungal culture broth was partitioned in these systems, a good performance was found, since the enzyme recovery in the bottom phase of the system composed by polyethyleneglycol 1000 was around 96% with a 7.0-fold increase in purity.
Fil: Rodríguez Durán, Luis. Universidad Autónoma de Coahuila; México
Fil: Spelzini, Darío. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Fil: Boeris, Valeria. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Fil: Cristobal Aguilar, N.. Universidad Autónoma de Coahuila; México
Fil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Materia
Aqueous Two Phase System
Tannase
Partition
Purification
Interaction
Downstream Processing
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7469
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Partition in aqueous two-phase system: its application in downstream processing of tannase from Aspergillus nigerRodríguez Durán, LuisSpelzini, DaríoBoeris, ValeriaCristobal Aguilar, N.Picó, Guillermo AlfredoAqueous Two Phase SystemTannasePartitionPurificationInteractionDownstream Processinghttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Tannase from Aspergillus niger was partitioned in aqueous two-phase systems composed by polyethyleneglycol of molar mass 400, 600 and 1000 and potassium phosphate. Tannase was found to be partitioned toward the salt-rich phase in all systems, with partition coefficients lower than 0.5. Partition coefficients values and low entropic and enthalpic changes associated with tannase partition suggest that the entropic effect may be the driving force of the concentration of the enzyme in the bottom phase due to the high molar mass of the enzyme. The process was significantly influenced by the top phase/bottom phase volume ratio. When the fungal culture broth was partitioned in these systems, a good performance was found, since the enzyme recovery in the bottom phase of the system composed by polyethyleneglycol 1000 was around 96% with a 7.0-fold increase in purity.Fil: Rodríguez Durán, Luis. Universidad Autónoma de Coahuila; MéxicoFil: Spelzini, Darío. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; ArgentinaFil: Boeris, Valeria. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; ArgentinaFil: Cristobal Aguilar, N.. Universidad Autónoma de Coahuila; MéxicoFil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; ArgentinaElsevier2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7469Rodríguez Durán, Luis; Spelzini, Darío; Boeris, Valeria; Cristobal Aguilar, N.; Picó, Guillermo Alfredo; Partition in aqueous two-phase system: its application in downstream processing of tannase from Aspergillus niger; Elsevier; Colloids And Surfaces B: Biointerfaces; 101; 1-2013; 392-3970927-7765enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0927776512004171info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2012.07.023info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:11Zoai:ri.conicet.gov.ar:11336/7469instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:12.243CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Partition in aqueous two-phase system: its application in downstream processing of tannase from Aspergillus niger
title Partition in aqueous two-phase system: its application in downstream processing of tannase from Aspergillus niger
spellingShingle Partition in aqueous two-phase system: its application in downstream processing of tannase from Aspergillus niger
Rodríguez Durán, Luis
Aqueous Two Phase System
Tannase
Partition
Purification
Interaction
Downstream Processing
title_short Partition in aqueous two-phase system: its application in downstream processing of tannase from Aspergillus niger
title_full Partition in aqueous two-phase system: its application in downstream processing of tannase from Aspergillus niger
title_fullStr Partition in aqueous two-phase system: its application in downstream processing of tannase from Aspergillus niger
title_full_unstemmed Partition in aqueous two-phase system: its application in downstream processing of tannase from Aspergillus niger
title_sort Partition in aqueous two-phase system: its application in downstream processing of tannase from Aspergillus niger
dc.creator.none.fl_str_mv Rodríguez Durán, Luis
Spelzini, Darío
Boeris, Valeria
Cristobal Aguilar, N.
Picó, Guillermo Alfredo
author Rodríguez Durán, Luis
author_facet Rodríguez Durán, Luis
Spelzini, Darío
Boeris, Valeria
Cristobal Aguilar, N.
Picó, Guillermo Alfredo
author_role author
author2 Spelzini, Darío
Boeris, Valeria
Cristobal Aguilar, N.
Picó, Guillermo Alfredo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Aqueous Two Phase System
Tannase
Partition
Purification
Interaction
Downstream Processing
topic Aqueous Two Phase System
Tannase
Partition
Purification
Interaction
Downstream Processing
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Tannase from Aspergillus niger was partitioned in aqueous two-phase systems composed by polyethyleneglycol of molar mass 400, 600 and 1000 and potassium phosphate. Tannase was found to be partitioned toward the salt-rich phase in all systems, with partition coefficients lower than 0.5. Partition coefficients values and low entropic and enthalpic changes associated with tannase partition suggest that the entropic effect may be the driving force of the concentration of the enzyme in the bottom phase due to the high molar mass of the enzyme. The process was significantly influenced by the top phase/bottom phase volume ratio. When the fungal culture broth was partitioned in these systems, a good performance was found, since the enzyme recovery in the bottom phase of the system composed by polyethyleneglycol 1000 was around 96% with a 7.0-fold increase in purity.
Fil: Rodríguez Durán, Luis. Universidad Autónoma de Coahuila; México
Fil: Spelzini, Darío. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Fil: Boeris, Valeria. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
Fil: Cristobal Aguilar, N.. Universidad Autónoma de Coahuila; México
Fil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentina
description Tannase from Aspergillus niger was partitioned in aqueous two-phase systems composed by polyethyleneglycol of molar mass 400, 600 and 1000 and potassium phosphate. Tannase was found to be partitioned toward the salt-rich phase in all systems, with partition coefficients lower than 0.5. Partition coefficients values and low entropic and enthalpic changes associated with tannase partition suggest that the entropic effect may be the driving force of the concentration of the enzyme in the bottom phase due to the high molar mass of the enzyme. The process was significantly influenced by the top phase/bottom phase volume ratio. When the fungal culture broth was partitioned in these systems, a good performance was found, since the enzyme recovery in the bottom phase of the system composed by polyethyleneglycol 1000 was around 96% with a 7.0-fold increase in purity.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7469
Rodríguez Durán, Luis; Spelzini, Darío; Boeris, Valeria; Cristobal Aguilar, N.; Picó, Guillermo Alfredo; Partition in aqueous two-phase system: its application in downstream processing of tannase from Aspergillus niger; Elsevier; Colloids And Surfaces B: Biointerfaces; 101; 1-2013; 392-397
0927-7765
url http://hdl.handle.net/11336/7469
identifier_str_mv Rodríguez Durán, Luis; Spelzini, Darío; Boeris, Valeria; Cristobal Aguilar, N.; Picó, Guillermo Alfredo; Partition in aqueous two-phase system: its application in downstream processing of tannase from Aspergillus niger; Elsevier; Colloids And Surfaces B: Biointerfaces; 101; 1-2013; 392-397
0927-7765
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0927776512004171
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2012.07.023
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269743171502080
score 13.13397

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