Light fractionated ALA-PDT enhances therapeutic efficacy in vitro; The influence of PpIX concentration and illumination parameters

Autores
De Bruijn, Henriëtte S.; Casas, Adriana Gabriela; Di Venosa, Gabriela Mariana; Gándara, Lautaro; Sterenborg, Henricus J. C. M.; Batlle, Alcira María del C.; Robinson, Dominic J.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Light fractionation, with a long dark interval, significantly increases the response to ALA-PDT in pre-clinical models and in non-melanoma skin cancer. We investigated if this increase in efficacy can be replicated in PAM 212 cells in vitro. The results show a significant decrease in cell survival after light fractionation which is dependent on the PpIX concentration and light dose of the first light fraction. This study supports the hypothesis that an underlying cellular mechanism is involved in the response to light fractionation in which a first light fraction leads to sub-lethally damaged cells that are sensitised to a second light fraction 2 hours later. The current study reveals the in vitro circumstances under which we can investigate the cellular pathways involved.
Fil: De Bruijn, Henriëtte S.. Erasmus MC; Países Bajos
Fil: Casas, Adriana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Di Venosa, Gabriela Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Gándara, Lautaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Sterenborg, Henricus J. C. M.. Erasmus MC; Países Bajos
Fil: Batlle, Alcira María del C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Robinson, Dominic J.. Erasmus MC; Países Bajos
Materia
ALA
PHOTODYNAMIC THERAPY
FRACTIONATION
LIGHT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84738
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/84738
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Light fractionated ALA-PDT enhances therapeutic efficacy in vitro; The influence of PpIX concentration and illumination parametersDe Bruijn, Henriëtte S.Casas, Adriana GabrielaDi Venosa, Gabriela MarianaGándara, LautaroSterenborg, Henricus J. C. M.Batlle, Alcira María del C.Robinson, Dominic J.ALAPHOTODYNAMIC THERAPYFRACTIONATIONLIGHThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Light fractionation, with a long dark interval, significantly increases the response to ALA-PDT in pre-clinical models and in non-melanoma skin cancer. We investigated if this increase in efficacy can be replicated in PAM 212 cells in vitro. The results show a significant decrease in cell survival after light fractionation which is dependent on the PpIX concentration and light dose of the first light fraction. This study supports the hypothesis that an underlying cellular mechanism is involved in the response to light fractionation in which a first light fraction leads to sub-lethally damaged cells that are sensitised to a second light fraction 2 hours later. The current study reveals the in vitro circumstances under which we can investigate the cellular pathways involved.Fil: De Bruijn, Henriëtte S.. Erasmus MC; Países BajosFil: Casas, Adriana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; ArgentinaFil: Di Venosa, Gabriela Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; ArgentinaFil: Gándara, Lautaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; ArgentinaFil: Sterenborg, Henricus J. C. M.. Erasmus MC; Países BajosFil: Batlle, Alcira María del C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; ArgentinaFil: Robinson, Dominic J.. Erasmus MC; Países BajosRoyal Society of Chemistry2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84738De Bruijn, Henriëtte S.; Casas, Adriana Gabriela; Di Venosa, Gabriela Mariana; Gándara, Lautaro; Sterenborg, Henricus J. C. M.; et al.; Light fractionated ALA-PDT enhances therapeutic efficacy in vitro; The influence of PpIX concentration and illumination parameters; Royal Society of Chemistry; Photochemical and Photobiological Sciences; 12; 2; 2-2013; 241-2451474-905XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1039/c2pp25287binfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:17Zoai:ri.conicet.gov.ar:11336/84738instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:17.497CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Light fractionated ALA-PDT enhances therapeutic efficacy in vitro; The influence of PpIX concentration and illumination parameters
title Light fractionated ALA-PDT enhances therapeutic efficacy in vitro; The influence of PpIX concentration and illumination parameters
spellingShingle Light fractionated ALA-PDT enhances therapeutic efficacy in vitro; The influence of PpIX concentration and illumination parameters
De Bruijn, Henriëtte S.
ALA
PHOTODYNAMIC THERAPY
FRACTIONATION
LIGHT
title_short Light fractionated ALA-PDT enhances therapeutic efficacy in vitro; The influence of PpIX concentration and illumination parameters
title_full Light fractionated ALA-PDT enhances therapeutic efficacy in vitro; The influence of PpIX concentration and illumination parameters
title_fullStr Light fractionated ALA-PDT enhances therapeutic efficacy in vitro; The influence of PpIX concentration and illumination parameters
title_full_unstemmed Light fractionated ALA-PDT enhances therapeutic efficacy in vitro; The influence of PpIX concentration and illumination parameters
title_sort Light fractionated ALA-PDT enhances therapeutic efficacy in vitro; The influence of PpIX concentration and illumination parameters
dc.creator.none.fl_str_mv De Bruijn, Henriëtte S.
Casas, Adriana Gabriela
Di Venosa, Gabriela Mariana
Gándara, Lautaro
Sterenborg, Henricus J. C. M.
Batlle, Alcira María del C.
Robinson, Dominic J.
author De Bruijn, Henriëtte S.
author_facet De Bruijn, Henriëtte S.
Casas, Adriana Gabriela
Di Venosa, Gabriela Mariana
Gándara, Lautaro
Sterenborg, Henricus J. C. M.
Batlle, Alcira María del C.
Robinson, Dominic J.
author_role author
author2 Casas, Adriana Gabriela
Di Venosa, Gabriela Mariana
Gándara, Lautaro
Sterenborg, Henricus J. C. M.
Batlle, Alcira María del C.
Robinson, Dominic J.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ALA
PHOTODYNAMIC THERAPY
FRACTIONATION
LIGHT
topic ALA
PHOTODYNAMIC THERAPY
FRACTIONATION
LIGHT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Light fractionation, with a long dark interval, significantly increases the response to ALA-PDT in pre-clinical models and in non-melanoma skin cancer. We investigated if this increase in efficacy can be replicated in PAM 212 cells in vitro. The results show a significant decrease in cell survival after light fractionation which is dependent on the PpIX concentration and light dose of the first light fraction. This study supports the hypothesis that an underlying cellular mechanism is involved in the response to light fractionation in which a first light fraction leads to sub-lethally damaged cells that are sensitised to a second light fraction 2 hours later. The current study reveals the in vitro circumstances under which we can investigate the cellular pathways involved.
Fil: De Bruijn, Henriëtte S.. Erasmus MC; Países Bajos
Fil: Casas, Adriana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Di Venosa, Gabriela Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Gándara, Lautaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Sterenborg, Henricus J. C. M.. Erasmus MC; Países Bajos
Fil: Batlle, Alcira María del C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Robinson, Dominic J.. Erasmus MC; Países Bajos
description Light fractionation, with a long dark interval, significantly increases the response to ALA-PDT in pre-clinical models and in non-melanoma skin cancer. We investigated if this increase in efficacy can be replicated in PAM 212 cells in vitro. The results show a significant decrease in cell survival after light fractionation which is dependent on the PpIX concentration and light dose of the first light fraction. This study supports the hypothesis that an underlying cellular mechanism is involved in the response to light fractionation in which a first light fraction leads to sub-lethally damaged cells that are sensitised to a second light fraction 2 hours later. The current study reveals the in vitro circumstances under which we can investigate the cellular pathways involved.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84738
De Bruijn, Henriëtte S.; Casas, Adriana Gabriela; Di Venosa, Gabriela Mariana; Gándara, Lautaro; Sterenborg, Henricus J. C. M.; et al.; Light fractionated ALA-PDT enhances therapeutic efficacy in vitro; The influence of PpIX concentration and illumination parameters; Royal Society of Chemistry; Photochemical and Photobiological Sciences; 12; 2; 2-2013; 241-245
1474-905X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84738
identifier_str_mv De Bruijn, Henriëtte S.; Casas, Adriana Gabriela; Di Venosa, Gabriela Mariana; Gándara, Lautaro; Sterenborg, Henricus J. C. M.; et al.; Light fractionated ALA-PDT enhances therapeutic efficacy in vitro; The influence of PpIX concentration and illumination parameters; Royal Society of Chemistry; Photochemical and Photobiological Sciences; 12; 2; 2-2013; 241-245
1474-905X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1039/c2pp25287b
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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