Solar radiation exposure of dihydrobiopterin and biopterin in aqueous solution

Autores
Ipiña Hernandez, Adriana; Castaño Espinal, Diana Carolina; Dantola, Maria Laura; Thomas, Andrés Héctor
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dihydrobiopterin (H2Bip) and biopterin (Bip) accumulate in the skin of patients suffering from vitiligo, whose lack of melanin causes a deficient protection against UV radiation. H2Bip and Bip undergo photooxidation upon UV irradiation and the former acts as a photosensitizer of biomolecules. The aim of this work is to study the photochemical reactions of these compounds triggered by sunlight under outdoor conditions. H2Bip and Bip in aqueous solutions were exposed to sunlight in La Plata city (34.90°S, 57.92°W) from Spring equinox to near Summer solstice and the spectral solar irradiance was recorded with a high-resolution spectrometer. The photochemical reactions were followed by HPLC and UV-Vis spectrophotometry. Upon sun exposure, excitation of H2Bip leads to the formation of dimers and to its oxidation to Bip, which, in turn, is photooxidized into 6-formylpterin (Fop). Further excitation induces the oxidation of Fop to 6-carboxypterin, which is much more photostable than Bip and Fop and then it is accumulated in the solution. Rates of reactant consumption were determined under different weather conditions and the corresponding quantum yields were also calculated. We have demonstrated that solar radiation causes significant oxidation of the pterin derivatives investigated within a few minutes, even in cloudy days. Finally, the biological implications of our results are discussed.
Fil: Ipiña Hernandez, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Castaño Espinal, Diana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Dantola, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Thomas, Andrés Héctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
Materia
Dihydrobiopterin
Biopterin
Solar-Exposure
Photooxidation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5151
Acceder
id CONICETDig_4b5bf5330190b8d6d2cb97c2ecbb8c86
oai_identifier_str oai:ri.conicet.gov.ar:11336/5151
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Solar radiation exposure of dihydrobiopterin and biopterin in aqueous solutionIpiña Hernandez, AdrianaCastaño Espinal, Diana CarolinaDantola, Maria LauraThomas, Andrés HéctorDihydrobiopterinBiopterinSolar-ExposurePhotooxidationhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Dihydrobiopterin (H2Bip) and biopterin (Bip) accumulate in the skin of patients suffering from vitiligo, whose lack of melanin causes a deficient protection against UV radiation. H2Bip and Bip undergo photooxidation upon UV irradiation and the former acts as a photosensitizer of biomolecules. The aim of this work is to study the photochemical reactions of these compounds triggered by sunlight under outdoor conditions. H2Bip and Bip in aqueous solutions were exposed to sunlight in La Plata city (34.90°S, 57.92°W) from Spring equinox to near Summer solstice and the spectral solar irradiance was recorded with a high-resolution spectrometer. The photochemical reactions were followed by HPLC and UV-Vis spectrophotometry. Upon sun exposure, excitation of H2Bip leads to the formation of dimers and to its oxidation to Bip, which, in turn, is photooxidized into 6-formylpterin (Fop). Further excitation induces the oxidation of Fop to 6-carboxypterin, which is much more photostable than Bip and Fop and then it is accumulated in the solution. Rates of reactant consumption were determined under different weather conditions and the corresponding quantum yields were also calculated. We have demonstrated that solar radiation causes significant oxidation of the pterin derivatives investigated within a few minutes, even in cloudy days. Finally, the biological implications of our results are discussed.Fil: Ipiña Hernandez, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; ArgentinaFil: Castaño Espinal, Diana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; ArgentinaFil: Dantola, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; ArgentinaFil: Thomas, Andrés Héctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; ArgentinaElsevier2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5151Ipiña Hernandez, Adriana; Castaño Espinal, Diana Carolina; Dantola, Maria Laura; Thomas, Andrés Héctor; Solar radiation exposure of dihydrobiopterin and biopterin in aqueous solution; Elsevier; Solar Energy; 109; 11-2014; 45-530038-092Xenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0038092X14004022info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.solener.2014.08.025info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:29:00Zoai:ri.conicet.gov.ar:11336/5151instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:29:00.967CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Solar radiation exposure of dihydrobiopterin and biopterin in aqueous solution
title Solar radiation exposure of dihydrobiopterin and biopterin in aqueous solution
spellingShingle Solar radiation exposure of dihydrobiopterin and biopterin in aqueous solution
Ipiña Hernandez, Adriana
Dihydrobiopterin
Biopterin
Solar-Exposure
Photooxidation
title_short Solar radiation exposure of dihydrobiopterin and biopterin in aqueous solution
title_full Solar radiation exposure of dihydrobiopterin and biopterin in aqueous solution
title_fullStr Solar radiation exposure of dihydrobiopterin and biopterin in aqueous solution
title_full_unstemmed Solar radiation exposure of dihydrobiopterin and biopterin in aqueous solution
title_sort Solar radiation exposure of dihydrobiopterin and biopterin in aqueous solution
dc.creator.none.fl_str_mv Ipiña Hernandez, Adriana
Castaño Espinal, Diana Carolina
Dantola, Maria Laura
Thomas, Andrés Héctor
author Ipiña Hernandez, Adriana
author_facet Ipiña Hernandez, Adriana
Castaño Espinal, Diana Carolina
Dantola, Maria Laura
Thomas, Andrés Héctor
author_role author
author2 Castaño Espinal, Diana Carolina
Dantola, Maria Laura
Thomas, Andrés Héctor
author2_role author
author
author
dc.subject.none.fl_str_mv Dihydrobiopterin
Biopterin
Solar-Exposure
Photooxidation
topic Dihydrobiopterin
Biopterin
Solar-Exposure
Photooxidation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Dihydrobiopterin (H2Bip) and biopterin (Bip) accumulate in the skin of patients suffering from vitiligo, whose lack of melanin causes a deficient protection against UV radiation. H2Bip and Bip undergo photooxidation upon UV irradiation and the former acts as a photosensitizer of biomolecules. The aim of this work is to study the photochemical reactions of these compounds triggered by sunlight under outdoor conditions. H2Bip and Bip in aqueous solutions were exposed to sunlight in La Plata city (34.90°S, 57.92°W) from Spring equinox to near Summer solstice and the spectral solar irradiance was recorded with a high-resolution spectrometer. The photochemical reactions were followed by HPLC and UV-Vis spectrophotometry. Upon sun exposure, excitation of H2Bip leads to the formation of dimers and to its oxidation to Bip, which, in turn, is photooxidized into 6-formylpterin (Fop). Further excitation induces the oxidation of Fop to 6-carboxypterin, which is much more photostable than Bip and Fop and then it is accumulated in the solution. Rates of reactant consumption were determined under different weather conditions and the corresponding quantum yields were also calculated. We have demonstrated that solar radiation causes significant oxidation of the pterin derivatives investigated within a few minutes, even in cloudy days. Finally, the biological implications of our results are discussed.
Fil: Ipiña Hernandez, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Castaño Espinal, Diana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Dantola, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Thomas, Andrés Héctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
description Dihydrobiopterin (H2Bip) and biopterin (Bip) accumulate in the skin of patients suffering from vitiligo, whose lack of melanin causes a deficient protection against UV radiation. H2Bip and Bip undergo photooxidation upon UV irradiation and the former acts as a photosensitizer of biomolecules. The aim of this work is to study the photochemical reactions of these compounds triggered by sunlight under outdoor conditions. H2Bip and Bip in aqueous solutions were exposed to sunlight in La Plata city (34.90°S, 57.92°W) from Spring equinox to near Summer solstice and the spectral solar irradiance was recorded with a high-resolution spectrometer. The photochemical reactions were followed by HPLC and UV-Vis spectrophotometry. Upon sun exposure, excitation of H2Bip leads to the formation of dimers and to its oxidation to Bip, which, in turn, is photooxidized into 6-formylpterin (Fop). Further excitation induces the oxidation of Fop to 6-carboxypterin, which is much more photostable than Bip and Fop and then it is accumulated in the solution. Rates of reactant consumption were determined under different weather conditions and the corresponding quantum yields were also calculated. We have demonstrated that solar radiation causes significant oxidation of the pterin derivatives investigated within a few minutes, even in cloudy days. Finally, the biological implications of our results are discussed.
publishDate 2014
dc.date.none.fl_str_mv 2014-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5151
Ipiña Hernandez, Adriana; Castaño Espinal, Diana Carolina; Dantola, Maria Laura; Thomas, Andrés Héctor; Solar radiation exposure of dihydrobiopterin and biopterin in aqueous solution; Elsevier; Solar Energy; 109; 11-2014; 45-53
0038-092X
url http://hdl.handle.net/11336/5151
identifier_str_mv Ipiña Hernandez, Adriana; Castaño Espinal, Diana Carolina; Dantola, Maria Laura; Thomas, Andrés Héctor; Solar radiation exposure of dihydrobiopterin and biopterin in aqueous solution; Elsevier; Solar Energy; 109; 11-2014; 45-53
0038-092X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0038092X14004022
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.solener.2014.08.025
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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