Marine bioprospecting: enzymes and stress proteins from the sea anemones Anthopleura dowii and Lebrunia neglecta

Autores
Ramírez Carreto, Santos; Miranda Zaragoza, Beatriz; Simões, Nuno; González Muñoz, Ricardo Enrique; Rodríguez Almazan, Claudia
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The bioprospecting of sea anemone tissues and secretions has revealed that they are naturallibraries of polypeptides with diverse biological activities that can be utilized to develop ofbiotechnological tools with potential medical and industrial applications. This study conducteda proteomic analysis of crude venom extracts from Anthopleura dowii Verrill, 1869, and Lebrunianeglecta Duchassaing & Michelotti, 1860. The obtained data allowed us to identify 201 polypeptides,of which 39% were present in both extracts. Among the obtained sequences, hydrolase-type enzymes,oxidoreductases, transferases, heat shock proteins, adhesion proteins, and protease inhibitors,among others, were identified. Interaction analysis and functional annotation indicated thatthese proteins are primarily involved in endoplasmic reticulum metabolic processes such ascarbon metabolism and protein processing. In addition, several proteins related to oxidative stresswere identified, including superoxide dismutase, peroxiredoxins, thioredoxin, and glutathione oxidase.Our results provide novel information on the polypeptide composition of the crude venomextract from sea anemones, which can be utilized to develop molecules for therapeutic tools andindustrial applications.
Fil: Ramírez Carreto, Santos. Instituto Nacional de Salud Pública; México
Fil: Miranda Zaragoza, Beatriz. Universidad Nacional Autónoma de México; México
Fil: Simões, Nuno. Universidad Nacional Autónoma de México; México. Texas A and M University-Corpus Christi; Estados Unidos
Fil: González Muñoz, Ricardo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Rodríguez Almazan, Claudia. Universidad Nacional Autónoma de México; México
Materia
Lebrunia neglecta
Antopleura dowii
proteome
crude venom extract
enzymes
sea anemone
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/234284
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/234284
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Marine bioprospecting: enzymes and stress proteins from the sea anemones Anthopleura dowii and Lebrunia neglectaRamírez Carreto, SantosMiranda Zaragoza, BeatrizSimões, NunoGonzález Muñoz, Ricardo EnriqueRodríguez Almazan, ClaudiaLebrunia neglectaAntopleura dowiiproteomecrude venom extractenzymessea anemonehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The bioprospecting of sea anemone tissues and secretions has revealed that they are naturallibraries of polypeptides with diverse biological activities that can be utilized to develop ofbiotechnological tools with potential medical and industrial applications. This study conducteda proteomic analysis of crude venom extracts from Anthopleura dowii Verrill, 1869, and Lebrunianeglecta Duchassaing & Michelotti, 1860. The obtained data allowed us to identify 201 polypeptides,of which 39% were present in both extracts. Among the obtained sequences, hydrolase-type enzymes,oxidoreductases, transferases, heat shock proteins, adhesion proteins, and protease inhibitors,among others, were identified. Interaction analysis and functional annotation indicated thatthese proteins are primarily involved in endoplasmic reticulum metabolic processes such ascarbon metabolism and protein processing. In addition, several proteins related to oxidative stresswere identified, including superoxide dismutase, peroxiredoxins, thioredoxin, and glutathione oxidase.Our results provide novel information on the polypeptide composition of the crude venomextract from sea anemones, which can be utilized to develop molecules for therapeutic tools andindustrial applications.Fil: Ramírez Carreto, Santos. Instituto Nacional de Salud Pública; MéxicoFil: Miranda Zaragoza, Beatriz. Universidad Nacional Autónoma de México; MéxicoFil: Simões, Nuno. Universidad Nacional Autónoma de México; México. Texas A and M University-Corpus Christi; Estados UnidosFil: González Muñoz, Ricardo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Rodríguez Almazan, Claudia. Universidad Nacional Autónoma de México; MéxicoMolecular Diversity Preservation International2023-12-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/234284Ramírez Carreto, Santos; Miranda Zaragoza, Beatriz; Simões, Nuno; González Muñoz, Ricardo Enrique; Rodríguez Almazan, Claudia; Marine bioprospecting: enzymes and stress proteins from the sea anemones Anthopleura dowii and Lebrunia neglecta; Molecular Diversity Preservation International; Marine Drugs; 22; 1; 23-12-2023; 1-251660-3397CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1660-3397/22/1/12info:eu-repo/semantics/altIdentifier/doi/10.3390/md22010012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:07:34Zoai:ri.conicet.gov.ar:11336/234284instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:07:34.414CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Marine bioprospecting: enzymes and stress proteins from the sea anemones Anthopleura dowii and Lebrunia neglecta
title Marine bioprospecting: enzymes and stress proteins from the sea anemones Anthopleura dowii and Lebrunia neglecta
spellingShingle Marine bioprospecting: enzymes and stress proteins from the sea anemones Anthopleura dowii and Lebrunia neglecta
Ramírez Carreto, Santos
Lebrunia neglecta
Antopleura dowii
proteome
crude venom extract
enzymes
sea anemone
title_short Marine bioprospecting: enzymes and stress proteins from the sea anemones Anthopleura dowii and Lebrunia neglecta
title_full Marine bioprospecting: enzymes and stress proteins from the sea anemones Anthopleura dowii and Lebrunia neglecta
title_fullStr Marine bioprospecting: enzymes and stress proteins from the sea anemones Anthopleura dowii and Lebrunia neglecta
title_full_unstemmed Marine bioprospecting: enzymes and stress proteins from the sea anemones Anthopleura dowii and Lebrunia neglecta
title_sort Marine bioprospecting: enzymes and stress proteins from the sea anemones Anthopleura dowii and Lebrunia neglecta
dc.creator.none.fl_str_mv Ramírez Carreto, Santos
Miranda Zaragoza, Beatriz
Simões, Nuno
González Muñoz, Ricardo Enrique
Rodríguez Almazan, Claudia
author Ramírez Carreto, Santos
author_facet Ramírez Carreto, Santos
Miranda Zaragoza, Beatriz
Simões, Nuno
González Muñoz, Ricardo Enrique
Rodríguez Almazan, Claudia
author_role author
author2 Miranda Zaragoza, Beatriz
Simões, Nuno
González Muñoz, Ricardo Enrique
Rodríguez Almazan, Claudia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Lebrunia neglecta
Antopleura dowii
proteome
crude venom extract
enzymes
sea anemone
topic Lebrunia neglecta
Antopleura dowii
proteome
crude venom extract
enzymes
sea anemone
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The bioprospecting of sea anemone tissues and secretions has revealed that they are naturallibraries of polypeptides with diverse biological activities that can be utilized to develop ofbiotechnological tools with potential medical and industrial applications. This study conducteda proteomic analysis of crude venom extracts from Anthopleura dowii Verrill, 1869, and Lebrunianeglecta Duchassaing & Michelotti, 1860. The obtained data allowed us to identify 201 polypeptides,of which 39% were present in both extracts. Among the obtained sequences, hydrolase-type enzymes,oxidoreductases, transferases, heat shock proteins, adhesion proteins, and protease inhibitors,among others, were identified. Interaction analysis and functional annotation indicated thatthese proteins are primarily involved in endoplasmic reticulum metabolic processes such ascarbon metabolism and protein processing. In addition, several proteins related to oxidative stresswere identified, including superoxide dismutase, peroxiredoxins, thioredoxin, and glutathione oxidase.Our results provide novel information on the polypeptide composition of the crude venomextract from sea anemones, which can be utilized to develop molecules for therapeutic tools andindustrial applications.
Fil: Ramírez Carreto, Santos. Instituto Nacional de Salud Pública; México
Fil: Miranda Zaragoza, Beatriz. Universidad Nacional Autónoma de México; México
Fil: Simões, Nuno. Universidad Nacional Autónoma de México; México. Texas A and M University-Corpus Christi; Estados Unidos
Fil: González Muñoz, Ricardo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Rodríguez Almazan, Claudia. Universidad Nacional Autónoma de México; México
description The bioprospecting of sea anemone tissues and secretions has revealed that they are naturallibraries of polypeptides with diverse biological activities that can be utilized to develop ofbiotechnological tools with potential medical and industrial applications. This study conducteda proteomic analysis of crude venom extracts from Anthopleura dowii Verrill, 1869, and Lebrunianeglecta Duchassaing & Michelotti, 1860. The obtained data allowed us to identify 201 polypeptides,of which 39% were present in both extracts. Among the obtained sequences, hydrolase-type enzymes,oxidoreductases, transferases, heat shock proteins, adhesion proteins, and protease inhibitors,among others, were identified. Interaction analysis and functional annotation indicated thatthese proteins are primarily involved in endoplasmic reticulum metabolic processes such ascarbon metabolism and protein processing. In addition, several proteins related to oxidative stresswere identified, including superoxide dismutase, peroxiredoxins, thioredoxin, and glutathione oxidase.Our results provide novel information on the polypeptide composition of the crude venomextract from sea anemones, which can be utilized to develop molecules for therapeutic tools andindustrial applications.
publishDate 2023
dc.date.none.fl_str_mv 2023-12-23
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/234284
Ramírez Carreto, Santos; Miranda Zaragoza, Beatriz; Simões, Nuno; González Muñoz, Ricardo Enrique; Rodríguez Almazan, Claudia; Marine bioprospecting: enzymes and stress proteins from the sea anemones Anthopleura dowii and Lebrunia neglecta; Molecular Diversity Preservation International; Marine Drugs; 22; 1; 23-12-2023; 1-25
1660-3397
CONICET Digital
CONICET
url http://hdl.handle.net/11336/234284
identifier_str_mv Ramírez Carreto, Santos; Miranda Zaragoza, Beatriz; Simões, Nuno; González Muñoz, Ricardo Enrique; Rodríguez Almazan, Claudia; Marine bioprospecting: enzymes and stress proteins from the sea anemones Anthopleura dowii and Lebrunia neglecta; Molecular Diversity Preservation International; Marine Drugs; 22; 1; 23-12-2023; 1-25
1660-3397
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1660-3397/22/1/12
info:eu-repo/semantics/altIdentifier/doi/10.3390/md22010012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083220258422784
score 13.22299

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