1α,25(OH)2D3 and parathyroid hormone (PTH) signaling in rat intestinal cells: Activation of cytosolic PLA2

Autores
Gentili, Claudia Rosana; Morelli, Susana Ana; Russo de Boland, Ana
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the current study, we have probed the role of cytosolic phospholipase A2 (cPLA2) activity in the cellular response to the calciotropic hormones, 1α,25,dihydroxy-vitamin D3 [1α,25(OH)2D 3] and PTH. Stimulation of rat enterocytes with either hormone, increased release of arachidonic acid (AA) [3H-AA] one-two fold in a concentration and time-dependent manner. The effect of either hormone on enterocytes was totally reduced by preincubation with the intracellular Ca 2+ chelator BAPTA-AM (5 μM), suggesting that the release of AA following cell exposure to the calciotropic hormones occurs mainly through a Ca2+-dependent mechanism involving activation of Ca 2+-dependent cPLA2. Calciotropic homone stimulation of rat intestinal cells increases cPLA2 phosphorylation (three to four fold). This effect was decreased by PD 98059 (20 μM), a MAP kinase inhibitor, indicating that this action is, in part, mediated through activation of the MAP kinases ERK 1 and ERK2. Enterocytes exposure to 1α,25(OH)2D3 (1 nM) or PTH (10 nM) also resulted in P-cPLA2 translocation from cytosol to nuclei and membrane fractions, where phospholipase subtrates reside. Collectively, these data suggest that PTH and 1α,25(OH)2D3 activate in duodenal cells, a Ca2+-dependent cytosolic PLA2 and attendant arachidonic acid release and that this activation requieres prior stimulation of intracellular ERK1/2. 1α,25(OH)2D3 and PTH modulation of cPLA2 activity may change membrane fluidity and permeability and thereby affecting intestinal cell membrane function.
Fil: Gentili, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Morelli, Susana Ana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Russo de Boland, Ana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Materia
1Α,25,DIHYDROXY-VITAMIN D3
ARACHIDONIC ACID
CPLA2
PTH
RAT ENTEROCYTES
SIGNAL TRANSDUCTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/97985
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/97985
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling 1α,25(OH)2D3 and parathyroid hormone (PTH) signaling in rat intestinal cells: Activation of cytosolic PLA2Gentili, Claudia RosanaMorelli, Susana AnaRusso de Boland, Ana1Α,25,DIHYDROXY-VITAMIN D3ARACHIDONIC ACIDCPLA2PTHRAT ENTEROCYTESSIGNAL TRANSDUCTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the current study, we have probed the role of cytosolic phospholipase A2 (cPLA2) activity in the cellular response to the calciotropic hormones, 1α,25,dihydroxy-vitamin D3 [1α,25(OH)2D 3] and PTH. Stimulation of rat enterocytes with either hormone, increased release of arachidonic acid (AA) [3H-AA] one-two fold in a concentration and time-dependent manner. The effect of either hormone on enterocytes was totally reduced by preincubation with the intracellular Ca 2+ chelator BAPTA-AM (5 μM), suggesting that the release of AA following cell exposure to the calciotropic hormones occurs mainly through a Ca2+-dependent mechanism involving activation of Ca 2+-dependent cPLA2. Calciotropic homone stimulation of rat intestinal cells increases cPLA2 phosphorylation (three to four fold). This effect was decreased by PD 98059 (20 μM), a MAP kinase inhibitor, indicating that this action is, in part, mediated through activation of the MAP kinases ERK 1 and ERK2. Enterocytes exposure to 1α,25(OH)2D3 (1 nM) or PTH (10 nM) also resulted in P-cPLA2 translocation from cytosol to nuclei and membrane fractions, where phospholipase subtrates reside. Collectively, these data suggest that PTH and 1α,25(OH)2D3 activate in duodenal cells, a Ca2+-dependent cytosolic PLA2 and attendant arachidonic acid release and that this activation requieres prior stimulation of intracellular ERK1/2. 1α,25(OH)2D3 and PTH modulation of cPLA2 activity may change membrane fluidity and permeability and thereby affecting intestinal cell membrane function.Fil: Gentili, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Morelli, Susana Ana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Russo de Boland, Ana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaPergamon-Elsevier Science Ltd2004-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97985Gentili, Claudia Rosana; Morelli, Susana Ana; Russo de Boland, Ana; 1α,25(OH)2D3 and parathyroid hormone (PTH) signaling in rat intestinal cells: Activation of cytosolic PLA2; Pergamon-Elsevier Science Ltd; Journal of Steroid Biochemistry and Molecular Biology; 89-90; 5-2004; 297-3010960-0760CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0960076004000780info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jsbmb.2004.03.030info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:23Zoai:ri.conicet.gov.ar:11336/97985instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:23.358CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv 1α,25(OH)2D3 and parathyroid hormone (PTH) signaling in rat intestinal cells: Activation of cytosolic PLA2
title 1α,25(OH)2D3 and parathyroid hormone (PTH) signaling in rat intestinal cells: Activation of cytosolic PLA2
spellingShingle 1α,25(OH)2D3 and parathyroid hormone (PTH) signaling in rat intestinal cells: Activation of cytosolic PLA2
Gentili, Claudia Rosana
1Α,25,DIHYDROXY-VITAMIN D3
ARACHIDONIC ACID
CPLA2
PTH
RAT ENTEROCYTES
SIGNAL TRANSDUCTION
title_short 1α,25(OH)2D3 and parathyroid hormone (PTH) signaling in rat intestinal cells: Activation of cytosolic PLA2
title_full 1α,25(OH)2D3 and parathyroid hormone (PTH) signaling in rat intestinal cells: Activation of cytosolic PLA2
title_fullStr 1α,25(OH)2D3 and parathyroid hormone (PTH) signaling in rat intestinal cells: Activation of cytosolic PLA2
title_full_unstemmed 1α,25(OH)2D3 and parathyroid hormone (PTH) signaling in rat intestinal cells: Activation of cytosolic PLA2
title_sort 1α,25(OH)2D3 and parathyroid hormone (PTH) signaling in rat intestinal cells: Activation of cytosolic PLA2
dc.creator.none.fl_str_mv Gentili, Claudia Rosana
Morelli, Susana Ana
Russo de Boland, Ana
author Gentili, Claudia Rosana
author_facet Gentili, Claudia Rosana
Morelli, Susana Ana
Russo de Boland, Ana
author_role author
author2 Morelli, Susana Ana
Russo de Boland, Ana
author2_role author
author
dc.subject.none.fl_str_mv 1Α,25,DIHYDROXY-VITAMIN D3
ARACHIDONIC ACID
CPLA2
PTH
RAT ENTEROCYTES
SIGNAL TRANSDUCTION
topic 1Α,25,DIHYDROXY-VITAMIN D3
ARACHIDONIC ACID
CPLA2
PTH
RAT ENTEROCYTES
SIGNAL TRANSDUCTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the current study, we have probed the role of cytosolic phospholipase A2 (cPLA2) activity in the cellular response to the calciotropic hormones, 1α,25,dihydroxy-vitamin D3 [1α,25(OH)2D 3] and PTH. Stimulation of rat enterocytes with either hormone, increased release of arachidonic acid (AA) [3H-AA] one-two fold in a concentration and time-dependent manner. The effect of either hormone on enterocytes was totally reduced by preincubation with the intracellular Ca 2+ chelator BAPTA-AM (5 μM), suggesting that the release of AA following cell exposure to the calciotropic hormones occurs mainly through a Ca2+-dependent mechanism involving activation of Ca 2+-dependent cPLA2. Calciotropic homone stimulation of rat intestinal cells increases cPLA2 phosphorylation (three to four fold). This effect was decreased by PD 98059 (20 μM), a MAP kinase inhibitor, indicating that this action is, in part, mediated through activation of the MAP kinases ERK 1 and ERK2. Enterocytes exposure to 1α,25(OH)2D3 (1 nM) or PTH (10 nM) also resulted in P-cPLA2 translocation from cytosol to nuclei and membrane fractions, where phospholipase subtrates reside. Collectively, these data suggest that PTH and 1α,25(OH)2D3 activate in duodenal cells, a Ca2+-dependent cytosolic PLA2 and attendant arachidonic acid release and that this activation requieres prior stimulation of intracellular ERK1/2. 1α,25(OH)2D3 and PTH modulation of cPLA2 activity may change membrane fluidity and permeability and thereby affecting intestinal cell membrane function.
Fil: Gentili, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Morelli, Susana Ana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Russo de Boland, Ana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
description In the current study, we have probed the role of cytosolic phospholipase A2 (cPLA2) activity in the cellular response to the calciotropic hormones, 1α,25,dihydroxy-vitamin D3 [1α,25(OH)2D 3] and PTH. Stimulation of rat enterocytes with either hormone, increased release of arachidonic acid (AA) [3H-AA] one-two fold in a concentration and time-dependent manner. The effect of either hormone on enterocytes was totally reduced by preincubation with the intracellular Ca 2+ chelator BAPTA-AM (5 μM), suggesting that the release of AA following cell exposure to the calciotropic hormones occurs mainly through a Ca2+-dependent mechanism involving activation of Ca 2+-dependent cPLA2. Calciotropic homone stimulation of rat intestinal cells increases cPLA2 phosphorylation (three to four fold). This effect was decreased by PD 98059 (20 μM), a MAP kinase inhibitor, indicating that this action is, in part, mediated through activation of the MAP kinases ERK 1 and ERK2. Enterocytes exposure to 1α,25(OH)2D3 (1 nM) or PTH (10 nM) also resulted in P-cPLA2 translocation from cytosol to nuclei and membrane fractions, where phospholipase subtrates reside. Collectively, these data suggest that PTH and 1α,25(OH)2D3 activate in duodenal cells, a Ca2+-dependent cytosolic PLA2 and attendant arachidonic acid release and that this activation requieres prior stimulation of intracellular ERK1/2. 1α,25(OH)2D3 and PTH modulation of cPLA2 activity may change membrane fluidity and permeability and thereby affecting intestinal cell membrane function.
publishDate 2004
dc.date.none.fl_str_mv 2004-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/97985
Gentili, Claudia Rosana; Morelli, Susana Ana; Russo de Boland, Ana; 1α,25(OH)2D3 and parathyroid hormone (PTH) signaling in rat intestinal cells: Activation of cytosolic PLA2; Pergamon-Elsevier Science Ltd; Journal of Steroid Biochemistry and Molecular Biology; 89-90; 5-2004; 297-301
0960-0760
CONICET Digital
CONICET
url http://hdl.handle.net/11336/97985
identifier_str_mv Gentili, Claudia Rosana; Morelli, Susana Ana; Russo de Boland, Ana; 1α,25(OH)2D3 and parathyroid hormone (PTH) signaling in rat intestinal cells: Activation of cytosolic PLA2; Pergamon-Elsevier Science Ltd; Journal of Steroid Biochemistry and Molecular Biology; 89-90; 5-2004; 297-301
0960-0760
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0960076004000780
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jsbmb.2004.03.030
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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