Expression of genes involved in testosterone and 17β-estradiol production in ex vivo cultured prepuberal mouse testes

Autores
Tajes Ardanaz, Oliverio Julian; Luquez, Jessica Mariela; Sanchez Chaves, Manuela; Oresti, Gerardo Martin
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
A highly efficient testicular culture system involving a gas-liquid interface has emerged. This method has demonstrated the ability, although a low rate, to differentiate male germ cells from neonatal spermatogonia to haploid cells. However, the model requires thorough examination to facilitate forthcoming experimental approaches in physiological and toxicological reproductive studies. Sequential male germ cell proliferation and differentiation requires the production and the action of steroid hormones. Consequently, our aims were to record production levels of testosterone (Tes) and 17β-estradiol (E2) and to examine the expression of genes encoding proteins that are involved in such hormone production in ex vivo cultured testes. The mRNA expression patterns of 17β-Hydroxysteroid dehydrogenase 1 (17β-Hsd1), 3β-Hydroxysteroid dehydrogenase (3β-Hsd1), Sex hormone binding globulin (Shbg) and Cytochrome P450-Aromatase (Cyp19a1) were followed in 6.5 days old C57BL/6J mouse testis explants cultured during 44 days and were compared to those recorded during in vivo development. Notoriously, 17β-Hsd1 and 3β-Hsd1 had a similar expression pattern ex vivo vs. in vivo, but the levels of Tes decreased from 5 to 20 days in culture and thereafter remained low. Concomitantly, the mRNA expression of Shbg was up-regulated with time in the explants, linked to the low Tes production. Finally, as in vivo, Cyp19a1 expression tended to increase from days 10 to 30, while the E2 levels recorded from 15 to 20 days were the highest. The latter was associated with the appearance of haploid spermatids. Interestingly, when retinoic acid was added to the media, the levels of Tes were increased and mRNA expression levels of 17β-Hsd1 and 3β-Hsd1 were up-regulated, while those of Shbg were down-regulated. Our results demonstrated active production of steroid hormones in the explants and suggest that modulation of these hormones could enhance the ex vivo spermatogenesis process. Supported by SGCyT UNS-PGI-UNS [24/B272 to GMO], FONCyT, [PICT2020- 02056 to GMO] and SGCyT UNS-PGI 24/B341 to GMO and JML.
Fil: Tajes Ardanaz, Oliverio Julian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Luquez, Jessica Mariela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Sanchez Chaves, Manuela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LIX Reunión Anual de la Sociedad Argentina de Bioquímica y Biología Molecular
Rosario
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
Steroid hormone
testosterone
spermatogenesis
estrogen
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/232476
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/232476
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression of genes involved in testosterone and 17β-estradiol production in ex vivo cultured prepuberal mouse testesTajes Ardanaz, Oliverio JulianLuquez, Jessica MarielaSanchez Chaves, ManuelaOresti, Gerardo MartinSteroid hormonetestosteronespermatogenesisestrogenhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A highly efficient testicular culture system involving a gas-liquid interface has emerged. This method has demonstrated the ability, although a low rate, to differentiate male germ cells from neonatal spermatogonia to haploid cells. However, the model requires thorough examination to facilitate forthcoming experimental approaches in physiological and toxicological reproductive studies. Sequential male germ cell proliferation and differentiation requires the production and the action of steroid hormones. Consequently, our aims were to record production levels of testosterone (Tes) and 17β-estradiol (E2) and to examine the expression of genes encoding proteins that are involved in such hormone production in ex vivo cultured testes. The mRNA expression patterns of 17β-Hydroxysteroid dehydrogenase 1 (17β-Hsd1), 3β-Hydroxysteroid dehydrogenase (3β-Hsd1), Sex hormone binding globulin (Shbg) and Cytochrome P450-Aromatase (Cyp19a1) were followed in 6.5 days old C57BL/6J mouse testis explants cultured during 44 days and were compared to those recorded during in vivo development. Notoriously, 17β-Hsd1 and 3β-Hsd1 had a similar expression pattern ex vivo vs. in vivo, but the levels of Tes decreased from 5 to 20 days in culture and thereafter remained low. Concomitantly, the mRNA expression of Shbg was up-regulated with time in the explants, linked to the low Tes production. Finally, as in vivo, Cyp19a1 expression tended to increase from days 10 to 30, while the E2 levels recorded from 15 to 20 days were the highest. The latter was associated with the appearance of haploid spermatids. Interestingly, when retinoic acid was added to the media, the levels of Tes were increased and mRNA expression levels of 17β-Hsd1 and 3β-Hsd1 were up-regulated, while those of Shbg were down-regulated. Our results demonstrated active production of steroid hormones in the explants and suggest that modulation of these hormones could enhance the ex vivo spermatogenesis process. Supported by SGCyT UNS-PGI-UNS [24/B272 to GMO], FONCyT, [PICT2020- 02056 to GMO] and SGCyT UNS-PGI 24/B341 to GMO and JML.Fil: Tajes Ardanaz, Oliverio Julian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Luquez, Jessica Mariela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Sanchez Chaves, Manuela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLIX Reunión Anual de la Sociedad Argentina de Bioquímica y Biología MolecularRosarioArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularSociedad Argentina de Investigación en Bioquímica y Biología Molecular2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/232476Expression of genes involved in testosterone and 17β-estradiol production in ex vivo cultured prepuberal mouse testes; LIX Reunión Anual de la Sociedad Argentina de Bioquímica y Biología Molecular; Rosario; Argentina; 2023; 231-232CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/2023/abstracts-EN.pdfinfo:eu-repo/semantics/altIdentifier/url/https://newsite.saib.org.ar/congreso-2023/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:23Zoai:ri.conicet.gov.ar:11336/232476instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:23.504CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression of genes involved in testosterone and 17β-estradiol production in ex vivo cultured prepuberal mouse testes
title Expression of genes involved in testosterone and 17β-estradiol production in ex vivo cultured prepuberal mouse testes
spellingShingle Expression of genes involved in testosterone and 17β-estradiol production in ex vivo cultured prepuberal mouse testes
Tajes Ardanaz, Oliverio Julian
Steroid hormone
testosterone
spermatogenesis
estrogen
title_short Expression of genes involved in testosterone and 17β-estradiol production in ex vivo cultured prepuberal mouse testes
title_full Expression of genes involved in testosterone and 17β-estradiol production in ex vivo cultured prepuberal mouse testes
title_fullStr Expression of genes involved in testosterone and 17β-estradiol production in ex vivo cultured prepuberal mouse testes
title_full_unstemmed Expression of genes involved in testosterone and 17β-estradiol production in ex vivo cultured prepuberal mouse testes
title_sort Expression of genes involved in testosterone and 17β-estradiol production in ex vivo cultured prepuberal mouse testes
dc.creator.none.fl_str_mv Tajes Ardanaz, Oliverio Julian
Luquez, Jessica Mariela
Sanchez Chaves, Manuela
Oresti, Gerardo Martin
author Tajes Ardanaz, Oliverio Julian
author_facet Tajes Ardanaz, Oliverio Julian
Luquez, Jessica Mariela
Sanchez Chaves, Manuela
Oresti, Gerardo Martin
author_role author
author2 Luquez, Jessica Mariela
Sanchez Chaves, Manuela
Oresti, Gerardo Martin
author2_role author
author
author
dc.subject.none.fl_str_mv Steroid hormone
testosterone
spermatogenesis
estrogen
topic Steroid hormone
testosterone
spermatogenesis
estrogen
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A highly efficient testicular culture system involving a gas-liquid interface has emerged. This method has demonstrated the ability, although a low rate, to differentiate male germ cells from neonatal spermatogonia to haploid cells. However, the model requires thorough examination to facilitate forthcoming experimental approaches in physiological and toxicological reproductive studies. Sequential male germ cell proliferation and differentiation requires the production and the action of steroid hormones. Consequently, our aims were to record production levels of testosterone (Tes) and 17β-estradiol (E2) and to examine the expression of genes encoding proteins that are involved in such hormone production in ex vivo cultured testes. The mRNA expression patterns of 17β-Hydroxysteroid dehydrogenase 1 (17β-Hsd1), 3β-Hydroxysteroid dehydrogenase (3β-Hsd1), Sex hormone binding globulin (Shbg) and Cytochrome P450-Aromatase (Cyp19a1) were followed in 6.5 days old C57BL/6J mouse testis explants cultured during 44 days and were compared to those recorded during in vivo development. Notoriously, 17β-Hsd1 and 3β-Hsd1 had a similar expression pattern ex vivo vs. in vivo, but the levels of Tes decreased from 5 to 20 days in culture and thereafter remained low. Concomitantly, the mRNA expression of Shbg was up-regulated with time in the explants, linked to the low Tes production. Finally, as in vivo, Cyp19a1 expression tended to increase from days 10 to 30, while the E2 levels recorded from 15 to 20 days were the highest. The latter was associated with the appearance of haploid spermatids. Interestingly, when retinoic acid was added to the media, the levels of Tes were increased and mRNA expression levels of 17β-Hsd1 and 3β-Hsd1 were up-regulated, while those of Shbg were down-regulated. Our results demonstrated active production of steroid hormones in the explants and suggest that modulation of these hormones could enhance the ex vivo spermatogenesis process. Supported by SGCyT UNS-PGI-UNS [24/B272 to GMO], FONCyT, [PICT2020- 02056 to GMO] and SGCyT UNS-PGI 24/B341 to GMO and JML.
Fil: Tajes Ardanaz, Oliverio Julian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Luquez, Jessica Mariela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Sanchez Chaves, Manuela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LIX Reunión Anual de la Sociedad Argentina de Bioquímica y Biología Molecular
Rosario
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description A highly efficient testicular culture system involving a gas-liquid interface has emerged. This method has demonstrated the ability, although a low rate, to differentiate male germ cells from neonatal spermatogonia to haploid cells. However, the model requires thorough examination to facilitate forthcoming experimental approaches in physiological and toxicological reproductive studies. Sequential male germ cell proliferation and differentiation requires the production and the action of steroid hormones. Consequently, our aims were to record production levels of testosterone (Tes) and 17β-estradiol (E2) and to examine the expression of genes encoding proteins that are involved in such hormone production in ex vivo cultured testes. The mRNA expression patterns of 17β-Hydroxysteroid dehydrogenase 1 (17β-Hsd1), 3β-Hydroxysteroid dehydrogenase (3β-Hsd1), Sex hormone binding globulin (Shbg) and Cytochrome P450-Aromatase (Cyp19a1) were followed in 6.5 days old C57BL/6J mouse testis explants cultured during 44 days and were compared to those recorded during in vivo development. Notoriously, 17β-Hsd1 and 3β-Hsd1 had a similar expression pattern ex vivo vs. in vivo, but the levels of Tes decreased from 5 to 20 days in culture and thereafter remained low. Concomitantly, the mRNA expression of Shbg was up-regulated with time in the explants, linked to the low Tes production. Finally, as in vivo, Cyp19a1 expression tended to increase from days 10 to 30, while the E2 levels recorded from 15 to 20 days were the highest. The latter was associated with the appearance of haploid spermatids. Interestingly, when retinoic acid was added to the media, the levels of Tes were increased and mRNA expression levels of 17β-Hsd1 and 3β-Hsd1 were up-regulated, while those of Shbg were down-regulated. Our results demonstrated active production of steroid hormones in the explants and suggest that modulation of these hormones could enhance the ex vivo spermatogenesis process. Supported by SGCyT UNS-PGI-UNS [24/B272 to GMO], FONCyT, [PICT2020- 02056 to GMO] and SGCyT UNS-PGI 24/B341 to GMO and JML.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/232476
Expression of genes involved in testosterone and 17β-estradiol production in ex vivo cultured prepuberal mouse testes; LIX Reunión Anual de la Sociedad Argentina de Bioquímica y Biología Molecular; Rosario; Argentina; 2023; 231-232
CONICET Digital
CONICET
url http://hdl.handle.net/11336/232476
identifier_str_mv Expression of genes involved in testosterone and 17β-estradiol production in ex vivo cultured prepuberal mouse testes; LIX Reunión Anual de la Sociedad Argentina de Bioquímica y Biología Molecular; Rosario; Argentina; 2023; 231-232
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/https://newsite.saib.org.ar/congreso-2023/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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