Taxonomic analysis of white gypsum-halite precipitations from Laguna Verde
- Autores
- Marcelino, Virginia; Saracho, Hayde; Kurth, Daniel German
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The Argentine Puna represents a unique environment, characterized by high UV radiation, low oxygen pressure and extreme temperature fluctuations. There we find saline water deposits such as Laguna Verde (Salar de Antofalla, 3300 m.a.s.l.) surrounded by large extensions of white gypsum-halite precipitating crusts. These crusts harbor microbial communities distributed in layers (microbial mats) defined by physicochemical requirements, light and oxygen. These mats colonize both solid and sedimentary surfaces.Metagenomic, physiological and geochemical studies reveal a series of strategies that allow these communities to survive in hypersaline wetlands by performing photosynthesis and serving as CO2 sinks. The goal of this job was to determine the biodiversity associated to these large evaporitics biofilms surrounding the lake. It was observed that the microorganisms are organized in two layers in the salt crust, an upper yellow layer, and a lower green layer. Samples were taken using a cut off sterile 10-ml syringe, were fixed immediately in RNAlater and transported to the laboratory on ice. Total genomic DNA was isolated from each of the layers using the FastDNA ™ SPIN Kit for Soil (MP Biomedical) and it sequenced with Illumina HiSeq. Raw data obtained, was uploaded to the European Bioinformatic Archive (ENA). Once uploaded, it was analyzed using the MGnify Annotation Pipeline developed by Dr. Rob Finn’s Team at EMBL-EBI. This pipeline performed the taxonomic annotation of the raw reads.The taxonomic annotation obtained, showed that the orange layer is dominated by oxygenic photoautotrophic bacteria (Cyanobacteria, ca. 65%) followed by anoxygenic photoautotrophs (Proteobacteria, ca. 10%) from the classes Alphaproteobacteria and Gammaproteobacteria and heterotrophic bacteria (Bacteoridetes, ca. 10%). The green layer is dominated by the phyla Proteobacteria, ca. 50% followed by Bacteroidetes, ca. 30% and Cyanobacteria, ca. 20%. In both layers, the phyla Actinobacteria and Euryarcheota represent at least 1% of the relative abundance.
Fil: Marcelino, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Saracho, Hayde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Kurth, Daniel German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
7mo Simposio Argentino de Jóvenes Investigadores en Bioinformática
San Miguel de Tucumán
Argentina
Argentine Regional Student Group
Universidad Nacional de Tucumán. Facultad de Bioquímica Química y Farmacia - Materia
-
TAXONOMY
METAGENOMICS
SALT CRUST
SALINE LAKE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/213139
Ver los metadatos del registro completo
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Taxonomic analysis of white gypsum-halite precipitations from Laguna VerdeMarcelino, VirginiaSaracho, HaydeKurth, Daniel GermanTAXONOMYMETAGENOMICSSALT CRUSTSALINE LAKEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Argentine Puna represents a unique environment, characterized by high UV radiation, low oxygen pressure and extreme temperature fluctuations. There we find saline water deposits such as Laguna Verde (Salar de Antofalla, 3300 m.a.s.l.) surrounded by large extensions of white gypsum-halite precipitating crusts. These crusts harbor microbial communities distributed in layers (microbial mats) defined by physicochemical requirements, light and oxygen. These mats colonize both solid and sedimentary surfaces.Metagenomic, physiological and geochemical studies reveal a series of strategies that allow these communities to survive in hypersaline wetlands by performing photosynthesis and serving as CO2 sinks. The goal of this job was to determine the biodiversity associated to these large evaporitics biofilms surrounding the lake. It was observed that the microorganisms are organized in two layers in the salt crust, an upper yellow layer, and a lower green layer. Samples were taken using a cut off sterile 10-ml syringe, were fixed immediately in RNAlater and transported to the laboratory on ice. Total genomic DNA was isolated from each of the layers using the FastDNA ™ SPIN Kit for Soil (MP Biomedical) and it sequenced with Illumina HiSeq. Raw data obtained, was uploaded to the European Bioinformatic Archive (ENA). Once uploaded, it was analyzed using the MGnify Annotation Pipeline developed by Dr. Rob Finn’s Team at EMBL-EBI. This pipeline performed the taxonomic annotation of the raw reads.The taxonomic annotation obtained, showed that the orange layer is dominated by oxygenic photoautotrophic bacteria (Cyanobacteria, ca. 65%) followed by anoxygenic photoautotrophs (Proteobacteria, ca. 10%) from the classes Alphaproteobacteria and Gammaproteobacteria and heterotrophic bacteria (Bacteoridetes, ca. 10%). The green layer is dominated by the phyla Proteobacteria, ca. 50% followed by Bacteroidetes, ca. 30% and Cyanobacteria, ca. 20%. In both layers, the phyla Actinobacteria and Euryarcheota represent at least 1% of the relative abundance.Fil: Marcelino, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Saracho, Hayde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Kurth, Daniel German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina7mo Simposio Argentino de Jóvenes Investigadores en BioinformáticaSan Miguel de TucumánArgentinaArgentine Regional Student GroupUniversidad Nacional de Tucumán. Facultad de Bioquímica Química y FarmaciaArgentine Regional Student Group2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectSimposioBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213139Taxonomic analysis of white gypsum-halite precipitations from Laguna Verde; 7mo Simposio Argentino de Jóvenes Investigadores en Bioinformática; San Miguel de Tucumán; Argentina; 2022; 15-15CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://zenodo.org/record/7266042info:eu-repo/semantics/altIdentifier/doi/10.5281/zenodo.7266042Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:05Zoai:ri.conicet.gov.ar:11336/213139instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:06.261CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Taxonomic analysis of white gypsum-halite precipitations from Laguna Verde |
title |
Taxonomic analysis of white gypsum-halite precipitations from Laguna Verde |
spellingShingle |
Taxonomic analysis of white gypsum-halite precipitations from Laguna Verde Marcelino, Virginia TAXONOMY METAGENOMICS SALT CRUST SALINE LAKE |
title_short |
Taxonomic analysis of white gypsum-halite precipitations from Laguna Verde |
title_full |
Taxonomic analysis of white gypsum-halite precipitations from Laguna Verde |
title_fullStr |
Taxonomic analysis of white gypsum-halite precipitations from Laguna Verde |
title_full_unstemmed |
Taxonomic analysis of white gypsum-halite precipitations from Laguna Verde |
title_sort |
Taxonomic analysis of white gypsum-halite precipitations from Laguna Verde |
dc.creator.none.fl_str_mv |
Marcelino, Virginia Saracho, Hayde Kurth, Daniel German |
author |
Marcelino, Virginia |
author_facet |
Marcelino, Virginia Saracho, Hayde Kurth, Daniel German |
author_role |
author |
author2 |
Saracho, Hayde Kurth, Daniel German |
author2_role |
author author |
dc.subject.none.fl_str_mv |
TAXONOMY METAGENOMICS SALT CRUST SALINE LAKE |
topic |
TAXONOMY METAGENOMICS SALT CRUST SALINE LAKE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The Argentine Puna represents a unique environment, characterized by high UV radiation, low oxygen pressure and extreme temperature fluctuations. There we find saline water deposits such as Laguna Verde (Salar de Antofalla, 3300 m.a.s.l.) surrounded by large extensions of white gypsum-halite precipitating crusts. These crusts harbor microbial communities distributed in layers (microbial mats) defined by physicochemical requirements, light and oxygen. These mats colonize both solid and sedimentary surfaces.Metagenomic, physiological and geochemical studies reveal a series of strategies that allow these communities to survive in hypersaline wetlands by performing photosynthesis and serving as CO2 sinks. The goal of this job was to determine the biodiversity associated to these large evaporitics biofilms surrounding the lake. It was observed that the microorganisms are organized in two layers in the salt crust, an upper yellow layer, and a lower green layer. Samples were taken using a cut off sterile 10-ml syringe, were fixed immediately in RNAlater and transported to the laboratory on ice. Total genomic DNA was isolated from each of the layers using the FastDNA ™ SPIN Kit for Soil (MP Biomedical) and it sequenced with Illumina HiSeq. Raw data obtained, was uploaded to the European Bioinformatic Archive (ENA). Once uploaded, it was analyzed using the MGnify Annotation Pipeline developed by Dr. Rob Finn’s Team at EMBL-EBI. This pipeline performed the taxonomic annotation of the raw reads.The taxonomic annotation obtained, showed that the orange layer is dominated by oxygenic photoautotrophic bacteria (Cyanobacteria, ca. 65%) followed by anoxygenic photoautotrophs (Proteobacteria, ca. 10%) from the classes Alphaproteobacteria and Gammaproteobacteria and heterotrophic bacteria (Bacteoridetes, ca. 10%). The green layer is dominated by the phyla Proteobacteria, ca. 50% followed by Bacteroidetes, ca. 30% and Cyanobacteria, ca. 20%. In both layers, the phyla Actinobacteria and Euryarcheota represent at least 1% of the relative abundance. Fil: Marcelino, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Saracho, Hayde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Kurth, Daniel German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina 7mo Simposio Argentino de Jóvenes Investigadores en Bioinformática San Miguel de Tucumán Argentina Argentine Regional Student Group Universidad Nacional de Tucumán. Facultad de Bioquímica Química y Farmacia |
description |
The Argentine Puna represents a unique environment, characterized by high UV radiation, low oxygen pressure and extreme temperature fluctuations. There we find saline water deposits such as Laguna Verde (Salar de Antofalla, 3300 m.a.s.l.) surrounded by large extensions of white gypsum-halite precipitating crusts. These crusts harbor microbial communities distributed in layers (microbial mats) defined by physicochemical requirements, light and oxygen. These mats colonize both solid and sedimentary surfaces.Metagenomic, physiological and geochemical studies reveal a series of strategies that allow these communities to survive in hypersaline wetlands by performing photosynthesis and serving as CO2 sinks. The goal of this job was to determine the biodiversity associated to these large evaporitics biofilms surrounding the lake. It was observed that the microorganisms are organized in two layers in the salt crust, an upper yellow layer, and a lower green layer. Samples were taken using a cut off sterile 10-ml syringe, were fixed immediately in RNAlater and transported to the laboratory on ice. Total genomic DNA was isolated from each of the layers using the FastDNA ™ SPIN Kit for Soil (MP Biomedical) and it sequenced with Illumina HiSeq. Raw data obtained, was uploaded to the European Bioinformatic Archive (ENA). Once uploaded, it was analyzed using the MGnify Annotation Pipeline developed by Dr. Rob Finn’s Team at EMBL-EBI. This pipeline performed the taxonomic annotation of the raw reads.The taxonomic annotation obtained, showed that the orange layer is dominated by oxygenic photoautotrophic bacteria (Cyanobacteria, ca. 65%) followed by anoxygenic photoautotrophs (Proteobacteria, ca. 10%) from the classes Alphaproteobacteria and Gammaproteobacteria and heterotrophic bacteria (Bacteoridetes, ca. 10%). The green layer is dominated by the phyla Proteobacteria, ca. 50% followed by Bacteroidetes, ca. 30% and Cyanobacteria, ca. 20%. In both layers, the phyla Actinobacteria and Euryarcheota represent at least 1% of the relative abundance. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Simposio Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/213139 Taxonomic analysis of white gypsum-halite precipitations from Laguna Verde; 7mo Simposio Argentino de Jóvenes Investigadores en Bioinformática; San Miguel de Tucumán; Argentina; 2022; 15-15 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/213139 |
identifier_str_mv |
Taxonomic analysis of white gypsum-halite precipitations from Laguna Verde; 7mo Simposio Argentino de Jóvenes Investigadores en Bioinformática; San Miguel de Tucumán; Argentina; 2022; 15-15 CONICET Digital CONICET |
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eng |
language |
eng |
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openAccess |
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application/pdf application/pdf application/pdf |
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Argentine Regional Student Group |
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Argentine Regional Student Group |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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