Bovine Herpesvirus-4-Based Vector Delivering Peste des Petits Ruminants Virus Hemagglutinin ORF Induces both Neutralizing Antibodies and Cytotoxic T Cell Responses

Autores
Macchi, Francesca; Rojas, José Manuel; Verna, Andrea Elizabeth; Sevilla, Noemí; Franceschi, Valentina; Tebaldi, Giulia; Cavirani, Sandro; Martín, Verónica; Donofrio, Gaetano
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Peste des Petits Ruminants Virus (PPRV) is an extremely infective morbillivirus thatprimarily affects goats and sheep. In underdeveloped countries where livestock are themain economical resource, PPRV causes considerable economic losses. Protective liveattenuated vaccines are currently available but they induce antibody responses similar tothose produced in PPRV naturally infected animals. Effective vaccines able to distinguishbetween vaccinated and naturally infected animals are required to PPRV control anderadication programs. Hemagglutinin (H) is a highly immunogenic PPRV envelope glycoproteindisplaying both hemagglutinin and neuraminidase activities, playing a crucial rolein virus attachment and penetration. In this study, a recombinant Bovine Herpesvirus-4(BoHV-4)-based vector delivering an optimized PPRV-Hemagglutinin expression cassette,BoHV-4-A-PPRV-H-ΔTK, was assessed in immunocompetent C57BL/6 mice.BoHV-4-A-PPRV-H-ΔTK-immunization elicited both cellular and humoral immuneresponses with specific T cell, cytotoxic T lymphocyte, and sero-neutralizing antibodyagainst PPRV. These data suggest recombinant BoHV-4-A-PPRV-H-ΔTK as an effectivevaccine candidate to protect against PPRV herd infection and potentially applicable foreradication programs.
Fil: Macchi, Francesca. Università di Parma; Italia
Fil: Rojas, José Manuel. Centro de Investigación en Sanidad Animal; España
Fil: Verna, Andrea Elizabeth. Università di Parma; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Sevilla, Noemí. Centro de Investigación en Sanidad Animal; España
Fil: Franceschi, Valentina. Università di Parma; Italia
Fil: Tebaldi, Giulia. Università di Parma; Italia
Fil: Cavirani, Sandro. Università di Parma; Italia
Fil: Martín, Verónica. Centro de Investigación en Sanidad Animal; España
Fil: Donofrio, Gaetano. Università di Parma; Italia
Materia
BOHV4
PPRV
DIVA VACCINES
H ANTIGEN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102553
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bovine Herpesvirus-4-Based Vector Delivering Peste des Petits Ruminants Virus Hemagglutinin ORF Induces both Neutralizing Antibodies and Cytotoxic T Cell ResponsesMacchi, FrancescaRojas, José ManuelVerna, Andrea ElizabethSevilla, NoemíFranceschi, ValentinaTebaldi, GiuliaCavirani, SandroMartín, VerónicaDonofrio, GaetanoBOHV4PPRVDIVA VACCINESH ANTIGENhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Peste des Petits Ruminants Virus (PPRV) is an extremely infective morbillivirus thatprimarily affects goats and sheep. In underdeveloped countries where livestock are themain economical resource, PPRV causes considerable economic losses. Protective liveattenuated vaccines are currently available but they induce antibody responses similar tothose produced in PPRV naturally infected animals. Effective vaccines able to distinguishbetween vaccinated and naturally infected animals are required to PPRV control anderadication programs. Hemagglutinin (H) is a highly immunogenic PPRV envelope glycoproteindisplaying both hemagglutinin and neuraminidase activities, playing a crucial rolein virus attachment and penetration. In this study, a recombinant Bovine Herpesvirus-4(BoHV-4)-based vector delivering an optimized PPRV-Hemagglutinin expression cassette,BoHV-4-A-PPRV-H-ΔTK, was assessed in immunocompetent C57BL/6 mice.BoHV-4-A-PPRV-H-ΔTK-immunization elicited both cellular and humoral immuneresponses with specific T cell, cytotoxic T lymphocyte, and sero-neutralizing antibodyagainst PPRV. These data suggest recombinant BoHV-4-A-PPRV-H-ΔTK as an effectivevaccine candidate to protect against PPRV herd infection and potentially applicable foreradication programs.Fil: Macchi, Francesca. Università di Parma; ItaliaFil: Rojas, José Manuel. Centro de Investigación en Sanidad Animal; EspañaFil: Verna, Andrea Elizabeth. Università di Parma; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Sevilla, Noemí. Centro de Investigación en Sanidad Animal; EspañaFil: Franceschi, Valentina. Università di Parma; ItaliaFil: Tebaldi, Giulia. Università di Parma; ItaliaFil: Cavirani, Sandro. Università di Parma; ItaliaFil: Martín, Verónica. Centro de Investigación en Sanidad Animal; EspañaFil: Donofrio, Gaetano. Università di Parma; ItaliaFrontiers Media S.A.2018-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102553Macchi, Francesca; Rojas, José Manuel; Verna, Andrea Elizabeth; Sevilla, Noemí; Franceschi, Valentina; et al.; Bovine Herpesvirus-4-Based Vector Delivering Peste des Petits Ruminants Virus Hemagglutinin ORF Induces both Neutralizing Antibodies and Cytotoxic T Cell Responses; Frontiers Media S.A.; Frontiers in Immunology; 9; 3-2018; 1-111664-3224CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fimmu.2018.00421/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2018.00421info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:07:08Zoai:ri.conicet.gov.ar:11336/102553instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:07:09.103CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bovine Herpesvirus-4-Based Vector Delivering Peste des Petits Ruminants Virus Hemagglutinin ORF Induces both Neutralizing Antibodies and Cytotoxic T Cell Responses
title Bovine Herpesvirus-4-Based Vector Delivering Peste des Petits Ruminants Virus Hemagglutinin ORF Induces both Neutralizing Antibodies and Cytotoxic T Cell Responses
spellingShingle Bovine Herpesvirus-4-Based Vector Delivering Peste des Petits Ruminants Virus Hemagglutinin ORF Induces both Neutralizing Antibodies and Cytotoxic T Cell Responses
Macchi, Francesca
BOHV4
PPRV
DIVA VACCINES
H ANTIGEN
title_short Bovine Herpesvirus-4-Based Vector Delivering Peste des Petits Ruminants Virus Hemagglutinin ORF Induces both Neutralizing Antibodies and Cytotoxic T Cell Responses
title_full Bovine Herpesvirus-4-Based Vector Delivering Peste des Petits Ruminants Virus Hemagglutinin ORF Induces both Neutralizing Antibodies and Cytotoxic T Cell Responses
title_fullStr Bovine Herpesvirus-4-Based Vector Delivering Peste des Petits Ruminants Virus Hemagglutinin ORF Induces both Neutralizing Antibodies and Cytotoxic T Cell Responses
title_full_unstemmed Bovine Herpesvirus-4-Based Vector Delivering Peste des Petits Ruminants Virus Hemagglutinin ORF Induces both Neutralizing Antibodies and Cytotoxic T Cell Responses
title_sort Bovine Herpesvirus-4-Based Vector Delivering Peste des Petits Ruminants Virus Hemagglutinin ORF Induces both Neutralizing Antibodies and Cytotoxic T Cell Responses
dc.creator.none.fl_str_mv Macchi, Francesca
Rojas, José Manuel
Verna, Andrea Elizabeth
Sevilla, Noemí
Franceschi, Valentina
Tebaldi, Giulia
Cavirani, Sandro
Martín, Verónica
Donofrio, Gaetano
author Macchi, Francesca
author_facet Macchi, Francesca
Rojas, José Manuel
Verna, Andrea Elizabeth
Sevilla, Noemí
Franceschi, Valentina
Tebaldi, Giulia
Cavirani, Sandro
Martín, Verónica
Donofrio, Gaetano
author_role author
author2 Rojas, José Manuel
Verna, Andrea Elizabeth
Sevilla, Noemí
Franceschi, Valentina
Tebaldi, Giulia
Cavirani, Sandro
Martín, Verónica
Donofrio, Gaetano
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BOHV4
PPRV
DIVA VACCINES
H ANTIGEN
topic BOHV4
PPRV
DIVA VACCINES
H ANTIGEN
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Peste des Petits Ruminants Virus (PPRV) is an extremely infective morbillivirus thatprimarily affects goats and sheep. In underdeveloped countries where livestock are themain economical resource, PPRV causes considerable economic losses. Protective liveattenuated vaccines are currently available but they induce antibody responses similar tothose produced in PPRV naturally infected animals. Effective vaccines able to distinguishbetween vaccinated and naturally infected animals are required to PPRV control anderadication programs. Hemagglutinin (H) is a highly immunogenic PPRV envelope glycoproteindisplaying both hemagglutinin and neuraminidase activities, playing a crucial rolein virus attachment and penetration. In this study, a recombinant Bovine Herpesvirus-4(BoHV-4)-based vector delivering an optimized PPRV-Hemagglutinin expression cassette,BoHV-4-A-PPRV-H-ΔTK, was assessed in immunocompetent C57BL/6 mice.BoHV-4-A-PPRV-H-ΔTK-immunization elicited both cellular and humoral immuneresponses with specific T cell, cytotoxic T lymphocyte, and sero-neutralizing antibodyagainst PPRV. These data suggest recombinant BoHV-4-A-PPRV-H-ΔTK as an effectivevaccine candidate to protect against PPRV herd infection and potentially applicable foreradication programs.
Fil: Macchi, Francesca. Università di Parma; Italia
Fil: Rojas, José Manuel. Centro de Investigación en Sanidad Animal; España
Fil: Verna, Andrea Elizabeth. Università di Parma; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Sevilla, Noemí. Centro de Investigación en Sanidad Animal; España
Fil: Franceschi, Valentina. Università di Parma; Italia
Fil: Tebaldi, Giulia. Università di Parma; Italia
Fil: Cavirani, Sandro. Università di Parma; Italia
Fil: Martín, Verónica. Centro de Investigación en Sanidad Animal; España
Fil: Donofrio, Gaetano. Università di Parma; Italia
description Peste des Petits Ruminants Virus (PPRV) is an extremely infective morbillivirus thatprimarily affects goats and sheep. In underdeveloped countries where livestock are themain economical resource, PPRV causes considerable economic losses. Protective liveattenuated vaccines are currently available but they induce antibody responses similar tothose produced in PPRV naturally infected animals. Effective vaccines able to distinguishbetween vaccinated and naturally infected animals are required to PPRV control anderadication programs. Hemagglutinin (H) is a highly immunogenic PPRV envelope glycoproteindisplaying both hemagglutinin and neuraminidase activities, playing a crucial rolein virus attachment and penetration. In this study, a recombinant Bovine Herpesvirus-4(BoHV-4)-based vector delivering an optimized PPRV-Hemagglutinin expression cassette,BoHV-4-A-PPRV-H-ΔTK, was assessed in immunocompetent C57BL/6 mice.BoHV-4-A-PPRV-H-ΔTK-immunization elicited both cellular and humoral immuneresponses with specific T cell, cytotoxic T lymphocyte, and sero-neutralizing antibodyagainst PPRV. These data suggest recombinant BoHV-4-A-PPRV-H-ΔTK as an effectivevaccine candidate to protect against PPRV herd infection and potentially applicable foreradication programs.
publishDate 2018
dc.date.none.fl_str_mv 2018-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102553
Macchi, Francesca; Rojas, José Manuel; Verna, Andrea Elizabeth; Sevilla, Noemí; Franceschi, Valentina; et al.; Bovine Herpesvirus-4-Based Vector Delivering Peste des Petits Ruminants Virus Hemagglutinin ORF Induces both Neutralizing Antibodies and Cytotoxic T Cell Responses; Frontiers Media S.A.; Frontiers in Immunology; 9; 3-2018; 1-11
1664-3224
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102553
identifier_str_mv Macchi, Francesca; Rojas, José Manuel; Verna, Andrea Elizabeth; Sevilla, Noemí; Franceschi, Valentina; et al.; Bovine Herpesvirus-4-Based Vector Delivering Peste des Petits Ruminants Virus Hemagglutinin ORF Induces both Neutralizing Antibodies and Cytotoxic T Cell Responses; Frontiers Media S.A.; Frontiers in Immunology; 9; 3-2018; 1-11
1664-3224
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fimmu.2018.00421/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2018.00421
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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