Erythrocyte nuclear size as a better diagnostic character than cell size in the identification of live cryptic polyploid species

Autores
Otero, Manuel Alejandro; Grenat, Pablo Raúl; Valetti, Julian Alonso; Salas, Nancy Edith; Martino, Adolfo Ludovico
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It is well documented in anurans the cryptic condition of many species complexes involving polyploids. In these complexes the character that clearly differentiates them is the number of chromosome complements. The blood cells of amphibians conserve their nucleus, and so the erythrocyte size is correlated with the DNA content. We analyzed two cryptic-polyploid complexes occurring in the center of Argentina: Odontophrynus cordobae (2n)/O. americanus (4n) and Pleurodema kriegi (4n)/P. cordobae (8n). Our aim was evaluate the efficiency in the utilization of nuclear area with respect to cellular area of the erythrocytes to define the limits values for the identification of cryptic-polyploid species. We studied 110 individuals of Pleurodema and 116 individuals of Odontoprhynus. For each individual, we measured the cellular and nuclear length (L) and width (A) of 40 erythrocytes (Area= L*A*π/4) and boundary values were calculated using distribution curves. In both complexes studied, the erythrometric parameters showed significant differences between related species. Moreover, in both complexes the nuclear area was more efficient for identifying the species (Pleurodema: 34.39 μm² (probability=99.96%) and Odontophrynus: 24.02 μm² (99.075%)) than the cell area (Pleurodema: 273.08 μm² (97.55%) y Odontophrynus: 197.69 μm² (97.94%)). Greater efficiency found using nuclear area is novel and significant because most studies use only the cell area to differentiate polyploid complexes.
Fil: Otero, Manuel Alejandro. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grenat, Pablo Raúl. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valetti, Julian Alonso. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salas, Nancy Edith. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martino, Adolfo Ludovico. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Erythrocyte
Nuclear Size
Cryptic Polyploid Species
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24055
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Erythrocyte nuclear size as a better diagnostic character than cell size in the identification of live cryptic polyploid speciesOtero, Manuel AlejandroGrenat, Pablo RaúlValetti, Julian AlonsoSalas, Nancy EdithMartino, Adolfo LudovicoErythrocyteNuclear SizeCryptic Polyploid Specieshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1It is well documented in anurans the cryptic condition of many species complexes involving polyploids. In these complexes the character that clearly differentiates them is the number of chromosome complements. The blood cells of amphibians conserve their nucleus, and so the erythrocyte size is correlated with the DNA content. We analyzed two cryptic-polyploid complexes occurring in the center of Argentina: Odontophrynus cordobae (2n)/O. americanus (4n) and Pleurodema kriegi (4n)/P. cordobae (8n). Our aim was evaluate the efficiency in the utilization of nuclear area with respect to cellular area of the erythrocytes to define the limits values for the identification of cryptic-polyploid species. We studied 110 individuals of Pleurodema and 116 individuals of Odontoprhynus. For each individual, we measured the cellular and nuclear length (L) and width (A) of 40 erythrocytes (Area= L*A*π/4) and boundary values were calculated using distribution curves. In both complexes studied, the erythrometric parameters showed significant differences between related species. Moreover, in both complexes the nuclear area was more efficient for identifying the species (Pleurodema: 34.39 μm² (probability=99.96%) and Odontophrynus: 24.02 μm² (99.075%)) than the cell area (Pleurodema: 273.08 μm² (97.55%) y Odontophrynus: 197.69 μm² (97.94%)). Greater efficiency found using nuclear area is novel and significant because most studies use only the cell area to differentiate polyploid complexes.Fil: Otero, Manuel Alejandro. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Grenat, Pablo Raúl. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valetti, Julian Alonso. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Salas, Nancy Edith. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martino, Adolfo Ludovico. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMagnolia Press2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24055Otero, Manuel Alejandro; Grenat, Pablo Raúl; Valetti, Julian Alonso; Salas, Nancy Edith; Martino, Adolfo Ludovico; Erythrocyte nuclear size as a better diagnostic character than cell size in the identification of live cryptic polyploid species; Magnolia Press; Zootaxa; 3694; 3; 8-2013; 262-2701175-53261175-5334CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.11646/zootaxa.3694.3.6info:eu-repo/semantics/altIdentifier/url/https://biotaxa.org/Zootaxa/article/view/zootaxa.3694.3.6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:32:54Zoai:ri.conicet.gov.ar:11336/24055instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:32:54.657CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Erythrocyte nuclear size as a better diagnostic character than cell size in the identification of live cryptic polyploid species
title Erythrocyte nuclear size as a better diagnostic character than cell size in the identification of live cryptic polyploid species
spellingShingle Erythrocyte nuclear size as a better diagnostic character than cell size in the identification of live cryptic polyploid species
Otero, Manuel Alejandro
Erythrocyte
Nuclear Size
Cryptic Polyploid Species
title_short Erythrocyte nuclear size as a better diagnostic character than cell size in the identification of live cryptic polyploid species
title_full Erythrocyte nuclear size as a better diagnostic character than cell size in the identification of live cryptic polyploid species
title_fullStr Erythrocyte nuclear size as a better diagnostic character than cell size in the identification of live cryptic polyploid species
title_full_unstemmed Erythrocyte nuclear size as a better diagnostic character than cell size in the identification of live cryptic polyploid species
title_sort Erythrocyte nuclear size as a better diagnostic character than cell size in the identification of live cryptic polyploid species
dc.creator.none.fl_str_mv Otero, Manuel Alejandro
Grenat, Pablo Raúl
Valetti, Julian Alonso
Salas, Nancy Edith
Martino, Adolfo Ludovico
author Otero, Manuel Alejandro
author_facet Otero, Manuel Alejandro
Grenat, Pablo Raúl
Valetti, Julian Alonso
Salas, Nancy Edith
Martino, Adolfo Ludovico
author_role author
author2 Grenat, Pablo Raúl
Valetti, Julian Alonso
Salas, Nancy Edith
Martino, Adolfo Ludovico
author2_role author
author
author
author
dc.subject.none.fl_str_mv Erythrocyte
Nuclear Size
Cryptic Polyploid Species
topic Erythrocyte
Nuclear Size
Cryptic Polyploid Species
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv It is well documented in anurans the cryptic condition of many species complexes involving polyploids. In these complexes the character that clearly differentiates them is the number of chromosome complements. The blood cells of amphibians conserve their nucleus, and so the erythrocyte size is correlated with the DNA content. We analyzed two cryptic-polyploid complexes occurring in the center of Argentina: Odontophrynus cordobae (2n)/O. americanus (4n) and Pleurodema kriegi (4n)/P. cordobae (8n). Our aim was evaluate the efficiency in the utilization of nuclear area with respect to cellular area of the erythrocytes to define the limits values for the identification of cryptic-polyploid species. We studied 110 individuals of Pleurodema and 116 individuals of Odontoprhynus. For each individual, we measured the cellular and nuclear length (L) and width (A) of 40 erythrocytes (Area= L*A*π/4) and boundary values were calculated using distribution curves. In both complexes studied, the erythrometric parameters showed significant differences between related species. Moreover, in both complexes the nuclear area was more efficient for identifying the species (Pleurodema: 34.39 μm² (probability=99.96%) and Odontophrynus: 24.02 μm² (99.075%)) than the cell area (Pleurodema: 273.08 μm² (97.55%) y Odontophrynus: 197.69 μm² (97.94%)). Greater efficiency found using nuclear area is novel and significant because most studies use only the cell area to differentiate polyploid complexes.
Fil: Otero, Manuel Alejandro. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grenat, Pablo Raúl. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valetti, Julian Alonso. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salas, Nancy Edith. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martino, Adolfo Ludovico. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description It is well documented in anurans the cryptic condition of many species complexes involving polyploids. In these complexes the character that clearly differentiates them is the number of chromosome complements. The blood cells of amphibians conserve their nucleus, and so the erythrocyte size is correlated with the DNA content. We analyzed two cryptic-polyploid complexes occurring in the center of Argentina: Odontophrynus cordobae (2n)/O. americanus (4n) and Pleurodema kriegi (4n)/P. cordobae (8n). Our aim was evaluate the efficiency in the utilization of nuclear area with respect to cellular area of the erythrocytes to define the limits values for the identification of cryptic-polyploid species. We studied 110 individuals of Pleurodema and 116 individuals of Odontoprhynus. For each individual, we measured the cellular and nuclear length (L) and width (A) of 40 erythrocytes (Area= L*A*π/4) and boundary values were calculated using distribution curves. In both complexes studied, the erythrometric parameters showed significant differences between related species. Moreover, in both complexes the nuclear area was more efficient for identifying the species (Pleurodema: 34.39 μm² (probability=99.96%) and Odontophrynus: 24.02 μm² (99.075%)) than the cell area (Pleurodema: 273.08 μm² (97.55%) y Odontophrynus: 197.69 μm² (97.94%)). Greater efficiency found using nuclear area is novel and significant because most studies use only the cell area to differentiate polyploid complexes.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24055
Otero, Manuel Alejandro; Grenat, Pablo Raúl; Valetti, Julian Alonso; Salas, Nancy Edith; Martino, Adolfo Ludovico; Erythrocyte nuclear size as a better diagnostic character than cell size in the identification of live cryptic polyploid species; Magnolia Press; Zootaxa; 3694; 3; 8-2013; 262-270
1175-5326
1175-5334
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24055
identifier_str_mv Otero, Manuel Alejandro; Grenat, Pablo Raúl; Valetti, Julian Alonso; Salas, Nancy Edith; Martino, Adolfo Ludovico; Erythrocyte nuclear size as a better diagnostic character than cell size in the identification of live cryptic polyploid species; Magnolia Press; Zootaxa; 3694; 3; 8-2013; 262-270
1175-5326
1175-5334
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.11646/zootaxa.3694.3.6
info:eu-repo/semantics/altIdentifier/url/https://biotaxa.org/Zootaxa/article/view/zootaxa.3694.3.6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Magnolia Press
publisher.none.fl_str_mv Magnolia Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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