Outdoor culture of Halamphora coffeaeformis in the semi-arid Pampa of Argentina: A comprehensive analysis of triacylglycerol production for biodiesel

Autores
Navarro, Francisco Ezequiel; Leonardi, Patricia Ines; Damiani, Maria Cecilia; Martin, Lucas Ariel; Martínez, A. M.; Popovich, Cecilia Angelines
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The feasibility of microalgal triacylglycerol (TAG) production for biodiesel remains constrained by high production costs under outdoor conditions. The aim of this study was to evaluate the capacity of an Argentinian strain of the diatom Halamphora coffeaeformis grown in outdoor raceway ponds to produce TAG suitable for biodiesel. The strain was cultured in seawater enriched with a biofertilizer during summer and spring in the semi-arid Pampa of Argentina. The following variables were analysed: 1) growth rate; 2) TAG accumulation; 3) lipid content and quality; 4) dissolved nutrient and physicochemical properties; and 5) climatic conditions. Intracellular TAG accumulation was evaluated with fluorometry by Nile Red. The growth rate and biomass productivity of the species were similar in the two seasons, with approximate values of 1.35 div. day−1 and 61.25 kg.ha−1.day−1, respectively. Total lipid content increased about 4-fold with respect to inocula in the 2 cultures, reaching up to 44.7 % ash free dry weight (AFDW) in summer and 36.4 % AFDW in spring. However, lipid accumulation differed between seasons: neutral lipid accumulation began at 8 days of cultivation in summer and at 19 days in spring. Salinity was the main stressor that accelerated the TAG accumulation in the species, the nutrient levels did not reach limiting values. In spring, precipitation decreased salinity levels, reducing stress and delaying the onset of TAG accumulation. The biodiesel properties inferred from the fatty acid profile of the species meet international standards and their quality ensures good performance in cold climates. Furthermore, the reported lipid productivity of the species (~25.6 kg.ha−1.day−1) is higher than that of the soybean oil used commercially for biodiesel. Based on the results obtained, H. coffeaeformis has potential for exploitation as a renewable source of TAG for biodiesel production in arid and semi-arid environments with access to brackish or marine water.
Fil: Navarro, Francisco Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Damiani, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Martin, Lucas Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Martínez, A. M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Provincia de Buenos Aires. Dirección General de Cultura y Educación. Universidad Provincial del Sudoeste. Centro de Emprendedorismo y Desarrollo Territorial Sustentable; Argentina
Materia
BIODIESEL
BIOENERGY CULTURE
DIATOMS
RACEWAY POND
SEAWATER
Nivel de accesibilidad
acceso embargado
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/216063
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Outdoor culture of Halamphora coffeaeformis in the semi-arid Pampa of Argentina: A comprehensive analysis of triacylglycerol production for biodieselNavarro, Francisco EzequielLeonardi, Patricia InesDamiani, Maria CeciliaMartin, Lucas ArielMartínez, A. M.Popovich, Cecilia AngelinesBIODIESELBIOENERGY CULTUREDIATOMSRACEWAY PONDSEAWATERhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The feasibility of microalgal triacylglycerol (TAG) production for biodiesel remains constrained by high production costs under outdoor conditions. The aim of this study was to evaluate the capacity of an Argentinian strain of the diatom Halamphora coffeaeformis grown in outdoor raceway ponds to produce TAG suitable for biodiesel. The strain was cultured in seawater enriched with a biofertilizer during summer and spring in the semi-arid Pampa of Argentina. The following variables were analysed: 1) growth rate; 2) TAG accumulation; 3) lipid content and quality; 4) dissolved nutrient and physicochemical properties; and 5) climatic conditions. Intracellular TAG accumulation was evaluated with fluorometry by Nile Red. The growth rate and biomass productivity of the species were similar in the two seasons, with approximate values of 1.35 div. day−1 and 61.25 kg.ha−1.day−1, respectively. Total lipid content increased about 4-fold with respect to inocula in the 2 cultures, reaching up to 44.7 % ash free dry weight (AFDW) in summer and 36.4 % AFDW in spring. However, lipid accumulation differed between seasons: neutral lipid accumulation began at 8 days of cultivation in summer and at 19 days in spring. Salinity was the main stressor that accelerated the TAG accumulation in the species, the nutrient levels did not reach limiting values. In spring, precipitation decreased salinity levels, reducing stress and delaying the onset of TAG accumulation. The biodiesel properties inferred from the fatty acid profile of the species meet international standards and their quality ensures good performance in cold climates. Furthermore, the reported lipid productivity of the species (~25.6 kg.ha−1.day−1) is higher than that of the soybean oil used commercially for biodiesel. Based on the results obtained, H. coffeaeformis has potential for exploitation as a renewable source of TAG for biodiesel production in arid and semi-arid environments with access to brackish or marine water.Fil: Navarro, Francisco Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Damiani, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Martin, Lucas Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Martínez, A. M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Provincia de Buenos Aires. Dirección General de Cultura y Educación. Universidad Provincial del Sudoeste. Centro de Emprendedorismo y Desarrollo Territorial Sustentable; ArgentinaElsevier2023-07info:eu-repo/date/embargoEnd/2024-04-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/216063Navarro, Francisco Ezequiel; Leonardi, Patricia Ines; Damiani, Maria Cecilia; Martin, Lucas Ariel; Martínez, A. M.; et al.; Outdoor culture of Halamphora coffeaeformis in the semi-arid Pampa of Argentina: A comprehensive analysis of triacylglycerol production for biodiesel; Elsevier; Algal Research; 74; 7-2023; 1-432211-9264CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211926423002035info:eu-repo/semantics/altIdentifier/doi/10.1016/j.algal.2023.103170info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:11:10Zoai:ri.conicet.gov.ar:11336/216063instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:11:11.237CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Outdoor culture of Halamphora coffeaeformis in the semi-arid Pampa of Argentina: A comprehensive analysis of triacylglycerol production for biodiesel
title Outdoor culture of Halamphora coffeaeformis in the semi-arid Pampa of Argentina: A comprehensive analysis of triacylglycerol production for biodiesel
spellingShingle Outdoor culture of Halamphora coffeaeformis in the semi-arid Pampa of Argentina: A comprehensive analysis of triacylglycerol production for biodiesel
Navarro, Francisco Ezequiel
BIODIESEL
BIOENERGY CULTURE
DIATOMS
RACEWAY POND
SEAWATER
title_short Outdoor culture of Halamphora coffeaeformis in the semi-arid Pampa of Argentina: A comprehensive analysis of triacylglycerol production for biodiesel
title_full Outdoor culture of Halamphora coffeaeformis in the semi-arid Pampa of Argentina: A comprehensive analysis of triacylglycerol production for biodiesel
title_fullStr Outdoor culture of Halamphora coffeaeformis in the semi-arid Pampa of Argentina: A comprehensive analysis of triacylglycerol production for biodiesel
title_full_unstemmed Outdoor culture of Halamphora coffeaeformis in the semi-arid Pampa of Argentina: A comprehensive analysis of triacylglycerol production for biodiesel
title_sort Outdoor culture of Halamphora coffeaeformis in the semi-arid Pampa of Argentina: A comprehensive analysis of triacylglycerol production for biodiesel
dc.creator.none.fl_str_mv Navarro, Francisco Ezequiel
Leonardi, Patricia Ines
Damiani, Maria Cecilia
Martin, Lucas Ariel
Martínez, A. M.
Popovich, Cecilia Angelines
author Navarro, Francisco Ezequiel
author_facet Navarro, Francisco Ezequiel
Leonardi, Patricia Ines
Damiani, Maria Cecilia
Martin, Lucas Ariel
Martínez, A. M.
Popovich, Cecilia Angelines
author_role author
author2 Leonardi, Patricia Ines
Damiani, Maria Cecilia
Martin, Lucas Ariel
Martínez, A. M.
Popovich, Cecilia Angelines
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIODIESEL
BIOENERGY CULTURE
DIATOMS
RACEWAY POND
SEAWATER
topic BIODIESEL
BIOENERGY CULTURE
DIATOMS
RACEWAY POND
SEAWATER
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The feasibility of microalgal triacylglycerol (TAG) production for biodiesel remains constrained by high production costs under outdoor conditions. The aim of this study was to evaluate the capacity of an Argentinian strain of the diatom Halamphora coffeaeformis grown in outdoor raceway ponds to produce TAG suitable for biodiesel. The strain was cultured in seawater enriched with a biofertilizer during summer and spring in the semi-arid Pampa of Argentina. The following variables were analysed: 1) growth rate; 2) TAG accumulation; 3) lipid content and quality; 4) dissolved nutrient and physicochemical properties; and 5) climatic conditions. Intracellular TAG accumulation was evaluated with fluorometry by Nile Red. The growth rate and biomass productivity of the species were similar in the two seasons, with approximate values of 1.35 div. day−1 and 61.25 kg.ha−1.day−1, respectively. Total lipid content increased about 4-fold with respect to inocula in the 2 cultures, reaching up to 44.7 % ash free dry weight (AFDW) in summer and 36.4 % AFDW in spring. However, lipid accumulation differed between seasons: neutral lipid accumulation began at 8 days of cultivation in summer and at 19 days in spring. Salinity was the main stressor that accelerated the TAG accumulation in the species, the nutrient levels did not reach limiting values. In spring, precipitation decreased salinity levels, reducing stress and delaying the onset of TAG accumulation. The biodiesel properties inferred from the fatty acid profile of the species meet international standards and their quality ensures good performance in cold climates. Furthermore, the reported lipid productivity of the species (~25.6 kg.ha−1.day−1) is higher than that of the soybean oil used commercially for biodiesel. Based on the results obtained, H. coffeaeformis has potential for exploitation as a renewable source of TAG for biodiesel production in arid and semi-arid environments with access to brackish or marine water.
Fil: Navarro, Francisco Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Damiani, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Martin, Lucas Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Martínez, A. M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Provincia de Buenos Aires. Dirección General de Cultura y Educación. Universidad Provincial del Sudoeste. Centro de Emprendedorismo y Desarrollo Territorial Sustentable; Argentina
description The feasibility of microalgal triacylglycerol (TAG) production for biodiesel remains constrained by high production costs under outdoor conditions. The aim of this study was to evaluate the capacity of an Argentinian strain of the diatom Halamphora coffeaeformis grown in outdoor raceway ponds to produce TAG suitable for biodiesel. The strain was cultured in seawater enriched with a biofertilizer during summer and spring in the semi-arid Pampa of Argentina. The following variables were analysed: 1) growth rate; 2) TAG accumulation; 3) lipid content and quality; 4) dissolved nutrient and physicochemical properties; and 5) climatic conditions. Intracellular TAG accumulation was evaluated with fluorometry by Nile Red. The growth rate and biomass productivity of the species were similar in the two seasons, with approximate values of 1.35 div. day−1 and 61.25 kg.ha−1.day−1, respectively. Total lipid content increased about 4-fold with respect to inocula in the 2 cultures, reaching up to 44.7 % ash free dry weight (AFDW) in summer and 36.4 % AFDW in spring. However, lipid accumulation differed between seasons: neutral lipid accumulation began at 8 days of cultivation in summer and at 19 days in spring. Salinity was the main stressor that accelerated the TAG accumulation in the species, the nutrient levels did not reach limiting values. In spring, precipitation decreased salinity levels, reducing stress and delaying the onset of TAG accumulation. The biodiesel properties inferred from the fatty acid profile of the species meet international standards and their quality ensures good performance in cold climates. Furthermore, the reported lipid productivity of the species (~25.6 kg.ha−1.day−1) is higher than that of the soybean oil used commercially for biodiesel. Based on the results obtained, H. coffeaeformis has potential for exploitation as a renewable source of TAG for biodiesel production in arid and semi-arid environments with access to brackish or marine water.
publishDate 2023
dc.date.none.fl_str_mv 2023-07
info:eu-repo/date/embargoEnd/2024-04-26
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/216063
Navarro, Francisco Ezequiel; Leonardi, Patricia Ines; Damiani, Maria Cecilia; Martin, Lucas Ariel; Martínez, A. M.; et al.; Outdoor culture of Halamphora coffeaeformis in the semi-arid Pampa of Argentina: A comprehensive analysis of triacylglycerol production for biodiesel; Elsevier; Algal Research; 74; 7-2023; 1-43
2211-9264
CONICET Digital
CONICET
url http://hdl.handle.net/11336/216063
identifier_str_mv Navarro, Francisco Ezequiel; Leonardi, Patricia Ines; Damiani, Maria Cecilia; Martin, Lucas Ariel; Martínez, A. M.; et al.; Outdoor culture of Halamphora coffeaeformis in the semi-arid Pampa of Argentina: A comprehensive analysis of triacylglycerol production for biodiesel; Elsevier; Algal Research; 74; 7-2023; 1-43
2211-9264
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211926423002035
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.algal.2023.103170
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
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eu_rights_str_mv embargoedAccess
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dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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