New amphiphilic amino acid derivatives for efficient DNA transfection in vitro

Autores
Peña, Lucía Carolina; Argarañá, María Fernanda; de Zan, María Mercedes; Giorello, Antonella; Antuña, Sebastián; Prieto, Claudio; Veaute, Carolina Melania Isabel; Muller, Diana
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nucleic acids-based therapies have recently developed as next-generationagents for treating and preventing viral infection, cancer, and genetic disorders,but their use is still limited due to its relatively poor delivery into targetedcells. We designed and synthesized new amphiphilic amino acid derivatives(cysteine-based) of low molecular weight, formed by the same pentapeptide(AG2: WWCOO) N-acylated, with different hydrophobic chains containingfrom 12 to 18 carbons, named AG2-Cn (N), which dimerize by oxidationin the presence of pLenti-CMV-GFP Puro plasmid (P) in the respectivegemini. We determined transfection efficiency, critical micelle concentration,particle size, ζ-potential and cytotoxicity for the derivatives obtained. Wefound that all the synthesized compounds were active for DNA delivery andhad greater ability to transfect CHO-K1 cells. In particular, AG2-C18 is apromising carrier for gene delivery because it showed no cytotoxicity and itsactivity was greater than or equal to the commercial actives currently used.
Fil: Peña, Lucía Carolina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Argarañá, María Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: de Zan, María Mercedes. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Giorello, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica "Ing. José Miguel Parera". Universidad Nacional del Litoral. Instituto de Investigaciones en Catálisis y Petroquímica "Ing. José Miguel Parera"; Argentina
Fil: Antuña, Sebastián. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Prieto, Claudio. Universidad Nacional del Litoral; Argentina
Fil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Muller, Diana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Materia
AMPHIPHILE
CYSTEINE
AMPHIPHILE
CYSTEINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/63493
Acceder
id CONICETDig_493d4c44f6d344aff0f58ccd3434e885
oai_identifier_str oai:ri.conicet.gov.ar:11336/63493
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling New amphiphilic amino acid derivatives for efficient DNA transfection in vitroPeña, Lucía CarolinaArgarañá, María Fernandade Zan, María MercedesGiorello, AntonellaAntuña, SebastiánPrieto, ClaudioVeaute, Carolina Melania IsabelMuller, DianaAMPHIPHILECYSTEINEAMPHIPHILECYSTEINEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Nucleic acids-based therapies have recently developed as next-generationagents for treating and preventing viral infection, cancer, and genetic disorders,but their use is still limited due to its relatively poor delivery into targetedcells. We designed and synthesized new amphiphilic amino acid derivatives(cysteine-based) of low molecular weight, formed by the same pentapeptide(AG2: WWCOO) N-acylated, with different hydrophobic chains containingfrom 12 to 18 carbons, named AG2-Cn (N), which dimerize by oxidationin the presence of pLenti-CMV-GFP Puro plasmid (P) in the respectivegemini. We determined transfection efficiency, critical micelle concentration,particle size, ζ-potential and cytotoxicity for the derivatives obtained. Wefound that all the synthesized compounds were active for DNA delivery andhad greater ability to transfect CHO-K1 cells. In particular, AG2-C18 is apromising carrier for gene delivery because it showed no cytotoxicity and itsactivity was greater than or equal to the commercial actives currently used.Fil: Peña, Lucía Carolina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Argarañá, María Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: de Zan, María Mercedes. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Giorello, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica "Ing. José Miguel Parera". Universidad Nacional del Litoral. Instituto de Investigaciones en Catálisis y Petroquímica "Ing. José Miguel Parera"; ArgentinaFil: Antuña, Sebastián. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Prieto, Claudio. Universidad Nacional del Litoral; ArgentinaFil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Muller, Diana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaScientific Research Publishing2017-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/63493Peña, Lucía Carolina; Argarañá, María Fernanda; de Zan, María Mercedes; Giorello, Antonella; Antuña, Sebastián; et al.; New amphiphilic amino acid derivatives for efficient DNA transfection in vitro; Scientific Research Publishing; Advances in Chemical Engineering and Science; 07; 02; 4-2017; 191-2052160-0406CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/acesinfo:eu-repo/semantics/altIdentifier/doi/10.4236/aces.2017.72014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:18Zoai:ri.conicet.gov.ar:11336/63493instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:19.024CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv New amphiphilic amino acid derivatives for efficient DNA transfection in vitro
title New amphiphilic amino acid derivatives for efficient DNA transfection in vitro
spellingShingle New amphiphilic amino acid derivatives for efficient DNA transfection in vitro
Peña, Lucía Carolina
AMPHIPHILE
CYSTEINE
AMPHIPHILE
CYSTEINE
title_short New amphiphilic amino acid derivatives for efficient DNA transfection in vitro
title_full New amphiphilic amino acid derivatives for efficient DNA transfection in vitro
title_fullStr New amphiphilic amino acid derivatives for efficient DNA transfection in vitro
title_full_unstemmed New amphiphilic amino acid derivatives for efficient DNA transfection in vitro
title_sort New amphiphilic amino acid derivatives for efficient DNA transfection in vitro
dc.creator.none.fl_str_mv Peña, Lucía Carolina
Argarañá, María Fernanda
de Zan, María Mercedes
Giorello, Antonella
Antuña, Sebastián
Prieto, Claudio
Veaute, Carolina Melania Isabel
Muller, Diana
author Peña, Lucía Carolina
author_facet Peña, Lucía Carolina
Argarañá, María Fernanda
de Zan, María Mercedes
Giorello, Antonella
Antuña, Sebastián
Prieto, Claudio
Veaute, Carolina Melania Isabel
Muller, Diana
author_role author
author2 Argarañá, María Fernanda
de Zan, María Mercedes
Giorello, Antonella
Antuña, Sebastián
Prieto, Claudio
Veaute, Carolina Melania Isabel
Muller, Diana
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AMPHIPHILE
CYSTEINE
AMPHIPHILE
CYSTEINE
topic AMPHIPHILE
CYSTEINE
AMPHIPHILE
CYSTEINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nucleic acids-based therapies have recently developed as next-generationagents for treating and preventing viral infection, cancer, and genetic disorders,but their use is still limited due to its relatively poor delivery into targetedcells. We designed and synthesized new amphiphilic amino acid derivatives(cysteine-based) of low molecular weight, formed by the same pentapeptide(AG2: WWCOO) N-acylated, with different hydrophobic chains containingfrom 12 to 18 carbons, named AG2-Cn (N), which dimerize by oxidationin the presence of pLenti-CMV-GFP Puro plasmid (P) in the respectivegemini. We determined transfection efficiency, critical micelle concentration,particle size, ζ-potential and cytotoxicity for the derivatives obtained. Wefound that all the synthesized compounds were active for DNA delivery andhad greater ability to transfect CHO-K1 cells. In particular, AG2-C18 is apromising carrier for gene delivery because it showed no cytotoxicity and itsactivity was greater than or equal to the commercial actives currently used.
Fil: Peña, Lucía Carolina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Argarañá, María Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: de Zan, María Mercedes. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Giorello, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica "Ing. José Miguel Parera". Universidad Nacional del Litoral. Instituto de Investigaciones en Catálisis y Petroquímica "Ing. José Miguel Parera"; Argentina
Fil: Antuña, Sebastián. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Prieto, Claudio. Universidad Nacional del Litoral; Argentina
Fil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Muller, Diana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
description Nucleic acids-based therapies have recently developed as next-generationagents for treating and preventing viral infection, cancer, and genetic disorders,but their use is still limited due to its relatively poor delivery into targetedcells. We designed and synthesized new amphiphilic amino acid derivatives(cysteine-based) of low molecular weight, formed by the same pentapeptide(AG2: WWCOO) N-acylated, with different hydrophobic chains containingfrom 12 to 18 carbons, named AG2-Cn (N), which dimerize by oxidationin the presence of pLenti-CMV-GFP Puro plasmid (P) in the respectivegemini. We determined transfection efficiency, critical micelle concentration,particle size, ζ-potential and cytotoxicity for the derivatives obtained. Wefound that all the synthesized compounds were active for DNA delivery andhad greater ability to transfect CHO-K1 cells. In particular, AG2-C18 is apromising carrier for gene delivery because it showed no cytotoxicity and itsactivity was greater than or equal to the commercial actives currently used.
publishDate 2017
dc.date.none.fl_str_mv 2017-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/63493
Peña, Lucía Carolina; Argarañá, María Fernanda; de Zan, María Mercedes; Giorello, Antonella; Antuña, Sebastián; et al.; New amphiphilic amino acid derivatives for efficient DNA transfection in vitro; Scientific Research Publishing; Advances in Chemical Engineering and Science; 07; 02; 4-2017; 191-205
2160-0406
CONICET Digital
CONICET
url http://hdl.handle.net/11336/63493
identifier_str_mv Peña, Lucía Carolina; Argarañá, María Fernanda; de Zan, María Mercedes; Giorello, Antonella; Antuña, Sebastián; et al.; New amphiphilic amino acid derivatives for efficient DNA transfection in vitro; Scientific Research Publishing; Advances in Chemical Engineering and Science; 07; 02; 4-2017; 191-205
2160-0406
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/aces
info:eu-repo/semantics/altIdentifier/doi/10.4236/aces.2017.72014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific Research Publishing
publisher.none.fl_str_mv Scientific Research Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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