Molecular epidemiology of foot-and-mouth disease virus type A in South America

Autores
Malirat, Viviana; Bergmann, Ingrid Evelyn; Campos, Renata de Mendonça; Conde, Florangel; Quiroga, José Luis; Villamil, Mariluz; Salgado, Gustavo; Ortiz, Salomón
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A databank of 78 VP1 complete sequences of type A foot-and-mouth disease virus (FMDV) from South American isolates was constructed. Forty-nine samples corresponded to FMDV that circulated between the years 1999–2008, mainly in Venezuela, where most type A outbreaks have occurred lately and twenty-nine to strains historically relevant for the continent. The phylogenetic analysis showed that all South American FMDV belonged to the Euro-SA topotype. Sixteen subgenotypes could be identified, based on a 15% nucleotide divergence cut-off criterion: eight are extinguished, three were active until the year 2002 and the remaining five circulated in Venezuela during the years 2001–2007, illustrating the potential for FMDV diversification under appropriate selective pressure. The last emergencies reported in already-free areas of Colombia in 2004 and 2008 were closely related to isolates acting in Venzuela. Evidence of positive selection over codon 170, within the immunogenic site 4 of VP1 protein, was recorded. A codon deletion in amino acid position 142, within the G–H loop, was found in some isolates within subgenotypes 14, 15 and 16. Conversely amino acid deletion 197 was restricted to all isolates within a particular genetic cluster. The present work is the first comprehensive phylogenetic analysis of FMDV type A in South America, filling a gap of knowledge with respect to both, historical and acting viruses. The results provided evidence that supports the ecosystem dynamics in the region, and also served as an input to establish genetic links of emergencies in already-declared free areas, highlighting the need for strengthening control activities.
Fil: Malirat, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. Cesar Milstein". Fundación Pablo Cassara. Instituto de Ciencia y Tecnología ; Argentina. Pan American Foot-and-Mouth Disease Center; Brasil
Fil: Bergmann, Ingrid Evelyn. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. Cesar Milstein". Fundación Pablo Cassara. Instituto de Ciencia y Tecnología ; Argentina. Pan American Foot-and-Mouth Disease Center; Brasil
Fil: Campos, Renata de Mendonça. Pan American Foot-and-Mouth Disease Center; Brasil. Instituto Oswaldo Cruz; Brasil
Fil: Conde, Florangel. Centro Nacional de Investigaciones Agropecuarias. Instituto Nacional de Investigaciones Agrícolas; Venezuela
Fil: Quiroga, José Luis. Laboratorio de Investigación y Diagnóstico Veterinario; Bolivia
Fil: Villamil, Mariluz. Laboratorio Nacional de Diagnóstico Veterinario; Colombia
Fil: Salgado, Gustavo. Instituto de Medicina e Higiene Tropical ‘‘Izquieta Perez’; Ecuador
Fil: Ortiz, Salomón. Servicio Nacional de Sanidad Animal; Perú
Materia
Foot-And-Mouth Disease Type a Virus
Molecular Epidemiology
Sequence Analysis
Europesouth America Fmdv Topotype
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16895
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/16895
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular epidemiology of foot-and-mouth disease virus type A in South AmericaMalirat, VivianaBergmann, Ingrid EvelynCampos, Renata de MendonçaConde, FlorangelQuiroga, José LuisVillamil, MariluzSalgado, GustavoOrtiz, SalomónFoot-And-Mouth Disease Type a VirusMolecular EpidemiologySequence AnalysisEuropesouth America Fmdv Topotypehttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4A databank of 78 VP1 complete sequences of type A foot-and-mouth disease virus (FMDV) from South American isolates was constructed. Forty-nine samples corresponded to FMDV that circulated between the years 1999–2008, mainly in Venezuela, where most type A outbreaks have occurred lately and twenty-nine to strains historically relevant for the continent. The phylogenetic analysis showed that all South American FMDV belonged to the Euro-SA topotype. Sixteen subgenotypes could be identified, based on a 15% nucleotide divergence cut-off criterion: eight are extinguished, three were active until the year 2002 and the remaining five circulated in Venezuela during the years 2001–2007, illustrating the potential for FMDV diversification under appropriate selective pressure. The last emergencies reported in already-free areas of Colombia in 2004 and 2008 were closely related to isolates acting in Venzuela. Evidence of positive selection over codon 170, within the immunogenic site 4 of VP1 protein, was recorded. A codon deletion in amino acid position 142, within the G–H loop, was found in some isolates within subgenotypes 14, 15 and 16. Conversely amino acid deletion 197 was restricted to all isolates within a particular genetic cluster. The present work is the first comprehensive phylogenetic analysis of FMDV type A in South America, filling a gap of knowledge with respect to both, historical and acting viruses. The results provided evidence that supports the ecosystem dynamics in the region, and also served as an input to establish genetic links of emergencies in already-declared free areas, highlighting the need for strengthening control activities.Fil: Malirat, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. Cesar Milstein". Fundación Pablo Cassara. Instituto de Ciencia y Tecnología ; Argentina. Pan American Foot-and-Mouth Disease Center; BrasilFil: Bergmann, Ingrid Evelyn. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. Cesar Milstein". Fundación Pablo Cassara. Instituto de Ciencia y Tecnología ; Argentina. Pan American Foot-and-Mouth Disease Center; BrasilFil: Campos, Renata de Mendonça. Pan American Foot-and-Mouth Disease Center; Brasil. Instituto Oswaldo Cruz; BrasilFil: Conde, Florangel. Centro Nacional de Investigaciones Agropecuarias. Instituto Nacional de Investigaciones Agrícolas; VenezuelaFil: Quiroga, José Luis. Laboratorio de Investigación y Diagnóstico Veterinario; BoliviaFil: Villamil, Mariluz. Laboratorio Nacional de Diagnóstico Veterinario; ColombiaFil: Salgado, Gustavo. Instituto de Medicina e Higiene Tropical ‘‘Izquieta Perez’; EcuadorFil: Ortiz, Salomón. Servicio Nacional de Sanidad Animal; PerúElsevier Science2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16895Malirat, Viviana; Bergmann, Ingrid Evelyn; Campos, Renata de Mendonça; Conde, Florangel; Quiroga, José Luis; et al.; Molecular epidemiology of foot-and-mouth disease virus type A in South America; Elsevier Science; Veterinary Microbiology; 158; 1-2; 7-2012; 82-940378-1135enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetmic.2012.02.009info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0378113512001198info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:13:48Zoai:ri.conicet.gov.ar:11336/16895instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:13:49.182CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular epidemiology of foot-and-mouth disease virus type A in South America
title Molecular epidemiology of foot-and-mouth disease virus type A in South America
spellingShingle Molecular epidemiology of foot-and-mouth disease virus type A in South America
Malirat, Viviana
Foot-And-Mouth Disease Type a Virus
Molecular Epidemiology
Sequence Analysis
Europesouth America Fmdv Topotype
title_short Molecular epidemiology of foot-and-mouth disease virus type A in South America
title_full Molecular epidemiology of foot-and-mouth disease virus type A in South America
title_fullStr Molecular epidemiology of foot-and-mouth disease virus type A in South America
title_full_unstemmed Molecular epidemiology of foot-and-mouth disease virus type A in South America
title_sort Molecular epidemiology of foot-and-mouth disease virus type A in South America
dc.creator.none.fl_str_mv Malirat, Viviana
Bergmann, Ingrid Evelyn
Campos, Renata de Mendonça
Conde, Florangel
Quiroga, José Luis
Villamil, Mariluz
Salgado, Gustavo
Ortiz, Salomón
author Malirat, Viviana
author_facet Malirat, Viviana
Bergmann, Ingrid Evelyn
Campos, Renata de Mendonça
Conde, Florangel
Quiroga, José Luis
Villamil, Mariluz
Salgado, Gustavo
Ortiz, Salomón
author_role author
author2 Bergmann, Ingrid Evelyn
Campos, Renata de Mendonça
Conde, Florangel
Quiroga, José Luis
Villamil, Mariluz
Salgado, Gustavo
Ortiz, Salomón
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Foot-And-Mouth Disease Type a Virus
Molecular Epidemiology
Sequence Analysis
Europesouth America Fmdv Topotype
topic Foot-And-Mouth Disease Type a Virus
Molecular Epidemiology
Sequence Analysis
Europesouth America Fmdv Topotype
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv A databank of 78 VP1 complete sequences of type A foot-and-mouth disease virus (FMDV) from South American isolates was constructed. Forty-nine samples corresponded to FMDV that circulated between the years 1999–2008, mainly in Venezuela, where most type A outbreaks have occurred lately and twenty-nine to strains historically relevant for the continent. The phylogenetic analysis showed that all South American FMDV belonged to the Euro-SA topotype. Sixteen subgenotypes could be identified, based on a 15% nucleotide divergence cut-off criterion: eight are extinguished, three were active until the year 2002 and the remaining five circulated in Venezuela during the years 2001–2007, illustrating the potential for FMDV diversification under appropriate selective pressure. The last emergencies reported in already-free areas of Colombia in 2004 and 2008 were closely related to isolates acting in Venzuela. Evidence of positive selection over codon 170, within the immunogenic site 4 of VP1 protein, was recorded. A codon deletion in amino acid position 142, within the G–H loop, was found in some isolates within subgenotypes 14, 15 and 16. Conversely amino acid deletion 197 was restricted to all isolates within a particular genetic cluster. The present work is the first comprehensive phylogenetic analysis of FMDV type A in South America, filling a gap of knowledge with respect to both, historical and acting viruses. The results provided evidence that supports the ecosystem dynamics in the region, and also served as an input to establish genetic links of emergencies in already-declared free areas, highlighting the need for strengthening control activities.
Fil: Malirat, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. Cesar Milstein". Fundación Pablo Cassara. Instituto de Ciencia y Tecnología ; Argentina. Pan American Foot-and-Mouth Disease Center; Brasil
Fil: Bergmann, Ingrid Evelyn. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. Cesar Milstein". Fundación Pablo Cassara. Instituto de Ciencia y Tecnología ; Argentina. Pan American Foot-and-Mouth Disease Center; Brasil
Fil: Campos, Renata de Mendonça. Pan American Foot-and-Mouth Disease Center; Brasil. Instituto Oswaldo Cruz; Brasil
Fil: Conde, Florangel. Centro Nacional de Investigaciones Agropecuarias. Instituto Nacional de Investigaciones Agrícolas; Venezuela
Fil: Quiroga, José Luis. Laboratorio de Investigación y Diagnóstico Veterinario; Bolivia
Fil: Villamil, Mariluz. Laboratorio Nacional de Diagnóstico Veterinario; Colombia
Fil: Salgado, Gustavo. Instituto de Medicina e Higiene Tropical ‘‘Izquieta Perez’; Ecuador
Fil: Ortiz, Salomón. Servicio Nacional de Sanidad Animal; Perú
description A databank of 78 VP1 complete sequences of type A foot-and-mouth disease virus (FMDV) from South American isolates was constructed. Forty-nine samples corresponded to FMDV that circulated between the years 1999–2008, mainly in Venezuela, where most type A outbreaks have occurred lately and twenty-nine to strains historically relevant for the continent. The phylogenetic analysis showed that all South American FMDV belonged to the Euro-SA topotype. Sixteen subgenotypes could be identified, based on a 15% nucleotide divergence cut-off criterion: eight are extinguished, three were active until the year 2002 and the remaining five circulated in Venezuela during the years 2001–2007, illustrating the potential for FMDV diversification under appropriate selective pressure. The last emergencies reported in already-free areas of Colombia in 2004 and 2008 were closely related to isolates acting in Venzuela. Evidence of positive selection over codon 170, within the immunogenic site 4 of VP1 protein, was recorded. A codon deletion in amino acid position 142, within the G–H loop, was found in some isolates within subgenotypes 14, 15 and 16. Conversely amino acid deletion 197 was restricted to all isolates within a particular genetic cluster. The present work is the first comprehensive phylogenetic analysis of FMDV type A in South America, filling a gap of knowledge with respect to both, historical and acting viruses. The results provided evidence that supports the ecosystem dynamics in the region, and also served as an input to establish genetic links of emergencies in already-declared free areas, highlighting the need for strengthening control activities.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16895
Malirat, Viviana; Bergmann, Ingrid Evelyn; Campos, Renata de Mendonça; Conde, Florangel; Quiroga, José Luis; et al.; Molecular epidemiology of foot-and-mouth disease virus type A in South America; Elsevier Science; Veterinary Microbiology; 158; 1-2; 7-2012; 82-94
0378-1135
url http://hdl.handle.net/11336/16895
identifier_str_mv Malirat, Viviana; Bergmann, Ingrid Evelyn; Campos, Renata de Mendonça; Conde, Florangel; Quiroga, José Luis; et al.; Molecular epidemiology of foot-and-mouth disease virus type A in South America; Elsevier Science; Veterinary Microbiology; 158; 1-2; 7-2012; 82-94
0378-1135
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetmic.2012.02.009
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0378113512001198
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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