Control of cell growth direction by direct fabrication of periodic micro- and submicrometer arrays on polymers

Autores
Langheinrich, Denise; Yslas, Edith Inés; Broglia, Martin Federico; Rivarola, Viviana; Acevedo, Diego Fernando; Lasagni, Andrés
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, we describe a laser-assisted microstructuring technique called Direct Laser Interference Patterning to produce topographical cues for tumor cells in a one-step process. Line-like patterns with spatial periods ranging from 500 nm to 10 μm are fabricated on polyimide (PI) films. The resulting structures exhibit a well-defined shape and quality even for patterns with small periodic distances. Subsequently, the behavior of mouse mammary adenocarcinoma cells over those structures is evaluated. The results show that cell growth is well aligned to the direction of the patterns (over 60% lying within 0° to 15°to either side of the surface lines) for all evaluated structure sizes. Moreover, cells grown on patterns with 500 nm spatial period are the most narrowly aligned (up to 80% found between 0°and 15°), showing the potential of the technique. The fabrication process of the PI patterns is supported by a mathematical model of the underlying photo-chemical ablation process.
Fil: Langheinrich, Denise. Fraunhofer Institute for Material and Beam Technology; Alemania
Fil: Yslas, Edith Inés. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Broglia, Martin Federico. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Rivarola, Viviana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Acevedo, Diego Fernando. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Lasagni, Andrés. Fraunhofer Institute for Material and Beam Technology; Alemania
Materia
BIOMATERIALS
CELL GUIDANCE
DIRECT LASER INTERFERENCE PATTERNING
LASER ABLATION
POLYIMIDE
SURFACES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194963
Acceder
id CONICETDig_48f6ba1c235cb419c91aff561d5ac678
oai_identifier_str oai:ri.conicet.gov.ar:11336/194963
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Control of cell growth direction by direct fabrication of periodic micro- and submicrometer arrays on polymersLangheinrich, DeniseYslas, Edith InésBroglia, Martin FedericoRivarola, VivianaAcevedo, Diego FernandoLasagni, AndrésBIOMATERIALSCELL GUIDANCEDIRECT LASER INTERFERENCE PATTERNINGLASER ABLATIONPOLYIMIDESURFACEShttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2In this work, we describe a laser-assisted microstructuring technique called Direct Laser Interference Patterning to produce topographical cues for tumor cells in a one-step process. Line-like patterns with spatial periods ranging from 500 nm to 10 μm are fabricated on polyimide (PI) films. The resulting structures exhibit a well-defined shape and quality even for patterns with small periodic distances. Subsequently, the behavior of mouse mammary adenocarcinoma cells over those structures is evaluated. The results show that cell growth is well aligned to the direction of the patterns (over 60% lying within 0° to 15°to either side of the surface lines) for all evaluated structure sizes. Moreover, cells grown on patterns with 500 nm spatial period are the most narrowly aligned (up to 80% found between 0°and 15°), showing the potential of the technique. The fabrication process of the PI patterns is supported by a mathematical model of the underlying photo-chemical ablation process.Fil: Langheinrich, Denise. Fraunhofer Institute for Material and Beam Technology; AlemaniaFil: Yslas, Edith Inés. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Broglia, Martin Federico. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Rivarola, Viviana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Acevedo, Diego Fernando. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Lasagni, Andrés. Fraunhofer Institute for Material and Beam Technology; AlemaniaJohn Wiley & Sons2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194963Langheinrich, Denise; Yslas, Edith Inés; Broglia, Martin Federico; Rivarola, Viviana; Acevedo, Diego Fernando; et al.; Control of cell growth direction by direct fabrication of periodic micro- and submicrometer arrays on polymers; John Wiley & Sons; Journal of Polymer Science Part B: Polymer Physics; 50; 6; 3-2012; 415-4220887-6266CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/polb.23017info:eu-repo/semantics/altIdentifier/doi/10.1002/polb.23017info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:55Zoai:ri.conicet.gov.ar:11336/194963instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:55.381CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Control of cell growth direction by direct fabrication of periodic micro- and submicrometer arrays on polymers
title Control of cell growth direction by direct fabrication of periodic micro- and submicrometer arrays on polymers
spellingShingle Control of cell growth direction by direct fabrication of periodic micro- and submicrometer arrays on polymers
Langheinrich, Denise
BIOMATERIALS
CELL GUIDANCE
DIRECT LASER INTERFERENCE PATTERNING
LASER ABLATION
POLYIMIDE
SURFACES
title_short Control of cell growth direction by direct fabrication of periodic micro- and submicrometer arrays on polymers
title_full Control of cell growth direction by direct fabrication of periodic micro- and submicrometer arrays on polymers
title_fullStr Control of cell growth direction by direct fabrication of periodic micro- and submicrometer arrays on polymers
title_full_unstemmed Control of cell growth direction by direct fabrication of periodic micro- and submicrometer arrays on polymers
title_sort Control of cell growth direction by direct fabrication of periodic micro- and submicrometer arrays on polymers
dc.creator.none.fl_str_mv Langheinrich, Denise
Yslas, Edith Inés
Broglia, Martin Federico
Rivarola, Viviana
Acevedo, Diego Fernando
Lasagni, Andrés
author Langheinrich, Denise
author_facet Langheinrich, Denise
Yslas, Edith Inés
Broglia, Martin Federico
Rivarola, Viviana
Acevedo, Diego Fernando
Lasagni, Andrés
author_role author
author2 Yslas, Edith Inés
Broglia, Martin Federico
Rivarola, Viviana
Acevedo, Diego Fernando
Lasagni, Andrés
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIOMATERIALS
CELL GUIDANCE
DIRECT LASER INTERFERENCE PATTERNING
LASER ABLATION
POLYIMIDE
SURFACES
topic BIOMATERIALS
CELL GUIDANCE
DIRECT LASER INTERFERENCE PATTERNING
LASER ABLATION
POLYIMIDE
SURFACES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv In this work, we describe a laser-assisted microstructuring technique called Direct Laser Interference Patterning to produce topographical cues for tumor cells in a one-step process. Line-like patterns with spatial periods ranging from 500 nm to 10 μm are fabricated on polyimide (PI) films. The resulting structures exhibit a well-defined shape and quality even for patterns with small periodic distances. Subsequently, the behavior of mouse mammary adenocarcinoma cells over those structures is evaluated. The results show that cell growth is well aligned to the direction of the patterns (over 60% lying within 0° to 15°to either side of the surface lines) for all evaluated structure sizes. Moreover, cells grown on patterns with 500 nm spatial period are the most narrowly aligned (up to 80% found between 0°and 15°), showing the potential of the technique. The fabrication process of the PI patterns is supported by a mathematical model of the underlying photo-chemical ablation process.
Fil: Langheinrich, Denise. Fraunhofer Institute for Material and Beam Technology; Alemania
Fil: Yslas, Edith Inés. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Broglia, Martin Federico. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Rivarola, Viviana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Acevedo, Diego Fernando. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Lasagni, Andrés. Fraunhofer Institute for Material and Beam Technology; Alemania
description In this work, we describe a laser-assisted microstructuring technique called Direct Laser Interference Patterning to produce topographical cues for tumor cells in a one-step process. Line-like patterns with spatial periods ranging from 500 nm to 10 μm are fabricated on polyimide (PI) films. The resulting structures exhibit a well-defined shape and quality even for patterns with small periodic distances. Subsequently, the behavior of mouse mammary adenocarcinoma cells over those structures is evaluated. The results show that cell growth is well aligned to the direction of the patterns (over 60% lying within 0° to 15°to either side of the surface lines) for all evaluated structure sizes. Moreover, cells grown on patterns with 500 nm spatial period are the most narrowly aligned (up to 80% found between 0°and 15°), showing the potential of the technique. The fabrication process of the PI patterns is supported by a mathematical model of the underlying photo-chemical ablation process.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194963
Langheinrich, Denise; Yslas, Edith Inés; Broglia, Martin Federico; Rivarola, Viviana; Acevedo, Diego Fernando; et al.; Control of cell growth direction by direct fabrication of periodic micro- and submicrometer arrays on polymers; John Wiley & Sons; Journal of Polymer Science Part B: Polymer Physics; 50; 6; 3-2012; 415-422
0887-6266
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194963
identifier_str_mv Langheinrich, Denise; Yslas, Edith Inés; Broglia, Martin Federico; Rivarola, Viviana; Acevedo, Diego Fernando; et al.; Control of cell growth direction by direct fabrication of periodic micro- and submicrometer arrays on polymers; John Wiley & Sons; Journal of Polymer Science Part B: Polymer Physics; 50; 6; 3-2012; 415-422
0887-6266
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/polb.23017
info:eu-repo/semantics/altIdentifier/doi/10.1002/polb.23017
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613818169163776
score 13.070432

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