Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts

Autores
Le, Phuong Thi; Makhalanyane, Thulani P.; Guerrero, Leandro Demián; Vikram, Surendra; Van De Peer, Yves; Cowan, Don A.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Understanding microbial adaptation to environmental stressors is crucial for interpreting broader ecological patterns. In the most extreme hot and cold deserts, cryptic niche communities are thought to play key roles in ecosystem processes and represent excellent model systems for investigating microbial responses to environmental stressors. However, relatively little is known about the genetic diversity underlying such functional processes in climatically extreme desert systems. This study presents the first comparative metagenome analysis of cyanobacteria-dominated hypolithic communities in hot (Namib Desert, Namibia) and cold (Miers Valley, Antarctica) hyperarid deserts. The most abundant phyla in both hypolith metagenomes were Actinobacteria, Proteobacteria, Cyanobacteria and Bacteroidetes with Cyanobacteria dominating in Antarctic hypoliths. However, no significant differences between the twometagenomeswere identified. The Antarctic hypolithicmetagenome displayed a high number of sequences assigned to sigma factors, replication,recombination andrepair, translation, ribosomal structure,andbiogenesis. In contrast, theNamibDesert metagenome showed a high abundance of sequences assigned to carbohydrate transport and metabolism. Metagenome data analysis also revealed significantdivergence inthe geneticdeterminantsof aminoacidandnucleotidemetabolismbetween these two metagenomes and those of soil from other polar deserts, hot deserts, and non-desert soils. Our results suggest extensive niche differentiation in hypolithic microbial communities from these two extreme environments and a high genetic capacity for survival under environmental extremes.
Fil: Le, Phuong Thi. University of Pretoria; Sudáfrica. Vlaams Instituut voor Biotechnologie; Bélgica. University of Ghent; Bélgica
Fil: Makhalanyane, Thulani P.. University of Pretoria; Sudáfrica
Fil: Guerrero, Leandro Demián. University of Pretoria; Sudáfrica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Vikram, Surendra. University of Pretoria; Sudáfrica
Fil: Van De Peer, Yves. University of Pretoria; Sudáfrica. Vlaams Instituut voor Biotechnologie; Bélgica. University of Ghent; Bélgica
Fil: Cowan, Don A.. University of Pretoria; Sudáfrica
Materia
ANTARCTICA
BIOMES
COMPARATIVE METAGENOMICS
DESERTS
HYPOLITHS
NAMIB DESERT
SOILS
STRESS RESPONSE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79410
Acceder
id CONICETDig_4833f8801af36ebf6fb972668974a869
oai_identifier_str oai:ri.conicet.gov.ar:11336/79410
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid desertsLe, Phuong ThiMakhalanyane, Thulani P.Guerrero, Leandro DemiánVikram, SurendraVan De Peer, YvesCowan, Don A.ANTARCTICABIOMESCOMPARATIVE METAGENOMICSDESERTSHYPOLITHSNAMIB DESERTSOILSSTRESS RESPONSEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Understanding microbial adaptation to environmental stressors is crucial for interpreting broader ecological patterns. In the most extreme hot and cold deserts, cryptic niche communities are thought to play key roles in ecosystem processes and represent excellent model systems for investigating microbial responses to environmental stressors. However, relatively little is known about the genetic diversity underlying such functional processes in climatically extreme desert systems. This study presents the first comparative metagenome analysis of cyanobacteria-dominated hypolithic communities in hot (Namib Desert, Namibia) and cold (Miers Valley, Antarctica) hyperarid deserts. The most abundant phyla in both hypolith metagenomes were Actinobacteria, Proteobacteria, Cyanobacteria and Bacteroidetes with Cyanobacteria dominating in Antarctic hypoliths. However, no significant differences between the twometagenomeswere identified. The Antarctic hypolithicmetagenome displayed a high number of sequences assigned to sigma factors, replication,recombination andrepair, translation, ribosomal structure,andbiogenesis. In contrast, theNamibDesert metagenome showed a high abundance of sequences assigned to carbohydrate transport and metabolism. Metagenome data analysis also revealed significantdivergence inthe geneticdeterminantsof aminoacidandnucleotidemetabolismbetween these two metagenomes and those of soil from other polar deserts, hot deserts, and non-desert soils. Our results suggest extensive niche differentiation in hypolithic microbial communities from these two extreme environments and a high genetic capacity for survival under environmental extremes.Fil: Le, Phuong Thi. University of Pretoria; Sudáfrica. Vlaams Instituut voor Biotechnologie; Bélgica. University of Ghent; BélgicaFil: Makhalanyane, Thulani P.. University of Pretoria; SudáfricaFil: Guerrero, Leandro Demián. University of Pretoria; Sudáfrica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Vikram, Surendra. University of Pretoria; SudáfricaFil: Van De Peer, Yves. University of Pretoria; Sudáfrica. Vlaams Instituut voor Biotechnologie; Bélgica. University of Ghent; BélgicaFil: Cowan, Don A.. University of Pretoria; SudáfricaOxford University Press2016-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79410Le, Phuong Thi; Makhalanyane, Thulani P.; Guerrero, Leandro Demián; Vikram, Surendra; Van De Peer, Yves; et al.; Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts; Oxford University Press; Genome Biology and Evolution; 8; 9; 8-2016; 2737-27471759-6653CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/gbe/article/8/9/2737/2236390info:eu-repo/semantics/altIdentifier/doi/10.1093/gbe/evw189info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:53Zoai:ri.conicet.gov.ar:11336/79410instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:53.953CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts
title Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts
spellingShingle Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts
Le, Phuong Thi
ANTARCTICA
BIOMES
COMPARATIVE METAGENOMICS
DESERTS
HYPOLITHS
NAMIB DESERT
SOILS
STRESS RESPONSE
title_short Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts
title_full Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts
title_fullStr Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts
title_full_unstemmed Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts
title_sort Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts
dc.creator.none.fl_str_mv Le, Phuong Thi
Makhalanyane, Thulani P.
Guerrero, Leandro Demián
Vikram, Surendra
Van De Peer, Yves
Cowan, Don A.
author Le, Phuong Thi
author_facet Le, Phuong Thi
Makhalanyane, Thulani P.
Guerrero, Leandro Demián
Vikram, Surendra
Van De Peer, Yves
Cowan, Don A.
author_role author
author2 Makhalanyane, Thulani P.
Guerrero, Leandro Demián
Vikram, Surendra
Van De Peer, Yves
Cowan, Don A.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ANTARCTICA
BIOMES
COMPARATIVE METAGENOMICS
DESERTS
HYPOLITHS
NAMIB DESERT
SOILS
STRESS RESPONSE
topic ANTARCTICA
BIOMES
COMPARATIVE METAGENOMICS
DESERTS
HYPOLITHS
NAMIB DESERT
SOILS
STRESS RESPONSE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Understanding microbial adaptation to environmental stressors is crucial for interpreting broader ecological patterns. In the most extreme hot and cold deserts, cryptic niche communities are thought to play key roles in ecosystem processes and represent excellent model systems for investigating microbial responses to environmental stressors. However, relatively little is known about the genetic diversity underlying such functional processes in climatically extreme desert systems. This study presents the first comparative metagenome analysis of cyanobacteria-dominated hypolithic communities in hot (Namib Desert, Namibia) and cold (Miers Valley, Antarctica) hyperarid deserts. The most abundant phyla in both hypolith metagenomes were Actinobacteria, Proteobacteria, Cyanobacteria and Bacteroidetes with Cyanobacteria dominating in Antarctic hypoliths. However, no significant differences between the twometagenomeswere identified. The Antarctic hypolithicmetagenome displayed a high number of sequences assigned to sigma factors, replication,recombination andrepair, translation, ribosomal structure,andbiogenesis. In contrast, theNamibDesert metagenome showed a high abundance of sequences assigned to carbohydrate transport and metabolism. Metagenome data analysis also revealed significantdivergence inthe geneticdeterminantsof aminoacidandnucleotidemetabolismbetween these two metagenomes and those of soil from other polar deserts, hot deserts, and non-desert soils. Our results suggest extensive niche differentiation in hypolithic microbial communities from these two extreme environments and a high genetic capacity for survival under environmental extremes.
Fil: Le, Phuong Thi. University of Pretoria; Sudáfrica. Vlaams Instituut voor Biotechnologie; Bélgica. University of Ghent; Bélgica
Fil: Makhalanyane, Thulani P.. University of Pretoria; Sudáfrica
Fil: Guerrero, Leandro Demián. University of Pretoria; Sudáfrica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Vikram, Surendra. University of Pretoria; Sudáfrica
Fil: Van De Peer, Yves. University of Pretoria; Sudáfrica. Vlaams Instituut voor Biotechnologie; Bélgica. University of Ghent; Bélgica
Fil: Cowan, Don A.. University of Pretoria; Sudáfrica
description Understanding microbial adaptation to environmental stressors is crucial for interpreting broader ecological patterns. In the most extreme hot and cold deserts, cryptic niche communities are thought to play key roles in ecosystem processes and represent excellent model systems for investigating microbial responses to environmental stressors. However, relatively little is known about the genetic diversity underlying such functional processes in climatically extreme desert systems. This study presents the first comparative metagenome analysis of cyanobacteria-dominated hypolithic communities in hot (Namib Desert, Namibia) and cold (Miers Valley, Antarctica) hyperarid deserts. The most abundant phyla in both hypolith metagenomes were Actinobacteria, Proteobacteria, Cyanobacteria and Bacteroidetes with Cyanobacteria dominating in Antarctic hypoliths. However, no significant differences between the twometagenomeswere identified. The Antarctic hypolithicmetagenome displayed a high number of sequences assigned to sigma factors, replication,recombination andrepair, translation, ribosomal structure,andbiogenesis. In contrast, theNamibDesert metagenome showed a high abundance of sequences assigned to carbohydrate transport and metabolism. Metagenome data analysis also revealed significantdivergence inthe geneticdeterminantsof aminoacidandnucleotidemetabolismbetween these two metagenomes and those of soil from other polar deserts, hot deserts, and non-desert soils. Our results suggest extensive niche differentiation in hypolithic microbial communities from these two extreme environments and a high genetic capacity for survival under environmental extremes.
publishDate 2016
dc.date.none.fl_str_mv 2016-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79410
Le, Phuong Thi; Makhalanyane, Thulani P.; Guerrero, Leandro Demián; Vikram, Surendra; Van De Peer, Yves; et al.; Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts; Oxford University Press; Genome Biology and Evolution; 8; 9; 8-2016; 2737-2747
1759-6653
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79410
identifier_str_mv Le, Phuong Thi; Makhalanyane, Thulani P.; Guerrero, Leandro Demián; Vikram, Surendra; Van De Peer, Yves; et al.; Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts; Oxford University Press; Genome Biology and Evolution; 8; 9; 8-2016; 2737-2747
1759-6653
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/gbe/article/8/9/2737/2236390
info:eu-repo/semantics/altIdentifier/doi/10.1093/gbe/evw189
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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