High frequencies of telomeric associations, chromosome aberrations, and sister chromatid exchanges in ulcerative colitis

Autores
Cottliar, Alejandra; Fundia, Ariela Freya; Boerr, Luis; Sambuelli, Alicia; Negreira, Silvia; Gil, Anibal; Gomez, Juan Carlos; Chopita, Nestor; Bernedo, Alberto; Slavutsky, Irma Rosa
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chromosome instability provides a predisposing background to malignancy, contributing to the crucial genetic changes in multistep carcinogenesis. The aim of this work was to analyze chromosome instability in patients with ulcerative colitis (UC) to achieve a better understanding of the increased risk for colorectal cancer. METHODS: Peripheral blood lymphocyte cultures from 20 untreated UC patients and 24 controls were used to study chromosome instability by assessing telomeric associations (TAS), chromosome aberrations (CA), and sister chromatid exchanges (SCE). RESULTS: Mean frequencies of TAS and CA were significantly increased in UC patients compared to controls (p < 0.001). Chromosomes 10, 11, 21, 16, and 19 were the most frequently involved in TAS. A total of 104 CA clustered in 66 breakpoints could be exactly localized. Seven nonrandom bands significantly affected in UC patients were found (p < 0.004), showing a significant correlation with the location of cancer breakpoints (p < 0.003), particularly with colorectal carcinoma rearrangements. SCE analysis showed higher levels in patients compared to controls (p < 0.006), but no differences were observed in cell cycle kinetics. CONCLUSIONS: Our results demonstrate the presence of an unstable genome in UC patients that could be related to the cancer development observed in this disease.
Fil: Cottliar, Alejandra. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Fundia, Ariela Freya. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Boerr, Luis. Hospital de Gastroenterología “Bonorino Udaondo,; Argentina
Fil: Sambuelli, Alicia. Hospital de Gastroenterología “Bonorino Udaondo,; Argentina
Fil: Negreira, Silvia. Hospital de Gastroenterología “Bonorino Udaondo,; Argentina
Fil: Gil, Anibal. Hospital de Gastroenterología “Bonorino Udaondo,; Argentina
Fil: Gomez, Juan Carlos. Provincia de Buenos Aires. Hospital Interzonal General de Agudos Gral. San Martin; Argentina
Fil: Chopita, Nestor. Provincia de Buenos Aires. Hospital Interzonal General de Agudos Gral. San Martin; Argentina
Fil: Bernedo, Alberto. Provincia de Buenos Aires. Hospital Interzonal General de Agudos Gral. San Martin; Argentina
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Materia
ULCERATIVE COLITIS
CHROMOSOME ABERRATIONS
TELOMERIC ASSOCIATIONS
SISTER CHROMATID EXCHANGE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55192
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/55192
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling High frequencies of telomeric associations, chromosome aberrations, and sister chromatid exchanges in ulcerative colitisCottliar, AlejandraFundia, Ariela FreyaBoerr, LuisSambuelli, AliciaNegreira, SilviaGil, AnibalGomez, Juan CarlosChopita, NestorBernedo, AlbertoSlavutsky, Irma RosaULCERATIVE COLITISCHROMOSOME ABERRATIONSTELOMERIC ASSOCIATIONSSISTER CHROMATID EXCHANGEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Chromosome instability provides a predisposing background to malignancy, contributing to the crucial genetic changes in multistep carcinogenesis. The aim of this work was to analyze chromosome instability in patients with ulcerative colitis (UC) to achieve a better understanding of the increased risk for colorectal cancer. METHODS: Peripheral blood lymphocyte cultures from 20 untreated UC patients and 24 controls were used to study chromosome instability by assessing telomeric associations (TAS), chromosome aberrations (CA), and sister chromatid exchanges (SCE). RESULTS: Mean frequencies of TAS and CA were significantly increased in UC patients compared to controls (p < 0.001). Chromosomes 10, 11, 21, 16, and 19 were the most frequently involved in TAS. A total of 104 CA clustered in 66 breakpoints could be exactly localized. Seven nonrandom bands significantly affected in UC patients were found (p < 0.004), showing a significant correlation with the location of cancer breakpoints (p < 0.003), particularly with colorectal carcinoma rearrangements. SCE analysis showed higher levels in patients compared to controls (p < 0.006), but no differences were observed in cell cycle kinetics. CONCLUSIONS: Our results demonstrate the presence of an unstable genome in UC patients that could be related to the cancer development observed in this disease.Fil: Cottliar, Alejandra. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; ArgentinaFil: Fundia, Ariela Freya. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; ArgentinaFil: Boerr, Luis. Hospital de Gastroenterología “Bonorino Udaondo,; ArgentinaFil: Sambuelli, Alicia. Hospital de Gastroenterología “Bonorino Udaondo,; ArgentinaFil: Negreira, Silvia. Hospital de Gastroenterología “Bonorino Udaondo,; ArgentinaFil: Gil, Anibal. Hospital de Gastroenterología “Bonorino Udaondo,; ArgentinaFil: Gomez, Juan Carlos. Provincia de Buenos Aires. Hospital Interzonal General de Agudos Gral. San Martin; ArgentinaFil: Chopita, Nestor. Provincia de Buenos Aires. Hospital Interzonal General de Agudos Gral. San Martin; ArgentinaFil: Bernedo, Alberto. Provincia de Buenos Aires. Hospital Interzonal General de Agudos Gral. San Martin; ArgentinaFil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; ArgentinaNature Publishing Group2000-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55192Cottliar, Alejandra; Fundia, Ariela Freya; Boerr, Luis; Sambuelli, Alicia; Negreira, Silvia; et al.; High frequencies of telomeric associations, chromosome aberrations, and sister chromatid exchanges in ulcerative colitis; Nature Publishing Group; American Journal Of Gastroenterology; 95; 9; 9-2000; 2301-23070002-9270CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1572-0241.2000.02315.xinfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/ajg2000581info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:00Zoai:ri.conicet.gov.ar:11336/55192instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:00.495CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv High frequencies of telomeric associations, chromosome aberrations, and sister chromatid exchanges in ulcerative colitis
title High frequencies of telomeric associations, chromosome aberrations, and sister chromatid exchanges in ulcerative colitis
spellingShingle High frequencies of telomeric associations, chromosome aberrations, and sister chromatid exchanges in ulcerative colitis
Cottliar, Alejandra
ULCERATIVE COLITIS
CHROMOSOME ABERRATIONS
TELOMERIC ASSOCIATIONS
SISTER CHROMATID EXCHANGE
title_short High frequencies of telomeric associations, chromosome aberrations, and sister chromatid exchanges in ulcerative colitis
title_full High frequencies of telomeric associations, chromosome aberrations, and sister chromatid exchanges in ulcerative colitis
title_fullStr High frequencies of telomeric associations, chromosome aberrations, and sister chromatid exchanges in ulcerative colitis
title_full_unstemmed High frequencies of telomeric associations, chromosome aberrations, and sister chromatid exchanges in ulcerative colitis
title_sort High frequencies of telomeric associations, chromosome aberrations, and sister chromatid exchanges in ulcerative colitis
dc.creator.none.fl_str_mv Cottliar, Alejandra
Fundia, Ariela Freya
Boerr, Luis
Sambuelli, Alicia
Negreira, Silvia
Gil, Anibal
Gomez, Juan Carlos
Chopita, Nestor
Bernedo, Alberto
Slavutsky, Irma Rosa
author Cottliar, Alejandra
author_facet Cottliar, Alejandra
Fundia, Ariela Freya
Boerr, Luis
Sambuelli, Alicia
Negreira, Silvia
Gil, Anibal
Gomez, Juan Carlos
Chopita, Nestor
Bernedo, Alberto
Slavutsky, Irma Rosa
author_role author
author2 Fundia, Ariela Freya
Boerr, Luis
Sambuelli, Alicia
Negreira, Silvia
Gil, Anibal
Gomez, Juan Carlos
Chopita, Nestor
Bernedo, Alberto
Slavutsky, Irma Rosa
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ULCERATIVE COLITIS
CHROMOSOME ABERRATIONS
TELOMERIC ASSOCIATIONS
SISTER CHROMATID EXCHANGE
topic ULCERATIVE COLITIS
CHROMOSOME ABERRATIONS
TELOMERIC ASSOCIATIONS
SISTER CHROMATID EXCHANGE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Chromosome instability provides a predisposing background to malignancy, contributing to the crucial genetic changes in multistep carcinogenesis. The aim of this work was to analyze chromosome instability in patients with ulcerative colitis (UC) to achieve a better understanding of the increased risk for colorectal cancer. METHODS: Peripheral blood lymphocyte cultures from 20 untreated UC patients and 24 controls were used to study chromosome instability by assessing telomeric associations (TAS), chromosome aberrations (CA), and sister chromatid exchanges (SCE). RESULTS: Mean frequencies of TAS and CA were significantly increased in UC patients compared to controls (p < 0.001). Chromosomes 10, 11, 21, 16, and 19 were the most frequently involved in TAS. A total of 104 CA clustered in 66 breakpoints could be exactly localized. Seven nonrandom bands significantly affected in UC patients were found (p < 0.004), showing a significant correlation with the location of cancer breakpoints (p < 0.003), particularly with colorectal carcinoma rearrangements. SCE analysis showed higher levels in patients compared to controls (p < 0.006), but no differences were observed in cell cycle kinetics. CONCLUSIONS: Our results demonstrate the presence of an unstable genome in UC patients that could be related to the cancer development observed in this disease.
Fil: Cottliar, Alejandra. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Fundia, Ariela Freya. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Boerr, Luis. Hospital de Gastroenterología “Bonorino Udaondo,; Argentina
Fil: Sambuelli, Alicia. Hospital de Gastroenterología “Bonorino Udaondo,; Argentina
Fil: Negreira, Silvia. Hospital de Gastroenterología “Bonorino Udaondo,; Argentina
Fil: Gil, Anibal. Hospital de Gastroenterología “Bonorino Udaondo,; Argentina
Fil: Gomez, Juan Carlos. Provincia de Buenos Aires. Hospital Interzonal General de Agudos Gral. San Martin; Argentina
Fil: Chopita, Nestor. Provincia de Buenos Aires. Hospital Interzonal General de Agudos Gral. San Martin; Argentina
Fil: Bernedo, Alberto. Provincia de Buenos Aires. Hospital Interzonal General de Agudos Gral. San Martin; Argentina
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
description Chromosome instability provides a predisposing background to malignancy, contributing to the crucial genetic changes in multistep carcinogenesis. The aim of this work was to analyze chromosome instability in patients with ulcerative colitis (UC) to achieve a better understanding of the increased risk for colorectal cancer. METHODS: Peripheral blood lymphocyte cultures from 20 untreated UC patients and 24 controls were used to study chromosome instability by assessing telomeric associations (TAS), chromosome aberrations (CA), and sister chromatid exchanges (SCE). RESULTS: Mean frequencies of TAS and CA were significantly increased in UC patients compared to controls (p < 0.001). Chromosomes 10, 11, 21, 16, and 19 were the most frequently involved in TAS. A total of 104 CA clustered in 66 breakpoints could be exactly localized. Seven nonrandom bands significantly affected in UC patients were found (p < 0.004), showing a significant correlation with the location of cancer breakpoints (p < 0.003), particularly with colorectal carcinoma rearrangements. SCE analysis showed higher levels in patients compared to controls (p < 0.006), but no differences were observed in cell cycle kinetics. CONCLUSIONS: Our results demonstrate the presence of an unstable genome in UC patients that could be related to the cancer development observed in this disease.
publishDate 2000
dc.date.none.fl_str_mv 2000-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55192
Cottliar, Alejandra; Fundia, Ariela Freya; Boerr, Luis; Sambuelli, Alicia; Negreira, Silvia; et al.; High frequencies of telomeric associations, chromosome aberrations, and sister chromatid exchanges in ulcerative colitis; Nature Publishing Group; American Journal Of Gastroenterology; 95; 9; 9-2000; 2301-2307
0002-9270
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55192
identifier_str_mv Cottliar, Alejandra; Fundia, Ariela Freya; Boerr, Luis; Sambuelli, Alicia; Negreira, Silvia; et al.; High frequencies of telomeric associations, chromosome aberrations, and sister chromatid exchanges in ulcerative colitis; Nature Publishing Group; American Journal Of Gastroenterology; 95; 9; 9-2000; 2301-2307
0002-9270
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1572-0241.2000.02315.x
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/ajg2000581
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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