Natural Sesquiterpene Lactones Enhance Oxacillin and Gentamicin Effectiveness Against Pathogenic Bacteria Without Antibacterial Effects on Beneficial Lactobacilli

Autores
Cartagena, Elena; Alva, Mariana; Montanaro, Susana; Bardon, Alicia del Valle
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This is a report on the synergistic interactions (SIs) between melampolide‐type sesquiterpene lactones 1–8 from Acanthospermum hispidum DC., and oxacillin or gentamicin, against four pathogenic strains of Staphylococcus aureus and Enterococcus faecalis; two of them were multi‐resistant strains obtained from chronic infectious processes. Our results showed that all associations of 1–8 with antibiotics (ATBs) are more effective than pure ATBs to control pathogenic strains of S. aureus and E. faecalis. The most relevant SIs were observed when the major lactone of A. hispidum, acanthospermal B [5], was combined with gentamicin (protein synthesis inhibitor) against an ex vivo culture of methicillin‐resistant S. aureus SAR 1, displaying a significant MIC reduction in 5 (312.5 to 78.1 µg/mL), and gentamicin (120 µg/mL to 3 µg/mL). Compound 4 improved the antibiotic potency of oxacillin (cell wall synthesis inhibitor) against ampicillin‐resistant E. faecalis (60 µg/mL to 1.5 µg/mL). It is important to remark that three beneficial lactobacilli were resistant to 1–8 and their mixtures with gentamicin or oxacillin in effective concentrations against pathogenic bacteria. Synergism between ATBs and phytochemicals is a therapeutically helpful concept to improve ATB efficacy and prevent resistance. The present results show that selective SIs occur between melampolides and gentamicin or oxacillin, and open a new field of research.
Fil: Cartagena, Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Alva, Mariana. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Montanaro, Susana. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Bardon, Alicia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Materia
Enterococcus Faecalis
Sesquiterpene Lactones
Staphylococcus Aureus
Synergistic Interactions
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43653
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/43653
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Natural Sesquiterpene Lactones Enhance Oxacillin and Gentamicin Effectiveness Against Pathogenic Bacteria Without Antibacterial Effects on Beneficial LactobacilliCartagena, ElenaAlva, MarianaMontanaro, SusanaBardon, Alicia del ValleEnterococcus FaecalisSesquiterpene LactonesStaphylococcus AureusSynergistic Interactionshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This is a report on the synergistic interactions (SIs) between melampolide‐type sesquiterpene lactones 1–8 from Acanthospermum hispidum DC., and oxacillin or gentamicin, against four pathogenic strains of Staphylococcus aureus and Enterococcus faecalis; two of them were multi‐resistant strains obtained from chronic infectious processes. Our results showed that all associations of 1–8 with antibiotics (ATBs) are more effective than pure ATBs to control pathogenic strains of S. aureus and E. faecalis. The most relevant SIs were observed when the major lactone of A. hispidum, acanthospermal B [5], was combined with gentamicin (protein synthesis inhibitor) against an ex vivo culture of methicillin‐resistant S. aureus SAR 1, displaying a significant MIC reduction in 5 (312.5 to 78.1 µg/mL), and gentamicin (120 µg/mL to 3 µg/mL). Compound 4 improved the antibiotic potency of oxacillin (cell wall synthesis inhibitor) against ampicillin‐resistant E. faecalis (60 µg/mL to 1.5 µg/mL). It is important to remark that three beneficial lactobacilli were resistant to 1–8 and their mixtures with gentamicin or oxacillin in effective concentrations against pathogenic bacteria. Synergism between ATBs and phytochemicals is a therapeutically helpful concept to improve ATB efficacy and prevent resistance. The present results show that selective SIs occur between melampolides and gentamicin or oxacillin, and open a new field of research.Fil: Cartagena, Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Alva, Mariana. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Montanaro, Susana. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Bardon, Alicia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaJohn Wiley & Sons Ltd2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43653Cartagena, Elena; Alva, Mariana; Montanaro, Susana; Bardon, Alicia del Valle; Natural Sesquiterpene Lactones Enhance Oxacillin and Gentamicin Effectiveness Against Pathogenic Bacteria Without Antibacterial Effects on Beneficial Lactobacilli; John Wiley & Sons Ltd; Phytotherapy Research; 29; 5; 5-2015; 695-7000951-418XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.5301info:eu-repo/semantics/altIdentifier/doi/10.1002/ptr.5301info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:41Zoai:ri.conicet.gov.ar:11336/43653instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:41.795CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Natural Sesquiterpene Lactones Enhance Oxacillin and Gentamicin Effectiveness Against Pathogenic Bacteria Without Antibacterial Effects on Beneficial Lactobacilli
title Natural Sesquiterpene Lactones Enhance Oxacillin and Gentamicin Effectiveness Against Pathogenic Bacteria Without Antibacterial Effects on Beneficial Lactobacilli
spellingShingle Natural Sesquiterpene Lactones Enhance Oxacillin and Gentamicin Effectiveness Against Pathogenic Bacteria Without Antibacterial Effects on Beneficial Lactobacilli
Cartagena, Elena
Enterococcus Faecalis
Sesquiterpene Lactones
Staphylococcus Aureus
Synergistic Interactions
title_short Natural Sesquiterpene Lactones Enhance Oxacillin and Gentamicin Effectiveness Against Pathogenic Bacteria Without Antibacterial Effects on Beneficial Lactobacilli
title_full Natural Sesquiterpene Lactones Enhance Oxacillin and Gentamicin Effectiveness Against Pathogenic Bacteria Without Antibacterial Effects on Beneficial Lactobacilli
title_fullStr Natural Sesquiterpene Lactones Enhance Oxacillin and Gentamicin Effectiveness Against Pathogenic Bacteria Without Antibacterial Effects on Beneficial Lactobacilli
title_full_unstemmed Natural Sesquiterpene Lactones Enhance Oxacillin and Gentamicin Effectiveness Against Pathogenic Bacteria Without Antibacterial Effects on Beneficial Lactobacilli
title_sort Natural Sesquiterpene Lactones Enhance Oxacillin and Gentamicin Effectiveness Against Pathogenic Bacteria Without Antibacterial Effects on Beneficial Lactobacilli
dc.creator.none.fl_str_mv Cartagena, Elena
Alva, Mariana
Montanaro, Susana
Bardon, Alicia del Valle
author Cartagena, Elena
author_facet Cartagena, Elena
Alva, Mariana
Montanaro, Susana
Bardon, Alicia del Valle
author_role author
author2 Alva, Mariana
Montanaro, Susana
Bardon, Alicia del Valle
author2_role author
author
author
dc.subject.none.fl_str_mv Enterococcus Faecalis
Sesquiterpene Lactones
Staphylococcus Aureus
Synergistic Interactions
topic Enterococcus Faecalis
Sesquiterpene Lactones
Staphylococcus Aureus
Synergistic Interactions
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This is a report on the synergistic interactions (SIs) between melampolide‐type sesquiterpene lactones 1–8 from Acanthospermum hispidum DC., and oxacillin or gentamicin, against four pathogenic strains of Staphylococcus aureus and Enterococcus faecalis; two of them were multi‐resistant strains obtained from chronic infectious processes. Our results showed that all associations of 1–8 with antibiotics (ATBs) are more effective than pure ATBs to control pathogenic strains of S. aureus and E. faecalis. The most relevant SIs were observed when the major lactone of A. hispidum, acanthospermal B [5], was combined with gentamicin (protein synthesis inhibitor) against an ex vivo culture of methicillin‐resistant S. aureus SAR 1, displaying a significant MIC reduction in 5 (312.5 to 78.1 µg/mL), and gentamicin (120 µg/mL to 3 µg/mL). Compound 4 improved the antibiotic potency of oxacillin (cell wall synthesis inhibitor) against ampicillin‐resistant E. faecalis (60 µg/mL to 1.5 µg/mL). It is important to remark that three beneficial lactobacilli were resistant to 1–8 and their mixtures with gentamicin or oxacillin in effective concentrations against pathogenic bacteria. Synergism between ATBs and phytochemicals is a therapeutically helpful concept to improve ATB efficacy and prevent resistance. The present results show that selective SIs occur between melampolides and gentamicin or oxacillin, and open a new field of research.
Fil: Cartagena, Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Alva, Mariana. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Montanaro, Susana. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Bardon, Alicia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
description This is a report on the synergistic interactions (SIs) between melampolide‐type sesquiterpene lactones 1–8 from Acanthospermum hispidum DC., and oxacillin or gentamicin, against four pathogenic strains of Staphylococcus aureus and Enterococcus faecalis; two of them were multi‐resistant strains obtained from chronic infectious processes. Our results showed that all associations of 1–8 with antibiotics (ATBs) are more effective than pure ATBs to control pathogenic strains of S. aureus and E. faecalis. The most relevant SIs were observed when the major lactone of A. hispidum, acanthospermal B [5], was combined with gentamicin (protein synthesis inhibitor) against an ex vivo culture of methicillin‐resistant S. aureus SAR 1, displaying a significant MIC reduction in 5 (312.5 to 78.1 µg/mL), and gentamicin (120 µg/mL to 3 µg/mL). Compound 4 improved the antibiotic potency of oxacillin (cell wall synthesis inhibitor) against ampicillin‐resistant E. faecalis (60 µg/mL to 1.5 µg/mL). It is important to remark that three beneficial lactobacilli were resistant to 1–8 and their mixtures with gentamicin or oxacillin in effective concentrations against pathogenic bacteria. Synergism between ATBs and phytochemicals is a therapeutically helpful concept to improve ATB efficacy and prevent resistance. The present results show that selective SIs occur between melampolides and gentamicin or oxacillin, and open a new field of research.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43653
Cartagena, Elena; Alva, Mariana; Montanaro, Susana; Bardon, Alicia del Valle; Natural Sesquiterpene Lactones Enhance Oxacillin and Gentamicin Effectiveness Against Pathogenic Bacteria Without Antibacterial Effects on Beneficial Lactobacilli; John Wiley & Sons Ltd; Phytotherapy Research; 29; 5; 5-2015; 695-700
0951-418X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43653
identifier_str_mv Cartagena, Elena; Alva, Mariana; Montanaro, Susana; Bardon, Alicia del Valle; Natural Sesquiterpene Lactones Enhance Oxacillin and Gentamicin Effectiveness Against Pathogenic Bacteria Without Antibacterial Effects on Beneficial Lactobacilli; John Wiley & Sons Ltd; Phytotherapy Research; 29; 5; 5-2015; 695-700
0951-418X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.5301
info:eu-repo/semantics/altIdentifier/doi/10.1002/ptr.5301
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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