The C-terminal region of Trypanosoma cruzi MASPs is antigenic and secreted via exovesicles

Autores
de Pablos, Luis Miguel; Díaz Lozano, Isabel María; Jercic, Maria Isabel; Quinzada, Markela; Giménez, Maria José; Calabuig, Eva; Espino, Ana Margarita; Schijman, Alejandro Gabriel; Zulantay, Inés; Apt, Werner; Osuna, Antonio
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma cruzi is the etiological agent of Chagas disease, a neglected and emerging tropical disease, endemic to South America and present in non-endemic regions due to human migration. The MASP multigene family is specific to T. cruzi, accounting for 6% of the parasite´s genome and plays a key role in immune evasion. A common feature of MASPs is the presence of two conserved regions: an N-terminal region codifying for signal peptide and a C-terminal (C-term) region, which potentially acts as GPI-addition signal peptide. Our aim was the analysis of the presence of an immune response against the MASP C-term region. We found that this region is highly conserved, released via exovesicles (EVs) and has an associated immune response as revealed by epitope affinity mapping, IFA and inhibition of the complement lysis assays. We also demonstrate the presence of a fast IgM response in Balb/c mice infected with T. cruzi. Our results reveal the presence of non-canonical secreted peptides in EVs, which can subsequently be exposed to the immune system with a potential role in evading immune system targets in the parasite.
Fil: de Pablos, Luis Miguel. Universidad de Granada; España. University of York; Reino Unido
Fil: Díaz Lozano, Isabel María. Universidad de Granada; España
Fil: Jercic, Maria Isabel. Instituto de Salud Publica; Chile
Fil: Quinzada, Markela. Universidad de Panamá; Panamá
Fil: Giménez, Maria José. Hospital Universitari i Politècnic La Fe; España
Fil: Calabuig, Eva. Hospital Universitari i Politècnic La Fe; España
Fil: Espino, Ana Margarita. Universidad de Puerto Rico; Puerto Rico
Fil: Schijman, Alejandro Gabriel. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Zulantay, Inés. Universidad de Chile; Chile
Fil: Apt, Werner. Universidad de Chile; Chile
Fil: Osuna, Antonio. Universidad de Granada; España
Materia
Trypanosoma Cruzi
Exosomes
Masp Gene Family
Antigen
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25250
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/25250
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The C-terminal region of Trypanosoma cruzi MASPs is antigenic and secreted via exovesiclesde Pablos, Luis MiguelDíaz Lozano, Isabel MaríaJercic, Maria IsabelQuinzada, MarkelaGiménez, Maria JoséCalabuig, EvaEspino, Ana MargaritaSchijman, Alejandro GabrielZulantay, InésApt, WernerOsuna, AntonioTrypanosoma CruziExosomesMasp Gene FamilyAntigenhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma cruzi is the etiological agent of Chagas disease, a neglected and emerging tropical disease, endemic to South America and present in non-endemic regions due to human migration. The MASP multigene family is specific to T. cruzi, accounting for 6% of the parasite´s genome and plays a key role in immune evasion. A common feature of MASPs is the presence of two conserved regions: an N-terminal region codifying for signal peptide and a C-terminal (C-term) region, which potentially acts as GPI-addition signal peptide. Our aim was the analysis of the presence of an immune response against the MASP C-term region. We found that this region is highly conserved, released via exovesicles (EVs) and has an associated immune response as revealed by epitope affinity mapping, IFA and inhibition of the complement lysis assays. We also demonstrate the presence of a fast IgM response in Balb/c mice infected with T. cruzi. Our results reveal the presence of non-canonical secreted peptides in EVs, which can subsequently be exposed to the immune system with a potential role in evading immune system targets in the parasite.Fil: de Pablos, Luis Miguel. Universidad de Granada; España. University of York; Reino UnidoFil: Díaz Lozano, Isabel María. Universidad de Granada; EspañaFil: Jercic, Maria Isabel. Instituto de Salud Publica; ChileFil: Quinzada, Markela. Universidad de Panamá; PanamáFil: Giménez, Maria José. Hospital Universitari i Politècnic La Fe; EspañaFil: Calabuig, Eva. Hospital Universitari i Politècnic La Fe; EspañaFil: Espino, Ana Margarita. Universidad de Puerto Rico; Puerto RicoFil: Schijman, Alejandro Gabriel. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Zulantay, Inés. Universidad de Chile; ChileFil: Apt, Werner. Universidad de Chile; ChileFil: Osuna, Antonio. Universidad de Granada; EspañaNature Publishing Group2016-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25250de Pablos, Luis Miguel; Díaz Lozano, Isabel María; Jercic, Maria Isabel; Quinzada, Markela; Giménez, Maria José; et al.; The C-terminal region of Trypanosoma cruzi MASPs is antigenic and secreted via exovesicles; Nature Publishing Group; Scientific Reports; 6; 6-2016; 1-12; 272932045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/srep27293info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep27293info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:23:39Zoai:ri.conicet.gov.ar:11336/25250instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:23:40.056CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The C-terminal region of Trypanosoma cruzi MASPs is antigenic and secreted via exovesicles
title The C-terminal region of Trypanosoma cruzi MASPs is antigenic and secreted via exovesicles
spellingShingle The C-terminal region of Trypanosoma cruzi MASPs is antigenic and secreted via exovesicles
de Pablos, Luis Miguel
Trypanosoma Cruzi
Exosomes
Masp Gene Family
Antigen
title_short The C-terminal region of Trypanosoma cruzi MASPs is antigenic and secreted via exovesicles
title_full The C-terminal region of Trypanosoma cruzi MASPs is antigenic and secreted via exovesicles
title_fullStr The C-terminal region of Trypanosoma cruzi MASPs is antigenic and secreted via exovesicles
title_full_unstemmed The C-terminal region of Trypanosoma cruzi MASPs is antigenic and secreted via exovesicles
title_sort The C-terminal region of Trypanosoma cruzi MASPs is antigenic and secreted via exovesicles
dc.creator.none.fl_str_mv de Pablos, Luis Miguel
Díaz Lozano, Isabel María
Jercic, Maria Isabel
Quinzada, Markela
Giménez, Maria José
Calabuig, Eva
Espino, Ana Margarita
Schijman, Alejandro Gabriel
Zulantay, Inés
Apt, Werner
Osuna, Antonio
author de Pablos, Luis Miguel
author_facet de Pablos, Luis Miguel
Díaz Lozano, Isabel María
Jercic, Maria Isabel
Quinzada, Markela
Giménez, Maria José
Calabuig, Eva
Espino, Ana Margarita
Schijman, Alejandro Gabriel
Zulantay, Inés
Apt, Werner
Osuna, Antonio
author_role author
author2 Díaz Lozano, Isabel María
Jercic, Maria Isabel
Quinzada, Markela
Giménez, Maria José
Calabuig, Eva
Espino, Ana Margarita
Schijman, Alejandro Gabriel
Zulantay, Inés
Apt, Werner
Osuna, Antonio
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trypanosoma Cruzi
Exosomes
Masp Gene Family
Antigen
topic Trypanosoma Cruzi
Exosomes
Masp Gene Family
Antigen
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trypanosoma cruzi is the etiological agent of Chagas disease, a neglected and emerging tropical disease, endemic to South America and present in non-endemic regions due to human migration. The MASP multigene family is specific to T. cruzi, accounting for 6% of the parasite´s genome and plays a key role in immune evasion. A common feature of MASPs is the presence of two conserved regions: an N-terminal region codifying for signal peptide and a C-terminal (C-term) region, which potentially acts as GPI-addition signal peptide. Our aim was the analysis of the presence of an immune response against the MASP C-term region. We found that this region is highly conserved, released via exovesicles (EVs) and has an associated immune response as revealed by epitope affinity mapping, IFA and inhibition of the complement lysis assays. We also demonstrate the presence of a fast IgM response in Balb/c mice infected with T. cruzi. Our results reveal the presence of non-canonical secreted peptides in EVs, which can subsequently be exposed to the immune system with a potential role in evading immune system targets in the parasite.
Fil: de Pablos, Luis Miguel. Universidad de Granada; España. University of York; Reino Unido
Fil: Díaz Lozano, Isabel María. Universidad de Granada; España
Fil: Jercic, Maria Isabel. Instituto de Salud Publica; Chile
Fil: Quinzada, Markela. Universidad de Panamá; Panamá
Fil: Giménez, Maria José. Hospital Universitari i Politècnic La Fe; España
Fil: Calabuig, Eva. Hospital Universitari i Politècnic La Fe; España
Fil: Espino, Ana Margarita. Universidad de Puerto Rico; Puerto Rico
Fil: Schijman, Alejandro Gabriel. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Zulantay, Inés. Universidad de Chile; Chile
Fil: Apt, Werner. Universidad de Chile; Chile
Fil: Osuna, Antonio. Universidad de Granada; España
description Trypanosoma cruzi is the etiological agent of Chagas disease, a neglected and emerging tropical disease, endemic to South America and present in non-endemic regions due to human migration. The MASP multigene family is specific to T. cruzi, accounting for 6% of the parasite´s genome and plays a key role in immune evasion. A common feature of MASPs is the presence of two conserved regions: an N-terminal region codifying for signal peptide and a C-terminal (C-term) region, which potentially acts as GPI-addition signal peptide. Our aim was the analysis of the presence of an immune response against the MASP C-term region. We found that this region is highly conserved, released via exovesicles (EVs) and has an associated immune response as revealed by epitope affinity mapping, IFA and inhibition of the complement lysis assays. We also demonstrate the presence of a fast IgM response in Balb/c mice infected with T. cruzi. Our results reveal the presence of non-canonical secreted peptides in EVs, which can subsequently be exposed to the immune system with a potential role in evading immune system targets in the parasite.
publishDate 2016
dc.date.none.fl_str_mv 2016-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25250
de Pablos, Luis Miguel; Díaz Lozano, Isabel María; Jercic, Maria Isabel; Quinzada, Markela; Giménez, Maria José; et al.; The C-terminal region of Trypanosoma cruzi MASPs is antigenic and secreted via exovesicles; Nature Publishing Group; Scientific Reports; 6; 6-2016; 1-12; 27293
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25250
identifier_str_mv de Pablos, Luis Miguel; Díaz Lozano, Isabel María; Jercic, Maria Isabel; Quinzada, Markela; Giménez, Maria José; et al.; The C-terminal region of Trypanosoma cruzi MASPs is antigenic and secreted via exovesicles; Nature Publishing Group; Scientific Reports; 6; 6-2016; 1-12; 27293
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/srep27293
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep27293
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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