Effect of a sublethal high-pressure homogenization treatment on the fatty acid membrane composition of probiotic lactobacilli

Authors
Tabanelli, G.; Patrignani, Francesca; Gardini, F.; Vinderola, Celso Gabriel; Reinheimer, Jorge Alberto; Grazia, L.; Lanciotti, R.
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
High-pressure homogenization (HPH) has been proposed to be applied directly to lactic acid bacterial cells at sublethal levels to enhance some functional properties. As the principal target of HPH are the cell surface envelope structures, the aim of this work was to study the effect of a HPH treatment, applied at 50 MPa, on cell membrane stress responses of already-known functional strains, isolated from Argentinean products. Specifically, the membrane fatty acid composition of cells before and after the sublethal treatment was investigated, and the results showed that plasma membranes, their level of unsaturation and their composition are involved in response mechanisms adopted by microbial cells when subjected to a sublethal HPH stress. In fact, the data obtained demonstrated that the treatment was able to modify the fatty acid profile of the different strains, although a uniform response was not observed. Further studies are necessary both to elucidate the role of each fatty acid in the cell response mechanisms and to clarify the changes in membrane compositions induced by HPH treatment also in relation to the applicative potential of this technique.
Fil: Tabanelli, G.. Inter-Departmental Centre of Industrial Agri-Food Research (CIRI Agroalimentare); Italia
Fil: Patrignani, Francesca. Universidad de Bologna; Italia
Fil: Gardini, F.. Universidad de Bologna; Italia. Inter-Departmental Centre of Industrial Agri-Food Research (CIRI Agroalimentare); Italia
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Grazia, L.. Universidad de Bologna; Italia. Inter-Departmental Centre of Industrial Agri-Food Research (CIRI Agroalimentare); Italia
Fil: Lanciotti, R.. Universidad de Bologna; Italia. Inter-Departmental Centre of Industrial Agri-Food Research (CIRI Agroalimentare); Italia
Subject
Lactobacillus acidophilus
Lactobacillus paracasei
fatty acid membrane composition
high-pressure homogenization
probiotics
Alimentos y Bebidas
Otras Ingenierías y Tecnologías
INGENIERÍAS Y TECNOLOGÍAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/23158